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Antibiotics and Resistance Genes in Biosolids,Mary Stromberger Soil Microbiology Department of Soil and Crop Sciences,What are Biosolids?,Organic, treated solids and semi-solids recovered during wastewater treatment Treated to reduce pathogen and heavy metal loads Suitable for soil amendment Source of organic matter, plant nutrients, but also heavy metals and salts,Recycling of Biosolids via land application: Emerging concerns,Dioxin? Endocrine disruptors? Nonylphenols Flame retardants,Denver Post 10/5/2004,Pharmaceuticals? Prescription drugs Antibiotics,Therapeutic agents as emerging contaminants in biosolids: persistence and impact on soil microbial communities,2-year project funded by EPA Region 8 Objectives: Screen biosolids collected from different wastewater treatment facilities for kinds and amounts of antibiotics present Determine the persistence of selected antibiotics, applied alone or along with biosolids, in soils under aerobic conditions Determine the effects of selected antibiotics on microbial activity and microbial antibiotic resistance patterns in soil during aerobic incubation Determine the long-term effects of biosolids, land applied to field soils, on antibiotic resistance patterns of soil microbial communities.,erythromycin,tetracycline,Concentration of macrolides in biosolids (g kg-1),Concentration of sulfonamides in biosolids (g kg-1),Concentration of tetracyclines in biosolids (g kg-1),Are there antibiotic-resistant bacteria in biosolids?,Oxytetracycline-resistant bacteria in Anaerobic A biosolids,Oxytetracycline-resistant bacteria in Aerobic A biosolids,Are the resistance genes to OTC carried on plasmids?,Horizontal gene transfer,Plasmids from OTC-resistant Aerobic A bacterial isolates,3,8,23,27,30,C,ML,Continuing research . . .,Laboratory study: determine how additions of OTC-contaminated biosolids, OTC, or OTC-resistant bacteria to soil affect indigenous soil microbial activities and antibiotic resistance patterns Field study: determine the antibiotic-resistance patter
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