Hoechst-33342-trihydrochloride-solution-生命科学试剂-MCE

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEHoechst33342trihydrochloride(solution)Cat.No.:HY-DY1005CASNo.:875756-97-1分⼦式:C₂₇H₃₁Cl₃N₆O分⼦量:561.94作⽤靶点:Autophagy作⽤通路:Autophagy储存⽅式:-20°C,protectfromlight.Avoidrepeatedfreezingandthawing*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light.Avoidrepeatedfreezingandthawing)BIOLOGICALACTIVITY⽣物活性Hoechst33342trihydrochloride(solution)Hoechst系列的标记染料。Hoechst系列活细胞核标记染料。Hoechst通过结合DNA双链中的⼩沟⽽结合核酸。Hoechst倾向于结合富含A/T的DNA链。同时，富含A/T的双链DNA使荧光强度显著增强。Hoechst可穿过细胞膜，结合活细胞或定细胞。Hoechst染料的荧光强度随溶液pH升⾼⽽增强。溶剂及浓度：⽆⽔：10mg/mL体外研究GeneralProtocolPreparationofHoechstworkingsolutionThecorrespondingstocksolutioncanbedilutedaccordingtotheactualsituation.NotethatifthesolventisDMSO,thecytotoxicityofDMSOmustbeconsidered,andasolventcontrolshouldbeprepared;ifthesolventispurewater,theworkingsolutionneedstobefilteredandsterilizedbeforeaddingcells.1.Cellstaining2.1Suspensioncells(6-wellplate)a.Centrifugeat1000gat4°Cfor3-5minutesandthendiscardthesupernatant.WashtwicewithPBS,5minuteseachtime.Thecelldensityis1×106/mL.b.Add1mLofworkingsolution,andthenincubateatroomtemperaturefor3-10minutes.c.Centrifugeat400gat4°Cfor3-4minutesandthendiscardthesupernatant.d.WashtwicewithPBS,5minuteseachtime.e.Resuspendcellswithserum-freecellculturemediumorPBS.Observationbyfluorescencemicroscopyorflowcytometry.2.2Adherentcellsa.Cultureadherentcellsonsterilecoverslips.1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEb.Removethecoverslipfromthemediumandaspirateexcessmedium.c.Add100μLofworkingsolution,gentlyshakeittocompletelycoverthecells,andthenincubateatroomtemperaturefor3-10minutes.d.Washtwicewithmedium,5minuteseachtime.Observationbyfluorescencemicroscopyorflowcytometry.StorageStoreinseparatecontainerstoavoidrepeatedfreeze-thawcycles.-20°C,1year.ProtectfromlightPrecautions1.PleaseadjusttheconcentrationofHoechstworkingsolutionaccordingtotheactualsituation.2.ThisproductisforR&Duseonly,notfordrug,household,orotheruses.3.Foryoursafetyandhealth,pleasewearalabcoatanddisposableglovestooperate.REFERENCES[1].ChazotteB.LabelingnuclearDNAwithhoechst33342.ColdSpringHarbProtoc.2011Jan1;2011(1):t5557.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.For