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高效液相色谱法测定胸腺肽1的氨基酸组成A1A0A3A2A3A2A5A4A6A7A8A9A10A11A12A13A14A15A16A17A18A191A20A21A22A23A24A25A26A27A28A29A30A31OPAA32A33A34A35A36A37A38A39A40A21A22A23A24A25A26A41A42A34A43AGILENTZORBAXECLIPSEAAA46150MM,35A44MA45A46A47A48A43AA48A4940MMOL/LA50A23A51A52A53A20A54A55A56A45PH72,BA48A49A57A58A59A60A61A59A54A6245A5945A5910A45A30A31A63A64A65A66A45A46A47A48BA6717MINA68A690A70A71A7257A70A73A46A74A4320MLMIN1A73A75A39A76A77A43338NMA26A78A79A29A80A81A56A48A41A42A38A39A40A82A21A22A23A20A83A84A85A86A4901A875MOL/MLA45R0999A45A88A89A38A90A91A84A92A93A94A64A95A96A97RSD2A70A98A26A78A99A29A80A81A56A48A41A42A38A39A40A100A101A102A191A20A21A22A23A24A25A45A89A38A103A104A45A105A106A107A108A26A109A110A111A112A113A1141A115A116A117A118A119A120A121A122A123A124OPAA125A126A127A128DETERMINATIONOFAMINOACIDCOMPOSITIONOFTHYMOSIN1BYHPLCABSTRACTOBJECTIVETOESTABLISHHPLCMETHODFORDETERMINATIONOFAMINOACIDCOMPOSITIONOFTHETHYMOSIN1METHODOPAREAGENTPRECOLUMNDERIVATIZATIONMETHODWASUSEDCOLUMNAGILENTZORBAXECLIPSEAAA46150MM,35A129MMOBILEPHASEA40MMOL/LNA2HPO4,PH72MOBILEPHASEBCH3OHA130CH3CNA130H2O45A13045A13010,THEGRADIENTELUTIONMETHODWASUSEDTHATTHEMOBILEPHASEBWASINCREASEDFROM0A131TO57A131INSEVENTEENMINUTESFLOWRATE20MLMIN1DETECTORWAVELENGTH338NMRESULTSTHEREISAGOODLINEARRELATIONSHIPWITHEVERYAMINOACIDWITHTHERANGEOF01A1325MOL/MLR0999,THEREPEATABILITYANDPRECISIONOFTHEMETHODISGOODRSD2A131CONCLUSIONTHEMETHODTODETERMINEAMINOACIDCOMPOSITIONOFTHYMOSIN1BYHPLCISSIMPLEANDACCURATEKEYWORDSTHYMOSIN1,AMINOACID,HPLC，OPAREAGENT,DERIVATIZATIONA133A134A135A1361A137THYMOSIN1,T1A138A139A133A134A140A141A142A143A144A145A146A147A148A149A135A150A151A152A153A154A150TA155A156A157A158A142A159A160A1611966A162A152A163GOLDSTEINA1641A165A166A167A168A169A170A171A172TF5A173A174A175A176A177A178A179A180A181A182A183A184A185A186A187A188A189A187A190A191A178A192A193A177TF5A194A195A188A196A197A198A187A19910A2001000A2012A202A203A204A205A190A206A207A208A189A209A210A211A212A213A183A214A215A178A216A217A218A219A220A221A222A196A178A223A224A209A210A211A212A183A214A215A178A225A226A218A219HPLCA227A224A207A208A189A171A228A188A174A229A230A231A232A233A234A235A236A2021A237A238A239A240A241A238A239A240A241AGILENT1200HPLCG1322ADEGASSERG1311AQUATPUMPG1329AALSG1316ATCCG1314BVWDA242A243A244A245A246A247AGILENTCHEMSTATIONB0301A248A249A250A251A252A253A242A243A244A245A246A254A247A255A43A33A46A0A1A2A248A249A3A253A159A4TEDIAA245A246A254A247A161A5A6A162A163A7A8A162A164A9A7A8A162A166A7A8A162A167A7A8A162A168A7A8A162A169A7A8A162A170A7A8A10A11A170A7A8A79A172A12A13A14A15A16A17A18A19A20A21A22A80A23A180A169A7A8A79A149A14A150A21A22A80A23A139A24A25A26A27A28A29A30A31A23A32A34A35A361A79A183A37A38080101A162080102A162080103A39A70A21A22A40A122A80A412A42A44A45A47A48A49A50A51A52A53A54A55A42A44A45A47A49A50A51A52A56A57A58A59AGILENTZORBAXECLIPSEAAA46150MM,35A60MA61A62A63A64A59AA64A6540MMOL/LA66A67A68A69A71A72A73A74A75A76PH72A77BA78A81A82A83A84A85A86A84A87A8845A8445A8410A76A89A90A91A92A93A94A76A95A96A78BA9717MINA98A990A100A101A10257A100A103A95A104A10520MLMIN1A77A106A107A108A109A105338NMA103A110A111A105A187A112A189A103A89A90A110A113A114A115A116A117A118A76A190A119A120A121A123A90A124A195A116A125A126A127A128A76A142A129A130A116A131A132A133A126A134A125A135A114A115A202A136A137A76A125A126A144A127A128A138A140A105A209A141A99A209A184A143A145A146A147A212A148A151A152A215A216A217A133A153A154A155A156A112A148A187A188A157A215A158A160A217A133A87A165A155A76A90A209A112A188A157A215A158A160A171A224A202A173A174A226A175A81A209A112A148A212A76A228A176A177A212A141A99A209A209A184A143A145A146A147A112A148A151A152A215A126A134A230A141A231A97A178A179A145A234A235A124A230A152A215A76A181A182A104A92A76A212A236A187A141A185A186A112A148A151A191A192A151A141A99A212A184A143A145A146A147A112A148A112A152A215A216A87A239A141A99A230A184A143A145A146A147A209A152A215A216A240A193A194A196A197A156A112A148A187A188A157A215A158A160A217A133A153A154A155A145A198A230A244A245A132A199A133A200A201A203A204A82A205A156A240A193A194A177A206A209A112A188A207A158A188A215A177A103A250A141A208A210A211A213A214A218A219A220A221A222A223A225A227A229A232A223A233A237A251A238A241A242A243A213A246A247A248A249A248A221A222A252A253A254A255A238A55A214A242A243A213A246A247A220A230A221A222A252A41A209A248A238A231A208A210A211A213A214A218A219A209A251A221A222A223A225A227A229A232A223A230A237A209A209A238A144A0A247A211A1A2ALAA238A163A1A2THRA238A167A1A2SERA238A164A165A1A2ASPA238A166A1A2LYSA238A168A1A2GLUA238A170A1A2LEUA238A171A170A1A2ILEA238A169A1A2VALA16A3A4A5A6A7A223A801MOL/LA9A2A42A10A11A12A131MLA111A1410MOLA15A17A18A19A20A33A155A21A22A23A24A25A123A20A26A27A28A29A30A31A32A79NVAA80A22A23A24A142A34A33A35A36A37A38A391MLA111A1410MOLA15A19A20A33A155A21A40A43A25A123A20A26A75A44A34A28A40A43A25A123A20A45A22A23A24A25A123A20A641MLA33A4610MLA34A254A111A33A3501MOL/LA47A32A19A20A48A49A58A50A51A33A52A53A33A155A21A22A23A24A19A20A26A139A54A113A51A56A207A57A59A571A31A60A32A61A62A142A195A63A65A66A67A68ASPGLUSERTHRALAA54A113A51A693761109746603A67A68VALNVAILELEULYSA54A113A511270211432212193A701HPLCA71A72A73A74A76A73A74A70A77FIG1THESUITABILITYTESTOFHPLC3A78A81A8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