[毕业设计精品] 基于微型光谱仪对水体中氨氮的实时在线检测.doc

本科学生毕业设计（论文）基于微型光谱仪对水体中氨氮的实时在线检测学生：学号：指导教师：教授专业：制药工程化学化工学院二O一一年六月本科学生毕业设计（论文）摘要I摘要环境保护逐渐成为当今社会的一个焦点问题，水质的检测又是环境保护的一个重要组成部分。需样量大，费时，操作繁琐是传统的水质监测技术的突出不足，不便于进行水质的在线监测。微型光谱仪在线检测技术是新兴的技术，具有方便，快捷，高效，样品用量少等特点，可以实现水中多种污染物的快速检测，是未来化学分析技术的一种重要方法，具有非常广阔的发展空间。本文利用分光光度法快速检测水中氨氮含量，并实现水质的实时、在线检测。在研究水杨酸分光光度法(GB/T8538-1995)的基础上，通过使用二氯乙腈尿酸钠代替标准中所用的次氯酸钠，使用柠檬酸三钠作为掩蔽剂，调节体系PH三个方面的研究探讨，对上述因素在氨氮含量检测中的影响进行表征和优化，得到这些影响因素的最优值，探索出一套以紫外可见分光光度计为检测手段，在10min内得到水样中氨氮含量结果的检测方法。对实验条件进行优化后，运用此方法将氨氮的标准样品在自主研发的基于MS-II微型光谱仪的多参数水质监测仪上进行检测，作出标准曲线。多参数水质监测仪检测模拟水样结果与岛津UV2450紫外分光光度计检测结果进行对比，结果显示，多参数水质监测仪的检测结果和岛津UV2450基本一致，误差小于±5%选用二氯乙腈尿酸钠代替次氯酸钠，二氯乙腈尿酸钠的体系浓度为0.313mmol/L，加入量为0.2ml。反应温度维持在30（±0.5），用氢氧化钠调节体系PH值为13左右。反应时间为10min。在上述最佳检测条件下，运用岛津UV2450紫外分光光度仪，得出氨氮含量在0-0.5mg/L浓度范围内的标准曲线为y=0.4423x-0.0163，R2=0.9984，检测灵敏度为0.4423，回收率为99.49%，RSD为1.24%,最低检出限为0.0087mg/L。测得模拟水样中氨氮的含量为0.151mg/L（理论值为0.150mg/L）。运用微型光谱仪在同样的条件和环境下，绘制出氨氮含量在0-0.5mg/L浓度范围内所得标准曲线为y=0.8626x-0.087，R2=0.9969，检测灵敏度为0.8626，回收率为99.36%，RSD为1.34%,最低检出限为0.0045mg/L。测得水样中氨氮含量为0.149mg/L（理论值为0.150mg/L）。关键词:氨氮快速检测，紫外可见分光光度法，微型光谱仪本科学生毕业设计（论文）ABSTRACTIIABSTRACTEnvironmentalprotectionisanimportantissueinmoderntimes.Waterqualitytestingisalsoanimportantissueintheenvironmentalprotection.Thetraditionaltechnologyneedsmuchwatersample,alotoftimeandcumbersomeoperationswhichdonotallowforonlinemonitoringofwaterquality.Micro-detectortechnologyisanemergingtechnology,withtheadvantagesofconvenient,fastoperationandlowwatersampleaddittion,whichcandetectthecontentofinvariouspollutantsinwater.Itisanimportantmeansinchemicalanalysistechnologyinthefuturewhichhasaverybroaddevelopingspace.Thispaperusingspectrophotometryfastdetectionwaterammonianitrogencontent,andrealizethereal-time,waterqualityon-linedetection.Inresearchsalicylicacidspectrophotometricmethod(GB/T8538-1995)basis,throughtheuseofmethylenechlorideacetonitrileuricacidsodiumhypochloriteinsteadofstandardbyusingcitricacid,sodiumasthreemaskingagent,regulatethesystemfromthreeaspectsofthePHoftheabovefactors,andstudytheammonianitrogencontentinthedetectionofinfluence,andoptimizecharacterizedgettheseinfluencefactors,exploretheoptimalvalueofasetofwithuv-visspectrophotometerfor10mindetectionmeans,getinthewaterintheammonianitrogencontentdetectionmethods.Optimizedforexperimentalconditions,usingthismethodwillammonianitrogeninthestandardsamplesindependentlydevelopedbasedonMS-IIminiaturespectrometeronwaterqualitymonitoringmulti-parametertesting,makestandardcurve.Waterqualitymonitoringmulti-parametertestingsimulationresultsandtheislandUV2450watercyanazineultravioletspectrophotometerdetectionresultsarecompared,theresultsshowedthatthewaterqualitymonitoringmulti-parametertestingresultsandislandUV2450jinisbasicallythesameerrorlessthan±5%.Choosetwochlorineacetonitrileuricacidsodiumhypochlorite,insteadofmethylenechlorideacetonitrileuricacidsodiumsystemfor0.313mmol/Lconcentrationof0.2ml,additionamount.Reactiontemperaturemaintainat30,(±0.5)withsodiumhydroxideregulationsystemforabout13PH.Thereactiontimefor10min.Inthebesttestconditions,usingislandUV2450uv-spectrophotometrycyanazineammonianitrogencontentthatinstrument,at0to0.5mg/Lconcentrationrangeofstandardcurveisy=0.4423x-0.0163R2=0.9984detectionsensitivityfor0.4423,recoveryis99.49%,RSD1.24%,lowestdetectlimitfor0.0087mg/L.Watermeasuredinthe本科学生毕业设计（论文）ABSTRACTIIIsimulationofammonianitrogencontentof0.151mg/L(theoretical0.150mg/L)for.Usingthesametermsinminiaturespectrometerandenvironmentandrenderingtheammonianitrogencontentin0-0.5mg/Lconcentrationrangeincomestandardcurveisy=0.8626x-0.087R2=0.9969detectionsensitivityfor0.8626,recoveryis99.36%,RSD1.34%,lowestdetectlimitfor0.0045mg/L.Measuredinammonianitrogencontentinwaterfor0.149mg/L(theoretical0.150mg/L).Keywords:Ammonianitrogenfastdetection,spectrophotometry,MOEMS