“细胞生物学”教学大纲

1、细胞生物学”教学大纲四川大学生命科学学院AGeneralOutlineoftheCellBiologyCollegeofLifeSciences,SichuanUniversity一、课程基本信息课程名称（中、英文）：细胞生物学CellBiology课程类别：专业基础课主要教材：细胞生物学第4版，翟中和、王喜忠、丁明孝，高等教育出版社，2011.6参考书：CellandMolecularBiology:ConceptsandExperiments,6thEdition,Karp,2010MolecularBiologyoftheCell,5thEdition,Albertsetal.,2008

2、学时：48学分：3课程地位：国家精品课程主讲教师：邹方东、王喜忠、李娟、李沁桐、张咏梅、杨军等二、教学目的及要求细胞生物学作为现代生命科学发展的分支学科，已被教育部列为理科生物学教学的重点基础课程。细胞作为有机体结构与生命活动的基本单位，既是生命科学发展的生长点，又是生命科学发展的汇聚点，因此，细胞生物学教学在生命科学综合素质教育及实验技能综合培养等方面都具有重要作用。细胞生物学教学目标是使学生既掌握本学科的发展简史和前沿领域，又掌握细胞生物学的基础知识、基本概念和基本理论，使学生受到基本科学思维训练，同时使学生学会学习，具有自我开拓可获得知识和利用信息的能力。TeachingObjectiv

3、es:Cellbiologyisoneofthemostimportantcoursesofcurrentbiology,andhasbeenlistedasakeycurriculumofsciencesbytheMinistryofEducationofChina，andexcellentcurriculumofSichuanUniversityandSichuanprovince.Asthebasicunitofstructureandfunctionforallorganisms,cellisboththestartandconvergepointsoflifescience.Thet

4、eachingtargetofcellbiologyistoacquaintthestudentswiththebasicknowledge,conceptsandfundamentaltheories,aswellasthebriefhistoryandmostadvanceddomainofthesubjectbeingtaught.Furthermore,thestudentsaretocomeundertrainingsontheirthinkingtomastertheskillsoflearning,soastobecapableofexploitingtheirtalentson

5、gettingknowledgeavailableandutilizinginformation.教学基本特色：1. 本课程目前是国家优秀名牌课程和国家精品课程，采用双语和多媒体教学，课堂教学效果显著，课程在国内具有很好影响力和辐射作用；2. 采用“研究型教学”理念，教授内容新，既体现细胞生物学基本知识，又反应生命科学发展前沿和热点领域；3. 按着学科内容体系、学科内在联系和主从关系，依次确定知识块一知识单元f知识点。知识块、知识单元和知识点的确定具有较强的可操作性，是在遵循教学规律和科学发展规律的基础上，能有效地处理好传统内容和现代内容的关系，处理好传授知识和能力培养的关系。4. 所界定的教

6、学基本留有余地，以利创新，既有利于面向21世纪课程体系及教学内容的改革，又有利于教师在教学中主导作用和学生主体作用的发挥。Basicteachingcharacteristics1. Cellbiologyisoneofthecountry'sfirst-classnamebrandcourses,whichisperformedbilinguallyandemployingthemulti-media.Theteachingeffectinclassisratherremarkableandtheconstructionofthecourseisonthetopinternal.2.

7、 Frequentupdating.Notonlyanembodimentofbasicknowledge,butalsoareflectionofprecedingfieldsandhotspotsoflifesciences.3. Takingthepoliceof“educationshouldfacethemodernization,facetheworldandfacethefuture”astheguideline.Accordingtothecontentsystem,internalconnectionsaswellastheprincipalandsubordinaterel

8、ationshipsofthesubject,weestablishedtheroute:learningblockfunitfpoint.Andthishasbeenprovedexercisable,whichfollowstheteachingrulesandscientificdevelopmentallawsanddisposeswelloftherelationsbetweentraditionalandmoderncontents,andtherelationbetweenteachingknowledgeandtrainingabilityaswell.4. Leavesome

9、roomforinnovation,whichinfavorofreformsontextbookseriesfor21stcenturyandteachingsubstances.三、教学内容知识块一细胞生物学概论及其研究方法LearningBlock1ReviewofCellBiologyandItsResearchingMethods知识单元一第一章绪论（2学时）第一节细胞生物学研究的内容与现状知识点一、了解细胞生物学是现代生命科学的重要基础学科知识点二、了解细胞生物学的主要研究内容第二节细胞学与细胞生物学发展简史知识点一、了解细胞的发现知识点二、了解细胞学说的建立及其意义知识点三、了解

10、细胞学的经典时期知识点四、了解实验细胞学与细胞学的分支及其发展知识点五、了解细胞生物学学科的形成与发展知识点六、了解细胞生物学的主要学术组织、学术刊物与教科书Unit1Chapter1IntroductionSection1ContentsandActualityofResearchesonCellBiologyPoint1.KnowthatcellbiologyisafundamentalsubjectinmodernlifesciencePoint2KnowtheimportantresearchingcontentsoncellbiologySection2ABriefHistoryof

11、CytologyandCellBiologyPoint1KnowthediscoveryofcellPoint2KnowtheestablishmentofcytologyanditssignificancePoint3KnowtheclassicalperiodofcytologyPoint4KnowtheembranchmentanddevelopmentofexperimentalcytologyandcytologyPoint5KnowthesubjectsestablishmentanddevelopmentofcellbiologyPoint6Knowthemainacademic

12、organizations,issuesandtextbooks知识单元二第二章细胞的统一性与多样性（2学时）第一节细胞的基本特征知识点一、掌握并理解细胞是生命活动的基本单位知识点二、掌握细胞的基本共性第二节原核细胞与古核细胞知识点一、了解最小最简单的细胞支原体知识点二、了解原核细胞的两个代表细菌和蓝藻知识点三、了解古核细胞（古细菌）第三节真核细胞知识点一、理解并掌握真核细胞的基本结构体系知识点二、理解细胞的大小及其影响因素知识点三、理解并掌握原核细胞与真核细胞的比较知识点四、理解并掌握植物细胞与动物细胞的比较第四节非细胞形态的生命体病毒知识点一、了解病毒的基本知识知识点二、了解病毒在细胞内的

13、增殖知识点三、理解病毒与细胞在起源与进化中的关系Unit2Chapter2DiversityandCommonalityofCellsSection1BasicConceptsofCellsPoint1MasterandapprehendthatcellsarethebasicunitsoflifePoint2MasterthecommonnessofcellSection2ProkaryoticandEukaryoticCellPoint1KnowtheminimalandsimplestcellmycoplastPoint2Knowtworepresentationofprokaryoti

14、ccellbacteriaandcyanobacteriaPoint3KnowarchaeobacteriaSection3EukaryoticCellPoint1ApprehendandmasterthebasicstructuralsystemofeukaryoticcellPoint2ApprehendthesizeofcellsanditsregulatorsPoint3ApprehendandmasterthecomparisonofprokaryoticandeukaryoticcellsPoint4Apprehendandmasterthecomparisonbetweenpla

15、ntcellandanimalcellSection4NoncellularlivingOrganismVirusPoint1KnowthebasicknowledgeofvirusPoint2Knowthatvirusproliferate（replicate）incellsPoint3Apprehendtherelationbetweenvirusandcellintheoriginandevolutionoflife知识单元三第三章细胞生物学研究方法（3学时）第一节细胞形态结构的观察方法知识点一、掌握并理解光学显微镜技术知识点二、掌握并理解电子显微镜技术知识点三、了解扫描隧道显微镜第二节

16、细胞及其组分的分析方法知识点一、理解用超速离心技术分离细胞组分知识点二、了解细胞成分的化学显示方法知识点三、理解并掌握特异蛋白抗原的定位与定性知识点四、了解细胞内特异核酸序列的定位与定性知识点五、了解定量细胞化学分析技术,理解并掌握细胞分选技术第三节细胞培养与细胞工程知识点一、掌握细胞培养类型和方法知识点二、掌握细胞工程的主要成就第四节细胞及生物大分子的动态变化知识点一、理解并掌握荧光漂白恢复技术知识点二、了解单分子技术与细胞生命活动的研究知识点三、理解酵母双杂交技术知识点四、理解荧光共振能量转移技术知识点五、理解放射自显影技术第五节模式生物与功能基因组的研究知识点一、了解细胞生物学研究中常用

17、的模式生物知识点二、了解突变体制备技术知识点三、了解蛋白质组学技术Unit3Chapter3MethodologyofCellBiologySection1MethodsonCellStructureObservationPoint1ApprehendandmasteroflightmicroscopyPoint2ApprehendandmasterofelectronmicroscopePoint3KnowscanningtunnelingmicroscopeSection2AnalyticalMethodsofCellComponentsPoint1Apprehendusingsuper-

18、centrifugationtoseparatecellularcomponentsPoint2Knowthemarkingmethodsofcomponentsincell.Point3ApprehendandmasterlocalizationandqualitativeanalysesusingspecialproteinantigenPoint4KnowthelocalizationandqualitativeanalysesofspecialintracellularnucleicacidsPoint5Knowthequantitativeanalysesofcytochemistr

19、y,apprehendandmasterflowcytometrySection3CellCultureandCellEngineeringPoint1MastertypesandmethodsofcellincubationPoint2MastermainachievementsofcellengineeringSection4DynamicsofCellandBiomacromoleculesPoint1ApprehendandmasterfluorescencephotobleachingrecoveryPoint2knowtheSingleMoleculeDetectionPoint3

20、Apprehendyeasttwo-hybridsystemPoint4ApprehendfluorescenceresonanceenergytransferPoint5ApprehendthetechniquetotraceintracellulardynamicsynthesisofmacromoleculesusingradioautographySection5ModelOrganismsandFunctionalGenomeResearchPoint1KnowmodelorganismsusedincellbiologyPoint2KnowhowtopreparemutantsPo

21、int3Knowproteomemethods知识块二生物膜系统LearningBlock2BiomembraneSystem知识单元一第四章细胞质膜（2学时）第一节细胞质膜的结构模型与基本成分知识点一、掌握并理解细胞膜的结构模型知识点二、掌握膜脂的种类及运动方式知识点三、掌握膜蛋白种类及常用去垢剂第二节生物膜基本特征与功能知识点一、理解并掌握膜的流动性知识点二、理解并掌握膜的不对称性知识点三、了解膜骨架与细胞表面的特化结构知识点四、了解细胞膜的功能Unit1Chapter4CellMembraneSection1StructureModelofCellMembraneandconstitue

22、ntsPoint1MasterandapprehendmembranestructurePoint2MastertypesofmembranelipidsandmotilitiesPoint3MastertypesofmembraneproteinsanddetergentsSection2BasicPropertiesandFunctionsofBiomembranePoint1ApprehendandmasterfluidityofmembranePoint2ApprehendandmastertheasymmetryofmembranePoint3Knowmembraneassociat

23、edcytoskeletonandspecializedstructuresoncellsurfacePoint4Knowfunctionsofmembrane知识单元二第五章物质的跨膜运输（2学时）第一节膜转运蛋白与小分子物质的跨膜运输知识点一、掌握载体蛋白和通道蛋白转运特点知识点二、掌握小分子物质跨膜运输类型及特点第二节ATP驱动泵与主动运输知识点一、理解并掌握Na+-K+泵、Ca2+泵工作原理与功能知识点二、掌握V型质子泵和F型质子泵的功能知识点三、掌握ABC超家族的功能知识点四、了解离子跨膜运输与膜电位第三节胞吞作用与胞吐作用知识点一、理解并掌握吞噬作用过程与功能知识点二、理解并掌握网

24、格蛋白依赖的胞饮作用过程与特点知识点三、了解其他类型的胞饮作用知识点四、理解并掌握胞吞作用对信号转导的下调或激活Unit2Chapter5TheMovementofSubstancesAcrossCellMembraneSection1MembraneTransportProteinsandTransportAcrossCellMembraneofsmallmoleculesPoint1MasterthecharactersofcarrierproteinsandchannelproteinsPoint2Masterthetypesandfeaturesofsmallmoleculesacro

25、sscellmembraneSection2ATP-drivenPumpsandActiveTransportPoint1ApprehendandmasterthemechanismsandfunctionsoftheNa+-K+pumpandCa2+pumpPoint2MasterthefunctionsfoV-typepumpandF-typepumpPoint3MasterthefunctionsofABCsuperfamilyPoint4KnowtherelationsbetweenionstransportandmembranepotentialSection3Endocytosis

26、andExocytosisPoint1ApprehendandmastertheprocessandfunctionsofphagocytosisPoint2ApprehendandmastertheprocessandfeaturesofclathrindependentendocytosisPoint3KnowothertypesofpinocytosisPoint4Apprehendandmastertheregulationsofendocytosistosignaling知识单元三第六章线粒体和叶绿体（2学时）第一节线粒体与氧化磷酸化知识点一、理解并掌握线粒体的形态与动态变化知识点二

27、、掌握线粒体的超微结构及化学组成与定位知识点三、理解并掌握线粒体能量转换知识点四、了解线粒体与疾病第二节叶绿体与光合作用（自学）知识点一、了解叶绿体的形态、大小和数目知识点二、了解叶绿体的结构和化学组成知识点三、理解并掌握叶绿体的主要功能光合作用第三节线粒体和叶绿体的半自主性及其起源知识点一、了解线粒体和叶绿体的DNA知识点二、理解线粒体和叶绿体的蛋白质合成知识点三、理解线粒体和叶绿体基因组与细胞核的关系知识点四、了解线粒体和叶绿体的增殖与起源Unit3Chapter6MitochondriaandChloroplastSection1MitochondrionandOxidativePhos

28、phorylationPoint1ApprehendandmasterthemorphologyanddynamicsofmitochondrionPoint2KnowthechemicalcompositionandlocalizationofenzymesinmitochondrionPoint3ApprehendandmasterfunctionsofmitochondrionforenergyconversionPoint4KnowmitochondrionanddiseasesSection2ChloroplastandPhotosynthesis（Self-study）Point1

29、Knowthemodalities,sizesandnumberofchloroplastPoint2KnowstructureandchemicalcompositionofchloroplastPoint3ApprehendandmastermainfunctionofchloroplastphotosynthesisSection3MitochondrionandChloroplastareSemiautonomousOrganelles，andOriginPoint1KnowmitochondrionandchloroplastDNAPoint2Apprehendtheproteins

30、ynthesisofmitochondrionandchloroplastPoint3Apprehendtherelationbetweenmt/chDNAandnucleargenomePoint4Knowproliferationandoriginofmitochondrionandchloroplast知识单元四第七章细胞质基质与内膜系统（3学时）第一节细胞质基质及其功能知识点一、理解细胞质基质的含义知识点二、掌握细胞质基质的功能第二节细胞内膜系统及其功能知识点一、理解掌握内质网蛋白质合成、修饰加工、应激反应知识点二、理解并掌握高尔基体的极性特征、分泌活动、蛋白质修饰水解等知识点三、理解

31、并掌握溶酶体类型、功能、发生以及疾病知识点四、了解过氧化物酶体与溶酶体的区别、功能与发生知识单元5第八章蛋白质分选与膜泡运输（3学时）第一节细胞内蛋白质分选知识点一、理解并掌握信号假说与蛋白质分选信号知识点二、理解并掌握蛋白质分选途径与类型知识点三、了解线粒体、叶绿体和过氧化物酶体蛋白质分选第二节细胞内膜泡运输知识点一、理解并掌握内膜系统在结构、功能上的相互关系知识点二、了解包被蛋白种类知识点三、理解三种包被蛋白参与的包被小泡的装备与运输过程知识点四、了解膜泡的转运、锚定与融合知识点五、了解细胞结构体系的组装Unit4Chapter7CytosolandEndomembraneSystemSe

32、ction1CytoplasmicMatrix（Cytosol）anditsfunctionsPoint1ApprehendthemeaningofcytoplasmicmatrixPoint2MasterfunctionsofcytoplasmicmatrixSection2EndomembraneSystemandItsFunctionPoint1Apprehendandmasterproteinsynthesis,modificationandstressresponseinERPoint2ApprehendandmasterthepolarityofGolgicomplexandits

33、rolesinproteinsecretion,modificationandproteolysisPoint3Apprehendandmasterlysosome'stypes,functions,biogenesis,anditsroleindiseasesPoint4Knowthedifferenceoflysosomeandperoxisome,andthefunctionsandbiogenesisofperoxisomeUnit5Chapter8ProteinSortingandVesicularTransportingSection1ProteinSortingPoint

34、1ApprehendandmastersignalhypothesisandsortingsignalPoint2ApprehendandmasterpathwaysandtypesofproteinsortingPoint3Knowproteinsortingintomitochondrion,chloroplast,andperoxisomeSection2VesicularTransportingintheCellPoint1ApprehendandmasterrelationsofstructureandfunctionforendomembraneorganellesPoint2Kn

35、owthetypesofcoatingproteinsPoint3Apprehendtherolesof3typesofcoatingproteinsinvesicularassemblyandtransportingPoint4Knowtheprocessofvesiculartransporting,dockingandfusionPoint5Knowtheassemblyofsubcellularstructures知识单元6第九章细胞信号转导（3学时）第一节细胞信号转导概述知识点一、了解细胞通信类型知识点二、掌握信号分子与受体类型知识点三、理解并掌握信号转到特征第二节细胞内受体介导的信

36、号转递知识点一、了解核受体及其作用知识点二、掌握NO受体信号通路第三节、G蛋白偶联受体介导的信号传递知识点一、理解并掌握G蛋白偶联受体的结构与激活知识点二、理解G蛋白偶联受体介导的信号通路第四节酶联受体介导的信号传递知识点一、理解受体酪氨酸激酶及RKT-Ras信号通路知识点二、理解PI3K-PKB信号通路知识点三、了解其他类型信号通路第五节其他细胞表面受体介导的信号通路知识点一、了解Wnt-beta-catenin信号通路知识点二、了解Hedgehog受体介导的信号通路知识点三、了解NF-kB信号通路知识点四、了解Notch信号通路知识点五、了解整联蛋白介导的信号通路第六节细胞信号转导的整合与

37、控制知识点一、理解并掌握细胞的应答反应特征知识点二、理解蛋白激酶的网络整合信息知识点三、理解并掌握信号的控制：受体脱敏与下调Unit6Chapter9CellSignalTransductionSection1OverviewofCellSignalingPoint1KnowtypesofcellsignalingPoint2MastertypesofsignalmoleculesandreceptorsPoint3ApprehendandmastercharacteristicsofsignaltransductionSection2SignalTransductionbyIntracell

38、ularReceptorsPoint1KnownuclearreceptorsandfunctionsPoint2MastersignaltransductionbyNOSection3SignalTransductionbyGProtein-CoupledReceptorsPoint1ApprehendandmasterthedomainandactivationofGprotein-coupledreceptorsPoint2ApprehendsignalingpathwaysmediatedbyGprotein-coupledreceptorsSection4SignalTransduc

39、tionbyEnzyme-coupledReceptorsPoint1ApprehendthereceptortyrosinekinaseandRTK-RassignalpathwayPoint2ApprehendsignalpathwaymediatedbyPI3K-PKB(Akt)Point3KnowothertypesofsignalpathwaysSection5SignalPathwaysbyOtherReceptorsonCellSurfacePoint1KnowtheWnt-beta-cateninsignalingpathwayPoint2KnowtheHedghogsigna

40、lingpathwayPoint3KnowtheNF-kBsignalingpathwayPoint4KnowtheNotchsignalingpathwayPoint5KnowsignalingpathwaymediatedbyintegrinsSection6TheConvergenceandRegulationofSignalTransductionPoint1ApprehendandmasterthefeaturesofsignalresponsesPoint2ApprehendtheConvergenceofthecross-talkofsignalingPoint3Apprehen

41、dthedesensitizationanddown-regulationofreceptors知识块三细胞骨架系统LearningBlock3TheCytoskeletonSystem知识单元一第十章细胞骨架（4学时）第一节微丝与细胞运动知识点一、理解并掌握微丝装配动态性知识点二、理解并掌握微丝结合蛋白类型、作用以及与细胞运动知识点三、掌握微丝的分子马达种类知识点四、理解并掌握肌肉收缩的分子机制第二节微管及其功能知识点一、理解并掌握微管的极性与装配动态知识点二、掌握微观组织者中心类型知识点三、理解并掌握微管结合蛋白对微管组织形式的调节作用知识点四、理解并掌握微管马达蛋白及其对胞内物质运输的作

42、用知识点五、掌握纤毛和鞭毛的结构、功能及运动机制第三讲中间丝知识点一、了解中间丝的类型知识点二、掌握中间丝的装配特点及组织分布特点知识点三、掌握中间丝功能Unit1Chapter10TheCytoskeletonSection1MicrofilamentsandCellMotilityPoint1ApprehendandmasterthedynamicassemblyofmicrofilamentsPoint2Apprehendandmasterthetypesandfunctionsofmicrofilament-associatedproteinsandcellmotilityPoint3

43、MasterthetypesofmicrofilamentassociatedmotorproteinsPoint4ApprehendandmastermolecularmechanismofmusclecontractionSection2MicrotubulesPoint1ApprehendandmasterthepolarityanddynamicpropertiesofmicrotubulesPoint2Mastertypesofmicrotubule-organizingcentersPoint3Apprehendandmastertheregulationofmicrotubule

44、sbymicrotubule-associatedproteinsPoint4ApprehendandmastermotorproteinsandintracellulartransportPoint5Masterthestructure,functionandmotilitymechanismofciliaandflagellaSection3IntermediateFilamentsPoint1MastertypesofintermediatefilamentsPoint2Mastertheassemblyanddisassemblyandtissuedistributionofinter

45、mediatefilamentsPoint3Masterthefunctionofintermediatefilaments知识块四遗传信息荷载与表达系统LearningBlock4GeneticInformationCarrierandExpressionSystem知识单元一第十一章细胞核与染色质（5学时）第一节核被膜知识点一、掌握核被膜结构与装配动态知识点二、掌握并理解核孔复合体功能知识点三、掌握核纤层功能第二节染色质知识点一、掌握染色质的概念及化学组成知识点二、掌握染色质的基本结构单位核小体知识点三、理解染色质包装的结构模型知识点四、掌握常染色质和异染色质第三节染色质的复制与表达知识点

46、一、了解染色质复制与修复知识点二、了解染色质的激活与失活知识点三、了解染色质与基因表达调控知识点四、了解染色质与表观遗传第四节染色体知识点一、了解中期染色体的形态结构知识点二、理解并掌握染色体DNA的三种功能元件知识点三、了解核型与染色体显带知识点四、理解巨大染色体的形成原因和作用第五节核仁与核体知识点一、掌握核仁的超微结构知识点二、理解并掌握核仁的功能知识点三、了解核仁的周期知识点四、了解核体Unit1Chapter11NucleusandChromatinsSection1NuclearEnvelopePoint1Masterstructureanddynamicsofnuclearenv

47、elopePoint2ApprehendandmasterfunctionsofnuclearporecomplexPoint3MasterthefunctionofnuclearlaminaSection2ChromatinPoint1MasterconceptandchemicalcompositionofchromosomePoint2MasterthebasicstructureunitofchromosomenucleosomePoint3ApprehendstructuralmodelofchromosomepackagePoint4Masterofeuchromatinandhe

48、terochromatinSection3ReplicationandExpressionofChromatinsPoint1KnowthereplicationandrepairmenofchromatinsPoint2KnowtheactivationandinactivationofchromatinsPoint3KnowtheregulationofgeneexpressionbychromatinsSection4ChromosomesPoint1KnowmodalstructureofmetaphasechromosomePoint2Apprehendandmaster3funct

49、ionalelementsofchromosomeDNAPoint3KnowkaryotypeandbandingPoint4ApprehendcauseandfunctionofgiantchromosomeSection5TheNucleolusandTheNuclearBodyPoint1MasterultrastructureofnucleolusPoint2ApprehendandmasterfunctionsofnucleolusPoint3KnownucleolarcyclePoint4Knowthenuclearbodies知识单元二第十二章核糖体（自学内容1学时）第一节核糖体

50、的类型与结构知识点一、掌握核糖体的基本类型与成分知识点二、掌握核糖体的结构知识点三、掌握核糖体蛋白质与rRNA的功能第二节多聚核糖体与蛋白质的合成知识点一、了解多聚核糖体知识点二、了解蛋白质的合成知识点三、了解RNA在生命起源中的地位Unit2Chapter11Ribosome（Self-study）Section1TypesandStructureofRibosomePoint1MasterbasictypesandcompositionofribosomePoint2MasterstructureofribosomePoint3Masterfunctionsribosomalprotein

51、sandrRNASection2PolyribosomeandProteinSynthesisPoint1KnowpolyribosomePoint2MasterproteinsynthesisPoint3KnowpositionofRNAinlifeorigin知识块五细胞增殖、癌变、分化、衰老、凋亡LearningBlock5CellProliferation,DifferentiationandCellSenescenceandApoptosis知识单元一第十三章细胞周期与细胞分裂（3学时）第一节细胞周期知识点一、掌握细胞周期概念和周期时相事件知识点二、掌握细胞周期同步化的方法原理知识点

52、三、掌握早期胚胎细胞的细胞周期第二节细胞分裂知识点一、理解并掌握有丝分裂各时期的重要事件及其结构装置知识点二、理解并掌握动物细胞胞质分裂分子调控机制知识点三、理解减数分裂过程以及减数分裂意义知识点四、掌握减数分裂的特殊结构及其变化Unit1Chapter13CellCycleandCellDivisionSection1CellCyclePoint1MastercellcycleconceptsandcyclingphraseeventsPoint2MasterprincipleofcellcyclesynchronizationmethodPoint3Masterthecellcycleof

53、theearlyfrogembryoSection2CellDivisionPoint1ApprehendandmastereventsandapparatusesincellcyclephasesPoint2ApprehendandmasterthemechanismofanimalcytokinesisPoint3ApprehendtheprocessandsignificancesofmeiosisPoint4Masterthespecialapparatusanditsdynamicofmeiosis知识单元二第十四章细胞增殖调控与癌细胞（3学时）第一节细胞增殖调控知识点一、理解MPF

54、的发现及其作用知识点二、理解P34cdc2激酶的发现及其与MPF的关系知识点三、掌握周期蛋白的作用知识点四、掌握CDK激酶和CDK激酶抑制物知识点五、理解并掌握细胞周期如何正常有序的运转及调控知识点六、了解其他内在和外在因素在细胞周期调控中的作用第二节癌细胞知识点一、掌握癌细胞的基本特征知识点二、掌握癌基因与抑癌基因对细胞癌变的作用知识点三、理解肿瘤发生是基因突变积累的结果知识点四、理解并掌握肿瘤干细胞Unit2Chapter14CellCycleControlandCancerCellsSection1CellCycleControlPoint1Apprehendthediscoveryan

55、dfunctionsofMPFPoint2ApprehenddiscoveryofP34cdc2kinaseanditsrelationwithMPFPoint3MasterfunctionsofcyclicproteinPoint4MasterCDKkinaseandCDKinhibitorsPoint5MasterandapprehendhowcellcyclefunctionproperlyanditsregulationPoint6KnowotherinnerandouterfactorsincellcycleregulationSection2CancerCellPoint1Mast

56、erbasicfeaturesofcancercellsPoint2Masterrelationsbetweencancersandoncogenesandtumor-suppressorgenesPoint3ApprehendcancersresultsfromaccumulationofgenemutationPoint4Apprehendandmastercancerstemcells知识单元三第十五章细胞分化与胚胎发育（3学时）第一节细胞分化知识点一、掌握细胞分化的基本概念以及去分化和再生知识点二、理解并掌握细胞的全能性与多能干细胞及诱导多能干细胞知识点三、了解影响细胞分化的因素第二节

57、胚胎发育中的细胞分化知识点一、了解生殖细胞的分化知识点二、了解早期胚胎发育过程中的细胞分化Unit3Chapter15CellDifferentiationandEmbryonicDevelopmentSection1CellDifferentiationPoint1Masterbasicconceptsofcelldifferentiation,dedifferentiationandre-differentiationPoint2KnowfactorsinfluencingcelldifferentiationPoint3ApprehendandmastertotipotencyandstemcellPoint4KnowcelldifferentiationandembryodevelopmentSection2CellDifferentiationinEmbryonicDevelopmentPoint1KnowthedifferentiationofgermcellsPoint2Knowthecelldifferentiationduringearlyemb