毕业论文 荧光性双层膜的制备及其在超痕量汞离子检测中的应用

学士学位论文毕业论文（科学研究报告）题目荧光性双层膜的制备及其在汞离子检测中的应用院系材料科学与工程学院专业环境工程专升本届别学号姓名指导老师教授教务处二零零五年六月制学士学位论文目录一前言5二实验部分621实验仪器与试剂6211仪器6212试剂622实验内容7221自组装膜的制备72211组装机理72212CYS/AU的制备72213HOX/CYS/AU自组装膜的制备72228羟基喹啉铝（8羟基喹啉汞）的合成8223ALOX3/CYS/AU的制备8224自组装膜荧光光谱测定8225电化学实验9226膜的结构表征9三结果与讨论9318羟基喹啉和8羟基喹啉铝溶液溶剂、激发波长和PH的选择9311溶剂的选择93128羟基喹啉和8羟基喹啉铝溶液的激发波长的选择103138羟基喹啉和8羟基喹啉铝溶液PH值的选择1032自组装膜优化条件的选择113211半胱胺酸在金表面上自组装的电化学表征113212组装DL半胱氨酸浓度的选择123213组装DL半胱氨酸时间的选择123214组装DL半胱氨酸PH值的选择13322CYS/AU电极酸化时间和浓度的选择133238羟基喹琳荧光物质及其金属螯合物的组装133231确定荧光物质的激发波长133232浸泡时间与荧光物质浓度的选择143233PH值的选择1433自组装双层膜的荧光光谱1534自组装膜的表征16341电极荧光光谱表征16342膜的结构表征16358羟基喹啉铝、8羟基喹啉溶液及自组装膜稳定性能的比较17351溶液荧光强度的比较17352稳定性的比较1736HG2的荧光传感分析18361汞离子的荧光传感的分析特性18四结论20五参考文献21学士学位论文ABSTRACTSELFASSEMBLEDMONOLAYERSAMSCANBEDESIGNEDACCORDINGTOOURDEMANDINMOLECULARLEVELA1ITHASMANYMERITSWHICHDONTEXITINOTHERMETHODSNOWSAMSHASBEENEXTENSIVEAPPLIEDINELECTROANALYTICALCHEMISTRYBUTITISFEWREPORTEDABOUTITSAPPLICATIONINFLUORESCENCEANALYSISFLUORESCENCEANALYSISISANIMPORTANTANDEFFECTIVESPECTRUMANALYSISA1IFWECANCOMBINECOMBINESAMSTECHNOLOGYWITHFLUORESCENCEANALYTICALMETHODANDDEVELOPANOTHERINTERFACEFLUORESCENCEANALYTICALMETHODA1ITWASEFFECTIVEINDIMINISHINGTHESAMPLEVOLUMEWITHAVIEWOFONCHIPANALYSISA1THEREFOREHASPROMISINGPROSPECTSINFLUORESCENCEANALYSISANDCHEMICALSENSORTHEFLUORESCENCEINTENSITYOF8HYDROXYQUINOLINEISLOWA1INORDERTOIMPROVEITSFLUORESCENCEINTENSITY,INTHISPAPERWESYNTHESIZEDFLUORESCENCEREAGENT8HYDROXYQUINOLINEALUMINUMALOX3,ITISAVERYSIGNIFICANTELECTROLUMINSCENTMATERIALANDHASATTRACTEDGREATEDATTENTIONBOTHFROMFUNDAMENTALANDPOTENTIALAPPLICATIONPOINTOFVIEWINTHISEXPERIMENT,THESELFASSEMBLEDBILAYERMEMBRANEALOX3/CYS/AUWASFABRICATEDVIAELECTROSTATICACTIONWITHDLCYSTEINECYSMONOLAYERSONGOLD,SURFACEINORDERTOCHARACTERIZEDITSSTRUCTURE,WEMAKEUSEOFCYCLICVOLTAMMETRYCVINCOMBINATIONWITHXRAYPHOTOELECTRONSPECTRAXPSITWASFOUNDTHATTHESELFASSEMBLEDBILAYERMEMBRANEALOX3/CYS/AUHASGOODSELECTIVITYFORHG2,SOITCANBEAPPLIEDTOTHEDETECTIONOFMERCURYIONMOREOVER,WEFOUNDTHATFLUORESCENTSIGNALOFALOX3/CYS/AUCANBEQUENCHEDBYHG2KEYWORDFLUORESCENCESELFASSEMBLEDBILAYERMEMBRANE8HYDROXYQUINOLINEMERCURYION学士学位论文荧光性双层膜的制备及其在超痕量汞离子检测中的应用摘要A2A0AUSA4A3A5A6DLA8A7A9A10A11A12A13A14A15A16A17A18A19A20A5A21A22A23A248A25A26A27A28HOXA29OHA30CYSA29NH3A31A32A33A34A35A36A37A3A5A12A13A38A39A40A41HOX/CYS/AUA42A14A43A26A44A18A458A25A26A27A28A46A47A48A49A50A51A52A19A53A38A21A22A54A55A56A57A338A25A26A27A28A49ALOX3,A58A59A60A21A22A61A62A39A40A41ALOX3/CYS/AUA19A21A22A22A63A64A65A16A66ALOX3/CYS/AUA21A22A67A68A46A69A70A55A71HOX/CYS/AUA57A42A5A59A60A33ALOX3/CYS/AUA39A40A41A72A70A73A74A75A76A77A24A19A78A79A71A57A33A69A70A55A46A80A81A68A42关键词A21A22A19A11A12A13A39A40A41A198A25A26A27A28,A76A77A24一前言自组装膜SELFASSEMBLEDMONOLAYERS，SAMS是分子通过化学键相互作用自发吸附在固/液或气/固界面，热力学稳定、能G18339G7380G1314的G7389G5219膜，G11013G1122G4439G1867G7389G7143制备、稳定性G3921G1209及G2499G20056G1820G16786G16757G12573特G9869，G5062G7389G3835G18339的文献G6265G17959和G13520G1786013G452G1306是在G2345层膜上G1474G20292G5114G7389荧光基G3254的物质，G1186G13792G2499G1209G17839G15904荧光传感分析的G11752G12362较G4581G452G17837G1039G16213是G11013G1122金G4557其表面的荧光G3254G7143G1147G10995荧光G10481G9793，G1186G13792G19492制G1114自组装膜G6228G7427在荧光分析的G5224用45G452G3926果能G6238G17837G1016G12193分析G6175G8585结合，G2045用自组装G6228G7427（SAM）通过G16855G14422G5225液PH值G7481G6523制SAMS的G5114电G10378G1929，在SAMS表面G19757电吸附上G7389荧光基G3254的荧光物质G13792制成荧光性双层膜，G16825自组装膜在荧光传感G4570G1867G7389G14403G3921的G5224用前G72356G452G11013G1122金表面G7092自G9994G8699化膜，稳定性G3921，G13792G1000与二G11839化合物或G11839G18267G5430成的自组装膜G1319G13007G1867G7389G14403G3921的稳定性，G3252G13792G1209金G11839G1319G13007G1038基G11796的自组装G1319G13007G5460G5460成G1038G11752G12362的G20330选G1319G130077G452半胱氨酸分子G1025G2559G7389G5059基，G7143G1122通过G5430成AUS键吸附G1122金电极表面，G1523G8504G2499G4570半胱氨酸G5353G1849金表面制成G1474G20292电极G4528羟基喹啉作G1038一G12193性能优G5334的螯合剂、G14807G2474剂和金属离子G6363G12046剂，G2499用G1122溶剂G14807G2474、吸光度分析、荧光分析G1257389G4528羟基喹啉G1038荧光G6940G10587较G1314的物质G452G1306G4439与金属离子G4600其是铝离子螯合G1055G2530，荧光G6940G10587G3835G3835G6564G20652912，G7143G1122G6564G13443G1000性质比较稳定1315G452G2528时作G1038一G12193G2559G8706化合物，8羟基喹啉铝是G18337金属离子的G14403G3921G18209G131916，G4557汞离子G1867G7389G14403G3921的选择性，G2499用G1122G18337金属汞离子的G7828测G452学士学位论文G7424论文G12098羟基喹啉和G8707化铝G1038G2419G7021，合成荧光特性G7368G3921的8羟基喹啉铝ALOX3G452在G11752G12362比较8羟基喹啉和8羟基喹啉铝荧光性质的基G11796上，通过G6523制溶液PHG16855G6984其G4396在G5430G5347，G2045用荧光物质与半胱氨酸的G19757电作用G4570其间G6521组装在G2345层膜CYS/AU上，制备荧光物质双层膜ALOX3/CYS/AU，G7389G6940G6245制G1114荧光物质G11464G6521G6521G16314金表面G13792G5353G17227的荧光G10481G9793G452成G2163G3332G16278G4531到G1114ALOX3/CYS/AU自组装膜/溶液界面的强荧光，并采用电化学、荧光光谱，电子能谱和扫描电镜G12573方法表征自组装膜G452G2528时G2045用金属离子会与8羟基喹啉铝发G10995作用G13792G1147G10995荧光G10481G9793的G2419理,G4570自组装膜G6228G7427G5224用G1122荧光分析，构筑一新型G2499再G10995G5347荧光传感和超分子识别G1319G13007G452在实验G1025讨论G1114组装电极的各G12193优化条件确定G1114G1820用DL半胱胺酸溶液浸泡3小时，再用HCL溶液酸化，G7380G2530浸泡G7389一定PH值的荧光物质溶液的方法,制成ALOX3/CYS/AU电极G452实验结果表明用G17837G12193G1474G20292电极测定汞离子G1867G7389较G20652的灵敏度G452二实验部分21实验仪器与试剂211仪器荧光分光光度G16757F4500（日G7424HITACHI）电化学G13520合分析仪BAS100B美国BASS3500扫描电镜（日G7424HITACHI）OX702电子能谱仪（英国OXFORD公司）MILLIQ基G11796型美国MILLIPORE公司G13443化水G13007统212试剂8羟基喹啉铝（自合成，参考17）8羟基喹啉上海曹阳G1025学化工厂DL半胱胺酸上海曹阳G1025学化工厂实验用水G1038MILLIQ基G11796型（美国MILLIPORE公司）G13443化水G13007统G6564供,所用试剂均G1038分析G13443G1209上G452学士学位论文22实验内容221自组装膜的制备2211组装机理通过AUS键作用，在金电极表面组装上DL半胱氨酸，G6521着酸化G2530，再G2045用G19757电吸G5353组装上8羟基喹啉G452反G5224G5347G3926下A821HOX/CYS/AUA83A84A85A86A87A88A89A902212CYS/AU的制备G1820G4570自制的金片表面用石英砂打磨，G9994G2530用AL2O3粉末抛光成镜面G2530，用二次水冲洗，并超声波清洗G1016次，每次5分钟，G6521着在1MOL/LH2SO4G1025用循环伏安法扫描10圈至稳定（三电极G1319G13007AG/AGCL电极G1038参比电极，铂丝电极G1038G4557比电极，金电极G1038工作电极），电位扫描范围G103805V15VG452经G20056处理G2530的金电极用二次水冲洗G2530浸泡在PH57的001MOL/LDL半胱氨酸溶液G10253小时G452G2474出G2530再用二次水冲洗G1209除去电极周围吸附物,G8706气吹干G2530即得CYS/AUG4522213HOX/CYS/AU自组装膜的制备G4570CYS/AU浸泡G112205MOL/L的HCLG1025酸化30MIN，使其表面G5114正电荷，G1122PH10浓度G1038103MOL/L的8羟基喹啉溶液G1025浸泡约1小时，G2474出用二次水冲洗G2530用G8706气吹干，储G4396G1122阴暗处G452SHCH2CHCOOHSCH2CHCOONH2NH2SCH2CHCOOHNH3COOHSCH2CHNH3COOHSCH2CHNH3NOONCYSA91A92HHOXAUAUAUAUPH57PH910学士学位论文2228羟基喹啉铝（8羟基喹啉汞）的合成G45705MMOLALCL36H2OHGCL2加G1849到10MLG1319积比G103811的乙G18267水混合液G1025,再G457015MMOLHOX加G184940ML95乙G18267溶液G1025,微热搅拌至溶解，G1016G12193溶液混合G2530，G112260下边搅拌边滴加5MOL/L氨水至PH910,继续搅拌10MIN，G19757置，抽滤，分别G120995乙G18267和蒸馏水各洗涤3次，60下真空干燥，G1147物G1038黄色晶G1319G452反G5224G5347G3926下A9328A94A95A96A97A98A99A100A101A102A103223ALOX3/CYS/AU的制备8羟基喹啉是荧光G6940G10587G1314的物质，G1306螯合G2530的8羟基喹啉铝荧光G6940G10587G3835G3835G6564G20652，是制备荧光性自组装膜的G14403G3921材G7021G452其组装机理G3926下A1043ALOX3/CYS/AUA105A106A107A108A109A110A111A112224自组装膜荧光光谱测定G4570自组装膜用二次水冲洗并用G8706气吹干G2530，在荧光分光光度G16757SHCH2CHCOOHSCH2CHCOONH2NH2SCH2CHCOOHNH3COOHSCH2CHNH3COOHSCH2CHNH3ALOX3NOALNOONOALNOOPH57AUAUAUAUCYSHPH910A113A114333KNOALNONOAL3NOHH2ONOALNOOHNOALNOOPH9103学士学位论文F4500上测绘校正荧光光谱G452150W的氙灯G1038激发光G9316，G4570膜置G11221CM1CM的石英比色G11411G1025，用固定G7562G16855G6984膜与荧光分光光度G16757的G1849G4568光成50，G1209G1457G16789G7380G3835G19492度G6521G2475荧光G1461G2507G13792干G6212G7380小G452激发和发G4568G10433G13553均G10385NMG452经实验结果表明，HOX/CYS/AU的G7380G1351激发波长G1038EX270NM，G5353G1849AL3G2530，G5430成的ALOX3/CYS/AU的G7380G1351激发波长G1038388NMG452225电化学实验G4570G20056处理过的金片分别浸泡01，005，001，0001MOL/LDL半胱氨酸溶液，在电化学G13520合分析仪BAS100B上G17839G15904电化学实验，分别G1209G16076AU、CYS/AU和HOX/CYS/AUG1038工作电极，置G112205MOL/L的KOHG5225液（G1820通N2约10MIN），G17839G15904循环伏安扫描012V，G1209选择CYS/AU膜的优化条件G452226膜的结构表征用扫描电镜电子能谱仪OX702G4557CYS/AU、HOX/CYS/AU和HGOX2/CYS/AUG17839G15904膜结构表征，电G239920KEV，电G898102NA，在液G8706G1457G6264下测定G452三结果与讨论318羟基喹啉和8羟基喹啉铝溶液溶剂、激发波长和PH的选择311溶剂的选择8羟基喹啉G2499溶G1122G3822G12193G7389机溶剂，考G15397到乙G18267G7424G17535G7092G8614性，G1000G993会G5445G2721到8羟基喹啉的荧光性质，G3252G8504实验G1025选用乙G18267作G10388羟基喹啉的溶剂G452ALOX3G993溶G1122水和乙G18267，G1306能溶G1122三G8707G11014G9931、G1005G18242、环G5061G9931G12573G7389机溶剂G452G6466G7389G1863文献17G6265G17959，ALOX3的荧光G18339子G6940G10587G1039G16213G2475溶剂分子结构和溶剂溶液G12908度的G5445G2721G452在G14471G9879化合物溶剂G1025，ALOX3的荧光G18339子G6940G10587G18129比较G1314G452G13792在G8707G1207G9879溶剂G1025，三G8707G11014G9931ALOX3的荧光G18339子G6940G10587较G3835G3822G6980G7389机溶剂G7481的G20652G452G3252G8504选用三G8707G11014G9931作G1038ALOX3溶剂G452学士学位论文3128羟基喹啉和8羟基喹啉铝溶液的激发波长的选择G2474104MOL/L8羟基喹啉溶液G17839G15904荧光光谱测定，确定其G7380G1351激发波长G452G3926G32824所G12046G5415激发波长G1038270NM时，8羟基喹啉荧光G4804G7380G20652，即荧光性G7380强，G5415激发波长G20652G1122或G1314G1122270NM，荧光G4804G993G7041下G19489，G3252G8504实验G1025采用EX270NMG10388羟基喹啉的激发波长G452G11013文献17G2499G117058羟基喹啉铝的激发波长G1038388NMG452G3252G8504实验G1025分别采用270NM作G10388羟基喹啉的激发波长和388NM作G10388羟基喹啉铝的激发波长G452A1154A116A117A118A119A120A1218A122A123A124A125A126A127A128A129A130A131A132A132A133A1153138羟基喹啉和8羟基喹啉铝溶液PH值的选择考G15397到溶液PH值的G6925G2476G2499能会G4557荧光强度G1147G10995G5445G2721，G3252G8504通过G6523制PH值G1209G14731得G7380G1351荧光G6940果G452实验G1025G18209制G1114G993G2528PH值的浓度G1038104MOL/L8羟基喹啉溶液并测其荧光G452实验结果表明8羟基喹啉乙G18267溶液在G993G2528PH的溶液G1025荧光强度相G5058G993是G3838G3835，8羟基喹啉铝三G8707G11014G9931溶液在酸性下G993稳定，G7143分解，G11013G1122构G5326组装的G16213G8726，G19668G6238荧光物质阴离子化使其G5114上G17139电荷G452G3252G8504在G18209制荧光物质的溶液时G6238PHG16855到910G5050G2503G452在PH910作用下G5114G17139电荷，G7389G2045G1122通过G19757电吸G5353组装G1122CYS/AU表面G452G3252G8504实验G1025G45708羟基喹啉溶液和8羟基喹啉铝溶液PHG16855到910G5050G2503G452350400450500102030405043211EX270NM2EX280NM3EX290NM4EX320NMFLOURSCENCEINTENSITYAUWAVELENGTHNM学士学位论文32自组装膜优化条件的选择实验G1025G17839G15904G1114各G12193实验条件的选择，G7380G2530确定出G7380优的组装条件G452321DL半胱氨酸组装条件的选择3211半胱胺酸在金表面上自组装的电化学表征G6466文献G6265G1795918，CYS/AU膜在NAB4O7NAOHCH3CHOG5225液G1025的循环伏安扫描，伏安G7366G13459G3926G3282所G12046在092VAG/AGCLG5050G2503G7389一明G7186的G993G2499G17882G8699化G4804，表征半胱氨酸G5062组装到金表面上，组装机理G3926下RSHRSADSHE1RSADS3H2ORSO36H5E2A1345BAREAUA135CYS/AUA136A137A138A139A140A141A142A143A144A145A146A134其G1025RG1207表氨基酸G2163能G3254，吸附在金电极上的半胱胺酸G1122正电位的作用下,发G10995G3926G53472的反G5224G452采用05MOL/LKOHG1038G5225液G4570G16076金和CYS/AUG1038工作电极G17839G15904循环伏安扫描，G3926G32826所G12046相比G1122G16076金电极，CYS/AU在077V（AG/AGCL）G5050G2503出G10628一G17836G2419G4804，G16825G4804是吸附G1122金表面G11839的G17836G2419G14085附所G14280G452在105V处G7389一G4804，G2499能是半胱氨酸的双G13870G5353G17227的，表明半胱氨酸G10294固的组装G1122金电极表面G452151005000525201510050005101520AUELECTRODECYS/AUELECTRODECURRENT,UAPOTENTIAL,V学士学位论文A1476BAREAUA148CYS/AUA14905MOL/LKOHA150A151A152A153A154A155A156A157A1473212组装DL半胱氨酸浓度的选择在确定DL半胱氨酸的循环伏安G3282G1025G17836G2419G4804位置G2530，G1038G1114G14731得G7380G1351的自组装G6940果,G4570处理过的金电极G6930G1849G993G2528浓度的半胱氨酸溶液G1025，用G2528一G7693电极分别浸泡01、005、001、0001MOL/L的DL半胱氨酸,G9994G2530在KOHG5225液G1025，G4570G16076金电极和CYS/AU电极作G1038工作电极在012V下分别G17839G15904循环伏安法扫描,发G10628浸泡001MOL/L的CYS/AU出G4804G7380明G7186，G16840明浓度G1038001MOL/LG7380G1038合G17878G4523213组装DL半胱氨酸时间的选择G7424文采用浸泡半胱氨酸3小时G11464至浸泡24小时，组装G993G2528的时间G2530G17839G15904循环伏安扫描,发G10628在001MOL/L装3HG1055G2530组装G6940果相G5058G993G3835,G1000浸泡3小时001MOL/L半胱氨酸溶液所制备的CYS/AU膜G4804G5430和G4804电G8981G1477G1351，G1000基G7424上G17247G1122稳定G452文献19G7389G1863CYS/AU电极界面电容的G11752G12362G6265G17959，G1474G20292G2530的金电极双层界面电容会G19489G1314，G1000G19555着组装时间的G3698长，界面电容G993G7041G1955G4581，G5415吸附时间超过3HG2530，G1474G20292电极的界面电容G1972G1058G993G7041发G10995G2476化，G16840明半胱氨酸分子在3H内G2499G1209在金电极表面吸附组装成稳定的自组装膜，G3252G8504G6117G1216采用3小时的浸泡时间G4520002040608101201000102A158A159A160A158A161A1601BAREAU2CYS/AUCURRENT,MAPOTENTIAL,V学士学位论文3214组装DL半胱氨酸PH值的选择半胱氨酸能G3827通过AUS键结合到金表面，并G1000通过分子自组装G5430成一层G7389G5219的G2345分子G1474G20292层G452其G8505G20600方G12255G5347所G12046AUHSCH2CHNH2COOHA162AUSCH2NH2COOHG11013组装的过G12255G7481G11487，半胱氨酸自组装结构G1025G7094G2559G7389COOH，G2460G2559G7389NH2，G2499G1209通过G6925G2476溶液的PHG7481G6523制CYS/AU表面的荷电G5785G1929，G1427G1122通过G19757电吸G5353组装上荧光物质G452G993G2528PH值的DL半胱氨酸G4557电极G1474G20292G6940果G7389G5468G3835G5445G2721，G6365G10043组装构G5326G16213G8726，DL半胱氨酸G5114正电，8羟基喹啉G2029G19668G5114G17139电荷，G3252G8504G19668G6523制PHG16855G14422其G10378G5589，G6466文献G6265G17959，PH57时，DL半胱氨酸G2588SCH2CHNH3COOHG5430G5347，G7389G2045G1122组装G452实验过G12255G1025G1075G18209制G1114G993G2528PH值G2528浓度的DL半胱氨酸，G17839G15904G4557比实验G452结果G1075G16789明G1114在PHG103857G5050G2503组装G6940果G7380G3921，G3252G8504选用PH57的DL半胱氨酸G17839G15904组装G452322CYS/AU电极酸化时间和浓度的选择经过反G3809实验G11752G12362表明，浸泡05MOL/LHCL能使荧光物质与DL半胱氨酸的G19757电作用较强，组装的膜荧光强度G7380G3835，G3252G8504选择05MOL/LHCL作G1038酸化条件G4523238羟基喹琳荧光物质及其金属螯合物的组装3231确定荧光物质的激发波长酸化G2530的CYS/AU浸泡在103MOL/L，PH10的8羟基喹啉和8羟基喹啉铝溶液G1025，组装G2530的HOX/CYS/AU和ALOX3/CYS/AU双层膜分别采用G993G2528的激发波长G17839G15904荧光测定，G3926G32827A所G12046G5415激发波长G1038270NM时，组装HOX/CYS/AU荧光G6940果G7380G3921G452G19555着激发波长的G3698G3835，荧光G4804G2376G993G7041下G19489，G3252G8504，HOX/CYS/AU双层膜的荧光光谱测定时，选用EX270NMG1038G7380G1351的激发波长,G3926G32827B所G12046，388NMG1038ALOX3/CYS/AUG7380G1351波长G452学士学位论文A1637A164A165A166A167A168A169A170A171HOX/CYS/AUAALOX3/CYS/AUBA172A173A173A174A1633232浸泡时间与荧光物质浓度的选择G4570CYS/AU浸泡在HOX溶液G1025G14521时间过长会G4560G14280金表面的吸附G18339过G3835，使膜G993G3827均G2260，G10294固，经反G3809实验G16789明，G4570CYS/AU电极浸泡在荧光溶液G10251小时组装G6940果G3921,G1000用时G11713G452G3252G8504采用1小时的浸泡时间G452分子的自组装是G2345分子G7389G5219G6502G2027，G14521组装物质浓度G3838G20652，会使组装在电极上的物质G7446G1093G7092G12468G13792G5445G2721组装G6940果G727G14521浓度G3838G1314，组装G2530荧光强度G993G20652或G13785测G993出荧光G452G54158羟基喹啉浓度G3835G1122102MOL/L时，浓度G3838浓，用G1122组装双层膜G2530测G993出荧光，G5415浓度G1314G1122104时，G11013G1122浓度G3838G1314，组装G2530的HOX/CYS/AU荧光强度G993G20652G452G13520合考G15397，实验采用0001MOL/L的8羟基喹啉和8羟基喹啉铝溶液G7481组装CYS/AU电极G4523233PH值的选择G6365G10043组装G16786G16757G16213G8726，8羟基喹啉和8羟基喹啉铝G20047G5114G7389G17139电荷，G6177能与G5114G7389正电荷的CYS/AU电极通过G19757电吸G5353组装成双层膜G452G3252G8504G16213通过G6523制PH值G7481G16855G144228羟基喹啉和8羟基喹啉铝的G4396在G5430G5347G452实验结果表明，在PH910条件下组装双层膜的荧光强度较G3921，G3252G8504选用PH910的8羟基喹啉和8羟基喹啉铝溶液用G1122自组装双层膜G452G1186G1209上的讨论得G11705HOX/CYS/AU和ALOX3/CYS/AU自组装的G7380G1351条件是G4570自制金4004204404604805005200300600900120015001800A175A176A17743211EX270NM2EX280NM3EX290NM4EX300NMFLOURSCENCEINTENSITYWAVELENGTHNM4404604805005205405605806000100200300400500600700800A178A179A180A178A181A180A182A183A184A185A186A186A187A188A178A189A180A182A183A184A185A190A186A187A188A178A185A180A182A183A184A191A181A192A187A188321FLUORESCENTINTENSITYAUWAVELENGTHNM学士学位论文电极在001MOL/LPH57的DL半胱氨酸G1025浸泡3H，用二次水冲洗，G8706气吹干G2530，G112205MOL/L的HCL酸化30MIN,，G2474出G2530用二次水冲洗，G8706气吹干，再浸泡在PH910浓度G10380001MOL/L8羟基喹啉乙G18267溶液和8羟基喹啉铝三G8707G11014G9931溶液G10251HG45233自组装双层膜的荧光光谱G6365G1209上优化条件制备组装双层膜G2530，G4570G16076金，CYS/AU和HOX/CYS/AU和ALOX3/CYS/AU分别置G1122荧光G8756G1025，在F4500荧光光度G16757上测其的荧光谱G3282G452G3926G32828、9所G12046，G16076金和CYS/AU电极均G8821G7389出G10628荧光G4804，组装8羟基喹啉的HOX/CYS/AUG1177出G10628微G5381的荧光G4804，G13792用8羟基喹啉铝组装的ALOX3/CYS/AUG1122522NMG5050G2503G7186G12046出强的荧光G4804G452G1186G13792G16840明G1114荧光物质G5062成G2163组装在金电极表面G452A1938AUA194CYS/AUA194HOX/CYS/AUA195A196A197A198A199A199A200A193400440480010020030040050060070080090010003211BAREAU2CYS/AU3HOX/CYS/AURELATIVEFLOURESCENCEINTENSITYAUWAVELENGTHNM学士学位论文A2019AUA202CYS/AUA202ALOX3/CYS/AUA203A204A205A206A207A207A208A20134自组装膜的表征341电极荧光光谱表征G4570G16076AU、CYS/AU和HOX/CYS/AU分别测定其荧光光谱，G3926G32828、9所G12046，G16076AU和CYS/AU均G8821G7389出G10628荧光G4804，G2494G7389HOX/CYS/AU和ALOX3/CYS/AUG6177分别在450NM和522NM处G7389荧光G4804出G10628，表明荧光物质G5062经组装到CYS/AU上G452G32829G1025ALOX3/CYS/AUG1122EM522NMG7389出G10628荧光G4804，G137928羟基喹啉铝三G8707G11014G9931溶液出G4804位置G1038511NMG452G17837G2499能是G11013G11228羟基喹啉铝分子在自组装膜上和在溶液G1025所处的微环G3671G993G2528，G13792使分子间作用力G993G2528所G14280G452342膜的结构表征G328210A、B、C是CYS/AU和ALOX3/CYS/AU、ALOX3/CYS/AU浸泡汞离子前G2530的扫描电镜G10043片，G1186G3282G1025G2499G1209G11487出，相比G1122CYS/AUG2345层膜的表面，ALOX3/CYS/AU双层膜表面G7389明G7186的G993G2528G452G4570ALOX3/CYS/AU浸泡G1114汞离子G2530，膜表面G7389固G1319G20075G12902出G10628G328210CG452相G4557G5224的浸泡汞离子前G2530的ALOX3/CYS/AU膜的能谱G3282G1075G16789明G3926G8504，G3926G328211B所G12046，G2499G1209发G10628浸泡汞离子G2530的ALOX3/CYS/AU能谱G3282G1025G7389HGG1815G13044出G10628，G6524测是汞离子与ALOX3/CYS/AU作用G13792结合在膜表面上G452440480520560010020030040050060070080090010003211BAREAU2CYS/AU3ALOX3/CYS/AURELATIVEFLOURESCENCEINTENSITYAUWAVELENGTHNM学士学位论文ABCA20910CYS/AUA210A211A212A213A209ALOX3/CYS/AUA214A215HG2A216BA217A218CA219A210A211A212A213A209ABA22011ALOX3/CYS/AUA221A222A223HG2A224AA225A226BA227A228A229A230A231A220358羟基喹啉铝、8羟基喹啉溶液及自组装膜稳定性能的比较351溶液荧光强度的比较实验过G12255G1025，分别G4557G2528一浓度下的8羟基喹啉铝、8羟基喹啉溶液及其自组装分别G17839G15904荧光光谱测定，G1186G32829G2499G1209G11487出，表明8羟基喹啉铝溶液和自组装双层膜荧光G6940果较8羟基喹啉G3921G452金属离子G7424G17535G8821G7389荧光特性，G1306G11013G1122G4439的G4396在，G2499使一G1135G2419G7481G993发G10995荧光的物质发G10995荧光或使G5381荧光物质成G1038强荧光物质，G17837G12193作用G12228G1038金属离子微G6212下的G18209位G1319发光G452G18039G1135G1867G7389稳定的G1227G5589，G13792G1000电子构型属G1122G1817G9397、半G1817G9397或G1852空的金属离子，G4439G1216所G5430成的8羟基喹啉螯合物G1867G7389强的荧光特性G452AL3是一G12193稳定的G8699化G5589，其G1227电子G17724G17959G1852空，8羟基喹啉铝的G18209位G1319OX在A13的微G6212下，G2475激发发G10995跃迁，G9994G2530发G4568强的荧光G452352稳定性的比较实验结果表明8羟基喹啉G7143G2475潮G2476黄，固G1319及溶液露置G1122空气G1025G2476黄G17839G13792G2476学士学位论文黑，在空气G1025及G20652温下是G993稳定的，并G1000组装HOX/CYS/AU双层膜稳定性G5058，G6930置一周G2530再G17839G15904测定，测G993出荧光G4528羟基喹啉与铝离子的络合作用G5468强，G1306是溶液G2029是G993稳定的，其溶液G19757置一周G2530G1427会G11013浅黄色G2476G1038褐色至黑色G452在实验G1025，G18129使用新鲜G18209制的8羟基喹啉铝溶液G452在G4557其溶液的稳定性G11752G12362的G2528时，G17836G17839G15904G1114G1209下的实验L测G18339组装G2530的ALOX3/CYS/AU双层膜的荧光强度，G6930置五周G2530（避光G1457G4396），再测G18339其荧光强度，发G10628G1016G13785的荧光强度并G8821G7389发G10995G2476化G452膜的稳定性较G3921G452G13520合荧光强度和稳定性能G1016方面G7481考G15397，在选用双层膜G4557汞离子G17839G15904测定实验G1025，采用8羟基喹啉铝G7481G1474G20292CYS/AUG1209G8504自组装双层膜G7481实G10628G4557汞离子较G20652灵敏度和选择性的测定G45236HG2的荧光传感分析361汞离子的荧光传感的分析特性实验过G12255G1025发G10628HG2的作用会G4560G142808羟基喹啉铝荧光物质的荧光强度G1955G5381，即发G10995荧光G10481G9793，G2499用G1122HG2传感分析G452基G1122荧光G10481G9793的G2419理，实验G1025G4570组装电极G5224用G1122HG2的荧光传感分析G452G1209I0/IG4557汞离子浓度作G3282，G3926G328212（A）所G12046G19555着HG2的G3698加，ALOX3/CYS/AU自组装膜荧光强度G993G7041下G19489G452G1038G1114G16840明测定G1314浓度汞离子的准确性,G17839G15904G1114空白实验G452结果G3926G328212（B）所G12046二次水G1025汞离子浓度G1314,在实验G1025G993G1147G10995干G6212G452G328212G10481G9793实验与空白实验比较G3282G54304204504805105405706000200400600800100012001400160018002000A232A233A234A235A236A237A238A239A240A241A242A243A244A245A246A239A240A241A242A243A244A245A247A239A240A241A242A243A244A245A248RELATIVEFLOURESCENCEINTENSITYAUWAVELENGTHNM3904204504805105405706000200400600800100012001400160018002000A249A250A251A252A253A254A255A1A0A2A3A4A5A6A7A8A9A10A11A12A13A14