文献汇报.ppt

Mesenchymalstemcell-derivedextracellularvesiclesandretinalischemia-reperfusion,汇报人：张永杰导师：田雪松,BijiMathewUniversityofIllinoisatChicago|DepartmentofAnesthesiology(Chicago)PhD,Microbiology,StevenRothOphthalmologyandVisualScience,CollegeofMedicine,USA|DepartmentofAnesthesia6(5):526-539,Extracellularvesicles,CellularandMolecularLifeSciences2011;68(16):2667-88.,Fig.1.CharacterizationofMSCderivedEVs.,Fig.2.EndocytosisofMSC-EVsbyR28cells,Fig.3.Heparansulfateproteoglycans(HSPGs),butnotintegrins,areinvolvedinendocytosisofMSC-EVsbyR28cells,RGD:精氨酸-甘氨酸-天冬氨酸肽HSPG:硫酸肝素蛋白聚糖,Fig.4.Heparansulfateproteoglycans(HSPGs),butnotintegrins,areinvolvedinendocytosisofMSC-EvsbyR28cells,MBCD:甲基-环糊精,Caveolin-1：小窝蛋白1Clathrin：网格蛋白,Fig.5.InvolvementofthecaveolarpathwayinMSC-EVendocytosisbyR28cells,小总结：,1.骨髓来源间充质干细胞分泌的细胞外囊泡大多数是外泌体2.细胞外囊泡可以被R28细胞以剂量依赖和温度依赖性所饱和内吞，其机制和细胞膜上的小窝蛋白1、外囊泡上的硫酸肝素蛋白聚糖（HSPG）相关3.细胞外囊泡可对氧糖剥夺实验（OGD）后的R28细胞有保护作用,那么细胞外囊泡是否能对视网膜缺血再灌注损伤的动物起到保护作用呢？,Fig.6.MSC-EVadministrationfollowingretinalischemiainvivoattenuatesischemicdamage,Fig.7.MSC-EVsattenuatedischemia-inducedapoptosisinischemicretinaeinvivo.,Fig.8.MSC-EVsattenuatedneuro-inflammationandcaspase3activationafterretinalischemiainvivo,Fig.9.Invivoliveimagingofintra-vitreallyinjectedgreenfluorescentMSC-EVs,Fig.10.UptakeanddistributionofMSC-EVsbynormalandischemicretinaeinvivo,Fig.10.UptakeanddistributionofMSC-EVsbynormalandischemicretinaeinvivo,Fig.11.HighmagnificationconfocalimagingofretinalflatmountsshowsthatretinalneuronsandretinalganglioncellstakeupMSC-EVs,andthatischemiaincreasesuptake.,T3：-微管蛋白3,Conclusion,1.MSC-EVsareendocytosedbyretinalcellsinareceptor-mediated,dose-dependentandsaturablemanner.2.TheendocytosedEVscanprotectretinalcel