补益中药微量元素的比较研究.pdf

第G21G22卷G21第G22期G21G21G21G21G21G21G21G21G21G21G21G21光谱学与光谱分析G23G24G25G26G21G22G21G27G24G26G22G21G28G28G29G2AG2EG2EG2CG29G2AG2EG2DG21G2FG2FG22年G22月G21G21G21G21G21G21G21G21G21G21G21G21G30G28G31G32G33G34G24G35G32G24G28G36G37G38G39G30G28G31G32G33G34G37G25G3AG38G37G25G36G35G3BG35G3CG3DG25G36G21G21G2FG2FG22G21补益中药微量元素的比较研究范文秀河南科技学院化工系G21河南新乡G21G2AG2DG2EG2FG2FG2E摘G21要G21采用火焰原子吸收法对G21G2F种补益中药中G62G38G21G5CG3DG21G3FG38和G66G31等微量元素的含量进行了分析测定G22该方法的加标回收率为G2BG44G26G3EG42G23G29G2FG2AG26G2EG42G21G60G30G4F值小于G21G26G2FG42G21具有良好的准确度和精密度G22研究结果表明G26补益中药中的G62G38G21G5CG3DG21G3FG38和G66G31含量丰富G21补血药中的G66G31含量最高G21补气药中G62G38G21G3FG38和G66G31含量较高G21补阳药中G62G38G21G3FG38含量较高G21补阴药中G66G31含量较低G22此测定结果可为探讨补益中药中微量元素的含量与其药效的相关性提供科学依据G22关键词G21补益中药G23微量元素G23火焰原子吸收法中图分类号G21G43G44G2DG22G26G2EG21G21文献标识码G21G3AG21G21G21文章编号G21G29G2FG2FG2FG2CG2FG2DG2BG2EG22G21G2FG2FG22G23G2FG22G2CG29G2AG2EG2EG2CG2FG2EG21收稿日期G21G21G2FG2FG44G2CG2FG2DG2CG21G3EG24修订日期G21G21G2FG2FG44G2CG2FG2BG2CG2FG21G21基金项目G21河南省科技攻关项目G24G2FG2EG21G2AG2FG22G2FG29G2FG2FG21G2FG2AG21G2AG2FG22G2FG2FG2DG2FG25资助G21作者简介G21范文秀G21G29G2BG44G2E年生G21河南科技学院化工系教授G21G21G31G2CG45G37G3BG25G26G51G56G54G3BG3DG21G47G3BG35G33G26G31G39G3DG26G32G38引G21言G21G21近年来G21人们对中草药中的无机成分尤其是微量元素的研究产生了浓厚的兴趣G27G29G2CG2AG28G21一些必需的微量金属离子在许多生物大分子G21如核酸G29蛋白质G29酶和激素中具有特别重要的功能G27G2DG28G21它们是肌体细胞正常代谢所必需的催化剂G22这些元素的缺乏或过多均会引起人体疾患G22现代研究证明G21特定状态下的微量元素是维持健康和防病治病的必要条件和物质基础G27G44G28G22补益中药在中药中占有重要的地位G21根据补益中药的功能不同G21补益中药可分为补气药G29补阳药G29补血药和补阴药四类G27G22G28G22补益中药具有免疫G29预防G29治疗G29保健等多种功效G22本文采用火焰原子吸收法对G21G2F种补益中药中G66G31G21G62G38G21G5CG3D和G3FG38等微量元素进行了测定G21以期从元素的角度探讨补益中药的多种药用作用G21为研究补益中药中微量元素与疗效的内在联系以及中草药真伪的鉴定提供科学依据G22G29G21实验部分G25G24G25G21仪器和工作条件G49G66G59G2CG29G29G2F型原子吸收分光光度计G24北京瑞利分析仪器厂G25G21附计算机和软件处理系统G22仪器工作条件见表G29G22G25G24G35G21标准溶液G62G38G21G5CG3DG21G3FG38G21G66G31的标准储备液均由相应的光谱纯金属配制G21其标准储备液的浓度均为G29G45G46G2CG45G5BG4DG29G21使用时再逐级稀释成标准使用液G22G25G24G38G21样品处理G29G26G2EG26G29G21样品来源补益中药样品包括G26补气药党参G29五味子G29黄芪G29山药G29甘草G23补阳药淫羊霍G29肉苁蓉G29菟丝子G29杜仲G29仙茅G23补血药当归G29枸杞子G29熟地G29桑椹G29龙眼肉G23补阴药女贞子G29玉竹G29石斛G29黄精G29麦冬G22以上药品均购于河南省百泉药材交易市场G22G49G2BG37G33G29G25G21G50G3DG29G2EG2BG2CG22G31G23G27G2FG31G2DG22G2CG22G2FG31G39G21G21元素G62G38G5CG3DG3FG38G66G31波长G2AG38G45G21G29G2EG26G2BG2EG21G2AG26G3EG21G22G2BG26G2DG21G2AG3EG26G2E灯电流G2AG45G3AG2EG26G2FG2DG26G2FG2EG26G2FG2AG26G2F狭缝G2AG38G45G2FG26G21G2FG26G21G2FG26G21G2FG26G21燃烧器高度G2AG45G45G21G26G2FG21G26G2FG21G26G2FG21G26G2F乙炔流量G2AG24G5BG2CG45G3BG38G4DG29G25G2EG26G2FG2EG26G2FG2EG26G2FG2EG26G2F空气流量G2AG24G5BG2CG45G3BG38G4DG29G25G29G2FG26G2FG29G2FG26G2FG29G2FG26G2FG29G2FG26G2FG49G2BG37G33G29G35G21G26G2CG2BG31G2DG2BG2EG2DG39G2FG33G34G2CG22G2FG31G22G21G23G2FG2AG4FG5EG25G23元素G62G38G5CG3DG3FG38G66G31G30G4CG4FG2FG2FG26G2FG2FG2FG26G2FG2FG2FG26G2FG2FG2FG26G2FG2FG30G4CG4FG29G2FG26G29G2FG2FG26G2DG2FG2FG26G29G2FG29G26G2FG2FG30G4CG4FG21G2FG26G21G2FG29G26G2FG2FG2FG26G21G2FG21G26G2FG2FG30G4CG4FG2EG2FG26G2EG2FG29G26G2DG2FG2FG26G2EG2FG2EG26G2FG2FG30G4CG4FG2AG2FG26G2AG2FG21G26G2FG2FG2FG26G2AG2FG2AG26G2FG2FG30G4CG4FG2DG2FG26G2DG2FG21G26G2DG2FG2FG26G2DG2FG2DG26G2FG2FG29G26G2EG26G21G21样品处理将中药样品用蒸馏水洗净G21在G44G2FG68烘箱中低温烘干G21用多功能粉碎机进行粉碎G22准确称取中药样品各G29G26G2FG2FG2FG2FG46G21在电炉上加热炭化G21在G2DG2FG2FG68的马弗炉里加热G3EG23G29G2FG47G21待样品全部灰化后G21冷却至室温G21加G29G6BG29G53G27G43G2E溶液G21G45G5BG21微微加热使其溶解后定容至G2DG2FG45G5B容量瓶中待用G22按同样方法制备空白样品G22G25G24G43G21测定G29G26G2AG26G29G21标准系列分别吸取适量的标准使用液G21用蒸馏水稀释成表G21的系列标准溶液G22G29G26G2AG26G21G21样品测定按表G29所列仪器的工作条件G21用火焰原子吸收法测定样品溶液中G62G38G21G5CG3DG21G3FG38和G66G31的含量G22根据样品含量及各元素测定灵敏度的不同G21各元素测定时分别稀释适当的倍数G22G21G21结果与讨论G35G24G25G21方法的准确度和精密度为考察方法的可靠性G21用补气药进行了加标回收试验G21各元素的回收率在G2BG44G26G3EG42G23G29G2FG2AG26G2EG42之间G21相对标准偏差G24G60G30G4FG25值小于G21G26G2FG42G21结果表明该方法具有良好的准确度和精密度G22G35G24G35G21样品的测定结果按上述方法测定了G21G2F种补益中药G62G38G29G5CG3DG29G3FG38和G66G31四种微量元素的含量G21测定结果见表G2EG22G49G2BG37G33G29G38G21G44G2FG31G27G29G31G2CG2EG2BG2CG22G2FG31G2FG30G2CG2EG2BG27G29G29G33G29G2AG29G31G2CG39G22G31G39G2BG2AG3DG33G29G39G22G21G23G2FG23G5EG25G24G2BG32G29G2EG2BG23G29G23G22G2AG64G46G23类别名称G62G38G5CG3DG3FG38G66G31补气党参G21G44G26G2DG2DG2DG26G29G22G2EG29G26G21G29G2AG21G3EG26G22G2B五味子G21G2DG26G2FG29G2FG26G2BG3EG29G3EG26G2DG44G3EG2EG44G26G2FG2F黄芪G21G22G26G29G22G2DG26G2FG2EG21G21G26G44G2EG44G2DG2BG26G2EG44山药G21G44G26G3EG2BG44G26G3EG2BG44G26G2AG2DG2BG2EG26G3EG2D甘草G2AG2DG26G2DG44G29G2EG26G2AG2DG29G2AG26G2DG2FG44G2FG22G26G29G21补阳淫羊霍G2EG21G26G2AG2AG29G2FG26G2DG2DG3EG21G26G2AG44G29G44G2FG26G2BG44肉苁蓉G29G3EG26G29G2EG2DG26G2DG2FG2EG29G26G2FG21G2AG44G29G26G44G21菟丝子G21G2DG26G21G21G2DG26G2EG22G29G2FG2FG26G22G2FG21G3EG21G26G22G2F杜仲G29G3EG26G29G3EG2EG26G2AG2FG29G2AG2BG26G2EG21G2EG2AG44G26G3EG22仙茅G21G21G26G2AG2FG3EG26G44G21G29G2AG2FG26G3EG29G21G2AG2EG26G2DG2B补血当归G21G2FG26G2DG2BG22G26G44G2EG2EG21G26G2DG2DG2DG2FG2BG26G2BG2B枸杞子G21G2AG26G21G21G29G2EG26G29G3EG2EG2EG26G2BG2DG2AG29G22G26G29G22熟地G29G22G26G3EG2BG21G26G2DG44G2EG2FG26G2BG2AG2BG3EG22G26G2AG2D桑椹G21G29G26G2BG29G29G44G26G2BG2FG2AG21G26G2DG3EG2BG2BG3EG26G2DG29龙眼肉G2EG2EG26G2FG21G29G2EG26G2FG2EG2EG44G26G2DG2EG21G44G2DG26G2FG2F补阴女贞子G21G2DG26G2FG2FG29G2FG26G21G2FG2EG29G26G2FG2FG2AG22G44G26G2FG2F玉竹G2EG21G26G2AG29G29G2DG26G3EG29G2EG22G26G44G21G21G2FG22G26G2EG2D石斛G29G2FG26G2EG29G2EG26G2EG2DG29G2EG26G44G2AG22G29G26G2DG44黄精G2EG2AG26G2DG2DG2BG26G2DG44G2EG44G26G29G2DG2EG2EG44G26G2DG29麦冬G2DG26G2DG22G2EG26G2EG3EG2FG26G2AG2DG29G29G2EG26G2BG2AG35G24G38G21元素含量及特征从测定结果可以看出补益中药中G62G38G21G5CG3DG21G3FG38和G66G31四种微量元素的含量都非常丰富G21它们被称为与人体生命攸关的元素G27G3EG28G21这四种元素对人类的生长发育G29造血功能G29免疫功能有着重要的作用G22药用植物生长不仅需要必需的营养元素G21而且还能选择地吸收和富集某些人体所需的有益的微量元素G21这些元素是临床中起防病治病作用的物质基础之一G27G2BG28G22从表G2E可以看出G21补气G29补阳药中G62G38含量显著高于补血G29补阴药G22这一测定结果显示G21补气G29补阳药的某些功用与其所含的G62G38有关G22国内有关研究证明G27G29G2FG28G21G62G38是人体的G30生命之花G31G21男子精子活力与白蛋白和G62G38结合率有关G21进一步揭开了G62G38与G30肾藏精G21主生骨G31等朴素的我国医学理论的实质与精华G21说明G62G38是G30归肾经G31的物质基础G22大部分补气G29补血药G66G31含量都比较高G21G66G31是人体造血所必须的主要微量元素G22G66G31参与血红蛋白G29肌红蛋白G29细胞色素G29细胞色素氧化酶G29过氧化氢酶G29过氧化酶的合成G21并与乙酰辅酶G3AG29唬珀酸脱氢酶G29细胞色素还原酶的活性密切相关G22三羧酸循环中有一半以上的酶与因子含有G66G31或必须有G66G31存在才能发挥生化作用G29完成生理功能G22血红蛋白具有运输氧的功能G21而肌红蛋白起固定和储存氧的作用G27G29G29G28G22本实验所测定的G29G2F种补血G29补气药中大部分G66G31含量很高G21这与它们的补血和促进造血功能相吻合G22而在某些补气G29补血药中G62G38和G66G31含量都较高G21因而具有补气和补血的双重功效G21如黄芪G29当归号称气中血药G21补气又能补血G21主治劳倦内伤G21或失血后衰弱而血虚气虚G21气不摄血的病症G21气血兼补G21疗效卓越G22G5CG3D参与造血过程G21参与形成含G5CG3D蛋白质G21是细胞色素氧化酶G29血浆G5CG3D蓝蛋白G29超氧化物歧化酶等许多酶的组成成分G22血浆G5CG3D蓝蛋白参与G66G31的运输和代谢G21从而有利于人体内储存G66G31的动用和吸收G27G29G21G28G22补血G29补气药中补血生血的作用与其含G5CG3D有一定的内在联系G22G3FG38参与人体内氧化还原过程G29组织呼吸G29骨的形成G21影响生长发育G29血液的形成和内分泌功能G27G29G2EG28G22G3FG38具有保护肌体细胞和体液免疫系统的正常功能G21黄芪G29党参等具有增强免疫功能的作用G21可能与其含有较高的G3FG38有关G22由测定结果可以看出G21补血药中G66G31含量最高G21补气药中G62G38G21G3FG38和G66G31含量较高G21补阳药中G62G38和G3FG38含量较高G21补阴药中G66G31含量较少G22中药的功效是通过微量元素的G30归经G31来完成的G21不但中药中微量元素的涵量与其功效有密切关系G21而且还与各种微量元素的比例有关系G27G29G2AG28G22中药配伍所起到的疗效G21是通过配伍来改变方剂中的微量元素含量的比例G21以达到更好的疗效G27G29G2DG28G22因此研究补益中药微量元素的含量及其特点对进一步研究药物的功效G21开发新药具有重要的意义G22G2AG2EG2AG29光谱学与光谱分析G21G21G21G21G21G21G21G21G21G21G21G21G21G21G21G21G21G21G21第G21G22卷参考文献G27G29G28G21G66G5AG62G47G3BG2CG47G24G38G46G21G59G61G5DG3FG3BG38G46G2CG36G24G38G46G21G62G53G3AG27G55G62G47G3BG2CG45G3BG38G46G21G31G33G37G25G24付志红G21谢明勇G21章志明G21等G25G48G30G28G31G32G33G34G24G35G32G24G28G36G37G38G39G30G28G31G32G33G34G37G25G3AG38G37G25G36G35G3BG35G24光谱学与光谱分析G25G21G21G2FG2FG2AG21G21G2AG24G44G25G26G22G2EG22G48G27G21G28G21G5CG53G5DG27G5BG37G38G2CG4EG3DG21G62G53G5DG27G55G5BG3BG37G38G2CG36G3BG21G62G53G3AG43G4FG3BG2CG35G47G3DG38G21G31G33G37G25G24陈兰菊G21郑连义G21赵地顺G21等G25G48G30G28G31G32G33G34G24G35G32G24G28G36G37G38G39G30G28G31G32G33G34G37G25G3AG38G37G25G36G35G3BG35G24光谱学与光谱分析G25G21G21G2FG2FG2AG21G21G2AG24G3EG25G26G29G2FG29G2EG48G27G2EG28G21G55G43G27G55G3CG3BG2CG36G3DG21G59G5AG4CG3BG37G38G2CG36G37G38G46G21G57G5AG50G31G38G46G21G31G33G37G25G24贡济宇G21许天阳G21于G21澎G21等G25G48G30G33G3DG39G36G24G51G4CG34G37G32G31G5DG25G31G45G31G38G33G37G38G39G53G31G37G25G33G47G24微量元素与健康研究G25G21G21G2FG2FG21G21G29G2BG24G2AG25G26G44G3EG48G27G2AG28G21G66G3AG27G49G31G38G2CG54G3BG3DG21G5BG61G59G3BG38G2CG64G47G31G38G46G21G3CG61G27G55G60G3DG3BG2CG4EG3DG38G24范文秀G21李新峥G21荆瑞俊G25G48G30G28G31G32G33G34G24G35G32G24G28G36G37G38G39G30G28G31G32G33G34G37G25G3AG38G37G25G36G35G3BG35G24光谱学与光谱分析G25G21G21G2FG2FG44G21G21G44G24G2EG25G26G2DG44G22G48G27G2DG28G21G57G3AG27G30G47G3BG2CG45G3BG38G46G21G5BG61G62G31G38G46G2CG54G3BG21G59G61G43G27G55G5BG3BG2CG28G3BG38G46G24颜世名G21李增喜G21熊丽萍G25G48G55G3DG37G38G46G39G24G38G46G4CG34G37G32G31G5DG25G31G45G31G38G33G35G30G32G3BG31G38G32G31G24广东微量元素科学G25G21G21G2FG2FG21G21G2BG24G2BG25G26G29G48G27G44G28G21G66G3AG27G49G31G38G2CG54G3BG3DG21G57G3AG27G55G30G3DG2CG25G37G38G21G3CG61G27G60G3DG3BG2CG4EG3DG38G21G31G33G37G25G24范文秀G21杨素兰G21荆瑞俊G21等G25G48G55G3DG37G38G46G39G24G38G46G4CG34G37G32G31G5DG25G31G45G31G38G33G35G30G32G3BG31G38G32G31G24广东微量元素科学G25G21G21G2FG2FG2DG21G29G21G24G22G25G26G2DG21G48G27G22G28G21G4CG47G31G43G51G51G3BG32G3BG37G25G5CG24G45G45G3BG33G33G31G31G24G51G53G36G46G3BG38G31G4FG31G28G37G34G33G45G31G38G33G24G51G33G47G31G50G31G24G28G25G31G32G35G60G31G28G3DG40G25G3BG32G24G51G5CG47G3BG38G37G24中华人民共和国卫生部药典委员会编G25G48G50G47G37G34G45G37G32G24G28G24G31G3BG37G24G51G33G47G31G50G31G24G28G25G31G32G35G60G31G28G3DG40G25G3BG32G24G51G5CG47G3BG38G37G24G50G37G34G33G2BG2CG21G2FG2FG2FG25G24中华人民共和国药典一部G21G21G2FG2FG2F年G25G48G41G31G3BG4EG3BG38G46G26G5CG47G31G45G3BG32G37G25G61G38G39G3DG35G33G34G37G25G50G34G31G35G35G24北京G26化学工业出版社G25G21G21G2FG2FG2FG48G2EG2BG2DG48G27G3EG28G21G53G5AG3AG27G55G3CG3BG37G38G21G27G61G3CG3BG38G46G2CG40G3BG38G24黄G21坚G21倪敬斌G25G48G55G3DG37G38G46G39G24G38G46G4CG34G37G32G31G5DG25G31G45G31G38G33G35G30G32G3BG31G38G32G31G24广东微量元素科学G25G21G29G2BG2BG22G21G2AG24G2BG25G26G29G2EG48G27G2BG28G21G5CG53G5DG27G55G5CG3DG38G2CG46G3DG3BG21G5BG61G4FG37G38G2CG33G3BG38G46G21G5BG61G5AG59G3BG38G46G2CG47G37G3BG21G31G33G37G25G24程存归G21李丹婷G21刘幸海G21等G25G48G30G28G31G32G33G34G24G35G32G24G28G36G37G38G39G30G28G31G32G33G34G37G25G3AG38G37G25G36G35G3BG35G24光谱学与光谱分析G25G21G21G2FG2FG44G21G21G44G24G29G25G26G29G2DG44G48G27G29G2FG28G21G62G53G3AG43G66G31G38G46G2CG64G31G21G30G53G5DG27G55G37G38G46G2CG64G47G31G21G3CG61G3AG27G55G57G3BG38G46G2CG64G3BG24赵凤泽G21沈刚哲G21姜英子G25G48G55G3DG37G38G46G39G24G38G46G4CG34G37G32G31G5DG25G31G45G31G38G33G35G30G32G3BG31G38G32G31G24广东微量元素科学G25G21G21G2FG2FG21G21G2BG24G2EG25G26G21G2AG48G27G29G29G28G21G62G53G3AG27G55G59G3BG37G24G2CG56G31G3BG21G5BG61G53G3DG37G24张晓薇G21李G21华G25G48G3CG24G3DG34G38G37G25G24G51G30G47G37G38G54G3BG5CG24G25G25G31G46G31G24G51G4CG34G37G39G3BG33G3BG24G38G5CG47G3BG38G31G35G31G3FG31G39G3BG32G3BG38G31G24山西中医学院学报G25G21G21G2FG2FG29G21G21G24G21G25G26G29G22G48G27G29G21G28G21G5BG61G5AG57G37G38G2CG45G3BG38G46G24刘彦明G25G48G30G28G31G32G33G34G24G35G32G24G28G36G37G38G39G30G28G31G32G33G34G37G25G3AG38G37G25G36G35G3BG35G24光谱学与光谱分析G25G21G21G2FG2FG2FG21G21G2FG24G2EG25G26G2EG22G2EG48G27G29G2EG28G21G5BG61G3AG27G55G30G47G3DG2CG54G3DG37G38G21G30G5AG27G53G37G38G2CG56G31G38G24梁淑轩G21孙汉文G25G48G30G28G31G32G33G34G24G35G32G24G28G36G37G38G39G30G28G31G32G33G34G37G25G3AG38G37G25G36G35G3BG35G24光谱学与光谱分析G25G21G21G2FG2FG21G21G21G21G24G2DG25G26G3EG2AG22G48G27G29G2AG28G21G5BG5DG57G3DG37G38G24乐G21原G25G48G5CG47G3BG38G31G35G31G3CG24G3DG34G38G37G25G24G51G33G47G31G50G34G37G32G33G3BG32G37G25G5CG47G3BG38G31G35G31G56G3BG33G47G3FG24G39G31G34G38G3FG31G39G3BG32G3BG38G31G24中华实用中西医杂志G25G21G21G2FG2FG2EG21G2EG24G29G44G25G26G29G3EG21G2DG48G27G29G2DG28G21G3FG3AG63G3BG37G38G46G21G5BG61G5AG3CG3DG38G21G30G5AG59G3BG38G46G2CG46G3DG37G38G46G24马G21强G21刘G21军G21苏星光G25G48G3CG24G3DG34G38G37G25G24G51G3CG3BG25G3BG38G5AG38G3BG5FG31G34G35G3BG33G36G24G30G32G3BG31G38G32G31G5DG39G3BG33G3BG24G38G25G24吉林大学学报G2C理学版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