NEB酶切buffer.docx

home technical reference restriction endonucleases nebuffer activity chartnebuffer activity chart for restriction enzymesfor your convenience, new england biolabs offers a simple 4 buffer system. a color-coded 10x nebuffer is supplied with every restriction endonuclease, ensuring 100% activity. over 170 restriction enzymes exhibit 100% activity in nebuffer 4, resulting in increased efficiency, flexibility and ease of use especially when performing double digests.to help select the best conditions for double digests, this chart shows the optimal (supplied) nebuffer and approximate activity in the four standard nebuffers and nebuffer ecori for each enzyme. if bsa is supplied with an enzyme, it is included in all nebuffer activity reactions. in addition, the table shows recommended reaction temperature, heat inactivation temperature, recommended diluent buffer and whether the enzyme is time-saver qualified (i.e., cleaves substrate in 5 minutes under recommended conditions). note: the values listed in this table are approximate. they were obtained using each enzymes specific unit assay substrate dna. please check out other technical reference information related to restriction enzymes: double digestion | heat inactivation | activity at 37c | diluent buffers | time-saver enzymes | high fidelity (hf) restriction enzymesclick for the chart legend, icon description a | b | c | d | e | f | h | i | k | l | m | n | p | r | s | t | x | z | enzymesupplied nebuffer% activity in nebuffersheatinac.cincu.temp.cdilu-ent1234ecoriaatiinebuffer 405050100256537aacc65inebuffer 3 + bsa107510025506537aaccinebuffer 450501010058037aaciinebuffer 32550100501006537aaclinebuffer 4 + bsa101001005no37bacuinebuffer 4 + sam50100501001006537bafeinebuffer 42550251001006537bafliinebuffer 4 + bsa501002510006537aafliiinebuffer 3 + bsa2575100501008037bageinebuffer 1100501075506537cagei-hfnebuffer 4 + bsa1005010100256537aagei-hf re-mix-nrnrnrnr-6537-ahdinebuffer 4 + bsa25750100106537aaleinebuffer 410251010006537aaluinebuffer 4100100751001006537balwinebuffer 4501001010056537aalwninebuffer 410100100100106537aapainebuffer 4 + bsa2550010056525aapalinebuffer 4 + bsa100100101005no37aapekinebuffer 3257510050100no75bapoinebuffer 3 + bsa1075100751008050aascinebuffer 40101010056537aasci re-mix-nrnrnrnr-6537-aseinebuffer 3nr75100 ddnr506537basisinebuffer 4 + bsa5010010010008037bavainebuffer 4107510100508037aavaiinebuffer 4507510100506537aavriinebuffer 410010050100508037benzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entbaeginebuffer 11007510501006537abaeinebuffer 4 + bsa + sam5010050100406525abamhinebuffer 3 + bsa75100100 dd100100no37abamhi-hfnebuffer 4100501010025no37abaninebuffer 4 + bsa5010050100256537abaniinebuffer 410010501001008037abbsinebuffer 21001002575506537bbbvcinebuffer 450100101001008037bbbvinebuffer 210010025751006537bbccinebuffer 1 + bsa100501050156537abceainebuffer 3 + bsa1001001001001006537abcginebuffer 3 + samnrnr100 ddnr506537abcivinebuffer 4100500100256537cbclinebuffer 350100100 dd75100no50abcodinebuffer 45075751001006537bbfainebuffer 475501010008037bbfuainebuffer 3075100101006550bbfucinebuffer 4 + bsa1005025100158037bbglinebuffer 35075100501006537bbgliinebuffer 3107510010100no37ablpinebuffer 4501001010050no37abmgbinebuffer 3 + bsa02510010506537cbmrinebuffer 27510075100506537bbmtinebuffer 22510025501006537bbmti-hfnebuffer 4 + bsa5010010100-6537bbpminebuffer 3 + bsa751001001001006537bbpu10inebuffer 31025100251008037bbpueinebuffer 4 + sam50100101001006537bbsaainebuffer 4100100100100100no37cbsabinebuffer 450100751001008060bbsahinebuffer 4 + bsa501001001001008037absainebuffer 4 + bsa75751001001006537bbsai-hfnebuffer 4 + bsa252510100106537bbsajinebuffer 41001001001001008060absawinebuffer 4 + bsa5010050100408060absaxinebuffer 4751001010015no37bbserinebuffer 4100100751001006537abseyinebuffer 31050100501006537bbsginebuffer 4 + sam507550100206537bbsieinebuffer 4 + bsa501001010058060absihkainebuffer 4 + bsa5010010010025no65bbsiwinebuffer 3100100100251008055abslinebuffer 31050100751008055absmainebuffer 4501001001001008055bbsmbinebuffer 3751001001001008055bbsmfinebuffer 4 + bsa255050100158065absminebuffer 4751007510058065absobinebuffer 21010010050100no37absp1286inebuffer 4 + bsa252525100106537abspcninebuffer 4 + bsa + sam1007510100158025abspdinebuffer 42575501001006537abspeinebuffer 301010001008037bbsphinebuffer 4101005010056537abspminebuffer 3nrnr100nr1006537bbspqinebuffer 410501001001008050bbsrbinebuffer 4501001001001008037absrdinebuffer 2 + bsa501005075508065absrfinebuffer 410100100100100no37cbsrginebuffer 2 + bsa2510010100508037absrinebuffer 3050100101008065bbsshiinebuffer 3100100100 dd1001008050bbsskinebuffer 3 + bsa050100501008060absssinebuffer 3 + bsa050100 dd101008037bbstapinebuffer 4 + bsa251001001001008060abstbinebuffer 475502510050no65absteiinebuffer 3 + bsa507510075100no60absteii-hfnebuffer 4 + bsa5075100100-no37absteii-hf re-mix-nrnrnrnr-no37-bstninebuffer 2 + bsa101001007550no60abstuinebuffer 410010050100100no60abstxinebuffer 3050100251006537bbstyinebuffer 25010075100508060abstz17inebuffer 4nrnr100100100no37bbsu36inebuffer 3 + bsa0251000158037abtginebuffer 3 + bsa25501001001008037bbtgzinebuffer 4 + bsa10250100508060abtscinebuffer 4501005010025no50bbtsinebuffer 4 + bsa1005050100258055abtsimutinebuffer 4 + bsa1005010100-8055aenzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entcac8inebuffer 45075100100506537bclainebuffer 4 + bsa105050100506537acspcinebuffer 4 + sam1010010100206537acviaiinebuffer 4 + bsa752510100256525ccviki-1nebuffer 41050251001008037acviqinebuffer 3 + bsa75100100750no25benzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entddeinebuffer 375100100751006537adpninebuffer 4100100751001008037bdpniinebuffer dpniinrnr100nr1006537bdrainebuffer 47575501001006537adraiiinebuffer 3 + bsa1007510025506537bdraiii-hfnebuffer 40501010050no37bdrdinebuffer 4 + bsa2550101001006537aenzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-enteaeinebuffer 4100100501001008037aeaginebuffer 31025100101006537ceagi-hfnebuffer 4251001001001006537bearinebuffer 4100100501001006537aeciinebuffer 41005050100-6537aeco53kinebuffer 41005025100106537aeconinebuffer 4100100751001006537aecoo109inebuffer 4 + bsa10010075100156537aecop15inebuffer 3 + bsa + atp75100100100506537aecorinebuffer ecori1001001001001006537cecori-hf re-mix-nrnrnrnr-6537-ecori-hfnebuffer 410100010056537cecorvnebuffer 3 + bsa507510050508037aecorv-hf re-mix-nrnrnrnr-6537-ecorv-hfnebuffer 4251001001001006537benzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entfatinebuffer 21010050501006555afauinebuffer 41005010100506555afnu4hinebuffer 4102525100106537afokinebuffer 410010075100256537afseinebuffer 4 + bsa100750100506537bfspeinebuffer 4 + bsanrnrnr100-6537bfspinebuffer 4107510100506537cenzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-enthaeiinebuffer 4 + bsa7510050100258037ahaeiiinebuffer 450100251001008037ahgainebuffer 11007550100256537ahhainebuffer 4 + bsa751001001001006537ahinciinebuffer 3 + bsa751001001001006537bhindiiinebuffer 2501001050256537bhindiii-hfnebuffer 41010010100108037bhinfinebuffer 475100751001008037ahinp1inebuffer 41001001001001006537ahpainebuffer 4252510100100no37ahpaiinebuffer 1100501050156537ahphinebuffer 4nr750100106537bhpy166iinebuffer 410010050100206537chpy188inebuffer 4502510100506537ahpy188iiinebuffer 4 + bsa1001001010056537ahpy99inebuffer 4 + bsa10100100256537ahpyavnebuffer 4 + bsa10010025100506537bhpych4iiinebuffer 41005025100208037ahpych4ivnebuffer 1100251025156537ahpych4vnebuffer 4505025100506537benzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-enti-ceuinebuffer 4 + bsa10100100-6537bi-sceinebuffer i-scei + bsa10505050-6537benzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entkasinebuffer 4 + bsa2510001001006537bkpninebuffer 1 + bsa1007505025no37akpni-hfnebuffer 41002501000no37akpni-hf re-mix-nrnrnrnr-no37-enzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entlpnpinebuffer 4 + bsanrnrnr100-6537benzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entmboinebuffer 4751001001001006537amboiinebuffer 4100100501001006537cmfeinebuffer 475501010056537amfei-hf re-mix-nrnrnrnr-6537-mfei-hfnebuffer 475501010006537amlucinebuffer 4100101010008037amluinebuffer 32575100501006537amlyinebuffer 4 + bsa505025100256537ammeinebuffer 4 + samnrnrnr100 dd508037bmnlinebuffer 4 + bsa7510050100506537bmscinebuffer 47575751001006537bmseinebuffer 4 + bsa7510075100506537amslinebuffer 410010025100106537amspa1inebuffer 4 + bsa5010050100506537bmspinebuffer 4751005010050no37amspjinebuffer 4 + bsanrnrnr100-6537bmwoinebuffer 3107510075100no60benzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entnaeinebuffer 41007510100256537anarinebuffer 41007575100506537anb.bbvcinebuffer 25010010100-8037anb.bsminebuffer 32510010025-8065anb.bsrdinebuffer 2101002550-8065anb.btsinebuffer 4 + bsa7510075100-8037anciinebuffer 4100251010025no37ancoinebuffer 31001001001001006537ancoi-hf re-mix-nrnrnrnr-8037-ncoi-hfnebuffer 450100101001008037bndeinebuffer 475100751001006537angomivnebuffer 4100501010008037anheinebuffer 2 + bsa10010010100156537cnhei-hf re-mix-nrnrnrnr-8037-nhei-hfnebuffer 4 + bsa10010010008037cnlaiiinebuffer 4 + bsa25252510056537bnlaivnebuffer 4 + bsa1010101001006537bnmeaiiinebuffer 4 + sam1010010008037bnotinebuffer 3 + bsa050100251006537cnoti-hf re-mix-nrnrnrnr-6537-noti-hfnebuffer 4 + bsa2510025100506537anruinebuffer 3010100101006537ansiinebuffer 31075100251008037anspinebuffer 2 + bsa100100010056537ant.alwinebuffer 2251002550-8037ant.bbvcinebuffer 45010010100-8037ant.bsmainebuffer 41005010100-6537ant.bspqinebuffer 3107510025-8050bnt.bstnbinebuffer 301010010-8055ant.cvipiinebuffer 42510050100-6537aenzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entpacinebuffer 1 + bsa100751010056537apaci re-mix-nrnrnrnr-6537-paer7inebuffer 4251001010050no37apciinebuffer 3 + bsa50751005008037bpflfinebuffer 4 + bsa0252510006537apflminebuffer 3 + bsa0100100501006537aphoinebuffer 3505010075100no75api-pspinebuffer pi-pspi + bsa0101010-no65bpi-sceinebuffer pi-scei + bsa0000-6537bpleinebuffer 410100100100506537apmeinebuffer 4 + bsa0501010056537apmlinebuffer 1 + bsa10075075156537appuminebuffer 4025010010no37bpshainebuffer 4 + bsa5050010056537apsiinebuffer 41010010100106537bpspginebuffer 47510050100100no75apspominebuffer 4252510100156537bpspxinebuffer 4010010100508037bpstinebuffer 3 + bsa757510050508037cpsti-hfnebuffer 4107550100258037cpvuinebuffer 3 + bsa107510010100no37bpvui-hfnebuffer 4 + bsa25100100100100no37bpvuiinebuffer 2100100100100100no37bpvuii-hfnebuffer 4025010008037benzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entrsainebuffer 410010050100156537arsriinebuffer 4257510100256537cenzyme topsupplied nebufferb1b2b3b4ecoriheatinac.incu.temp.dilu-entsacinebuffer 1 + bsa1005010100156537asaci-hfnebuffer 4 + bsa1005010100156537asaciinebuffer 42575101001006537asalinebuffer 3 + bsa001000506537asali-hf re-mix-nrnrnrnr-8037-sali-hfnebuffer 4101001001001006537asapinebuffer 475500100506537bsau3ainebuffer 1 + bsa100501010056537asau96inebuffer 450100100100508037asbfinebuffer 475500100256537asbfi-hfnebuffer 45025010006537bscainebuffer 3nrnr100 ddnr508037ascai-hf re-mix-nrnrnrnr-6537-scai-hfnebuffer 410010010100156537bscrfinebuffer 41001001001001006537csexainebuffer 4 + bsa1007550100506537asfaninebuffer 307510025506537bsfcinebuffer 4 + bsa755010100256537bsfiinebuffer 4 + bsa01001010015no50csfoinebuffer 42510050100100no37bsgrainebuffer 41005010100506537asmainebuffer 400010006525asmlinebuffer 4 + bsa25752510050no55asnabinebuffer 4 + bsa25502510058037aspeinebuffer 4 + bsa75100251005