同步辐射元素成像技术课件

1、An electron in the inner shell (e.g. K shell) is ejected from the atom by an external primary excitation x-ray, creating a vacancy. An electron from outer shell jumps in to fill the vacancy. In the process, it emits a characteristic x-ray unique to this element and in turn, produces a vacancy in the

2、 outer shell. RF Primary X-ray1 mmSchematic drawing of anatomical structures of an adult hermaphrodite C. elegans Elemental distribution in C. elegans which was fed by E. coli OP50+ PBS (A), E. coli OP50 + nano Cu (B), and E. coli OP50 +Cu2+ (C). JAAS, 2008, 23, 112111243.55.0 m at BL-4A in, KEKXRF

3、microprobe at beamline 2-ID-E of the Advanced Photon SourceFollowing monochromation the X-ray beam was focused to a spot of approximately 270 nm diameter using a Fresnel zone-plate objective. Fluorescence spectra were recorded at 150 nm intervals as the specimen was raster scanned through the focus

4、of the X-ray beam to obtain 2D projection images. The flux transmitted by the sample was monitored using both an ion chamber and a configured detector. A rotation series was obtained by acquiring such images with the specimen oriented at 24 different angles with respect to a vertical rotation axis.P

5、teris vittatametal monocapillariesBL 9-3 at SSRLGe detectorAs(glutathione)3 11869.8 eVArsenite 11871.4 eVArsenate 11874.8 eVBiological SamplesSR45 Si(Li) detectorAmplifier MCA Computer Slit Ionization chamber Sample moving directionIEF separation SIGOUTReconstruction SRXRF analysis Protein extractio

6、n 05001000150020000100200300400CountsChannelDetection of metalloproteins with SRXRF after electrophoresisGao Y, Anal. Chem. Acta, 2003, 485(1): 131-137Gao Y, Analyst, 2002, 127, 1400-1404 Gao Y, JAAS, 2007, 22, 856866Li LN, J Toxicol Env Health, A, 2008, 71: 12661269ACS CHEM BIOL,ACS CHEM BIOL,2010,

7、2010,5,577-587IIIThree-dimensional protein spots of liver sample contained calcium, iron and zinc, which highlighted with a circle.Talanta,2010,82,1052-1056E E0 010 10 E E0 0+ +10-5010-50E E0 0+50-1000 +50-1000 Protein map (a) and Cr map (b) for Cr compounds binding to albumin-depleted bovine serum

8、(BS) proteins. The main protein bands at 65 and 130 kDa correspond to monomeric and dimeric forms of BSA, respectively, total protein content in each lane is 10 g. Lane 1: BS. Lane 4: GSH (5.0 mM), Lane 2,3: Cr(III), Cr(IV) (1.0 mM)+BS. Lane 5,6:Cr(IV), Cr(III)-(1.0 mM) + GSH (5.0 mM). Lane 7: Cr(II

9、I)-propionate, 1.0 mM). All Cr compounds were incubated with serum proteins (1.0 mg protein per mL in 20 mM HEPES buffer, pH 7.4) for 2 h at 310 K prior to the separation by SDS-PAGE.ACS CHEM BIOL,ACS CHEM BIOL,2010,2010,5,577-587硒对植物体内汞的生物学行为及效应硒对植物体内汞的生物学行为及效应的影响的影响植物是食物链的基础，降低植物重金属的含量或改变其形态是防止重金属

10、危害人体健康的有效途径。 利用上海同步辐射装置研究植物对汞的吸收以及汞在植物体内的分布、转化、蓄积情况，研究硒对这些过程的影响，包括这种影响的剂量效应关系以及影响机制。实验方案（研究内容、实验方法）实验方案（研究内容、实验方法） 植物培植物培养养汞处理硒&汞处理对照植物对照植物实验植物实验植物根根茎茎叶叶根根茎茎叶叶冷冻切片40m薄片薄片叶基部叶基部叶尖部叶尖部干燥保存干燥保存X射线荧光成像射线荧光成像XAS生长状态生长状态元素含量元素含量-0.10.00.10.20.30.40.50.60.70.80.90 0.01 0.1 1 10 100 mercury0 0.01 0.1 1

11、10 100 selenite selenate-0.10.00.10.20.30.40.50.60.70.80.90 0.01 0.1 1 10 100 Hg 0.01 ppm Hg 1 ppm Hg 100 ppmseleniu treatment (ppm)-0.10.00.10.20.30.40.50.60.70.80.90 0.01 0.1 1 10 100 Hg 0.01 ppm Hg 1 ppm Hg 100 ppmmercury treatment (ppm)selenite treatment (ppm)selenate treatment (ppm)inhibition i

12、ndexabc不同剂量硒汞条件下的植物生长抑制指数05101520253035e0 0.01 0.1 1 10 100 0 0.01 0.1 1 10 100 0 0.01 0.1 1 10 100 012345f012345d030060090012001500g0 0.01 0.1 1 10 100 0 0.01 0.1 1 10 100 0 0.01 0.1 1 10 100 0408012016020001020304050ih0123456rootstem0 0.01 0.1 1 10 100 0 0.01 0.1 1 10 100 cb Na2SeO3 Na2SeO4 Na2SeO

13、3 Na2SeO4 Na2SeO3 Na2SeO40 0.01 0.1 1 10 100 0.00.51.01.52.0leaf012345amercury content (g kg-1 dry weight)Se concentration in medium (ppm)*低汞条件下，硒可增加植物体内的汞积累高汞条件下，硒降低植物汞积累（茎除外）不同浓度硒汞条件下培养植物组织中的汞蓄积a,b,c Hg浓度0.01 ppmd,e,f Hg浓度1 ppmg,h,i Hg浓度100 ppmHgHgHgHgSeSe1225012300123501240012450CH3HgCysCH3HgGSHHg(GSH)3HgOleaf Hg&Se treatmentstem Hg&Se treatmentroot Hg&Se treatmentleaf Hg treatmentstem Hg treatmentHg MetHgCl2root Hg treatment12200123001