增生性玻璃体视网膜病变组织中的单核细胞趋化因子1的意义.doc

-1 :;-1;T-; :PVR.-1MCP-1.MCP-1.BPVRPVRC/DMCP-1CD68CD3.MCP-1BPVRMCP-1PVR.CD68CD3T-PVR.MCP-1PVR. Keywords:vitreoretinopathyproliferative;monocytechemoatlractantprotein-1;macrophages;t-lymphocytes;immunohistochemistry Abstract:AIMToinvestigatetherelationshipbetweenthepresenceofMCP-1andmacrophagesandT-lymphocytesinspecimensobtainedfromretinaldetachmentandPVReyes. METHODSSpecimenswereobtainedduringretinalsurgeryincludingcellularcomponentsofsubretinalfluidsinrhegmatogenousretinaldetachmentRDfluidscollectionsduringvitrectomyforPVRgradeBandepiretinalmem-branesfromPVRgradeCorD.Immunohistochemicaldetec-tionwasperformedtoexaminethedistributionofMCP-1andthepresenceofCD68orCD3positivecellsinthesespecimens.RESULTSTheexpressionofMCP-1wasnegativeorveryweakincellsofsubretinalfluidfromRD.AsafunctionoftheincreaseofPVRgradeMCP-1expressionbecamestrongerwhiletherateofCD68+/CD3+decreased.CONCLUSIONMCP-1maymodulatethewoundhealingaf-terretinaldetachmentandtheformationofepiretinalmem-branesinPVR. 0 proliferativevitreo-retinopathyPVRPVR12.PVRPVRretinalpigmentepitheliumRPE3.:TNF-IL-1PDGF-TGF-4.-1MCP-15RPEMCP-16PVR7.MCP-1PVRPVRMCP-1. 1 1.1 1997-09/2000-0356.121500r・min -15min40g・L-130min4. 44PVRPVR1983.20PVRB;24PVRC1410.40g・L-130min4.5m.CD3TCD68CD3CD68MCP-1PharMingen. 1.2 :7.5mL・L-1H2O2-PBS3730min.3730min.PBS3IgG3730min.ABC3730min.HE4010RPEMCP-1.MCP-110%10%69%+70%.CD68CD3401005CD68/CD3CD68/CD3.:xs2t. 2 12RPEMCP-1.PVRB20RPE.:MCP-17CD68/CD34.51.7;MCP-111CD68/CD32.71.3;MCP-12CD68/CD32.51.3.PVRC10Fig1MCP-15CD68/CD31.30.65CD68/CD31.10.5;PVRD14CFig2.MCP-14CD68/CD30.80.4MCP-110CD68/CD30.40.3.PVRBCDMCP-1P<0.01CMCP-1DP<0.05.MCP-1CD68/CD3P<0.01. 3 PVRMCP-1.MCP-1;PVRBMCP-1;PVRMCP-1.MCP-1CD68/CD3PVRBCPVRDT. MCP-1.MCP-1PVR.RPEMCP-1IL-1TNF-6. PVR.PVRT-3.89.IL-1TNF-IL-6IL-810.MCP-1.PVR. MCP-1PVRMCP-1.RPEMCP-1;PVRMCP-1.RPE.RPEMCP-1RPE. CD68/CD3.PVRCD68/CD3PVRT-PVR.11MCP-1.MCP-1PVRT-. : 1KirchhofBSorgenteN.Pathogenesisofproliferativevitreo-retinopathyJ.DevOphthalmol1989;161:1-53. 2WellerMWiedemannPHeimannK.Prolifertivevitreo-retinopathyisitanythingmorethanwoundhealingatthewrongplace?J.IntOphthalmol1990;142:105-117. 3BaudouinCFredj-ReygrobelletDGordonWCVeckeneerMJonesSE.ImmunohistologicstudyofepiretinalmembranesinproliferativevitreoretinopathyJ.AmJOphthalmol1990;1104:593-598. 4LimbGAEarleyOJonesSE.ExpressionofmRNAcodingforTNF-IL-1andIL-6bycellsinfiltratingretinalmembranesJ.GraefesArchClinExpOphthalmol1994;2327:646-651. 5VanDammeJ.Interleukin-8andrelatedchemotacticcytokinesA.In:ThomsonAed.ThecytokinehandbookM.Lon-don:AcademicPress1994:1185-208. 6ElnerVMBurnstineMAStrieterRMKunkelSLElnerSG.Cell-associatedhumanretinalpigmentinterleukin-8andmono-cytechemotacticprotein-1:Immunochemicalandin-situhy-bridizationanalysesJ.ExpEyeRes1997;658:781-789. 7AbuEIAsrarAMVanDammeJPutWVeckeneerMDra-landsLBilliauAMissottenL.Monocytechemotacticpro-tein-1inproliferativevitreoretinaldisordersJ.AmJOph-thalmol1997;1235:599-606. 8HuiYNSorgenteNRyanSJ.PosteriorvitreousseparationandretinaldetachmentinducedbymacrophagesJ.GraefesArchClinExpOphthalmol1987;2253:279-284. 9BakunowiczLASulkowskiSMoniuszkoT.Comparativestud-iesofmorphologicalchangesandinterleukinconcentrationinsubretinalfluidofpatientswithretinaldetachmentJ.Oph-thalmologica1999;2131:25-29. 10HuiYNShiYNZhangXHYangKYuCH.Proinflamma-torycytokinesinMacrophagesinducedproliferativevitreo-retinopathymodleofrabbitJ.ZhonghuaYankeZazhiChinJOphthalmol1999;352:140-143. 11EngelhardtEToksoyAGoebelerMDebusSBrockerEBGilliz