模拟失重大鼠下丘脑不同部位的Fos和GFAP表达.doc

FosGFAP , :;Fos; :.FosGFAPABC4AS.;AS. Keywords:hypothalamus;geneFos;glialfibrillaryacidicprotein;weightlessnesssimulation Abstract:AIMToresearchthefunctionsofrathypothala-musunderlong-termsimulatedweightlessnesscondition.METHODSByusinganti-Fosandanti-GFAPimmunohis-tochemicalABCmethodsweobservedchangesofhypothala-musneuronsandastrocytesin4wksimulatedrats.RESULTSExpressionsofFos-andGFAP-positivecellswereonlyfoundinparavetricularnucleiofthehypothalamuses-peciallyinmangocellularpartsupraopticnucleiandsuprachiasmaticnucleiwithnegativereactioninotherareas.Thereactiveastrocyteswerefeaturedwithhypertrophyof thecellbodyandcytoplasmicprocesses.CONCLUSIONThesenucleiinhypothalamusmayparticipateinthereactiontolong-termweightlessnessandprobablybeassociatedwithincreasedsecretionofvasopressure. 0 -.-1.PVNAVPAVP23. AS45.14dGFAP6GFAP. 4wkFosGFAPPVNSONAS. 1 1.1 1.1.1160gSpraque-Dawley.6.7-30.223212h. 1.1.240mgkg-1ip100mL40gL-10.1molL-1pH7.4500mL1.5h200gL-14.30m10mmolL-1PBS. 1.1.3ABC3gL-1TritonX-100PBS30min.:FosABC:Fos:Fos11000SantaCruz48h4;IgG1500Sigma2h;-HRPABC1500Sigma2h-DAB-.PBS310min.:GFAP:GFAP13000Sigma48h4;IgG15002h;-HRPABC15002h-DAB-. . 1.2 1.2.1n=2FosGFAPFosGFAP. 1.2.210mmolL-1PBSFosGFAPABC. 2 2.1 2.1.1FosPVNSONSChFig1-AB. 2.1.2GFAPGFAPFig2-AB. 2.2 2.2.1FosPVNFig1-AFig1-A1SONFig1-BSCh. 2.2.2GFAP:GFAPAS;FosGFAPFig2-AB. 3 3.1NASA7120d. 3.2FosGFAP 3.2.1Fos.4wkFosPVNSONSCh. PVNPBNMVZPVNFosSON23. 3.2.2FosGFAPGFAP89.ASAS.4wkGFAPFosAS.GFAPFosAS.GFAP. 12 3.3FosA1A210NTSVLMPVN11.LiYWPVNSONFosPVN12.A2NTSA22.1314;NTSVLMPVNAVP;PVNFos15PVN. : 1BlomqvistCGBuckeyJCGaffneyFALaneLDLevineBDWatenpaughDE.Mechanismsofpostflightorthostaticintoler-anceJ.JGravitPhysiol1994;11:122-124. 2FagetteSSomodyLBouzeghraneFCottetEmardJMGharibCGauquelinG.Centralandperipheralsympatheticac-tivitiesinratsduringrecoveryfromsimulatedweightlessnessJ.JApplPhysiol1995;796:1991-1997. 3MailletATitzeJGushinVNichiporukIKirschKAGharibCGauquelinKochG.Hormonalchangesduringa20wkconfinementJ.AviatSpaceEnvironMed1998;6911:1045-1051. 4GroscheJMatyashVMollerTVerkhratskyAReichenbachAKettenmannH.Microdomainsforneuron-gliainteraction:ParallelfibersignalingtobergmannglialcellsJ.NatNeu-rosci1999;22:139-143. 5AlvarezMaubecinVGarcia-HernandezFWilliamsJTVanBockstaeleE.FunctionalcoupingbetweenneuronsandgliaJ.JNeurosci2000;2011:4091-4110. 6DayJRFrankATOCallaghanJPDeHartBW.EffectsofmicrogravityandbonemorphogeneticproteinonGFAPinratbrainJ.JApplPhysiol1998;852:716-722. 7ChenJMaJDingZPZhangLF.Amodifiedtail-suspensionmodelforsimulatinglong-termweightlessnessJ.ZhonghuaHangkongYixueZazhiChinSpaceMedSciJ1993;132:148-152. 8RutkaJTMurakamiMDirksPBHubbardSLBeekerLEFukuyamaKJungSTsuguSAMatsuzawaK.RoleofglialfilamentsincellandtumersofglialoriginJ.JNeurosurg1997;87:420-430. 9GomesFCPaulinDMouraNetoV.Glialfibrillaryacidicpro-teinGFAP:ModulationbygrowthfactorsanditsimplicationinastrocytedifferentiationJ.BrazJMedBiolRes1999;32:619-631. 10ChalmersJPilowskyP.Brainstemandbulbospinalneuro-transmittersystemsinthecontrolofbloodpressureJ.JHy-pertens1991;98:675-694. 11KannanHYamashitaH.Connectionsofneuronsintheregionofthenucleustractssolitariuswiththehypothalamicparaven-tricularnucleus:TheirpossibleinvolvementinneuralcontrolofthecardiovascularsystemintheratsJ.BrainRes1985;3291/2:205-210. 12LiYWDampneyRAL.ExpressionofFos-likeproteininbrainfollowingsustainedhypertensionandhypotensioninconsciousrabbitsJ.Neurosci1994;613:613-634. 13SuCJBaoJXRaoZRZhangLF.Fosexpressionintheneu-ronsofmedullaoslongataandchangesofneuronsizeinthein-termediolateralnucleusofthespinalcordduringsimulatedweightlessnessJ.ZhongguoShenjingJiepouxueZazhiChinNeuroanatomyJ2000;161:52-56. 14SomodyLFagetteSFrutosoJGharibCGauquelinG.Recordingheartrateandpressureinratsduringparabolic