大杯蕈液体培养及其生物学活性研究.pdf

河北大学 硕士学位论文 大杯蕈液体培养及其生物学活性研究 姓名：姚钗 申请学位级别：硕士 专业：微生物与生物药学 指导教师：王谦 20100601 ? i ? nfi ?clitocyle macima?v?u?d? ?n?*?!“/nfi ?;?e/d? “?n?4?nfi ? kh2po4 ?mgso47h2 o?enfi fl ?d?;? 3.00%? ? 0.10%?kh2po4 0.20%?mgso47h2o 0.10%* ?;?nfi ?w?d?w?0? ?yd ?fl ph !7“#$%?enfi fl ?d?12“dp7?abc7def$vgu? 3.08%h$?nfi ?);dpi?$v?j?k/);dplo 0.7dm ?);5$?n); 619 o?p); 667 om);?def?nms 4.63%h$nfi ?“t?bc?n* ? nfi fl ?d?“?a?b cdefgh?!“()? i=?jklmn?faopqr?fst?u vw?xy* z,#$?!“?_?abcdb?0_/?e?f ?geh/89* $ijk?lmno?2010p6q9n ? ?rs/tlmn?uvwxyuv?z?|?uwxg? ?uv?fqr?7?2?7?*?4? ?s?f?4?v?y?fa?w?* ? 1yw? ?pqnt?uv?* 2y=w? * ? ?4;?“” $ijk?lmno? 2010p6q9n ?jk?no? 2010p6q9n ? ?j?lmn?“?j?nfi fl ?%?!“? ?,?gz?$?n?b(jn?d9v? fl ?d?$j# j?;s* ?y?yd?fl 0?”?41? %?jy?7?w?7? ?7?ud?$v?j?y? ?y?y?y?“?u?77d$v“4*?p? ?j?y?#?y?y?%?y?%d? ?!“;7u/?*n?1?w?y?7?b( j? ?*?0? 3?13cm*?j 26?28? 23? 32?t?t?*?hw$ 60?65?o ?n? !j 80?90?/an?“#?$ ph !j 5.1?6.4* ? 1.1 ? fig1.1 clitocybe maxima nfi ?v?hu?%?z, ? ? 1 ? 3 ?u9m ?$v6* 1.2 ? n?d?submerged fermentation?fl ?yzn? ?;?:?f?d?af%bc?w=?deffg?u? ?0n$hi?;*n?fl ?yzd?j*yzd ?u?i?9vn?w?0?y)fi y% fl ? ji?ufl ?8* 70%?80%42627* 9vd?n?yyw?y?f:?n ? 1 ? 5 ?d?2uvw6“y?“28y“29y“?uq“ u“+?87?u?=*0w6“?u? ?“?80tyuq“uuv$?y?y?$un7?“i*w“?“?;j?v6?p:?m?!“? lm?hu=;!nfi ? ?0?);?lmnfi “t? ?bc7def$v?* ?w?;?n?=?731?s6?$v*?bu0? n?$vbc?+?=n?hu? ?3$v=+?u?t3f1?3)$v?3?a? ?yw?5b?-?i?;? 2.2.4.1 ?*?op ?24?yop?yfg?y?“h?yij?j?k“2.1.2.2”0? a(?wl=t?0.1mpa?121?u? 30min*f?%;m“2.2.2”0;? ? ?$?%?)ghm“2.2.3”0;? ? 3 ?* 2.2.4.2 ?*?op ?2v?y%nyo?yijy?e?j?k“2.1.2.2”0?ij? a(?=t?0.1mpa?121?u? 30min*f?%;m“2.2.2”0;? ? ?$?%?)ghm“2.2.3”0;? ? 3 ?* 2.2.4.3 ?*?op ?2v kh2po4ynaclycacl2ymgso47h2oykcl $j?q?3“2.1.2.2”? ?0?a(?=t?0.1mpa?121?u? 30min*f?%;m“2.2.2”0; ? ?$?%?)ghm“2.2.3”0;? ? 3 ?* 2.3 24.523.522.822.321.3 1.31.20.80.91.3 ( 2.2 ?)?*+%?9a tab2.2 multiple comparison of the effect of carbon sources on fermentation of clitocybe maxima ? 6789 :g/100ml; xi-21.3xi-22.3xi-22.8xi-23.5 ,-24.53.2*2.2*1.7*1.0* ./-23.52.2*1.2*0.7* 01-22.81.5*0.5* 2322.31.0* 45321.3 *p1.31.41.20.70.8 ( 2.4 ?)?*+%?9a tab2.4 multiple comparison of the effect of nitrogen sources on fermentation of clitocybe maxima ? 6789 :g/100ml; xi-22.4xi-22.7xi-22.9xi-24.5 de326.84.4*4.1*3.9*2.3* fgh24.52.1*1.8*1.6* ij22.90.5*0.2* klm22.70.3* ?n322.4 *p ?7?|y?:ij?“?8?*?e 2.3 7e 2.4 ? -?nfi 0?9v?o?:i$?1?jq?pn7?e?9vg?*?)?nfi d?$?j?) ? ?*+ 26.8g/100ml* 2.3.3 hijd?-.?ef 2.3.3.1 qr8+ =?q?nfi d?5n%?/0?)?e 2.5ye 2.6 7p 2.3 ( 2.5 “#$)?%*+ tab2.5 the effect of inorganic salt on fermentation of clitocybe maxima kh2po4mgso47h2ocacl2naclkcl 6789:g/100ml;28.928.727.126.726.4 1.20.70.61.10.9 ? 14 ( 2.6 “#$)?*+%?9a tab2.6 multiple comparison of the effect of inorganic salt sources on fermentation of clitocybe maxima “#$ 6789 :g/100ml; xi-26.4xi-26.7xi-27.1xi-28.7 kh2po428.92.5*2.2*1.8*0.2* mgso47h2o28.72.3*2.0*1.6* cacl227.10.7*0.4* nacl26.70.3* kcl26.4 *p tab2.8 data of orthogonal design and range analysis st _ abcd 6789 :g/100ml; 1111127.4 2122229.3 3133326.3 4212331.6 5223127.6 6231227.4 7313227.6 8321326.0 9332127.5 ab 127.66728.86726.93327.500 ab 228.86727.63329.46728.100 ab 327.03327.06727.16727.967 z1.8341.8002.5340.600 2.3.4.3 s9 ?e 2.8 ?4?nfi yzd? kh2po4(c)?(a) ?(b)mgso47h2o?d ?j a2b1c2d2?g?ws?e 2.9 ?m) ? 120h? ?* 32.2g/100ml?t? 9 ?0w 4 ?a2b1c2d3 ? )nfi fl ?d?j a2b1c2d2?|? 3.00%? 0.10%? kh2po40.20%?mgso47h2o 0.10%* ( 2.9 cdefghi%a tab2.9 comparison of the two culture mediums a2b1c2d2a2b1c2d3 6789:g/100ml;32.231.6 0.91.3 ? 3 ?jk?% 01.27.616.926.132.232.031.1 00.60.80.61.21.11.31.5 ? 3 ?jk?% 8.007.837.686.555.725.004.864.12 0.30.50.80.61.01.20.81.1 ? 3 ?jk?%?y? 4.1.3 11116.12 21226.34 31336.35 42126.28 52236.41 62316.32 73136.38 83216.42 93326.38 ab 16.2706.2606.287 ab 26.3376.3906.333 ab 36.3936.3506.380 z0.1230.1300.093 4.4.1.2 s9 ?)?-? ?nfi ?“6.486.42 0.250.34 4.4.2 ?-.?4?bc3? 4.4.2.1 qr8+ nfi ?12“ 11117.35 21227.48 31337.58 42127.39 52237.62 62317.45 73137.56 83217.69 93327.58 ab 17.4707.4337.497 ab 27.4877.5977.483 ab 37.6107.5377.587 z0.1400.1640.104 ? 4 ?|?n?-wfx%7.727.69 0.180.23 4.4.3 ?-.?4?dec? 4.4.3.1 qr8+ nfi ?x?2“ 11116.28 21227.10 31336.89 42127.11 52237.10 62317.02 73137.18 83217.23 93327.12 ab 16.9377.0377.023 ab 27.0777.1437.110 ab 37.1777.0107.057 z0.2400.1330.087 4.4.3.2 s9 ?)?-?nfi ?“7.337.23 0.210.25 4.5 0, ?%?“&;?412?nfi ?t*122?4 5j?12( 15min?6“ 3 ?1278 300w?“?|?t? 1/4m ?fg?3 3.5 ?t? ?w? ?h? 10h?5000r/min ”? 10minm?h?v?w? 1 ?y? /? 2 ?mv”?w?“?;? 4?w?dv* 5.1.2 ;k v?);?drosophila melanogaster ?lmn?!“c“* 5.1.3 ;k?% );?d? y?w ?f3$nfi ? 750ml m? 3k4 6.2g? ?k4“t?3? 62.5g ? fg“t?3(?ij? 82.5g ?v ?w( ? ?fgdo?m? 23min? 9 ? 250ml %bc0*121?u? 30min? ?3?e? 7g?x?u?v?+g0? ?wy+* 5.1.4 klm?n?ef ?ch/hnfi ? 0.00%y1.54%y3.08%y4.63%7 6.18%u$ 5 ?2 _);?);* ac 3 ?,- 8h ?j);?!?np*a?f 5 );? 25?*(?c?r?*ghyy(;?(* ? 5 ?-?g?k!?%klm?!5?ef ?ch/hnfi ? 0.00%y1.54%y3.08%y4.63%7 6.18%u$ 5 ?2 _);?);* ac 3 ?,- 8h ?j);?!?np*a?f 5 );? 25?*(?c?r?*agh?wo(;? 6d ? ?);5$?%np* 5.2.3 ?-.?d;kop?ef ?ch/hnfi ? 0.00%y1.54%y3.08%y4.63%7 6.18%u$ 5 ?2 _);?);* ac 3 ?,- 8h ?j);?!?np*ac 3 ? ,- 8h ?j);?!?a? 50 o?a 7d ?* lm);|dpyp?lm?0?*p?3 qfi * ?s? 909113?a ? 6.1.3 qr ?fl ?(?t 20cm*?p 6.1 c 6.1 fig6.1 labyrinth 6.1.4 stuvw ?y/)?ij? 27%?ij 19%?nj 16%? 16%?f? 13%? $? 3%?e? 2.3%? 0.1%? 0.5%? ?1$8? 0.1%* ?q?j 5 ?a 10 o?2? 0.00%y1.54%y3.08%y4.63% y 6.18%;!nfi ? 3 l?fi ?* ? 24h?o?$w*a? 10 ?a 3 ?* ?”v 35min*y 4 l?4? .?x?p?ws?;?e* a? 5 ?|da?q?j 5 ?a 8 o? 0.00%y1.54%y3.08%y4.63% y6.18% ;!nfi ? 3 l?fi ?* a?a? 20min?y 4 l?lm?w?0=? ?jx?4?w0 7s =?jly?(?4?($j ef!?5* 6.3 !?nfi ? ?bc?d* +?jq?1w|*0 d ?“t!n?bc?6zu$?v?;!j 4.63%* 6.3.2 ?-.?z|? 6.3.2.1 qr8+ nfi ?def?e 6.1 ?h* ( 6.1 ?-)l?%*+ tab6.1 the effect of polysaccharide of clitocybe maxima on anti-weary of white mouse ?-?:%;l?wi:;qr:s; a08356.5215.86 b1.548398.67*27.58 c3.088428.55*16.78 d4.638427.57*35.86 e6.1884