地塞米松经鼓室给药对豚鼠耳蜗结构和功能的影响.pdf

T:200031Z,v90SD90S(yabZ)；vBD90S()YT:y,Emai:llhjent163nullcomnullLnulli09?Y刘宏建null董明敏null迟放鲁nullnullK1nullnullNi0o9?YbZEnull453VE$s3F,Fa3i0Fai0F,F15bP7Q(auditorybrainstemresponse,ABR)a(superoxidedismutase,SOD)a9FB(nitricoxide,NO)ca9V?Ni0H9?YbTnull3FABRoosd9il(F=0null5,P=0null75)；3FSODsd9il(F=2null45,P=0null96)；3FNOcsd9il(F=3null01,P=0null83)；3F9%M1sd9il(F=0null93,P=0null45)bi09/?Yb1oMnull；null；nullD；nullStructureandfunctioninfluenceofcochlearafterdexamethasoneintratympanicapplicationnullnullLIUHongnulljian*,DONGMingnullmin,CHIFangnulllunull*DepartmentofOtorhinolaryngology,EyeEarNoseandThoratHospital,FudanUniversity,Shanghai200031,ChinaCorrespondingauthor:LIUHongnulljian,Email:lhjent163nullcomAbstractnullObjectivenullTostudythechangesofthestructureandfunctionofcochlearafterdexamethasoneintratympanicapplicationnullMethodsnullFortynullfiveguineapigsweredividedintothreegroupsasnormalgroup,0null9%sodiumchlorideinjectiongroupand0null5%dexamethasonegroupnullByusingtheauditorybrainstemresponse(ABR),thesuperoxidedismutase(SOD)activity,thenitricoxide(NO)valueandcochlearsurfacepreparationtechnique,thechangesofstructureandfunctionofcochlearafterintratympanicDexamethasoneapplicationhasbeeninvestigatedinthisstudynullResultsnullTherewasnotsignificantdifferenceamongthreegroupsinABRthresholdsofwave(F=0null5,P=0null75)nullTherewasnotsignificantdifferenceamongthreegroupsinSODactivity(F=2null45,P=0null96)nullTherewasnotsignificantdifferenceamongthreegroupsinNOvalue(F=3null01,P=0null83)nullTherewasnotsignificantdifferenceamongthreegroupsinnecroticvaluesofhaircellsonthecochlearsurfacespecimensin15mmfrombasalturn(F=0null93,P=0null45)nullConclusionnullThereisnochangeofcochlearconstructionandfunctionafterintratympanicDexamethasoneapplicationnullKeywordsnullDexamethasone；nullGuineapigs；nullDeafness；nullLabyrinthnullnullM,l?DaDha1&f=9h1Tb?&H,VIn0bVnullio=9,yNA1i0bLYVyi0,Ni0H9?YbZEnullnullBa1Nk41null1N:CadwellQuantum84P7Q(auditorybrainstemresponse,ABR)N(Cadwel,lS),OlympusA(Olympus,),YYO027null90Q(2YkNK),TGLnull16G(ZN!K),721s；9(ZsN9),UVnull200l,Vns；N(,),Q(?),JA12030?(Z?N),FZQnull2s(aYKnull440null90hS2005M6406nullChinJOtorhinolaryngolHeadNeckSurg,June2005,Vol40,No.6)b2null1k4:)A,=Y(ethylenediaminetetraaceticaciddisodium,EDTA)a50%=aoaB(nitricoxide,NO)k4a(superoxidedismutase,SOD)k4b=aLsF9Q2B45,8200300g,IK,|I|,3VZE|s3F:F:15,o0null3ml/kg8J,kABRyh,|5sY9FSODaNOc9；3F:15,o0null3ml/kg8J,kABR,Oi100nullL3；YJ,?,Oi100nullL31Q,7d；7d,o0null3ml/kg8J,ARRkyh,|5sY9FSODaNOc9；F:15,o0null3ml/kg8J,kABR,Oi100nullL(0null5mg)；YJ,?,O100nullL1Q,7d；7d,o0null3ml/kg8J,ABRkyh,|5sY9FSODaNOc9baLZE1nulli0y:J%O,|99,Bm9,BmO|100nullLA898,9_bb2.ABRk:|10%o(200300mg/kg)8J7i,CadwellQuantum84?dkbkW38bABRoooKv,ABRo,oTABRQS,2)5dB)4oM,ohb3.9FSOD#NOc:9FSODk:ZEvk4,bESOD,Tx6(U/ml)VUb&Jyh),b3|9,0?,b/0null9%32%F,A,s,3740min,FA4,10min721s；9,o550nm)15；b9FNOc:ZEvk4b&Jyh),b3|9,0?,b/310%F,A,s,3760min,FA30s,i10min,/1000r/min,5min,|b,FA4,10min,UVnull200l,Vns；N,o550nm)1；b4null9:&Jh|9,b0ob:TA,VU9TA9%W#,|9937ob:TA1h,!,10%J%24h,!,10%EDTA710d,!,PBS(pH7null4)A,A/CXT,/,s,%ZC,lUM,|V|/,C-V,|_,bj99%,j,MA/4%sb9O920mm,%?5,99400P,U9%b912,S_15mmSbL%9N15mmSbad9)SPSSl0null0q),yZ(F)s,TFWs,(1q_bP0null05sd9il_SbTnullnullBaABRTFABR(2)T(nullx!s):F(17null00!3null59)dB；39-(18null00!4null36)dB,9(18null00!3null49)dB；null441null90hS2005M6406nullChinJOtorhinolaryngolHeadNeckSurg,June2005,Vol40,No.6m1nullF=%,L8Rbob:300nullnullm2null3F=%,L8Rbob:300nullnullm3nullFAU=%,L8Rbob:3009-(17null00!3null19)dB,9(17null00!2null65)dBbFABRZs,FWsd9il(F=0null5,P=0null75)b=a9FSOD#NOc9FSOD#NOcnV1,FSODsd9il(F=2null45,P=0null96)；FNOcsd9il(F=3null01,P=0null83)bV1null9FSOD#NOcT(nullx!s)FYkHWSOD(U/ml)NO(nullmol/L)F26null54!4null712null98!0null1839F9-25null67!2null162null88!0null21926null19!3null562null92!0null179F9-26null61!4null692null94!0null11926null14!3null912null91!0null15a9Fa3FaF9%(nullx!s):F(11null1!6null45)；39-(10null4!7null36),9(14null0!3null49)；9-(11null8!7null19),9(13null5!5null65)bFWsd9il(F=0null93,P=0null45)b9nm13b)nullnullLL0Vr=9%iOVrMi1,2b-i0osj,i0o=9/1bABR_?QP$.33bABR4oF,Bo9*a*,oP*#9,o9#,ombao.#oWM,ooKv,ABRobkABRTNP?m,?1Q3,7d,ABRFM1sd9ilb1/%ybyN,8=1c9FFch(4U%i,M1*%MbLYV1Fc9%bw#wQd3|01,b,A4T/C,Vns；9；bSOD|01BT,Pbh,1H5；v5；,YV9TVp$SODb91-Fd,1iCorti54bSODNd1FsbkSODTZ4_=9BS,kV,39FSODcFM1sA,V/=9SODch,4U/=9bNO38=BQ1,NOst%h?3?ZM1bNOnull442null90hS2005M6406nullChinJOtorhinolaryngolHeadNeckSurg,June2005,Vol40,No.6s0Q3NO2,NO-3NO-2,NO-21h=95%NO-3,-,S=NO-3NO-2i(NO-3/NO-2)TNO3zSbk4b+s|NO-3NO-2,NO-2A4T3,_；vlVNO,Tnullmol/LVUbCortiNO3/YVr,sO/(V$Vr,Vv3NO,vNOrBFhM5a6b,=9V_B7,8bMBs9?hTmj,kLNOhM1,i9A*s,39A19bkNOTZ4_=9S,kV,39FNOcFM1sd9il,Vi/=9NOc6,4U/=9b9V49%MbL9TBZ4_=9%BS,kV,i39F9FM1,%(sd9ilbTV,0=9?/bInull本文可能是课题研究的一部分,没有观察这一剂量的地塞米松经鼓室给药后对造模动物(如造成内耳积水的动物模型)的治疗效果b也没有检测不同剂量的地塞米松经鼓室给药对豚鼠耳蜗结构和功能的影响和对造模动物的治疗剂量b如果能补充这两项内容则更具有科学性和完整性bID1GianoliGJ,LiJCnullTranstympanicsteroidsfortreatmentofsuddenhearinglossnullOtolaryngolHeadNeckSurg,2001,125:142null146null2LefebvrePP,StaeckerHnullSteroidprofusionoftheinnerearsuddensensorineuralhearinglossafterfailureofconventionaltherapy:apilotstudynullActa0tolaryngology,2002,122:698null702null3WadaS,MatsuokaS,UrasakEnullQuantitativeanalysisofreversibledysfunctionofbrainstemmidlinestructurescausedbydisturbanceofbasilararterybloodflowwiththeauditorybrainstemresponsenullElectroencephClinNeurophysio,l1998,69:148null159null4BeckmanJS,KoppenolWHnullNitricoxide,superoxide,andperoxynitrite:thegood,thebad,andtheuglynullAmJPhysio,l1996,271:C1424null1437null5MichelO,HessA,BlockW,etalnullLocalizationoftheNO/cGMPnullpathwayinthecochleaofguineapigsnullHearRes,1999,133:1null9null6TakumidaM,AnnikoMnullLocalizationofnitricoxidesynthaseisoforms(NOS,and)inthevestibularendorgansoftheguineapignullORLJOtorhinolaryngolRelatSpec,1998,60:67null72null7TakumidaM,AnnikoM,PopaR,etalnullLipopolysaccharidenullinducedexpressionofinduciblenitricoxidesynthaseintheguineapigor