[毕业设计精品]茶多酚对心肺复苏大鼠生存时间和肾损伤的影响 正式

A1A0A2A4A3A5A6A7A9A8A11A10A12A13A14A15A16A17A18A19A202008A22A21A23A24A25A11A26A27A28A291目录一、论文茶多酚对心肺复苏大鼠生存时间和肾损伤的影响1英文缩略语22中文摘要33英文摘要54论文主体前言8材料与方法10结果13讨论17参考文献23附图265全文小结27二、综述综述茶多酚的药理研究进展28三、致谢39A1A0A2A4A3A5A6A7A9A8A11A10A12A13A14A15A16A17A18A19A202008A22A21A23A24A25A11A26A27A28A292英汉双解缩略词表缩略词英文全称中文全称TPTEAPOLYPHENOLS茶多酚OFROXYGENFREERADICALS氧自由基IRIISCHEMIAREPERFUSIONINJURY缺血再灌注损伤SODSUPEROXIDEDISMUTASE超氧化物歧化酶MDAMALONDIALDEHYDE丙二醛CACARDIACARREST心跳骤停CPRCARDIOPULMONARYRESUSCITATION心肺复苏ROSCRESTORATIONOFSPONTANEOUSCIRCULATION自主循环恢复A30A31A32A33A34A35A36A37A38A39A40A41A42A43A44A45A46A47A48A49A502008A51A52A53A54A55A56A57A58A59A603茶多酚对心肺复苏大鼠生存时间和肾损伤的影响摘要目的各种原因引起的心脏骤停（CARDIACARREST,CA）可导致全身器官严重缺血缺氧，及时有效的心肺复苏（CARDIOPULMONARYRESUSCITATION,CPR）可能使部分患者恢复自主循环（RETURNOFSPONTANEOUSCIRCULATION,ROSC），但与此同时也带G7481各器官G13464G13467的缺血再灌注损伤，严重影响复苏G2530生存时间和生存G17148G18339G452G3926G1321G19489G1314复苏G2530的G8527G1141G10587和G11161G8543G10587G7171目前G2319G4410G11040G7380G1038G1863注的G9921G9869G1055G980G452茶多酚（TEAPOLYPHENOLS，TP）G1867有G5468G5390的G6251氧化G1328G11004，对G4628部缺血再灌注损伤G2172物心G451G14053G451肾G1867有G1457G6264G1328G11004G452心脏骤停与心肺复苏的G17819G12255同G7691可导致全G14053缺血再灌注损伤，茶多酚G7171G2554能G3827G17902G17819G1866G6251氧化G1328G11004G6925G2904CPRG20056G2530G7424文G17902G17819G5326G12447G12390G5699G5627心脏骤停大鼠G8181G3423，对ROSCG2530大鼠G2375G2063G5224G11004TP，G16278G4531TP对血G8981G2172G2159G4410G451生存时间及G12082G13475G2163能的影响，G16278G4531肾脏G5430G5589G4410G6925G2476，G7828G8991肾G13464G13467超氧化物歧化酶（SUPEROXIDEDISMUTASE,SOD）G8975G2159G451丙二醛（MALONDIALDEHYDE，MDA）G2559G18339的G2476化，G16792G1227TP对CPR的影响，G1038G6518G13046G6251氧化G2070G11004G1122G6925G2904CPRG2530器官G2163能的G8847G11115G6564G1391G4466G20576G1393G6466G452方法G17885G11004G1593G5259SD大鼠，体重200400G，10G8712G2524G8707醛G21647G18269（3ML/KG，G14157G14120注G4568），G3853G19393G8680G12661G6566G12661G16837导G12390G5699G5627心脏骤停，8MING2530G13485G1116G14028G3818心脏G6365G2399及G7438G7812G17902G8680G452G6365G23991MIN时G13485G1116肾G990G14158G13044（004MG/KG），ROSC大鼠G13475G19757G14045G2375G2063A61A62A63A64A65A66A67A68A69A70A56A71A72A73A74A75A76A77A78A79A802008A51A52A53A54A55A56A57A58A59A604G19555G7438G13485G1116生理G11428G8712（2ML/KG）G6122TP（10MG/KG），G16278G4531ROSCG25301HG1881G6922缩G2399G451G14310G5364G2399及G5191G3355G2172G14045G2399的G2476化，ROSCG2530大鼠的存G8975时间及复苏G253024HG45148H存G8975G10587，并进行G12082G13475G2163能G16792分G452取存G8975到12HG45124HG45148H大鼠肾脏，常规切片，G16278G4531G11161理G6925G2476，肾G13464G13467匀浆G7828G8991SODG8975G2159G451MDAG2559G18339G452结果1TPG13464与生理G11428G8712G13464在ROSCG25301HG6922缩G2399G451G14310G5364G2399和G5191G3355G2399无显著G5627差异，TPG13464大鼠的生存时间明显长G1122生理G11428G8712G13464（373112HVS243141H,P20MMHG，持续5MIN以G99023；CPR5MING1881G7422能恢复ROSCG1038复苏失败，退出G4466G20576（N4）G452复苏5MING1881恢复ROSC的大鼠被分成两G13464，生理G11428G8712G13464（G13941G19757G14045注G4568生理G11428G8712，2ML/KG,N40），TPG13464（G13941G19757G14045注G4568005TP溶液,10MG/KG,N40），G17842续60MING16772G5417G2172G14045血G2399和心电图G2476化G45260MING2530拔除G2172G19757G14045导G12661并缝G2524伤G2487G452待大鼠出现自主呼吸40次/MIN，停G11004呼吸G74381MING2530血G2399G451心G10587和呼吸状况稳G4462G6122持续好转时拔除G8680G12661G6566G12661，撤离呼吸G7438G452将大鼠放G1849笼中，G16278G453148HG1881存G8975时间（自主循环恢复到自主呼吸消失），24HG45148H的存G8975G10587，并在24HG45148H进行G12082G13475G2163能G16792分G452参照JIAG1257324的G12082G13475G2163能G16792分方法，从意识G451基G7424反G4568G451运G2172G451感觉G451行G1038G4410G12573方面对存G8975大鼠在ROSCG253024HG45148H进行G12082G13475G2163能G16792分，G7643G1946G3926下A231A232A233A234A235A236A237A238A239A240A241A242A243A244A245A246A247A248A249A250A2512008A252A253A254A255A22A241A0A1A2A312G12082G13475G2163能G16792分系统AG980般行G1038缺陷总分19分意识，知觉正常10；昏迷5；无反G52240；觉醒自G2172睁眼3；刺G9620睁眼1；无睁眼0；呼吸正常6；异常（G17819缓G6122G17819快）3；无呼吸0；BG14053G5190G2163能总分21分嗅觉（对食物的反G5224）存在3；G993存在0视觉（G1821刺G9620的头部运G2172）存在3；G993存在0瞳孔对G1821反G5224存在3；G993存在0角膜反G5224存在3；G993存在0惊跳反G5224存在3；G993存在0胡须刺G9620反G5224存在3；G993存在0吞咽吞咽液体G6122固体存在3；G993存在0C运G2172G16792估G5390G5242（总分6分，G5050右各3分）正常3；僵硬G6122软弱1；无运G2172G6122瘫痪0；（G5050右两边单独G16792分，）D感觉G16792估疼痛刺G9620反G5224（总分6分，G5050右各3分）反G5224灵敏3；反G5224弱G6122异常（延展G6122屈曲姿势）1；无反G52240；（G5050右两边单独G16792分）E自主行G1038总分6分；G8505G5589协调正常3分；异常1；G993存在0；G5191衡木行走正常3分；异常1；G993存在0；F反G4568总分12分；正位反G4568，直反G4568正常3分；异常1；G993存在0；拒绝趋地反G4568正常3分；异常1；G993存在0；视觉配G2524正常3分；异常1；G993存在0；转向G16809G20576正常3分；异常1；G993存在0；G癫痫发G1328（痉挛G5627G6122非痉挛G5627）（总分10；）无癫痫10；部分癫痫5；完全癫痫；A4A5A6A7A8A9A10A5A11A12A13A14A1505M,A162CMA171MA18A8A19A20A21A23A24A23A251MA26A27A28A29A30A31A32A23A251M,A33A34A35A11A8A19A20A36A26A37A38A26A27A39A29A30A40A11A8A19A20A41A42A37A38A26A27A43A44A11A24A28A45A46A47A5A42A46A28A46A47A48A49A50A51A52A53A54A14A15A48A55A51A32A56A57A58A59A24A60A61A9A10A5A11A12A1705MA18A62A63A64A21A23A48A23A65A13A66A6715CMA68A56A69A60A61A24A70A71A72A73A5A51A74A75A76A77A48A55A51A32A78A79A80A70A71A81A82A33A83A84A56A59A18A45A85A24A86A87A88A14A46A47A5A52A89A12A90A9145A92A91A15A18A55A51A32A93A28A80A94A33A61A20A95A69A24A231A232A233A234A235A236A237A238A239A240A241A242A243A244A245A246A247A248A249A250A2512008A252A253A254A255A22A241A0A1A2A313G16792分范围G1038080分，0分G1038G8527G1141，80分G1038完全正常，G16792分由两人分别G8991G4462G2530取G5191G3355分值G452分别取ROSCG2530存G8975到12HG45124HG45148H时间G9869的大鼠肾脏，置G11224多聚甲醛中再固G4462G451脱G8712G451石蜡包埋G451常规切片（厚G5242G10384UM），在G1821G19248下G16278G4531G5430G5589G2476化G452另取肾G13464G13467，匀浆，G6365G16809G2070G11430说明操G1328，G18331G11004分G1821G1821G5242计G8991G4462匀浆液超氧化物歧化酶（SOD）G8975G2159和丙二醛（MDA）G2559G18339G45214统计学处理G18331G11004SPSS130软件进行统计分G7524，计G18339资料G11004G3355数G7643G1946差XS表示，比较G11004TG7828G20576G451方差分G7524，计数资料G110042G7828G20576G6122秩和G7828G20576，P005G1038差异有显著的统计G4410意G1053G4522结果21G12390G5699前两G13464大鼠基础参数比较G12390G5699前两G13464大鼠的体重G451心G10587G451血G2399G451体G9213参数比较无明显差异，见表1G452A96A97A98A99A100A101A102A103A104A105A106A107A108XA109A110A111组别茶多酚组（N40）盐水组（N40）体重（G）330189633156959心率（次/分）41842564268312收缩压（MMHG）13612411348219舒张压（MMHG）1037188944200平均动脉压（MMHG）11802051110201体温（A112C）358103630922ROSCG2530血G2399的比较221ROSCG2530，两G13464大鼠的G6922缩G2399比较无显著G5627差异，见图1G452A113A114A115A116A117A118A119A120A121A122A123A124A125A126A127A128A129A130A131A132A1332008A134A135A136A137A138A123A139A140A141A14214A143A97A144A145A146A147A148A149A150A151A152A153A154A98A99A100A101A155A156A157A106A107A158PA159005A160FIGURE1THECOMPARISONOFTHESBPBETWEENTHETWOGROUPSAFTERROSC60MINPA161005A158BLBASELINE,A162A163A164A165A166PRPOSTRESUSCITATION,A167A168A169A170222ROSCG2530，两G13464大鼠的G14310G5364G2399比较无显著G5627差异，见图2G452A171A172A173A174A175A176A177A178A179A180A181A182A183A184A185A186A187A188A189A190A191A192（P005）04080120160200BLPR2PR4PR10PR20PR30PR40PR50PR60TIMEMINSBPMMHGA193A194A195A196A197A198A19504080120160200BLPR2PR4PR10PR20PR30PR40PR50PR60TIMEMINDBPMMHGA193A194A195A196A197A198A195A199A200A201A202A203A204A205A206A207A208A209A210A211A212A213A214A215A216A217A218A2192008A220A221A222A223A224A209A225A226A227A22815FIGURE2THECOMPARISONOFTHEDBPBETWEENTHETWOGROUPSAFTERROSC60MINPA229005,（BLBASELINE,A230A231A232A233A234PRPOSTRESUSCITATION,A178A235A183A170223ROSCG2530，两G13464大鼠的G5191G3355G2172G14045G2399比较无显著G5627差异，见图3G452A171A236A173A174A175A176A177A178A179A180A181A182A183A184A185A186A187A237A238A239A240A190A191A192（P005）FIGURE3THECOMPARISONOFTHEMAPBETWEENTHETWOGROUPSAFTERROSC60MINPA241005A242BLBASELINE,A230A231A232A233A234PRPOSTRESUSCITATION,A178A235A183A17023ROSC时间和存G8975时间比较两G13464大鼠ROSC时间无显著G5627差异G452但TPG13464大鼠的存G8975时间明显长G1122生理G11428G8712G13464（P005），见表2G452A243A172A173A174A175A176A177A178A244A245A246A247A248A244A245A249A191A192XS组别自主循环恢复S存活时间HTP组（N40）996364373112生理盐水组（N40）116349524314104080120160200BLPR2PR4PR10PR20PR30PR40PR50PR60TIMEMINMAPMMHGA250A251A252A253A254A255A252A1A0A2A4A3A5A6A7A9A8A11A10A12A13A14A15A16A17A18A19A202008A22A21A23A24A25A11A26A27A28A2916A51A30A31A32A33A34A44A44P00524G993同时间G9869存G8975G10587比较ROSCG2530，TPG13464大鼠24HG45148H存G8975G10587明显高G1122G11428G8712G13464P001，见表3G452A35A40A36A32A37A38A39A41A42A43A45A46A47A48A49A50组别24H48HTP组（N40）850（N34）400（N16）生理盐水组N40假复苏组N5525（N21）100N5150（N6）100N5A52A58A53A54A55A56A49A50A60A57P00102040608010024H48H生理盐水组茶多酚组假手术组A59A61A62A56A63A38A39A41A42A43A45A46A47A48A49A50FIGURE4THECOMPARISONOFTHESURVIVALRATEAMONGTHETHREEGROUPSSURVIVALRATETIMEA64A65A66A67A68A69A70A71A72A73A74A75A76A77A78A79A80A81A82A83A842008A85A86A87A88A89A74A90A91A92A931725G12082G13475G2163能G16792分比较TPG13464大鼠24HG45148HG12082G13475G2163能G16792分明显高G1122生理G11428G8712G13464（P005），见表4G452A94A61A95A56A63A38A96A97A98A99A100A101A49A50MP25A57P75组别24H评分48H评分TP组5747,6200,68生理盐水组假复苏组350,548000,080A52A54A55A56A49A50A57A57P00526肾G13464G13467G5430G5589G4410G16278G4531261肉眼G16278G4531（1）假复苏G13464G3818G16278无异常，表面G1821滑G451饱满，G17148软，弹G5627好，呈G7275G13430G14406G452（2）TPG13464表面G1821滑G451饱满，G17148G12257软，弹G5627较好，呈G7275G13430G14406；（3）生理G11428G8712G13464G13971G13972，呈G7275G13430G14406，部分可见明显G6967在G11220G7013G452262G1821G19248G16278G4531假复苏G13464肾小G10711结构完好，肾小G12661G990G11394G13466G14002大多无明显G13971G13972，G1146无G2476G5627G451G3363G8527，间G17148G6925G2476G993明显见附图1G452生理G11428G8712G13464G1821G19248可见肾小G10711G14818缩，肾小G12661G990G11394G13466G14002G2476G5627G3363G8527G451脱G14865，肾间G17148G8712G13971见附图2G452TPG13464以肾小G12661G13971G13972G1038主，G990G11394G13466G14002G3363G8527G451脱G14865较G4581，肾小G10711也有G980G4462G12255G5242的G14818缩见附图3G452A102A103A104A105A106A107A108A109A110A111A112A113A114A115A116A117A118A119A120A121A1222008A123A124A125A126A127A112A128A129A130A1311827肾G13464G13467SODG451MDAG8991G4462,见表5G452A132A133A134A135A136A137A138A139A140A141A142A143A144A145A146A142A147A148A149A150A151A152A153A154A155A156A157A153A158A159A153A160A161A162A153MDANMOL/MGPROT12H180203818403614603124H171202416803515602848H201028154014151046SODU/MGPROT12H25366272811683260668824H27256422884456286767248H239466125654812806616A163A135A164A164A149A150A165A166A167A168A167A1333讨论CA和CPRG2530G7438体重要器官心脏和大G14053在结构和G2163能G990G17985G2475的损伤G7171由两个G11161理G19466G8585造成的G452G12544G980G19466G8585G7171在CA期间，全身血液循环停止，各器官严重缺血缺氧，G2563G2172G1114G980系G2027G11161理G2476化G17819G12255缺血缺氧G2530，G13464G13467G14901G14808G12970G451ATP快G17907G13803竭，G12970G18249G16311产物生成和脂G13950G18252类物G17148G3546G12227使G13466G14002G1881G18252中G8614；能G18339G13803竭G2530，离子G8905失G8975，G13466G14002G1881G19060G9394G11053，膜G1881电位G2447极化，NA/CA交G6454异常G6122膜G19463离子G17902G17959开放，G19053进G1849G13466G14002G3698多和G14002G1881G19053G5223G6680G451G17154G2163能阻碍，使G14002浆G19053大G18339G3698G2164；G14002G1881G19053超G17745G2460引起G13459G12902体损伤，而G13459G12902体损伤G13499而引起氧自由基生成G452G12082G13475G13466G14002G19053超G17745还可G1431进G1864G3871G5627G8700基G18252大G18339G18334放，产生G8614G5627反G522410G452G12544二G19466G8585发生在CPR停止和ROSCG2530，G9316G1122缺血再灌注损伤G452多种损害因G13044，G3926氧自由基G451G19053超G17745G451G9826症因子G451血液高G1969G5627G451血G7655G5430成与G5506循环障碍G12573（G12082G13475损害还G9053及到G1864G3871G5627G8700基G18252的G8614G5627G1328G11004）导致心肺复苏成G2163者在心脏方面发生心G13920顿G6245G451心脏G6922缩和G14310G5364G2163能减退；在中G7542G12082G13475方面发生G3363G8527G451G1951G1141，从而导致早期复苏成G2163者G7380终G8527G1141G6122持久G5627G12082G13475系统G2530遗症G452A169A170A171A172A173A174A175A176A177A178A179A180A181A182A183A184A185A186A187A188A1892008A190A191A192A193A194A179A195A196A197A19819氧自由基，G3926超氧化物G451羟氧自由基G7171引起CA/CPR心G451G14053G20330次和二次损伤的主要因G13044G1055G980G452心跳停G2350患者G13475G6262救，恢复自主循环G2530，G15441G9994心G14053G1391血G451G1391氧有G6164恢复，但G13459G12902体正常G2163能G4590G7422恢复，氧G1391G5224G8712G5191超G17819G1114G13459G12902体能G3827利G11004的G8712G5191，以G14279氧自由基G17902G17819多个G17896径G10202发G5347生成（1）缺血使G13459G12902体DNAG2475损，呼吸G19154复G2524物电子G1268G17894G2475G11784G3363，G21656G13044G14158G3040G2616二G7692G14539G18252G1393G17194G5627复G2524物G17896径被G17819G5242利G11004，次G21656G3040G2616转化G1038G21656G3040G2616，G21656G3040G2616分G16311G1038G4627G13044反G5224中G18129G1288G19555超氧G19464离子（O2）G18334放G452（2）G13466G14002G19053G1881G8981G3698G2164，G16314发G14469生四G9923G18252G1207G16886，G14469生四G9923G18252转化G1038PGH2中G18334放O2G452（3）G12082G13475G3423G980氧化G8706G2524酶G9620G8975，使G17819G1134G11825G18252G11428（ONOO）产生G452（4）G12082G13475系统小G14026G17148G13466G14002G451血液G11345G13466G14002G9620G8975也可产生氧自由基G452另G980方面，G1881G9316G5627G6251氧化物G17148大G18339消G13803，使自由基生成/G9177除严重失衡，引发自由基损伤G5627级联反G5224，表现G1038（1）使膜脂G17148G17819氧化，G13466G14002结构G451膜离子转运和G4643障G2163能被G11784G3363G452（2）G16837导DNAG451RNAG451多G12970和G8700基G18252G12573大分子物G17148交联G2476G5627G6122断G16022，G13499而引起G13466G14002G8527G1141和（G6122）G1951G1141G452（3）G9620G8975G11345G13466G14002和血G12661G1881G11394G13466G14002的G9907G18244G14030G14158G3040G2616二G7692G14539G18252G11979G18252G11428氧化酶，引起G980系G2027血G12661G5627G11161理反G5224G11345三G9923和血小G7507G9620G8975因子生成，G1881G11394G12908附因子（PG17885G6333G13044G451G13466G14002间G12908附分子1）表G17810G3698多，G6251G12908附因子失G8975，G1431进G5506血G7655G5430成，G5506循环障碍11G452因此，G6251氧化G451阻断氧自由基级联反G5224G7171CPRG8847G11115的G980G7477G5617G17347G452TPG7171茶叶中多羟基酚类化G2524物的总G12228，主要成分G1038G1811茶G13044类G451G21656G18242类G451G21656G18242G18267类G451G14469G19750G13044类G451酚G18252G451缩酚G18252类及聚G2524酚类G12573,G1866中G1811茶G13044类约G2356总G18339的6580G452G1811茶G13044类主要包括表G1811茶G13044ECG451表G8821食子G1811茶G13044EGCG451表G1811茶G13044G8821食子G18252G18243ECG和表G8821食子G1811茶G13044G8821食子G18252G18243EGCG4种物G17148,G1866中EGCG约G2356G1811茶G13044的50G5050右12G452TP能G3827G17902G17819多种G17896径产生G6251氧化G1328G1100413（1）直接G9177除氧自由基，TP分子中带有多个G8975G5627羟基（G713OH），G7143氧化成G18272类而G6564G1391G17148子，有效地G9177除超氧G19464离子O2G451羟自由基OHG451单G13459G5589氧1O2及G17819氧化G8694H2O2及中间产物脂G17819氧自由基A199A200A201A202A203A204A205A206A207A208A209A210A211A212A213A214A215A216A217A218A2192008A220A221A222A223A224A209A225A226A227A22820和G9931氧自由基而终止G19154G5347反G5224G452（2）G15747G2524金属离子，在中G5627G6122G18252G5627G7477件下,TP的邻位二酚羟基对G17819G9205G5589金属离子有G980G4462的络G2524G1328G11004,从而阻止金属离子对G8975G5627氧G12573自由基的生成G452（3）G9620G8975G13466G14002G1881G6251氧化G19462G5493系统G452G6117G1216在ROSCG2530G2375G2063G19757G14045注G4568TP，与生理G11428G8712G13464比较，TP对复苏G2530大鼠G2172G14045血G2399影响G993大，但能G3827显著地延长复苏G2530大鼠生存时间和G6564高24HG45148H生存G10587，并且能G3827G6564高生存大鼠的G12082G13475G2163能G452G7424研究结果表明G1114TP对CPR的G6251氧化G8847G11115效果，G6564示TPG17902G17819G9177除CPRG2530G17819G18339生成的氧自由基和/G6122阻断氧自由基级联反G5224及G3698G5390G7438体G6251氧化系统G2163能多方面的G1328G11004，减轻CPRG2530G7438体器官的缺血再灌注损伤，有效地G6564高CPR救G8847效果G452G17837G980结果也G6915持G1114G1866G1194CPRG2530G6251氧化研究G6164G6564示的意G1053XUYG1257314发现转基因G17819氧化物G4708化酶高表G17810大鼠CA/CPRG2530G14053血G8981G18339恢复到正常G8712G5191的时间G11713G1122G18338生大鼠，而且生存状G5589也G1260G1122G18338生大鼠G452CERCHIARIELG1257315对G12390G5699G16837发CA的G4466G20576G10411G4466G7057CPR时，G19757G14045注G4568G17819氧化物歧化酶配G2524G8847G11115，能G3827减小复苏G2530G14053血G8981G18339的G8886G2172和G6564高G13942体感觉G2808起能G2159G452DEZFULIANCG1257316G16278G4531到G11825G18252G11428能G3827调节再灌注早期G13459G12902体G8975G5627氧的产生，阻G9394急G5627心G2163能衰竭和减轻存G8975者G12082G13475损伤G12255G5242，G6564高CA小鼠CPRG2530存G8975G10587G452比较吸G1849G993同G2559氧G18339G8680体对CA/CPRG2530G12082G13475损伤的影响的G4466G20576也表明G1114氧化与G6251氧化的G11468G1126G1863系高氧G13464（FIO210）G10411复苏G253012和24H小时的G12082G13475损伤明显大G1122常氧G13464（FIO2021）和高氧G6251氧化G2070G13464，G6564示G6251氧化G2070在G980G4462G12255G5242G990可以减轻高氧造成的G12082G13475损害17G452甲G11982G18252G18243G7171G980种膜脂G17148G17819氧化G6245制G2070，能G3827减轻G4466G20576G2172物的缺血再灌注损伤，G1431进器官G2163能G451包括G12082G13475G2163能的恢复18G452对电刺G9620G16837发G4472G20088致CA的G10411G8181G3423，行常G9213G451常规CPR，ROSCG2530G19757G14045G13485G1116脂G17148G17819氧化G6245制G2070（G8700基类固G18267U74006F），与柠檬G18252对照G13464比较，显示复苏G253024H存G8975G10587G6564高，G5191G3355生存时间延长，复苏G25301，2，24H呈现更好的G12082G13475G2163能G6363G7643，复苏G253012H更G1314的血浆脂G13950G18252G17819氧化物G8999G5242，复苏G25302，3，6H更高的血浆维生G13044EG8999G524219G452A199A200A201A202A203A204A205A206A207A208A209A210A211A212A213A214A215A216A217A218A2192008A220A221A222A223A224A209A225A226A227A22821研究TP对G993同器官CPRG2530缺血再灌注损伤的影响G7171G6518明G1866G6564高生存效果G7438制的重要工G1328，TP对心脏G451G12082G13475系统的G1457G6264G1328G11004由课题G13464G1866G1194成员进行，G7424文从显G5506结构和SODG451MDA方面G6518讨G1114TP对CPRG2530肾脏的G1457G6264G1328G11004G452G4466G20576结果表明，TP对CRP大鼠肾脏结构G1867有G1457G6264G1328G11004，主要表现G1038减轻肾小G12661G990G11394G13466G14002G3363G8527G451脱G14865，减轻间G17148G8712G13971G452此G1328G11004有助G1122G7438体重要器官心脏和大G14053的恢复，G6564高CPR大鼠的生存时间和24HG45148H的生存G10587G452G15441G9994肾G13464G13467SODG8975G5627G451MDAG2559G18339在TPG13464G451假复苏G13464和生理G11428G8712G13464G1055间G8821有明显差异，但G993能G2554G4462TP抵G6251CPRG2530肾脏结构的G11161理G6925G2476与G1866G6251氧化G8975G5627有G1863G452在缺血再灌注损伤G11161理G1328G11004中，氧自由基的损害G7171多方面的，除G1114削弱体G1881G6251氧化系统G2163能（SODG7171G1866中的G980个还原酶）G451氧化G993饱和脂G13950G18252（MDAG7171G1866中的G980个氧化产物）G3818，还G17902G17819G1866它G17896径产生损害G1328G11004G452氧自由基使G1881G11394G13466G14002和黏膜G13466G14002G17902透G5627G3698G2164，G9826G5627G13466G14002渗出G451浸润；G9620G8975中G5627G12902G13466G14002产生G980系G2027G8614G5627因子,使G13464G13467损伤；直接损伤肾小G10711毛G13466血G12661G1881G11394G13466G14002,使肾小G10711系膜G13466G14002和肾小G12661G990G11394G13466G14002G2476G5627G451G3363G852720；G17902G17819G13466G14002信号转导G17902G17347引发G13466G14002G1951G114121G452因此，要G6518明TP对CRP大鼠肾脏结构G1457G6264G1328G11004的G7438制，还有大G18339的工G1328需要G2447G1328G452G2172物G4466G20576呈现G1114G6251氧化G2070对CPRG2530心G451G14053的G1457G6264G1328G11004的G8847G11115G1227值，但却G7422得到临床G5224G11004的肯G4462，主要原因G980G7171G2172物G4466G20576研究G11004的G6251氧化G2070多G7171化G4410品而非临床G11004药，故无临床报G17959；二G7171G6251氧化G2070G8847G11115的时间窗G11713暂G452大G18339氧自由基G1122CA和ROSCG2530瞬间产生和瞬间发生损害G1328G11004，G6251氧化G2070须在CPR期间G6122ROSCG2530及时使G11004才会有明显效G5224，而临床G4466G7057CPRA199A200A201A202A203A204A205A206A207A208A209A210A211A212A213A214A215A216A217A218A2192008A220A221A222A223A224A209A225A226A227A22822时G993可能G1328G4466G20576G5627G16278G4531，G980些G6251氧化G2070对CPRG2530心G451G14053的G1457G6264G1328G11004G16278G4531也G7171延迟G5627，故G11115效G993确G4462G452因此，从现G11004的疾G11161G19462G8847品中寻找能G3827G6925G2904复苏G20056G2530的有效G6251氧化物G5224更G1867有G4466际G5224G11004G1227值G452TP能G3827G6925G2904常规CPRG2172物的救G8847效果，取决G1122G1866高效的G6251氧化G8975G5627G451较高的G4445全G5627（G1038G3837G9994物G17148）G451G13485药的及时G5627（G6117G1216在ROSCG2530G2375G2063使G11004）G452G6117G1216的工G1328丰富G1114CPR研究G1881容，G1038G6251氧化G2070G5224G11004G1122CPRG6564G1391G1114G4466G20576研究G1393G6466G452A199A200A201A202A203A204A205A206A207A208A209A210A211A212A213A214A215A216A217A218A2192008A220A221A222A223A224A209A225A226A227A22823参考文献1方芳,崔志G9177,韩永晶茶G