




已阅读5页,还剩15页未读, 继续免费阅读
版权说明:本文档由用户提供并上传,收益归属内容提供方,若内容存在侵权,请进行举报或认领
文档简介
BSISO162972013MILKBACTERIALCOUNTPROTOCOLFORTHEEVALUATIONOFALTERNATIVEMETHODSLAITDNOMBREMENTBACTRIENPROTOCOLEPOURLVALUATIONDESMTHODESALTERNATIVESINTERNATIONALSTANDARDFIRSTEDITION20130601ISOANDIDF2013REFERENCENUMBERSISO162972013EIDF1612013EISO16297IDF161LICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013EIDF1612013EIIISOANDIDF2013ALLRIGHTSRESERVEDCOPYRIGHTPROTECTEDDOCUMENTISO/IDF2013ALLRIGHTSRESERVEDUNLESSOTHERWISESPECIFIED,NOPARTOFTHISPUBLICATIONMAYBEREPRODUCEDORUTILIZEDOTHERWISEINANYFORMORBYANYMEANS,ELECTRONICORMECHANICAL,INCLUDINGPHOTOCOPYING,ORPOSTINGONTHEINTERNETORANINTRANET,WITHOUTPRIORWRITTENPERMISSIONPERMISSIONCANBEREQUESTEDFROMEITHERISOORIDFATTHERESPECTIVEADDRESSBELOWORISOSMEMBERBODYINTHECOUNTRYOFTHEREQUESTERISOCOPYRIGHTOFFICEINTERNATIONALDAIRYFEDERATIONCASEPOSTALE56CH1211GENEVA20SILVERBUILDINGBDAUGUSTEREYERS70/BB1030BRUSSELSTEL41227490111TEL3227339888FAX41227490947FAX3227330413EMAILCOPYRIGHTISOORGEMAILINFOFILIDFORGWEBWWWISOORGWEBWWWFILIDFORGPUBLISHEDINSWITZERLANDLICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013EIDF1612013EISOANDIDF2013ALLRIGHTSRESERVEDIIICONTENTSPAGEFOREWORDIVINTRODUCTIONVI1SCOPE12NORMATIVEREFERENCES13TERMSANDDEFINITIONS14TRANSFORMATIONOFRESULTS15ATTRIBUTESOFTHEALTERNATIVEMETHOD251DESCRIPTIONOFTHEMETHODTOBEEVALUATED252MEASURINGRANGE253CARRYOVER354STABILITY555PRECISION56THEALTERNATIVEMETHODASANESTIMATEOFTHEREFERENCEMETHOD661EVALUATIONOFFACTORSAFFECTINGTHEESTIMATION662MEASUREMENTPROTOCOL763CALCULATIONS764ATTRIBUTESOFTHEALTERNATIVEMETHODEXPRESSEDINUNITSOFTHEREFERENCEMETHOD107RATINGOFTHEELABORATEDATTRIBUTES10ANNEXAINFORMATIVEEXPRESSIONOFPRECISIONPARAMETERS11BIBLIOGRAPHY13LICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013EFOREWORDISOTHEINTERNATIONALORGANIZATIONFORSTANDARDIZATIONISAWORLDWIDEFEDERATIONOFNATIONALSTANDARDSBODIESISOMEMBERBODIESTHEWORKOFPREPARINGINTERNATIONALSTANDARDSISNORMALLYCARRIEDOUTTHROUGHISOTECHNICALCOMMITTEESEACHMEMBERBODYINTERESTEDINASUBJECTFORWHICHATECHNICALCOMMITTEEHASBEENESTABLISHEDHASTHERIGHTTOBEREPRESENTEDONTHATCOMMITTEEINTERNATIONALORGANIZATIONS,GOVERNMENTALANDNONGOVERNMENTAL,INLIAISONWITHISO,ALSOTAKEPARTINTHEWORKISOCOLLABORATESCLOSELYWITHTHEINTERNATIONALELECTROTECHNICALCOMMISSIONIECONALLMATTERSOFELECTROTECHNICALSTANDARDIZATIONINTERNATIONALSTANDARDSAREDRAFTEDINACCORDANCEWITHTHERULESGIVENINTHEISO/IECDIRECTIVES,PART2THEMAINTASKOFTECHNICALCOMMITTEESISTOPREPAREINTERNATIONALSTANDARDSDRAFTINTERNATIONALSTANDARDSADOPTEDBYTHETECHNICALCOMMITTEESARECIRCULATEDTOTHEMEMBERBODIESFORVOTINGPUBLICATIONASANINTERNATIONALSTANDARDREQUIRESAPPROVALBYATLEAST75OFTHEMEMBERBODIESCASTINGAVOTEATTENTIONISDRAWNTOTHEPOSSIBILITYTHATSOMEOFTHEELEMENTSOFTHISDOCUMENTMAYBETHESUBJECTOFPATENTRIGHTSISOSHALLNOTBEHELDRESPONSIBLEFORIDENTIFYINGANYORALLSUCHPATENTRIGHTSISO16297|IDF161WASPREPAREDBYTECHNICALCOMMITTEEISO/TC34,FOODPRODUCTS,SUBCOMMITTEESC5,MILKANDMILKPRODUCTSANDTHEINTERNATIONALDAIRYFEDERATIONIDFITISBEINGPUBLISHEDJOINTLYBYISOANDIDFIDF1612013EIVISOANDIDF2013ALLRIGHTSRESERVEDLICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013EFOREWORDIDFTHEINTERNATIONALDAIRYFEDERATIONISANONPROFITORGANIZATIONREPRESENTINGTHEDAIRYSECTORWORLDWIDEIDFMEMBERSHIPCOMPRISESNATIONALCOMMITTEESINEVERYMEMBERCOUNTRYASWELLASREGIONALDAIRYASSOCIATIONSHAVINGSIGNEDAFORMALAGREEMENTONCOOPERATIONWITHIDFALLMEMBERSOFIDFHAVETHERIGHTTOBEREPRESENTEDATTHEIDFSTANDINGCOMMITTEESCARRYINGOUTTHETECHNICALWORKIDFCOLLABORATESWITHISOINTHEDEVELOPMENTOFSTANDARDMETHODSOFANALYSISANDSAMPLINGFORMILKANDMILKPRODUCTSTHEMAINTASKOFSTANDINGCOMMITTEESISTOPREPAREINTERNATIONALSTANDARDSDRAFTINTERNATIONALSTANDARDSADOPTEDBYTHESTANDINGCOMMITTEESARECIRCULATEDTOTHENATIONALCOMMITTEESFORENDORSEMENTPRIORTOPUBLICATIONASANINTERNATIONALSTANDARDPUBLICATIONASANINTERNATIONALSTANDARDREQUIRESAPPROVALBYATLEAST50OFIDFNATIONALCOMMITTEESCASTINGAVOTEATTENTIONISDRAWNTOTHEPOSSIBILITYTHATSOMEOFTHEELEMENTSOFTHISDOCUMENTMAYBETHESUBJECTOFPATENTRIGHTSIDFSHALLNOTBEHELDRESPONSIBLEFORIDENTIFYINGANYORALLSUCHPATENTRIGHTSISO16297|IDF161WASPREPAREDBYTHEINTERNATIONALDAIRYFEDERATIONIDFANDTECHNICALCOMMITTEEISO/TC34,FOODPRODUCTS,SUBCOMMITTEESC5,MILKANDMILKPRODUCTSITISBEINGPUBLISHEDJOINTLYBYIDFANDISOALLWORKWASCARRIEDOUTBYJOINTISOIDFPROJECTGROUPS07OFTHESTANDINGCOMMITTEEONSTATISTICSANDAUTOMATIONUNDERTHEAEGISOFITSPROJECTLEADER,MRSIANDERSSONSETHISFIRSTEDITIONOFISO16297|IDF161CANCELSANDREPLACESIDF161A1995,WHICHHASBEENTECHNICALLYREVISEDIDF1612013EISOANDIDF2013ALLRIGHTSRESERVEDVLICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013EINTRODUCTIONANYQUANTITATIVEMEASUREMENTINMICROBIOLOGYSHOULDCONSIDERTHATTHEREISAREQUIREMENTFORTHEMICROBIOLOGICALSTATEINASAMPLETOBEREGARDEDASONEPOINTWITHINTHECOORDINATESOFAMULTIDIMENSIONALSYSTEM,WHICHISTOBEPROJECTEDONTOTHEONEDIMENSIONALSCALEOFTHEMETHODAPPLIED,IEPLATECOUNT,FLOWCYTOMETRYASPECTSSUCHASFLORATYPESANDNUMBERSOFMICROORGANISMSANDTHEIRDISTRIBUTION,GROWTHPHASE,SUBLETHALDAMAGE,METABOLICACTIVITY,ANDHISTORY,INFLUENCETOAGREATERORLESSEREXTENTANYPARAMETERTHATISMEASUREDITISEVIDENTTHATANYPROJECTIONOFANNDIMENSIONALSITUATIONONTOANONEDIMENSIONALSCALEISBOUNDTOPROVIDEAPICTUREOFTHEREALSITUATIONTHATISRATHERRESTRICTEDINTHISRESPECTONEHASTOBOWTOTHEINEVITABLE,REGARDLESSOFWHICHMETHODOFMEASUREMENTISPREFERREDTHETERMREFERENCEOROFFICIALORANCHORMETHODINTHISINTERNATIONALSTANDARDMEANSAMETHODINTERNATIONALLYRECOGNIZEDBYEXPERTS,USEDINLEGISLATIONORBYAGREEMENTBETWEENTHEPARTIESTHEREAREREQUIREMENTSFOREVALUATIONOFANALTERNATIVEMETHODTOREFERTOTHEREFERENCEMETHODANDTOBEBASEDONTHEEXAMINATIONOFSUITABLESAMPLESFORITSINTENDEDUSEIDF1612013EVIISOANDIDF2013ALLRIGHTSRESERVEDLICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013INTERNATIONALSTANDARDISO162972013EIDF1612013EMILKBACTERIALCOUNTPROTOCOLFORTHEEVALUATIONOFALTERNATIVEMETHODS1SCOPETHISINTERNATIONALSTANDARDGIVESGUIDELINESFORTHEEVALUATIONOFINSTRUMENTALALTERNATIVEMETHODSFORTOTALBACTERIALCOUNTINRAWMILKFROMANIMALSOFDIFFERENTSPECIESNOTETHEDOCUMENTISCONSIDEREDCOMPLEMENTARYTOISO16140ANDISO8196|IDF128SEECLAUSE2ANDREFERENCE12NORMATIVEREFERENCESTHEFOLLOWINGREFERENCEDDOCUMENTSAREINDISPENSABLEFORTHEAPPLICATIONOFTHISDOCUMENTFORDATEDREFERENCES,ONLYTHEEDITIONCITEDAPPLIESFORUNDATEDREFERENCES,THELATESTEDITIONOFTHEREFERENCEDDOCUMENTINCLUDINGANYAMENDMENTSAPPLIESISO57251,ACCURACYTRUENESSANDPRECISIONOFMEASUREMENTMETHODSANDRESULTSPART1GENERALPRINCIPLESANDDEFINITIONSISO57252,ACCURACYTRUENESSANDPRECISIONOFMEASUREMENTMETHODSANDRESULTSPART2BASICMETHODFORTHEDETERMINATIONOFREPEATABILITYANDREPRODUCIBILITYOFASTANDARDMEASUREMENTMETHODISO81961|IDF1281,MILKDEFINITIONANDEVALUATIONOFTHEOVERALLACCURACYOFALTERNATIVEMETHODSOFMILKANALYSISPART1ANALYTICALATTRIBUTESOFALTERNATIVEMETHODSISO81962|IDF1282,MILKDEFINITIONANDEVALUATIONOFTHEOVERALLACCURACYOFALTERNATIVEMETHODSOFMILKANALYSISPART2CALIBRATIONANDQUALITYCONTROLINTHEDAIRYLABORATORYISO161401,MICROBIOLOGYOFFOODANDANIMALFEEDMETHODVALIDATIONPART1VOCABULARYISO161402,MICROBIOLOGYOFFOODANDANIMALFEEDMETHODVALIDATIONPART2PROTOCOLFORTHEVALIDATIONOFALTERNATIVEPROPRIETARYMETHODSAGAINSTAREFERENCEMETHODISO21187|IDF1962004,MILKQUANTITATIVEDETERMINATIONOFBACTERIOLOGICALQUALITYGUIDANCEFORESTABLISHINGANDVERIFYINGACONVERSIONRELATIONSHIPBETWEENROUTINEMETHODRESULTSANDANCHORMETHODRESULTS3TERMSANDDEFINITIONSFORTHEPURPOSESOFTHISDOCUMENT,THETERMSANDDEFINITIONSGIVENINISO81961|IDF1281ANDISO81962|IDF1282APPLYFORTHEDEFINITIONSOFPRECISION,REPEATABILITYANDREPRODUCIBILITY,SEEISO57251,ISO57252,ISO81961|IDF1281,ANDISO1614014TRANSFORMATIONOFRESULTSAPREREQUISITEFORSTATISTICSMOSTCOMMONINTHEEVALUATIONOFMEASURINGMETHODSISTHEAPPROXIMATIONOFANORMALDISTRIBUTIONOFTHEDATATHEEXPONENTIALMULTIPLICATIONOFMICROORGANISMSUSUALLYLEADSTOARIGHTTAILEDDISTRIBUTIONOFQUANTITATIVEMICROBIOLOGICALPARAMETERSTHUS,INGENERAL,TRANSFORMATIONOFTHERAWDATAISNECESSARYFORAPPROXIMATIONOFNORMALITYTHISISUSUALLYACOMMONLOGARITHMICTRANSFORMATIONORASQUAREROOTTRANSFORMATIONFORLOWBACTERIALEVELSTHEMOSTAPPROPRIATETRANSFORMATIONCANBECHECKEDBYCOMPARINGHISTOGRAMSALLSTATISTICSARETHENCOMPUTEDFROMTHEISOANDIDF2013ALLRIGHTSRESERVED1LICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013ETRANSFORMEDDATA,UNLESSSPECIFIEDOTHERWISE,ANDONLYTHEFINALRESULTSARERETRANSFORMEDTOGIVEAMOREEXPRESSIVEIDEAOFTHESITUATIONTOTHEUSERSEEALSOANNEXA5ATTRIBUTESOFTHEALTERNATIVEMETHODNOTETHEPARAMETERSOUTLINEDINTHISCLAUSEDONOTNEEDTOBEEVALUATEDCOMPLETELYFOREVERYALTERNATIVEMETHODFOREXAMPLE,THEMEASURINGRANGESEE52OFTHEPLATELOOPMETHODISDETERMINEDBYTHELOOPSUSED51DESCRIPTIONOFTHEMETHODTOBEEVALUATED511DESCRIPTIONTHEDESCRIPTIONOFTHEMETHODUNDERSTUDYSHALLBEINLINEWITHTHECHECKLISTIN512MOSTOFTHEINFORMATIONISFOUNDINTHESPECIFICATIONOFTHEMETHODGIVENBYTHERESPONSIBLESUPPLIERORANYOTHERSOURCEAUTHOROFTHETECHNIQUESPECIFIED512CHECKLISTAPRINCIPLEOFTHEMETHODBPARAMETERORUNITCTECHNICALDESIGNOFTHEMEASUREMENTPROCEDUREDFIELDOFAPPLICATION1PURPOSEEGRESEARCH,SCREENING,MILKGRADING2MATRIXEGRAWMILKFROMCOWSESUPPLIERSOFINSTRUMENT,REAGENTS,STANDARDSFSPECIFICATIONOFTHEMETHODGIVENBYTHEPRODUCERORTHEAUTHOR1PREREQUISITESFORSAMPLINGOFTENCOMPAREDTOTHESITUATIONOFFATANALYSIS2POSSIBILITIESFORSAMPLEPRESERVATIONREAGENTS,STORAGECONDITIONS3QUANTITATIVEUNITSMETHODUNDERSTUDYORREFERENCEMETHODANDQUALITATIVETHEKINDOFMICROORGANISMSCOVEREDSPECTRUM4PRECISIONINUNITSOFTHEMETHODUNDERSTUDYORINREFERENCEMETHODUNITS5ACCURACYOFTHEESTIMATEINREFERENCEMETHODUNITS6SAMPLESPERHOUR7LISTOFREFERENCES52MEASURINGRANGE521LOWERLIMITOFQUANTIFICATIONTHELOWERLIMITOFQUANTIFICATIONISDEFINEDASTHEAVERAGEOFMILKWITHOUTBACTERIAPLUSTHENFOLDOFITSSTANDARDDEVIATIONGENERALLY,N10SEEALSOISO161402ANALYSEMILKWITHOUTBACTERIAORWITHAVERYLOWCONCENTRATIONOFBACTERIATRANSFORMDATABYCALCULATINGSQUAREROOTFROMEACHRESULTCALCULATETHEMEAN,X,ANDTHESTANDARDDEVIATION,S,OFTHETRANSFORMEDRESULTSCALCULATETHELOWERLIMITOFQUANTIFICATIONASXNSIDF1612013E2ISOANDIDF2013ALLRIGHTSRESERVEDLICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013E522UPPERLIMITOFQUANTIFICATIONTHEUPPERLIMITOFQUANTIFICATIONISDETERMINEDBYTHEHIGHESTPOSSIBLEREADINGOFTHEMETHODORBYMETHODOLOGICALLIMITATIONS,EGCOINCIDENCEEFFECTS,INACCURACYINTHEUPPERMEASURINGRANGE,CLOGGINGOFFILTERSCOINCIDENCEISWHENTWOORMOREELEMENTSOFTHEMEASURANDAREDETECTEDSIMULTANEOUSLYANDIDENTIFIEDASONLYONEUNITFOREXAMPLE,WITHFLOWCYTOMETRY,IFTWOBACTERIALCELLSPASSTHEDETECTORSIMULTANEOUSLY,THEYAREDETECTEDASONETHECOINCIDENCEEFFECTISHIGHERWITHHIGHERCONCENTRATIONSOFAMEASURANDUPPERLIMITOFQUANTIFICATIONISDETERMINEDASTHEHIGHESTCONCENTRATIONWHERETHEINSTRUMENTISSTILLLINEARACCORDINGTO523523LINEARITYOFTHEINSTRUMENTSIGNALTHERELATIONSHIPBETWEENTHEINSTRUMENTREADINGSANDTHEEXPECTEDVALUESSHALLBELINEARWITHINTHECONCERNEDRANGEOFBACTERIALCOUNTSDEVIATIONSFROMLINEARITYMAYSTEMFROMNONSPECIFICSIGNALSANDCOINCIDENCEEFFECTSALINEARITYCHECKISATFIRSTPERFORMEDVISUALLYUSINGAPPROPRIATEGRAPHSTOOBTAINANIMPRESSIONOFTHESHAPEOFTHERELATIONSHIPWHENEVERDEVIATIONFROMLINEARITYAPPEARSEVIDENT,AQUANTITATIVEPARAMETERISCALCULATEDTOINDICATEWHETHERTHEOBSERVEDTRENDISACCEPTABLEORNOTTOACHIEVETHIS,USEAHIGHBACTERIALCOUNTMILKDILUTEDSERIALLYWITHLOWBACTERIALCOUNTMILK,RESULTINGINASETOFATLEAST10SAMPLESCOVERINGTHECONCENTRATIONRANGEOFINTERESTMEASUREALLSAMPLESATLEASTFOURTIMESANDCALCULATETHEAVERAGERESULTFOREACHSAMPLETHISGIVESTHEMEASUREDVALUEPERSAMPLEUSETHEMEASUREDVALUESFORTHEHIGHCOUNTMILKANDTHELOWCOUNTMILKTOCALCULATEVALUESFORTHEINTERMEDIATESAMPLESFROMTHEAPPLIEDMIXINGRATIOSTHISRESULTSINANEXPECTEDVALUEFOREACHSAMPLETHENAPPLYLINEARREGRESSIONWITHTHEEXPECTEDVALUESPERSAMPLE,CE,ONTHEXAXISANDTHEMEASUREDVALUESPERSAMPLE,CMEAS,ONTHEYAXISCALCULATETHERESIDUALSC1ICMEAS,IACE,IBFROMTHEREGRESSIONPLOTTHERESIDUALSC1IONTHEYAXISVERSUSTHEEXPECTEDVALUES,CE,ONTHEXAXISAVISUALINSPECTIONOFTHEDATAPOINTSUSUALLYYIELDSSUFFICIENTINFORMATIONABOUTTHELINEARITYOFTHESIGNALANYOUTLYINGRESIDUALSHOULDLEADTODELETIONOFTHERELATEDRESULTANDTORENEWALOFTHECALCULATIONTHECURVINGCANBEEXPRESSEDBYTHERATIO,RL,USINGFORMULA1RCCCCLMAXMINMINMEAS,MAXMEAS,1001WHERECMAXISTHEVALUEOFTHEMAXIMUMRESIDUALFROMTHEREGRESSIONCMINISTHEVALUEOFTHEMINIMUMRESIDUALFROMTHEREGRESSIONCMEAS,MAXISTHEMEASUREDVALUEFORTHEHIGHCOUNTMILKCMEAS,MINISTHEMEASUREDVALUEFORTHELOWCOUNTMILKTHERATIO,RL,SHALLBELESSTHAN5NOTETOEVALUATELINEARITY,USETHERAWDATAEXPRESSEDINUNITSOFTHEROUTINEMETHODWITHOUTLOGARITHMICORANYOTHERTRANSFORMATION53CARRYOVERCARRYOVEREFFECTSCANOCCURINANALYTICALSYSTEMSTHATOPERATECONTINUOUSLYITDERIVESFROMTHETRANSFEROFACERTAINPORTIONOFSAMPLEMATERIALFROMONETESTSAMPLETOTHENEXTORFURTHERSAMPLESIDF1612013EISOANDIDF2013ALLRIGHTSRESERVED3LICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013EDUETOTHEDESIGNOFAMECHANIZEDPROCESSOFANALYSIS,NOTONLYTHENEXTSAMPLE,BUTALSOSAMPLESINALATERPOSITIONCANBEINFLUENCEDDUE,FOREXAMPLE,TOINCUBATIONWELLSWITHAPERIODICCIRCULATIONTHISEFFECTCANBETESTEDBYANALYSINGCONSECUTIVELYMILKWITHHIGHBACTERIALCOUNTANDBLANKSAMPLESCARRYOVERCAUSESANINCREASEOFBLANKSAMPLEVALUESCOMPAREDTONORMALBLANKSAMPLEVALUEVALUEOFBLANKSAMPLEANALYSEDAFTERANOTHERBLANKSAMPLETHECARRYOVERCANBEEXPRESSEDASPERCENTAGEOFTHECORRESPONDINGPRECEDINGMILKSAMPLEFOREVALUATIONOFCARRYOVER,THENUMBEROFSAMPLESANDTHEBACTERIALCOUNTOFTHEMILKSAMPLESSHOULDBEHIGHENOUGHTOESTIMATETHECARRYOVERWITHSUFFICIENTCERTAINTYTHESAMPLESSHOULDBEREPRESENTATIVEOFTHEROUTINESAMPLES,ESPECIALLYREGARDINGTHESTORAGETIMELONGERSTORAGETIMELEADINGTOHIGHERMILKVISCOSITYANDPOTENTIALLYHIGHERCARRYOVERONEWAYOFSETTINGUPTHETESTISDESCRIBEDINTHEEXAMPLEBELOWFORDETAILEDANDTHEORETICALASPECTSANDALTERNATIVESETUPSOFCARRYOVERESTIMATION,ITISREFERREDTOISO81963|IDF12831ASANEXAMPLE,ONEWAYTOESTIMATETHECARRYOVEREFFECTISTOANALYSEATLEAST10SETSOFSAMPLES,EACHSETCONTAININGONEMILKSAMPLEWITHVERYHIGHBACTERIALCOUNTFOLLOWEDBYTWOBLANKSAMPLESBLANKSAMPLESCOULDBEWATERORMILKWITHNEGLIGIBLEBACTERIALCOUNTMILK,BLANK1,BLANK21,MILK,BLANK1,BLANK22MILK,BLANK1,BLANK2NTHERELATIVECARRYOVER,COR,EXPRESSEDASAPERCENTAGE,CANBECALCULATEDFOREACHSAMPLESETANDTHENAVERAGEDCORBBSIIIICCC121002CORCORIIN3WHERECORIISTHERELATIVECARRYOVERINTHEITHSAMPLESETCIB1ISTHERESULTOFTHEFIRSTBLANKSAMPLEINTHEITHSAMPLESETCIB2ISTHERESULTOFTHESECONDBLANKSAMPLEINTHEITHSAMPLESETCISISTHERESULTOFTHEMILKSAMPLEINTHEITHSAMPLESETNISTHENUMBEROFSAMPLESETSEVENAVERYLOWCARRYOVEREFFECTCANBERELEVANTIFTHECORRESPONDINGPRECEDINGSAMPLEHASAVERYHIGHLEVELINCOMPARISONTOTHENEXTONEITCANEVENCAUSETHERESULTOFTHENEXTSAMPLETOEXCEEDAGIVENLIMITCARRYOVERSHALLBEBELOW1ANEXAMPLEOFCARRYOVEREFFECTISGIVENINFIGURE1THERESULTSOFBLANKSOLUTIONSANALYSEDIMMEDIATELYAFTERHIGHCOUNTSAMPLESAREPLOTTEDAGAINSTTHERESULTSOFTHECORRESPONDINGPRECEDINGMILKSAMPLESFROMTHEGRAPH,THEMEASURINGLEVELOFPRECEDINGMILKSAMPLESWHICHCANLEADTOANINCREASEOFTHEBLANKVALUESABOVETHEACCEPTEDLEVELCANBEDERIVEDTHERELATIONBETWEENSAMPLEANDBLANKVALUESCANBEAPPROXIMATEDBYAFUNCTION,EGAPOLYNOMIALNOTETOEVALUATECARRYOVER,USETHERAWDATAEXPRESSEDINUNITSOFTHEROUTINEMETHODWITHOUTLOGARITHMICORANYOTHERTRANSFORMATIONIDF1612013E4ISOANDIDF2013ALLRIGHTSRESERVEDLICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013E1101001000100000100001000100CSCB1KEYCB1TOTALBACTERIALCOUNTOFBLANKSOLUTIONSANALYSEDIMMEDIATELYAFTERMILKSAMPLEINUNITS/MLCSTOTALBACTERIALCOUNTOFMILKSAMPLESINUNITS/MLRESULTSWITHINDIVIDUALSAMPLESETSTRENDLINECARRYOVER0NOTECARRYOVERINTHISEXAMPLEIS1FIGURE1EXAMPLECARRYOVEREFFECTWITHREGARDTOTOTALBACTERIALCOUNTINRAWMILK54STABILITYITISESSENTIALTOCHECKTHESTABILITYOFTHEINSTRUMENTWITHSUITABLESAMPLESFORMANYMICROBIOLOGICALMETHODS,REFERENCEMATERIALSARENOTAVAILABLEORTHEIRWIDESPREADAPPLICATIONUNDERFIELDCONDITIONSISNOTPOSSIBLEDUETOSHORTSHELFLIFEANDTHUSRESTRICTEDTRANSPORTABILITYCOMPENSATEFORTHISDEFICIENCYBYAREFERENCEMATERIALSUBSTITUTEORARINGTESTPROCEDURETHERELEVANTCHARACTERISTICSOFAREFERENCEMATERIALSUBSTITUTESHOULDBEASSIMILARASPOSSIBLETOTHENATUREOFTHECOMPONENTSANDTHEMATRIXINWHICHTHEMEASUREMENTTAKESPLACEWHENREFERENCEMATERIALSUBSTITUTESWITHLONGERSHELFLIFEAREAVAILABLE,THESTABILITYOFINSTRUMENTALMETHODSSHALLBECHECKEDTHROUGHOUTTHEWORKINGDAYANDALSODURINGTHEPERIODBETWEENINSTRUMENTSTANDARDIZATIONOPERATIONSQUALITYCONTROLINTHELABORATORYUSEACONTROLCHARTACCORDINGTOISO81962|IDF1282PROTOCOLSFORSTANDARDIZATIONANDSTABILITYCHECKSAREDESCRIBEDINISO81962|IDF128255PRECISION551GENERALFORGUIDANCEONTHEDETERMINATIONOFPRECISION,REPEATABILITYANDREPRODUCIBILITY,SEEISO57251,ISO57252,ISO81961|IDF1281,ANDISO161401IDF1612013EISOANDIDF2013ALLRIGHTSRESERVED5LICENSEDCOPYMRUNIVERSITITEKNOLOGIMALAYSIAUSER,UNIVERSITITEKNOLOGIMALAYSIA,27/06/20130701,UNCONTROLLEDCOPY,CTHEBRITISHSTANDARDSINSTITUTION2013BSISO162972013ISO162972013E552REPEATABILITYTHEREPEATABILITYCANBEESTIMATEDFROMALARGENUMBERN50100OFDUPLICATEMEASUREMENTSMADEONSAMPLESCOVERINGTHEWHOLEMEASURINGRANGEIFTHEREPEATABILITYISDEPENDENTONTHELEVEL,ITSHALLBESPECIFIEDASAFUNCTIONOFTHELEVEL,OTHERWISEANAVERAGEVALUECANBEUSEDFORTOTALBACTERIALCOUNTINRAWMILK,THEACCEPTABILITYLIMITSFORTHEREPEATABILITYSTANDARDDEVIATION,SR,AREAUNITSOF0,09LOG10UNITSFORCONTAMINATIONLEVELS2104CFU/MLBUNITSOF0,12LOG10UNITSFORCONTAMINATIONLEVELS2104CFU/ML553REPRODUCIBILITYESTIMATETHEREPRODUCIBILITYTHROUGHANINTERLABORATORYSTUDYACCORDINGTOISO57252FROMDUPLICATEMEASUREMENTSINREPRESENTATIVESAMPLESATTHELOWER,MEDIUM,ANDUPPERLEVELSINTHEMEASURINGRANGE,PREFERABLYOBTAINEDFROMATLEASTEIGHTCOLLABORATORSIFNORELATIONSHIPEXISTSBETWEENREPEATABILITYANDTHELEVEL,THISCANALSOBEASSUMEDTOBETRUEFORTHEREPRODUCIBILITYIFTHEREISARELATIONSHIPBETWEENTHEREPR
温馨提示
- 1. 本站所有资源如无特殊说明,都需要本地电脑安装OFFICE2007和PDF阅读器。图纸软件为CAD,CAXA,PROE,UG,SolidWorks等.压缩文件请下载最新的WinRAR软件解压。
- 2. 本站的文档不包含任何第三方提供的附件图纸等,如果需要附件,请联系上传者。文件的所有权益归上传用户所有。
- 3. 本站RAR压缩包中若带图纸,网页内容里面会有图纸预览,若没有图纸预览就没有图纸。
- 4. 未经权益所有人同意不得将文件中的内容挪作商业或盈利用途。
- 5. 人人文库网仅提供信息存储空间,仅对用户上传内容的表现方式做保护处理,对用户上传分享的文档内容本身不做任何修改或编辑,并不能对任何下载内容负责。
- 6. 下载文件中如有侵权或不适当内容,请与我们联系,我们立即纠正。
- 7. 本站不保证下载资源的准确性、安全性和完整性, 同时也不承担用户因使用这些下载资源对自己和他人造成任何形式的伤害或损失。
最新文档
- 游戏化教学在小学语文低年段识字中的应用与效果研究论文
- 花园及菜园管理制度
- 茶具洗消间管理制度
- 草莓收购点管理制度
- 苗木销售合同 (一)
- 财务会计工作计划 (七)
- 课程计划与课程标准
- 计算流体力学网格生成方法阅读笔记
- 湖北省孝感市安陆市2024-2025学年七年级下学期期中道德与法治试题(含答案)
- 自动控制理论课程设计课程教学大纲
- 某院检验科仪器设备档案
- 中铝中州矿业有限公司禹州市方山铝土矿矿山地质环境保护和土地复垦方案
- 职业卫生知识培训记录
- 起重设备维护保养记录(完整版)
- 网络信息安全培训课件-PPT
- 北京市医药卫生科技促进中心关于印发《首都医学科技创新成果转化优促计划实施方案(试行)的通知》
- (完整版)互联网+项目策划书
- THBLS 0011-2023 荆楚粮油 优质油菜籽生产技术规程
- 2023春国开社会调查研究与方法单元自测1-5试题及答案
- 美国AHA心肺复苏指南
- HAND-成本模块:移动平均成本-系统操作
评论
0/150
提交评论