流动注射时间扫描荧光分析法测定泛昔洛韦.doc

exem0100200300400500600700800200300400500/nm荧光强度F流动注射时间扫描荧光分析法测定泛昔洛韦马红燕*，高瑞，孙雪花延安大学化学与化工学院，分析化学研究所，716000，延安E-mail:M泛昔洛韦（Famciclovir）为无环核苷类抗病毒药，临床上应用广泛。测定方法主要有：高效液相色谱法1、紫外分光光度法2和毛细管电泳法3等，利用荧光分析法直接测定泛昔洛韦的研究未见有文献报道。实验发现，泛昔洛韦具有强的内源性荧光，结合流动注射进样技术，借助时间扫描荧光方式，提出了流动注射时间扫描荧光分析法直接测定泛昔洛韦的新方法。体系最大激发和发射波长分别为309.0nm和380.0nm。泛昔洛韦浓度在2.810-7g/mL4.010-5g/mL范围内与荧光强度呈良好的线性关系，相关系数为0.9997。方法检出限为5.810-9g/mL，相对标准偏差为0.4%（n=11,c=4.010-7g/mL），进样量0.35mL，进样频率为100/h。方法用于药物制剂及尿样中泛昔洛韦的直接测定，结果满意。Fig.1Schematicdiagramofflow-injectionfluorometryforthedeterminationoffamciclovirP1,P2-Peristalticpump；3-Injectingvalve；4-Pipeflow；Sample-Solutionoffamciclovir；C-Carriersolution：Britton-RobinsonbuffersolutionFig.2Fluorescencespectrumoffamciclovir关键词:泛昔洛韦；流动注射分析；时间扫描；荧光光度法。参考文献：1F.Schenkel,S.Rudaz,Y.Daali,M.KondoOestreiche,J.-L.VeutheyandP.Dayer.JournalofChromatographyB,2005，826(1-2)，1-72张锦华西药学杂志，2006，21(3)，303-3033金瓯，黄宗玉中国生化药物杂志，2001，22(4)，189-190ATimeScanningFluorophotometricMethodfortheDeterminationofFamciclovirbyFlowInjectionAnalysisHong-YanMA*,RuiGAO,Xue-HuaSUNCollegeofChemistryandChemicalEngineering,InstituteofAnalyticalChemistry,YananUniversity,716000,YananThefluorescencecharacteristicsofthefamciclovirhavebeenstudied.Theexperimentsindicatethatfamciclovirhasendogenousfluorophoreswhichcanemitstrongfluorescencesignals.Basedonit,combinedwithflowinjectionanalysisandtimescanningtechnology,anewflow-injectionfluorescencemethodforon-linedeterminationoffamciclovirisestablished.Theexcitationandemissionwavelengthsare309.0and380.0nm,respectively.Thelinearrangeis2.810-7g/mL4.010-5g/mL,thedetectionlimitis5.810-9g/mL,therelativestandardderivationis0.4%(n=11,c=4.010-7g/mL),thesamplingamountis0.35mL,thefrequencyofsampleintroductionis100samples/h.Themethodhasbeen