欢迎来到人人文库网! | 帮助中心 人人文库renrendoc.com美如初恋!
人人文库网
首页 人人文库网 > 资源分类 > PDF文档下载

外文翻译--关于重铸合金镍铬牙科铸造合金中元素的释放和细胞毒性的体外分析 英文版【优秀】.pdf

  • 资源大小:467.02KB        全文页数:7页
  • 资源格式: PDF        下载权限:游客/注册会员/VIP会员    下载费用:10
游客快捷下载 游客一键下载
会员登录下载
下载资源需要10

邮箱/手机号:
您支付成功后,系统会自动为您创建此邮箱/手机号的账号,密码跟您输入的邮箱/手机号一致,以方便您下次登录下载和查看订单。注:支付完成后需要自己下载文件,并不会自动发送文件哦!

支付方式: 微信支付    支付宝   
验证码:   换一换

友情提示
2、本站资源不支持迅雷下载,请使用浏览器直接下载(不支持QQ浏览器)
3、本站资源下载后的文档和图纸-无水印,预览文档经过压缩,下载后原文更清晰   

外文翻译--关于重铸合金镍铬牙科铸造合金中元素的释放和细胞毒性的体外分析 英文版【优秀】.pdf

ORIGINALARTICLEANINVITROANALYSISOFELEMENTALRELEASEANDCYTOTOXICITYOFRECASTNICKEL–CHROMIUMDENTALCASTINGALLOYSNAGAMRAJAREDDYANANDAPANDIANPONSEKARABRAHAMKRISHNANMURUGESANVASANTHAKUMARMATSARECEIVED30DECEMBER2010/ACCEPTED22MAY2011/PUBLISHEDONLINE3JUNE2011C211INDIANPROSTHODONTICSOCIETY2011ABSTRACTRECASTINGOFTHECASTINGALLOYSAFFECTSTHECOMPOSITIONANDELEMENTALRELEASEWHICHMAYHAVECYTOTOXICEFFECTDIFFERENTFROMTHEPUREALLOYINTHESURROUNDINGTISSUESANINVITROSTUDYWASCONDUCTEDTOINVESTIGATETHEELEMENTALRELEASEANDTHEIRCYTOTOXICEFFECTSFROMCOMMERCIALLYAVAILABLENI–CRDENTALCASTINGALLOYS,COMMONLYUSEDFORFABRICATINGFIXEDPARTIALDENTURESTHREENI–CRALLOYSWIRON99A,CERAMETB,ANDHINICKELCBCWERETESTEDALLOYSPECIMENSDISKS395MMWERECASTEDANDGROUPEDASFOLLOWSGROUPIA1/B1/C1100PUREALLOY;GROUPIIA2/B2/C250NEWWITH50RECAST;ANDGROUPIIIA3/B3/C3100RECASTDISKSOFEACHALLOYTYPEFROMEACHGROUPWERETRANSFERREDTODULBECCO’SMODIFIEDEAGLEMEDIUMANDLEFTFOR3DAYSAT37C176CINANATMOSPHEREOF5CO2NI,CR,CO,CUANDMOELEMENTALRELEASEFROMMETALALLOYSINTOCULTUREMEDIUMWASINVESTIGATEDUSINGINDUCTIVELYCOUPLEDPLASMAMASSSPECTROMETRYCYTOTOXICITYWASTESTEDUSINGMOUSEFIBROBLASTCELLSANDMTTASSAYCONTROLSCONSISTEDOF6WELLSCONTAININGCELLSWITHNOALLOYSPECIMENSDATAWEREANALYZEDBYTWOWAYANALYSISOFVARIANCEFOLLOWEDBYTTESTTHETOTALAMOUNTOFELEMENTSRELEASEDINPARTSPERBILLIONFORVARIOUSCASTINGGROUPSWEREGROUPI,A16572,B16732,C18407;GROUPII,A222046,B226450,C229189;GROUPIII,A384554,B388359,C392264MOREAMOUNTSOFELEMENTSWERERELEASEDINHINICKELCBTHANCERAMETANDWIRON99INALLTHETHREETESTGROUPSPERCENTAGEOFVIABLECELLSFROMMTTANALYSISWEREGROUPI,A162342,B161322C160593,GROUPII,A258699,B256494,C252688,GROUPIII,A353101,B352195,C347586THEVIABLECELLSPRESENTINTHECULTUREMEDIAWEREMOREINWIRON99THANCERAMETANDHINICKELCBELEMENTALRELEASEINCREASEDWITHAMOUNTOFRECASTALLOYAMONGSTTHETHREEALLOYSTESTEDHINICKELCBHADSIGNIFICANTLYHIGHERELEMENTSRELEASEDCOMPAREDTOCERAMETANDWIRON99IN100PUREALLOYS,50RECASTAND100RECASTALLOYSWIRON99SHOWEDLEASTELEMENTRELEASEIN100PUREALLOY,50RECASTAND100RECASTSPECIMENS100PUREALLOYSOFALLTHREEALLOYSARELESSCYTOTOXIC,BUTTHEIRCYTOTOXICITYISMOREON50AND100RECASTEDALLOYSOUTOFALLTHREEVARIATIONSOFCASTINGWIRON99WASLEASTCYTOTOXIC,FOLLOWEDBYCERAMETANDHINICKELCBRECASTINGOFALLOYSSIGNIFICANTLYINCREASEDTHEELEMENTSRELEASEDANDTHEIRCYTOTOXICITYKEYWORDSRECASTINGC1CYTOTOXICITYC1ELEMENTALRELEASEC1DENTALCASTINGALLOYSC1BASEMETALINTRODUCTIONADVANCESINSCIENCEANDTECHNOLOGYINTHEFIELDOFDENTALMATERIALSCIENCEHAVELEADTOTHEINVENTIONOFBASEMETALALLOYSFORAPPLICATIONINDENTISTRY1INTRODUCTIONOFCHROMEALLOYSHASPROVIDEDANALTERNATIVETOGOLD,WITHTHESERESULTEDINNI–CRANDCO–CRBASEDALLOYSWHICHARECLINICALIMPLICATIONSCASTRESTORATIONSPLACEDINCLOSECONTACTWITHORALTISSUESFORVARIOUSPERIODSOFTIMEMAYELICITLOCALADVERSETISSUEREACTIONSSUCHASGINGIVITISANDPERIODONTITISADJACENTTOTHEMDEGREEOFCYTOTOXICITYANDBIOCOMPATIBILITYOFDENTALCASTINGALLOYSHASBEENRELATEDTOALLOYCOMPOSITIONANDELEMENTSRELEASEDFROMALLOYSINTOSURROUNDINGMEDIUMRECASTINGOFNI–CRALLOYSINCREASESTHEAMOUNTOFELEMENTSRELEASEDANDHENCETHECYTOTOXICPOTENTIALNRREDDYCKSTEJAINSTITUTEOFDENTALSCIENCES,TIRUPATHI,ANDHRAPRADESH,INDIAAPABRAHAMC1KMURUGESANC1VMATSADEPARTMENTOFPROSTHODONTICSANDCROWNBRIDGE,SRMDENTALCOLLEGE,SRMUNIVERSITY,RAMAPURAM,CHENNAI,INDIAEMAILDRPONABEGMAILCOM123JINDIANPROSTHODONTSOCAPRJUNE2011112106–112DOI101007/S1319101100758RENRENDOCCOMCONSIDEREDASECONOMICALLYLESSEXPENSIVEANDALSOHAVETHEREQUIREDQUALITIESANDBIOCOMPATIBILITYFORUSAGEINDENTALRESTORATIVEWORK2,3BASEMETALALLOYSAREPRONETOVARIOUSTYPESOFCORROSIONDEPENDINGONITSCOMPOSITIONANDORALENVIRONMENT1THERELEASEOFMETALLICELEMENTSFROMDENTALALLOYSISAPOTENTIALHEALTHPROBLEMTODENTALPATIENT4METALSAREKNOWNTOCAUSETOXICINFLAMMATORYALLERGICORMUTAGENICREACTIONSIMPORTANTCONSEQUENCEOFELEMENTALRELEASEISCYTOTOXICITYOFADJACENTTISSUESINCELLCULTURES5,6CASTRESTORATIONSBEINGPLACEDINCLOSECONTACTWITHORALTISSUESFORVARIOUSPERIODSOFTIMEMAYELICITLOCALADVERSETISSUEREACTIONSSUCHASGINGIVITISANDPERIODONTITISADJACENTTOTHEMDEGREEOFCYTOTOXICITYANDBIOCOMPATIBILITYOFDENTALCASTINGALLOYSHASBEENRELATEDTOALLOYCOMPOSITIONANDELEMENTSRELEASEDFROMALLOYSINTOSURROUNDINGMEDIUM7RECASTINGOFUSEDALLOYSINTHEFORMOFSPRUESORDEFECTIVECASTINGHASBEENPRACTICEDTOPREVENTWASTAGEOFMATERIALAFTERCASTING3IDENTIFICATIONOFTHEELEMENTSANDTHEIRCONCENTRATIONSRESPONSIBLEFORCYTOTOXICEFFECTSISIMPORTANTBECAUSEITWILLHELPINIMPROVINGANDDESIGNINGNEWERALLOYSTOAVOIDTHERELEASEOFELEMENTSWHICHAREHARMFULTOORALCAVITYHYPOTHESISOFTHESTUDYWASTHATRECASTINGOFBASEMETALALLOYSWOULDCHANGETHECHEMICALPROPERTIESOFTHEALLOYSANDTHUSAFFECTTHEIRELEMENTALRELEASEANDSUBSEQUENTLYELICITCYTOTOXICEFFECTSTHEAIMOFTHISINVITROSTUDYWASTOINVESTIGATETHEEFFECTSOFRECASTINGOFNI–CRBASEMETALALLOYSANDTHEIRPOTENTIALCYTOTOXICEFFECTSANDELEMENTALRELEASEINTOCULTUREMEDIAOBJECTIVESOFSTUDYWERETOASSESSTHEELEMENTALRELEASEANDCYTOTOXICITYOFNI–CRALLOYSANDCORRELATEELEMENTALRELEASEWITHCYTOTOXICITYOFPUREALLOYGROUPI,50PUREAND50RECASTEDGROUPIIAND100RECASTEDGROUPIIIALLOYMATERIALSANDMETHODSTHEINVITROSTUDYWASUNDERTAKENWITHNI–CRALLOYFORMETALCERAMICFROMTHREEDIFFERENTMANUFACTURERSFIG1;TABLE1ALLOYSHAVEBEENCODEDFORSIMPLICITYANDREFERENCEPURPOSESPREPARATIONOFALLOYSPECIMENSANALUMINIUMMETALDIEWITHACIRCULARSPACEOF5MMRADIUSAND3MMDEPTHWASMILLEDINTHECENTREPORTIONOF1CMTHICKALUMINIUMBLOCKUSINGCOMPUTERIZEDMILLINGMACHINEFIG2WAXPATTERNS10MMINDIAMETER93MMTHICKNESSWEREPREPAREDWITHGREENINLAYCASTINGWAXFIG3BEGOGERMANYUSINGTHEMETALDIEATOTALOF108WAXPATTERNSWEREMADEFORTHREEALLOYSFORGROUPI100PUREALLOYA1/B1/C1,GROUPII50PURE50RECASTALLOYA2/B2/C2,ANDGROUPIII100RECASTEDALLOYA3/B3/C3CASTINGOFTHEPATTERNTHEWAXPATTERNSOFTHETHREEGROUPSWERESEGREGATEDASGIVENINTABLE2THESPRUEDWAXPATTERNSWEREINVESTEDINPHOSPHATEBONDEDINVESTMENTINMETALCASTINGRINGWITHDRYCELLULOSEPAPERRINGLINERFOLLOWINGTHISTHEYWERECASTEDBYLOSTWAXTECHNIQUEINANINDUCTIONCASTINGMACHINEFIG4BEGOGERMANYWITHTHERESPECTIVEALLOYSMETALSUSEDFORRECASTINGWASOBTAINEDFROMCLEANEDLEFTOVERSPRUESANDBUTTONSOFTHEALLOYSTHATHADBEENPREVIOUSLYCASTINGROUPIAFTERBENCHCOOLINGTHECASTINGRINGWASDIVESTEDANDSANDBLASTEDUSING250LMALUMINIUMOXIDETOREMOVETHEREMNANTINVESTMENTMATERIALSPRUESWERECUTOFFANDSPECIMENSWEREFINISHEDANDPOLISHEDUSINGCARBORUNDUMDISCS,METALTRIMMERS,RUBBERWHEELS,SANDPAPERSANDPOLISHINGCAKEUSINGHANDMOTORINSTRUMENTSTHEPOLISHEDDISKSWERESOAKEDINADETERGENTSOLUTIONFOR5MINANDTHENSCRUBBEDUSINGASOFTBRISTLEBRUSHANDRINSEDUNDERTAPWATERFOR5MINSPECIMENSWERETHENREPLACEDINSTERILEDISTILLEDWATERANDCLEANEDBYSONIFICATIONANDAUTOCLAVEDATATEMPERATUREOF150C176CFOR60MINELEMENTALRELEASESIXDISKSOFEACHALLOYTYPEFROMEACHGROUPWERETRANSFERREDTO6MLOFDULBECCO’SMODIFIEDEAGLEMEDIUMDMEMANDINCUBATEDFOR3DAYSAT37C176CINANATMOSPHEREOF5CO2DMEMISPREPAREDBYSUPPLEMENTINGWITH5NEWBORNCALFSERUM,100U/MLOFPENICILLIN,STREPTOMYCINAND025LGM/LAMPHOTERICINBFIG5THEMEDIAWASANALYZEDFORPRESENCEOFNI,CR,MO,CO,ANDCUUSINGANINDUCTIVELYCOUPLEDPLASMAMASSSPECTROMETRYICPMSMEDIUMWASDILUTEDWITHDEIONIZEDWATERANDKEPTINICPMSANDTHEREADINGSWERERECORDEDTRIPLICATEABSORBANCEWASUSEDTODETERMINETHEMEANCONCENTRATIONOFDIFFERENTELEMENTSINPARTSPERBILLIONPPBRELEASEDFROMALLOYSFIG1NI–CRALLOYSFORMETALCERAMICJINDIANPROSTHODONTSOCAPRJUNE2011112106–112107123RENRENDOCCOMCELLCULTUREANDCYTOTOXICITYTESTFORCYTOTOXICITYTEST,THEREMAINING6DISKSFROMVARIOUSCASTINGALLOYSINTHREEGROUPSWEREPLACEDINCENTEROFA24WELLTISSUECULTUREPLATEAND05MLOFMOUSEFIBROBLASTCELLSUSPENSIONWASADDEDTOCELLWELLFIG6ANDWERELEFTFOR2HFOLLOWINGTHIS05MLOFDMEMWASADDEDANDCELLSWEREINCUBATEDFOR3DAYSAT37C176CINANATMOSPHEREOF5CO2THECONTROLSCONSISTEDOF6WELLSCONTAININGCELLSWITHNOALLOYSPECIMENSAFTERTHEINCUBATIONPERIODTHEPLATESWEREREMOVEDFROMINCUBATORANDCYTOTOXICITYOFTABLE1DESCRIPTIONOFNI–CRDENTALCASTALLOYSCOMPOSITIONASPROVIDEDBYMANUFACTURERSERIALNOTRADENAMEMANUFACTURERCOMPOSITIONWTCODE1WIRON99BEGOGERMANYNICKEL65ACHROMIUM225MOLYBDENUM95NIOBIUM1SILICA1IRON05CESIUM052CERAMETLABOLINEEUROPENICKEL62BCHROMIUM26MOLYBDENUM10SILICA15OTHERS053HINICKELCBHIKARIJAPANNICKEL60CCHROMIUM10COPPER15MANGANESE79OTHERS71FIG2MILLEDDIEFIG3WAXPATTERNSFIG4CASTSPECIMENSTABLE2DISTRIBUTIONOFSPECIMENSGROUPTYPENOOFALLOYSPECIMENAWIRON99BCERAMETCHINICKELCBI100NEW12A112B112C1II50NEW–50RECAST12A212B212C2III100RECAST12A312B312C3108JINDIANPROSTHODONTSOCAPRJUNE2011112106–112123RENRENDOCCOMMATERIALSWASASSESSEDBYMEASURINGSUCCINICDEHYDROGENASEACTIVITYSDHOFTHECELLSBYUSINGMTTASSAYMTTREAGENTISADDEDTOTHECULTUREPLATEWITHALLOYSPECIMENSANDITISTRANSFERREDTO96WELLPLATESFORREADINGSINENZYMELINKEDIMMUNOSORBENTASSAYELISAREADER,THEPROTOCOLFOLLOWEDFORTHEMTTASSAYISSUMMARIZEDINTABLE3THEREADINGSWERECALCULATEDBYTHEFORMULACELLVIABILITYMEANABSORBANCEOFSAMPLEC2100MEANABSORBANCEOFCONTROLSTATISTICALANALYSISTHERESULTSOBTAINEDFROMTHEMTTANALYSISANDICPMSANALYSISWASSUBJECTEDTOSTATISTICALANALYSISONEWAYANOVAWASUSEDTOCALCULATETHEPVALUEMULTIPLERANGETESTSBYTUKEY–HSDPROCEDUREWEREEMPLOYEDTOIDENTIFYTHESIGNIFICANTGROUPSAT5LEVELRESULTSELEMENTALRELEASEELEMENTALRELEASEWASSEENINALLTHREECASTINGALLOYGROUPSELEMENTSRELEASEDWERENI,CR,CO,CUANDMOTABLES4,AND5SUMMARIZETHEELEMENTALRELEASEBYDIFFERENTCASTEDALLOYSINTHREECASTINGPROCEDURESHINICKELCBHADTHEHIGHESTAMOUNTOFELEMENTALRELEASEAMONGTHETHREECASTINGALLOYSFOLLOWEDBYCERAMETANDWIRON99ELEMENTALRELEASESIGNIFICANTLYINCREASEDWITHTHEPERCENTAGEOFRECASTMATERIALUSEDINFORALLELEMENTSANALYZEDINTHESTUDYWITHP\005GRAPH1CYTOTOXICITYRESULTSTHEPERCENTAGEOFVIABLECELLSINTHREEGROUPSISTABULATEDINTABLE6MEANPERCENTAGESOFCELLACTIVITYOFTHREEBASEMETALALLOYSFORTHREECASTINGCONDITIONSWEREANALYZEDANDCYTOTOXICITYWASOBSERVEDTOBEMOREINHINICKELCB,FOLLOWEDBYCERAMETANDWIRON99RESULTSSHOWEDTHAT100RECASTALLOYSHADMORECYTOTOXICITYTHAN50PUREAND50RECASTEDAND50PUREAND50RECASTEDALLOYHADMORECYTOTOXICITYTHANPUREALLOYGRAPH2DISCUSSIONMETALSHAVEBEENANINTEGRALPARTOFDENTALRESTORATIVEPROCEDURESDENTALCASTINGALLOYSWHICHRESTORETHEFORMANDFUNCTIONOFMISSINGTOOTHSTRUCTURESHOULDBEBIOLOGICALLYINERTRELEASEOFMETALLICELEMENTSFROMDENTALCASTINGALLOYSISAPOTENTIALHEALTHPROBLEM2,8METALIONSLEACHEDFROMCASTINGALLOYSAREKNOWNTOCAUSETOXICINFLAMMATORY,ALLERGICORMUTAGENICREACTIONS9,10THESERELEASEDELEMENTSHAVEBEENDETECTEDBYINVITROANDINVIVOSTUDIESANIMPORTANTCONSEQUENCEOFELEMENTRELEASEISITSCYTOTOXICEFFECTONADJACENTTISSUEANDTHEAMOUNTOFTISSUEREACTIONDEPENDSUPONELEMENTRELEASED5–7,11,12FIG5DULBECCO’SMODIFIEDEAGLEM

注意事项

本文(外文翻译--关于重铸合金镍铬牙科铸造合金中元素的释放和细胞毒性的体外分析 英文版【优秀】.pdf)为本站会员(英文资料库)主动上传,人人文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对上载内容本身不做任何修改或编辑。 若此文所含内容侵犯了您的版权或隐私,请立即通知人人文库网(发送邮件至[email protected]或直接QQ联系客服),我们立即给予删除!

温馨提示:如果因为网速或其他原因下载失败请重新下载,重复下载不扣分。

关于我们 - 网站声明 - 网站地图 - 资源地图 - 友情链接 - 网站客服客服 - 联系我们

网站客服QQ:2846424093    人人文库上传用户QQ群:460291265   

[email protected] 2016-2018  renrendoc.com 网站版权所有   南天在线技术支持

经营许可证编号:苏ICP备12009002号-5