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1、(SEM光谱仪(FT-IR)和X 射线光电子能谱仪(XPS)对SGHs氮离子注入的三维石墨烯(N+/3D-SGHs 、 乙二胺还原的三维石墨烯的水接触角。结果表明:3D-SGHs 3D/ED-SGHs3D-SGHs而化学改性(乙二胺100m,氮含量与N+/3D-SGHs(11020个/cm2)细(ROS172 细胞在 3D-SGHs 表面黏附、生长的情况明显优于二维石墨烯表面,说明 3D-SGHs 为细胞提供了广阔的生长空间,突破了二维材料的空间限制;细胞N+/3D-SGHs 3D/ED-SGHs 3D-SGHs 强,细胞的生长周期N+/3D-SGHs 来说,随着N+注入剂量的增加,N+/3D
2、-SGHs 显示出更加优越的细胞相容性; 3D/ED-SGHs 与同等氮含量的 N+/3D-SGHs(注入剂量为 11020个/cm2)相比,有着更优的细胞相容性。I:三维石墨烯,氮离子注入的三维石墨烯,乙二胺还原的三维石墨烯al construction and poor hydrophilicity are always two barriers for graphene application in biomaterials field. Herein, 3D self-graphene (3D-SGHs) was produced via one-step hydrothermal s
3、ynthesis to break the confine of the degree-freedom.al construction and poor hydrophilicity are always two barriers for graphene application in biomaterials field. Herein, 3D self-graphene (3D-SGHs) was produced via one-step hydrothermal synthesis to break the confine of the degree-freedom. The mech
4、anical strength, holes spatial structure of 3D-SGHs were adjusted by thereaction time andthe concentration of graphite oxide. And those wrinkles and pores in 3D-SGHs can provide a largesurface area and 3D stereo ecological simulation environment for cells growth. implanted three-al self-assembly gra
5、phene (N+/3D-which achieved by ion ion on 3D-SGHs and the ethylenediamine graphene (3D/ED-SGHs) which achieved by adding o synthetic s of hydrothermal reaction roduced to solve the problem poor morphology and component characters of 3D-SGHs, N+/3D-SGHs ties and 3D/ED-SGHs were studied by the spectro
6、scopy (Raman), Scanning electron microscopy (SEM), Fourier infrared spectroscopy (FT-IR) and X-ray photo electron spectroscopy (XPS). water contact angles of les were recorded using the optical contact-inclinometer. 3D-SGHs exhibits a monolith of continuous and porous structure with numerous irregul
7、arly pores, 2-5 meter. It t the holes 3D-SGHs are formed by bending stack of graphene layers in hydrothermal Compare with 3D-SGHs, N+/3D-SGHs have no distinct but the hydrophilicity of N+/3D-SGHs is enhanced based contact angle ion does measurements. SEM observation t nitrogen ion destroy the 3D-SGH
8、s surface structure. However, rture of 3D/ED-SGHs 100 m, which is n 3D-SGHs. The 3D/ED-SGHs has good which nitrogencontent is same with the N+/3D-SGHs (withthe 11020ty In order to reflect the correlations n patibility and 3featuresofthe les, the cell viability invitromeasuredby MTTcolorimetricusing
9、the mouse-fibroblast cells (L929) and osteoblast (ROS172) were used ively study the cells on 3D-SGHs, N+/3D-SGHsandfeaturesofthe les, the cell viability invitromeasuredby MTTcolorimetricusing the mouse-fibroblast cells (L929) and osteoblast (ROS172) were used ively study the cells on 3D-SGHs, N+/3D-
10、SGHsand 3D/ED-al rved as the control group. The amounts of adhering on 3D-SGHs are n the control group. 3D-SGHs have larger surface areas, which provide more for cell proliferation. The living numbersonN+/3D-SGHsand 3D/ED-SGHsarealways nthoseon 3D-during cell culture. Besides, as the increase doped dose, N+/3D-SGHs better patibility.These results t the nitrogen can really the cell proliferation and viability. Compared with N+/3D-SGHs (with 1 rum albumin (BSA) was used to test the ty on of 3D-SGHs, N+/3D-SG
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