[生物医药论文精品]半红树植物黄槿hibiscus_tiliaceus化学成分研究

半红树植物黄槿HIBISCUSTILIACEUS化学成分研究A1A0A2A3A4A5A6A7A8A9A10A11A12A13A14A15A16HIBISCUSTILIACEUSLA17A18A19A20A21A22A23A24A25A24A25A26A27A28A29A30A31A32SEPHADEXLH20A33A29A30A31A32HPLCA34A35A36A37A30A31A38A30A31A22A39A40A41A42A43A44A45A18A46A47A48A49A50A49A51A52A53A54A55A22A39A56A5710A58A19A59A54A23A60A61A19A62A63A64A65A31A22A66A67A68A69A70A71A18A72A73A23A74A7510A58A19A59A54A18A72A73A22A76A77A78A79A80A81A82A83A17A32A84A85A44A86A87A88A82A89A17A32A90A91A92A93A94A88A82A95A17A32A96A97A98A88A82A99A17A32A100A101A102A88A82A103A17A32A98A1044A943A81A82A105A17A32A98A1044,22A87A943A81A82A106A17A32A107A108A1044,6,814,22A109A943A81A82A110A17A32A90A111A104A88A82A112A17A64A98A104A88A82A113A17A23A74A114A115A116A117A118A119A120A119A121A122A123A124A125A126A127A128A129A117A130A131A132A133A134A135A136A137A138A139A140A141A142HIBISCUSTILIACEUSA143A144A145A146A147STUDIESONCHEMICALCONSTITUENTSOFHIBISCUSTILIACEUSWANGZHONGZHAO1,TANGXVLI2,LIGUOQIANG1ABSTACTOBJECTIVESYSTEMATICALLYSTUDYTHECHEMICALCONSTITUENTSOFHIBISCUSTILIACEUSMETHODSTHEISOLATIONANDPURIFICATIONOFCOMPOUNDSWEREDEPENDEDONSILICALGEL,SEPHADEXLH20ANDHPLCMETHODS,ANDTHEIRSTRUCTURESWEREIDENTIFIEDBYTHEIRPHYSICALANDSPECTRALDATARESULTS10COMPOUNDS,FRIEDELINA148,PACHYSANDIOLA149A150A151,GLUTINOLA152,LUPEOLA153,GERMANICOLA154,STIGMAST4EN3ONEA155,STIGMASTA4,22DIEN3ONEA156,ERGOSTA4,6,814,22TETRAEN3ONEA157,A158SITOSTEROLA159A160STIGMASTEROLA149A161A151A162WEREISOLATEDANDELUCIDATEDCONCLUSIONCOMPOUNDSA152A163A153A163A154ANDA157WEREFIRSTLYISOLATEDFROMTHISSPECIESKEYWORDSHIBISCUSTILIACEUSLCHEMICALCONSTITUENS半红树植物黄槿（HIBISCUSTILIACEUSL），为锦葵科MALVACEAE木槿属HIBISCUS植物，多生于滨海地区，为海岸防沙、防潮、防风之优良树种，主要分布于中国广东、菲律宾群岛、太平洋群岛、南洋群岛、印度等地。黄槿为全国中草药汇编收录的红树林药用植物。药用部分为叶、树皮和花。其性甘、淡、微寒、具有清热解毒、散瘀和消肿之效用，民间用来治木薯中毒1。长期以来，关于黄瑾的化学成分研究报道较少2,3,4,5。本文对采自海南的半红树黄槿的枝叶进行了较系统的化学成分研究，通过各种色谱分离手段，共G14731G548310G1022G2345G1319化G2524物，G1393G6466化学和G1821谱G7053G8873G11842G4462了G1194G1216的G13479G7512（图1）G726木G7655G18242（G265）、G12893G15134黄G7484G1120G18267（G266）、G163G16G12908G19677G9923G18267（G267）、G13713G6171G16922G18267（G268）、G7097G13831G7376G18267（G269）、G16922G110264G99233G18242（G270）、G16922G110264,22G1120G99233G18242（G271）、G21626G16294G110264,6,814,22G3247G99233G18242（G272）、G163G16907G11026G18267（G273）和G16922G11026G18267（G274）。其中，化G2524物G267、G268、G269和G272为G20330G8437G1186G16825植物中分离G5483G2052。OHOA164A165A166A167A168OA169A170A171A172A173A174A175A176A177A178A176A177A178A179A179A180A181A181A182A183A184A182A183A182FIG1STRUCTURESOFCOMPOUNDSIA185X1仪器与材料G59G23G3G3423G7186微G10088G9869G1214，G9213度G7422G7669G8503G727G7097本JEOLJNMECP600G7692G11925共G6403G1214G727G13666国G4445G6475G12741100G20652效G9094G11468色谱G1214G727SEPHADEXLH20和G4630G7524用G11801G14026分G2047为PHARMACIAG1856G2508和G19750岛海洋化G5049G2390G1147G2709。G5132用G16809G2070G3355为国G1147分G7524G13443。G3半红树植物黄槿G21G19G19G25G3G5192G26G7388采自海南，种属G11013海南东G4540G9219红树林G1457G6264区G19059G6177G14647G2173G10714研究G2604G18504G4462。G7643本G4396G6930于中国海洋G3835学G2319药学G19510G3837G9994药物化学G980G4472。G32提取和分离风G5190的黄槿枝叶11KGG12893G11874，G5049G1006甲G18267冷浸提取3G8437，减压浓缩挥去溶G2070，脱盐，G5483粗提物浸膏336G。用适量蒸馏水混悬，G1393G8437石油醚、乙酸乙酯、G8503丁G18267萃取，G5483石油醚提取物83G乙酸乙酯提取物40G8503丁G18267提取物45G。TLC（薄G4630色谱）比较后，G2524并石油醚乙酸乙酯提取物。石油醚乙酸乙酯提取物进行G8503G11468G11801G14026色谱分离，石油醚丙G18242梯度洗脱，G5483FR18部分。FR13部分通过反复G11801G14026柱色谱和SEPHADEXLH20凝G14026柱色谱和反G11468HPLC分离G13443化，G5483化G2524物G265（10MG），G26650MG，G26720MGG2686MG，G26986MG，G27010MG，G2715MG，G27267MG，G27337MG，G27458MG。3结构鉴定化G2524物G265G726无色针状G13479晶，MP261263，10硫酸G7186浅黄色。1HNMR600MHZ,CDCL32391H,DDD,J136,51,22HZ,H2A,2311H,DD,J132,73HZ,H2B,2251H,Q,J66HZ,H4,1971H,M,H1A,1751H,M,H6A,1681H,M,H1B,1183H,S,H28,1053H,S,H27,1013H,S,H26,1003H,S,H29,0953H,S,H30,0883H,D,J66HZ,H23,0873H,S,H25,0723H,S,H24。以上数G6466与文献6对照基本G980致，G18504G4462化G2524物G265为木G7655G18242。化G2524物G266G726无色针晶，MP290291，10硫酸G7186紫色。1HNMR600MHZ,CDCL33991H,Q,H2,3541H,T,H2,1831H,TD,H1,1611H,M,H1,1173H,S,H28,1013H,S,H26,0993H,S,H27,0943H,D,J72,H23,0943H,S,H24,0943H,S,H30,0853H,S,H25。以上波谱数G6466与文献7对照基本G980致，G18504G4462化G2524物G266为G12893G15134黄G7484G1120G18267。化G2524物G267G726无色针晶，MP218219，10硫酸G7186红色。1HNMR600MHZ,CDCL35631H,BRD,J61HZ,H6,3471H,T,J28,H3,1163H,S,H24,1143H,S,H28,1093H,S,H23,1043H,S,H29,1003H,S,H26,0993H,S,H27,0953H,S,H30,0853H,S,H25。以上波谱数G6466与文献8对照基本G980致，G18504G4462化G2524物G267为G163G16G12908G19677G9923G18267。化G2524物G268G726无色针晶，MP21402150，10硫酸G7186红色。1HNMR600MHZ,CDCL34691H,BRS,H29A,4571H,BRS,H29B,3181H,DD,J116,50HZ,H3,2371H,M,H19,1683H,S,H30,1033H,S,H26,0973H,S,H23,0943H,S,H27,0833H,S,H25,0793H,S,H28,0763H,S,H24。以上波谱数G6466与文献9对照基本G980致，G18504G4462化G2524物G268为G13713G6171G16922G18267。化G2524物G269G726白色G13479晶，MP175178，10硫酸G7186红色。1HNMR600MHZ,CDCL34861H,S,H19，3201H,DD,J115,62HZ,H3,1083H,S,H30,1023H,S,H29,0973H,S,H28,0946H,S,H26、27,0883H,S,H24,0773H,S,H25,0753H,S,H23G72713CNMRC1428C18,1297C19,790C3,555C5,512C9,433C14,408C8,389C4,13,384C1,377C16,373C22,372C10,346C7,344C17,333C21,323C20,313C29,292C30,280C23,275C15,274C2,262C12,253C28,211C11,183C6,167C26,161C25,154C24,146C27。以上数G6466与文献10对照基本G980致,G18504G4462化G2524物G269为G7097G13831G7376G18267。化G2524物G270G726白色针晶，MP15501570，10硫酸G7186紫红色。1HNMR600MHZ,CDCL35721H,S,H4,1193H,S,H19,0923H,D,J64HZ,H21,0853H,T,J81HZ,H29,0833H,D,J72HZ,H27,0813H,D,J69HZ,H26,0713H,S,H18。以上数G6466与文献11对照基本G980致,G18504G4462化G2524物G270为G16922G110264G99233G18242。化G2524物G271G726无色针晶，MP115116，10硫酸G7186紫红色。1HNMR600MHZ,CDCL35721H,S,H4,5151H,DD,J154,83HZ,H22,5021H,DD,J154,88HZ,H23,1183H,S,H19,1013H,D,J66HZ,H21,0853H,D,J61HZ,H27,0803H,T,J77HZ,H29,0803H,D,J61HZ,H26,0723H,S,H18。以上波谱数G6466与文献12对照G980致，G18504G4462化G2524物G271为G16922G110264,22G1120G99233G18242。化G2524物G272G726淡黄色针状G13479晶，MP110113，10硫酸G7186桔黄色。1HNMR600MHZ,CDCL36611H,D,J95HZ,H7,6031H,D,J95HZ,H6,5741H,S,H4,5242H,M,H22、23,1063H,D,J67HZ,H21,1003H,S,H19,0963H,S,H18,0933H,D,J68HZ,H28,0853H,D,J80HZ,H27,0833H,D,J71HZ,H26。以上数G6466与文献13对照G980致,G18504G4462化G2524物G272为G21626G16294G110264,6,814,22G3247G99233G18242。化G2524物G273G726白色针晶，MP139140，10硫酸G7186紫色。1HNMR600MHZ,CDCL35351H,D,J49HZ,H6,3521H,M,H3,1013H,S,H19,0923H,D,J66HZ,H21,0853H,T,J72HZ,H29,0833H,D,J66HZ,H27,0813H,D,J61HZ,H26,0683H,S,H18。以上波谱数G6466与文献14对照基本G980致,G18504G4462化G2524物G273为G163G16907G11026G18267。化G2524物G274G726白色针状G13479晶，MP154156，10硫酸G7186紫色。1HNMR600MHZ,CDCL35351H,M,H6,5161H,DD,J154,83HZ,H22,5021H,DD,J154,88HZ,H23,3521H,M,H3,1023H,D,J66HZ,H21,1013H,S,H19,0853H,D,J66HZ,H26,0803H,T,J77HZ,H29,0803H,D,J66HZ,H27,0703H,S,H18。以上波谱数G6466与文献15对照G980致，G18504G4462化G2524物G274为G16922G11026G18267。REFERENCES1LINPTHEMEDICINALMANGROVEPLANTSINOURCOUNTRYJCHINJMARDRUGSA186A187A188A189A190A191A192A193,1984,3445512LILY,HUANGXS,SATTLERI,ETALSTRUCTUREELUCIDATIONOFANEWFRIEDELANETRITERPENEFROMTHEMANGROVEPLANTHIBISCUSTILIACEUSJMAGNETICRESONANCEINCHEMISTRY,2006,4466246283CHENJJ,HUANGSY,DUHCY,ETALANEWCYTOTOXICAMIDEFROMTHESTEMWOODOFHIBISCUSTILIACEUSJPLANTAMEDICA,2006,72109359384NAIRAGR,SANKARASS,NARAYANASMGLYCOSIDESFROMTHEFLOWERSOFHIBISCUSTILIACEUSJJOURNALOFSCIENTIFICANDINDUSTRIALRESEARCH,SECTIONBPHYSICALSCIENCES,1961,20B5535545SANKARASS,NARAYANASMPIGMENTSOFTHEFLOWERSOFHIBISCUSTILIACEOUSJJOURNALOFSCIENTIFICINDUSTRIALRESEARCH,1961,20B1331346LINC,CAOPX,LIANGGYSTUDIESONCHEMICALCONSTITUENTSOFKALIMERISINDICAJCHINPHARMJA194A195A196A197A198A199A2002006,4142512537CRISTINAM,FATIMAJ,REGINAT,ETALSYNTHETICSECOFRIEDELANEANDFRIEDELANEDERIVATIVESASINHIBITORSOFHUMANLYMPHOCYTEPROLIFERATIONANDGROWTHOFHUMANCANCERCELLLINESINVITROJJNATPROD,2001,6410127312778LIC,YUEDK,BUPB,SUNYFCHEMICALCONSTITUENTSFROMROOTSOFARDISIAPUNCTATACHINJCHINMATERMEDA194A195A194A196A198A199,2006,3175625659ZHOULH,SUNQS,QIAOLC,LIUJPRELIMINARYSTUDYONCHEMICALCONSTITUENTSINACTIVESECTIONOFLEAVESOFQUERCUSVARIABILISBLUMEJJSHENYANGPHARMUNIVA201A