ISX-9-Isoxazole-9-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEISX-9Cat.No.:HY-12323CASNo.:832115-62-5Synonyms:Isoxazole9分⼦式:C₁₁H₁₀N₂O₂S分⼦量:234.27作⽤靶点:CalciumChannel作⽤通路:MembraneTransporter/IonChannel;NeuronalSignaling储存⽅式:Powder-20°C3years4°C2yearsInsolvent-80°C6months-20°C1month溶解性数据体外实验DMSO:≥100mg/mL(426.86mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM4.2686mL21.3429mL42.6858mL5mM0.8537mL4.2686mL8.5372mL10mM0.4269mL2.1343mL4.2686mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(10.67mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:2.5mg/mL(10.67mM);Suspendedsolution;Needultrasonic3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(10.67mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性ISX-9(Isoxazole9)⼀种神经⼲细胞分化的有效诱导剂。ISX-9通过电压门控的Ca2+通道和NMDA受体来激活Ca2+流⼊，并增加NeuroD表达。ISX-9还可诱导Notch激活的⼼外膜衍⽣细胞(NEC)的⼼肌分化。体外研究ISX-9promotesneurogenesisinvivo,enhancingtheproliferationanddifferentiationofhippocampalsubgranularzone(SGZ)neuroblasts,andthedendriticarborizationofadult-generateddentategyrusneurons.At2.5-20μM,ISX-9hasbeenshowntodose-dependentlytriggerneurogenesisandblockgliogenesisinadultrathippocampalstemcellsthroughacalcium-activatedsignalingpathwaydependentonmyocyte-enhancerfactor2-dependentgeneexpression[1].MolecularexplorationofISX-9-inducedregulationofneurogenesis(viaFACSandmicroarrayofSGZstemandprogenitorcells)suggestedtheinvolvementofthemyocyte-enhancerfamilyofproteins(Mef2)[1].体内研究ISX-9(20mg/kg;for12days;mice)treatmentimproveshippocampalfunction.ISX-9enhancesspatialmemoryabilityintheMorriswatermazetest.ISX-9enhanceshippocampalneurogenesisandmemoryinvivo,anditseffectsarereliantonMef2[1].户使⽤本产品发表的科研⽂献•AdvSci(Weinh).2022Mar3;e2104682.•StemCellReports.2017Mar14;8(3):538-547.•ACSChemNeurosci.2018Dec19;9(12):3175-3185.•BrainScienceAdvances.2022Feb.•MaterialsTodayChemistry12(2019)78e84Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].PetrikD,etal.Functionalandmechanisticexplorationofanadultneurogenesis-promotingsmallmolecule.FASEBJ.2012Aug;26(8):3148-3162.[2].JayWSchneider,etal.Small-moleculeactivationofneuronalcellfate.NatChemBiol.2008Jul;4(7):408-10.[3].JamieLRussell,etal.Targetingnativeadultheartprogenitorswithcardiogenicsmallmolecules.ACSChemBiol.2012Jun15;7(6):1067-76.McePdfHeight2/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemECaution:Producthasnotbeenfullyvalidate