Kuguaglycoside-C-DataSheet-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEKuguaglycosideCCat.No.:HY-113915CASNo.:1041631-93-9分⼦式:C₃₆H₅₆O₈分⼦量:616.83作⽤靶点:Apoptosis作⽤通路:Apoptosis储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性KuguaglycosideC⼀种三萜糖苷，可以从Momordicacharantia的叶⼦中分离出来。KuguaglycosideC诱导神经母细胞瘤细胞的caspase⾮依赖性DNA切割和细胞死亡。KuguaglycosideC还显增加凋亡诱导因⼦(AIF)的表达和裂解。体外研究KuguaglycosideC(0-100μM,48h)inducessignificantcytotoxicityagainsttheIMR-32cells,withanIC50of12.6μM[1].KuguaglycosideCinducesnuclearshrinkageatahighconcentration(100μM)inexaminationbyHoechst33342staining,butnoapoptoticbodieswereobservedonflowcytometry[1].KuguaglycosideC(30μM,48h)showsnoactivationofcaspase-3orcaspase-9,butshowsincreaseinthelevelsofTRADD,AIFandDFF45,anddecreaseinthelevelsofRIP1andsurvivin[1].CellViabilityAssay[1]CellLine:IMR-32cellsConcentration:0,0.3,1,3,10,30,100μMIncubationTime:48hResult:InducedsignificantcytotoxicityagainsttheIMR-32cells,withanIC50of12.6μM.WesternBlotAnalysis[1]CellLine:IMR-32cells1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEConcentration:30μMIncubationTime:0,2,4,8,24,48hResult:ActivatedbothtypeI(caspase-dependent)andtypeII(caspase-independent)DNases.ShowedsignificantPARPcleavage.ShowedincreaseinthelevelsofTRADD,AIFand

DFF45,anddecreaseinthelevelsofRIP1andsurvivin.Proteinlevelsofcleavedcaspase‐3,‐7and‐9werenotincreased.Theexpressionlevelsofp‐p38MAPK,p‐JNK,Bax,Bcl‐2andcytochromecremainedunchanged.CellCycleAnalysis[1]CellLine:IMR-32cellsConcentration:1,3,10,30,100μMIncubationTime:48hResult:Hadnoeffectonthecellsofanyphaseofthecellcycleintheneuroblastomacellline.REFERENCES[1].TabataK,etal.KuguaglycosideC,aconstituentofMomordicacharantia,inducescaspase-independentcelldeathofneuroblastomacells.CancerSci.2012Dec;103(12):2153-8.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.T