【doc】阳离子脂质体-非编码质粒dna复合物抗小鼠结肠癌的实验研究

阳离子脂质体非编码质粒DNA复合物抗小鼠结肠癌的实验研究21ACTINNORTH2ERNJINBDAACTINNORTHERNJIEDAACTINFIG21NORTHERNDOTBLOTANALYSISOFAACTINFFFANFTAFDO11BNOSNORTHERNJINBDBNOSMRNAJIEDBNOSMRNAJIWFC2ZFIG11NORTHERNDOTBLOTANALYSISOFBNOS2FDONNOSRNFTNOSRNF11312CCDNA4N,C37,A5RBNOSHIND,/HIND,QNAACTINPSTI/PSTINR,41JTAELRXC4BNOS110KBAACTIN018KBCDNA,TEL20,ANC11313CNORTHERNI/DD,CIKC110Y,CN61RNA,KFIYCMRNA,OD260/OD280DRNA,4M8OD260/OD280J210OBNORTHERNJICM8MDM20AGRNA/MOZSNA32PDCTPN2,SEPHDEXG50,4WC8,YEDNZ60,WCZ5108CPM/AGBJC2JTIJWXU,T,70,AWCW14,BWC2C2TWC11314C/IUJIOC2FZ/MRNAOZPAACTINJZC2C/DDBNOSJI,UDMRNAJIWZC2F1C/DOGAACTINMRNA,TYMRNAF2C3CDOCNOVC,FNNOVFNNOSGV2,VDNOS5FZRDNO5FCNOS,PF2,OF3GNOCDBNOS3,EONOSNOSCNO6,ROYESZNOSNMDA,YQZO4CDAWZDBNOSMRNABDWDCVNOSNONGD,PYNOVDC2,FTEOYCF4VGABAVFOJYC,NOZVYDY4AOB,I3IHC6PBNOSMRNAD,NONGD,E3CDBUISSON4NOSBN5,62NOOD4BCPEN4BNOS,4VBNOSMRNA6NOS,YCU6I4VBNOS5OYC4CA1CHOMCZYNSLEIP,SACCHINSINGLESTEPMETHODOFRNAISOLATIONBYACIDGUANIDINIUMTHIOCYANATEPHENOLCHLOROFORMEXTRACTIONJANALBIOCHEM,1987,1621562HASHIKAWAT,LEGIOMG,HATTORIR,ETALNI2TRICOXIDESYNTHASEIMMUNOREACTIVITYCOLOCAL2IZEDWITHNADPHDIAPHORASEHISTOCHEMISTRYINMONKEYCEREBRALCORTEXJBRAINRES,1994,641343KNOWLESRG,PALACIOSM,PALMERRM,ETALKINETICCHARACTERISTICSOFNITRICOXIDESYNTHASEFROMRATBRAINJBIOCHEMJ,1990,2692074ROYESLF,FIGHERAMR,FURIANAF,ETALIN2VOLVEMENTOFNOINTHECONVULSIVEBEHAVIORANDOXIDATIVEDAMAGEINDUCEDBYTHEINTRAS2TRIATALINJECTIONOFMETHYLMALONATEJNL,5,3665B,LN,VLNX2N,3,4L3F10020179200704081304GF2TREATMENTOFMURINECOLONCANCERWITHCOMPLEXESOFDNANFDQYCATIONICLIPOSOMEANDNONCODINGPLASMIDDNA,03,WANGZHONGYU,TANGMINGHAI,CHENPING,ETAL3,GC610041STATEKEYLABORATORYOFBIOTHERAPY,WESTCHINAHOSPITAL,SICHUANUNIVERSITY,CHENGDU610041,CHINACCGF2DNANFDQZ4CFDQH/,OGF2DNANQ,Y/F,/,C0FIUCGF2DNAN,CDR973DANO2004CB518807WCT1982,Y3CC30DENGHONGXYAHOOCOMCN3182007,224CCN511356/R8ROMDIERENTGROUPSNACTINORTHERNDOTBLOTROMTHECONTROLEHEACTINDOTBLOTOTHEEPILEPTICSMALLERHIPPOCAMPUSISTHESAMEASTHECONTROLSROMTHEEPILEPTICANDTHECONTROLSBMADOTBLOTROMTHECONTROLHIPPOCAMPUSEHEBMAOEPILEPTICRATHIPPOCAMPUSISANDLIGHTEREUROSCIETT2007211UISSONAAKHMECHEERRECHIACITRICOIDEANENDOGENOUSANTICONVULSANTSUBSTANCEJEUROREPORT199444420070110,/H/,W,FDWFBWSCGF2DNAGNOFDQCGF2DNAQIR735135CCCN2ACCABSTRACTOBJECTIVETOINVESTIGATETHEANTITUMOREFFECTOFCOMPLEXESOFCATIONICLIPIDANDNONCODINGPLASMIDDNAINMURINECOLONCANCERMETHODSMURINELUNGMETASTASISMODELWASESTABLISHEDBYINTRAVENOUSINJECTIONOFCT26MURINECOLONCAR2CINOMACELLLINECELLSINTOBALB/CMICE,WHILESUBCUTANEOUSTUMORMODELWASESTABLISHEDBYSUBCUTANEOUSLYINJECTIONTHETU2MORBEARINGMICEWEREDIVIDEDINTOTHREEGROUPSANDINJECTEDINTRAVENOUSLYWITHONEOFTHEFOLLOWINGPREPARATIONSCATIONICLIPID/NOCODINGPLASMIDDNACOMPLEX,CATIONICLIPIDANDNSRESPECTIVELYTHETUMORVOLUME,THENUMBEROFMETASTASISFOCI,ANDTHEHISTOLOGICALCHANGESOFTHETUMORINTUMORBEARINGMICEWERETESTEDAFTERTHERAPYRESULTSTHETUMORVOLUMEANDTHENUMBEROFMETASTASISFOCIINMICETREATEDWITHCATIONICLIPID/NOCODINGPLASMIDDNACOMPLEXWERELESSTHANOTHERGROUPSCONCLUSIONTHERESULTSSUGGESTTHATTHECOMPLEXOFCATIONICLIPIDANDNONCODINGPLASMIDDNACOULDINHIBITMURINECOLONCANCERKEYWORDSCATIONICLIPOSOMENONCODINGPLASMIDDNACOLONCANCERGENETHERARECEIVEDDATE20070526CCG6IOX,DNAGN,IDNAWC,EIFCCZGOH01L4N,IDNAVDNABVFC28C23,EYCSYT,OGC,SYGF2DNAGNCATIONICLIPOSOMEPLASMIDDNACOMPLEXES,CLDC3/,OY/FDFVCCLDCFFD3,SWC2F,E3/CDY,CLDCSOCW4FDQH/3,CLDC/6SFV3,CLDC/ZA,SUD/,UN/Z4YC1C111CF2DNAPORFMCS3B,KSY,KINVIVOGENFDQ0CT26ATCCAMERICANTYPECULTURECOLLECTIONRPMI1640GIBCOBALB/CFD68,C112C11211CCT26RPMI164010VT,37,A,5CO2C11212CDNAC2G4NGDNAN4N1121211CDNA4N4RU,U,JAKTAEXPLORERF,VDNAINNC1121212CG4NIZ3746IYZDOTDO,IINGJ,32HI6,VI5C5F,Y52H,SI/3FJFE4NG,I4,ANC1121213CGDNAN4N3I5CIDNAGO1745QN,H20I4GGDNANC11213C,4C33FDQCT26,VTC23DRPMI1640T3,I,AZ215106CELLS/MLC1,FDWOC51050CT262H/,FDH31050CT26C11214C1121411C,FD/,I45FDZIZ3GF2DNANC2G3I5C/83,FDWO60AGDNA300AGG/Q,4,5,G3I5IK300AGG200AL3I5CC/826CFD,CFDC2C2C2WCFD/,V10X,NQHE,/2U,F2FC1121412CH/,FD/,I18FDZIZ3GF2DNANC2G3I5C/88/4050MM3FDWO60AGDNA300AGG/,4,5,G3I5IK300AGG200AL3I5CC3BNZY/C2,/OHF1MM301522C/41T/C100,T/C/C11215CII,15ZOFCC1DNN/34/826CFD,CFDJ,GF2DNANCLDCZ83,CIZ10386,3I5Z10483P0105,1C22CCLDC01200105W014701293I501540135P0105,2CACLDC,BCI,C3I5CI11212CCLDCH/34,3Y/,/836CFD,YCFD/,CLDC/WF3I5CIP0105,/4IKZ67175811,3C213CCFDWFFICFDWFCLDCFDWFWF/4,HEWZCLDCFDWFBWSG3I5CI5,EFDFC2FC2FW2F0WZC3C8Y,DSOGF2DNANCLDCW4FDQH/3C/6PGF2DNAVGN,EGFF2DNAS3W/2CI6ZGOWZ,EF2DNLZBV,EOWZ,EGYGF2DNN,NPSW418WESTCHINAMEDICALJOURNAL2007,VO1122,NO14CCN511356/R8111APPEP00221AAA51XVDNAG,E3/3C2131CLDC/Z4,YTS,/FDFVCCLDCNFZ3,SW,F,E3/CZCLDCSMZSGIF,FCLDCDNA/6DNAXFCPG,YCPGSBRTLR9NE6TH1,3,TIL6,IL12,IFNA4,EDNACPGXFOWH5C26,YIFNATNFA5WH7CXFCPG3PVCOYZNK6CLDC3/I,CLDCFDYCFNK,ENKF,IFNAWE3/ENK,CLDCN/I2COYZCD8T6,ZCD8T,CLDCN/YP8C29COYCLDCV3/04,63O,4G,T3CD8TNKZ,TI/ET3/CTLSZ,GGT/2CACLDC,BCI,C3I5CI41CLDC/T,DKAMSTOCK10I0125MG/MLCLDC,ZCLDC/V3G4I6PCLDCV,ZCLDC8APCWV3G4,4,V3GWO10CF,CLDCN/V36/Z4YC8Y,DCLDCCIFDWFBWS,6PFDVVFDSWFB3V,ZOYOGDNAN24FVVFD809011CF,CLDCF3,OCLDCFD24FALTAST56,DNA,0CI5CLDC3O1036CY,CLDC/ZA,SUD/,UN/Z4MDYC4CA1REILLYMS,BOEHMT,SHINGY,ETA1ENDO2STATINANENDOGENOUSINHIBITOROFANGIOGEN2ESISANDTUMORGROW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