HPLC 测定抗癫痫中药! 中四种西药成分血药浓度.pdf

,Y3,vembD0hZAr,bnullDpnull；,A?r5b,AT=7|!b.,|E7L.SbLSL,a|a|,SL|,$bK,SL|K,7Y5aaBa+aaPa0aIaCava160F,ma|Ya77L#=erbZv,7,|Y；aCav0,.|0#rs7T.SBLl,2003,9(12):726HPLCFu0!0s0i*1,o2,V1(1.?gD,?300060；2.?g8D8,?300074)K1:rAMEHFu0!0s&1a5a&0i,5ara0bZE:rAME,SymmtryC18,A-Y(64#36)M,_o214nmbY,bAsbT:&1a5a&sX,sY5.050.0a1.012.0a5.025.00.102.00mg/LS=L1z,=W5%,(Mlq98.65%102.64%b:ZEeLayaa,a50S,50ara4b1oMHPLC,Fu0,0i,&1,5,&,ms|:R97DSM:AcI|:1006null5687(2010)04null0032null03Determinationofblooddruglevelsoffourchemicalcomponentsofanti-epilepticTraditionalChineseMedicinesbyHPLCYanWeiwei1,LiHuifen2,RenHuiqin1(1.TianjinHuanhuHospita,lTianjin300060；2.TianjinChildrensHospita,lTianjin300074)ABSTRACTObjective:Tosimultaneouslydeterminetheblooddruglevelsoffourchemicalcomponents,phenobarbita,lcarbamaznullepine,diphenylhydantoinanddiazepam,ofanti-epileptictraditionalChinesemedicinepreparationsbyhigh-performanceliquidchromatography(HPLC),soastorealizerationalutilizationofthemedicine.Methods:SymmtryC18columnwasemployedasstanulltionaryphaseandthesolutionofphosphatebufferandacetonitrile(64#36)asmobilephase.Thewasdetectedatthewavelengthof214nm.Theproteininbloodwasprecipitatedwithacetonitrile.Results:Phenobarbita,lcarbamazepine,diphenylhydantoinanddiazepamwerewellseparatedandshowedideallinearcorrelationintherangeof5.050.0,1.012.0,5.025.0and0.102.00mg/Lrespectively.Thedifferencesofprecisionwithinadayandbetweendayswerelowerthan5%.Theaveragerelativerenull32?0TianjinPharmacy2010M224*l:2010null03null11coverywasbetween98.65%and102.64%.Conclusion:Thisapproachisconvenient,rapid,sensitive,preciseandaccurate,benullingsuitablefortherapeuticdrugmonitoring(TDM)andguidingclinicalrationaluseofthedrug.KEYWORDSHPLC,anti-epileptic,TraditionalChineseMedicine,blooddruglevels-,Su600,M?h65701buh1|0,#E0Tl7i0b7,uBt04c0s,t3Q,O5D3F0LbyN,B0,_AB00i,ZL5Bb-K_ZErAMEbB,50LiY!BrAMN,0iH,ZEWMnHan,Yh=h0,iO9FTbN,yBHq/,&1a5a&0iZE,V54eLayaTb1Nk01null1NSWatersrAMN,!600E,486VMo_,pc-800T_b1null20k4&1(171222-200605)a5(100142-199503)a&(100210-200401)(171225-200302)v(S03_),5-5-Y1(SSigma),YaJ?B,k4sBk4b2ZET2null1Hq:SymmtryC18(4.6mm%150mm,5nullm)；M:A(pH6.9)Y8164#36；:1.2ml/min,_o:214nmb:i,:10nulllb2null2b)|Sb50null,lF80nulllc=S5-5-Y110mg/LYA,10000r/min5min,|bA10nulllb=SE9Scb2null3A2.3.1vAGQ|&1a5a&v50a25a5010mg,J?sY1.0a0.5a1.00.2g/LvA,4&ib2.3.2v|bbsYF&1a5a&vA,&15.0a10.0a20.0a30.0a40.050.0mg/L；51.0a2.0a4.0a8.012.0mg/L；&5.0a10.0a15.0a20.025.0mg/L；0.10a0.25a0.50a1.002.00mg/Livbb2null4kHq/,&1a5a&a=SsrTz,=HWsY2.98a4.82a5.68a17.634.21minbb=Y,nm1bHq/,Y0aaday.abaoba:a:a:s,(&1a5a&b1.&12.=S3.54.&5.m1b&1a5a&aHPLCm2null5SwL#_K|2.3.2!/A,v=S1:US(Y),iUS(X)LBbTb&1a5a&sY5.050.0a1.012.0a5.025.00.102.00mg/LiS=L1z,BZsY&1Y=0.04936X+0.009753,r=0.9997；5Y=0.08198X-0.0007091,r=0.9997；&Y=0.04086X-0.001901,r=0.9996；Y=0.07092X+0.0009930,r=0.9994bb&1a5a&K_isY0.1a0.1a0.20.05mg/Lb2.6lqk|&110.0a20.0a40.0mg/L,54.0a8.0a12.0mg/L,&5.0a10.0a20.0mg/L,0.50,1.00,1.50mg/L3ibS6z,p=；E,|bS6z,8d=,pWb|BbS,F&1a5a33?0TianjinPharmacy2010M224&vTlk,&1a5a&Mlq,nV1b=W(l5%,(Mlq98.65%102.64%,ZE1pbV1lqk0i(mg/L)=k(n=6)L(mg/L)RSD(%)Wk(n=6)L(mg/L)RSD(%)Mlq(n=3)L(mg/L)RSD(%)&110.010.060.181.7910.050.232.29101.871.311.2920.019.840.381.9219.880.482.4199.651.511.5240.040.120.521.3140.020.731.8299.540.460.4654.04.060.051.233.940.061.5298.651.821.828.08.300.131.567.950.101.2698.772.212.2312.012.760.141.1011.810.201.69101.111.881.86&5.05.040.112.275.010.122.40101.202.982.9510.010.030.151.4910.100.212.0899.972.762.7620.020.080.271.3519.860.452.27100.182.732.720.500.530.023.770.490.024.08102.644.124.011.001.010.021.981.010.032.97100.443.443.421.501.510.042.651.430.064.1999.173.323.342.7Fu0!0s0i_f2007M3(2009M2*=SgrFu0!u32,0(SFu0s,W(BFu0,0s0i,_0fnV2bV2Fu0!0s0i_T0K0i(mg/L)K0i(mg/L)&1234.541.85151.77.8&80.712.130.050.163)3null1uh*Snh,70%80%uhVYVa?BLFu0e1b-,Fu040sC1nb0!,n5W%0!8f,0H1In0!0MT,#0!V?c0sbc0sFu0!,A_0ibc0#ri,0,0S!bH3Fu0!E,S30c0s,502b3EIn0!0WV?CMT,FZ,Nu05,EA10b3null2HqH,MpHEs1o,pH0.1H,=Sa5&s,sb3null3=S5-5-Y1,M,d50,BX=S