骨髓间充质干细胞移植抑制肺气肿炎症反应及细胞凋亡.doc

骨髓间充质干细胞移植抑制肺气肿炎症反应及细胞凋亡赵晓建，卢彩平，褚伟伟，甄强，谭国梁，张亚肖，王仁锋，张冰，刘家宝河北省石家庄市第一医院胸外科，河北省石家庄市050000Bone marrow mesenchymal stem cell transplantation suppresses emphysema-induced inflammation and apoptosisZhao Xiao-jian, Lu Cai-ping, Chu Wei-wei, Zhen Qiang, Tan Guo-liang, Zhang Ya-xiao, Wang Ren-feng, Zhang Bing, Liu Jia-baoDepartment of Thoracic Surgery, First Hospital of Shijiazhuang City, Shijiazhuang 050000, Hebei Province, China背景：骨髓间充质干细胞移植可以通过旁分泌机制改变周围区微环境，通过促进血管新生、抑制细胞凋亡和维护自主神经功能稳定等作用，可用于治疗肺功能严重损害。目的：通过骨髓间充质干细胞移植，观察其对实验性肺气肿炎症反应及细胞凋亡的抑制作用。方法：24只Wistar雌性大鼠随机分健康对照组、模型组及实验组，后两组烟熏加气管内滴注猪胰弹性蛋白酶法建立肺气肿模型。建模后大鼠尾静脉注射骨髓间充质干细胞，观察细胞移植后大鼠肺组织的病理学变化、检测支气管肺泡灌洗液中细胞数及肿瘤坏死因子水平、肺泡壁细胞凋亡指数。结果与结论：模型组、实验组大鼠的肺组织都存在不同程度的肺气肿病理改变，实验组较轻，差异有显著性意义(P 0.01)；实验组肿瘤坏死因子水平及肺组织细胞凋亡指数均低于模型组，差异有显著性意义(P 0.01)。提示骨髓间充质干细胞通过抑制实验性肺气肿炎症反应及细胞凋亡，改善了肺气肿病理学，对肺气肿有明显的治疗作用。中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程全文链接：关键词：干细胞；骨髓干细胞；细胞移植；肺气肿；烟熏；弹性蛋白酶；模型；炎症反应；细胞凋亡；免疫组化； Abstract：BACKGROUND:Bone marrow mesenchymal stem cells transplantation can change the surrounding microenvironment through paracrine mechanisms, and can be employed for treatment of serious damage to lung function through the promotion of angiogenesis, inhibition of apoptosis and maintaining functional stability of autonomic nervous system.OBJECTIVE:To observe the inflammatory reaction in experimental emphysema and inhibition of apoptosis through bone marrow mesenchymal stem cells transplantation.METHODS:Twenty-four Wistar female rats were randomly divided into three groups: healthy control group, model group and experimental group. In the latter two groups, smoking and endotracheal instillation of porcine pancreatic elastase were performed to establish emphysema models. After modeling, bone marrow mesenchymal stem cells were injectedviatail vein in the experimental group. Pathological changes of the lung, the level of tumor necrosis factor-alpha and cell number in the bronchoalveolar lavage fluid as well as apoptotic index in lveolar walls were detected after cell transplantation.RESULTS AND CONCLUSION:In the model and experimental groups, pathological changes of lung tissues were observed to different extent. The lung pathological changes were slighter in the experimental group than the model group (P 0.01). The level of tumor necrosis factor-alpha and apoptotic index in lung tissue were lower in the experimental group than the model group (P 0.01). These findings indicate that bone marrow mesenchymal stem cells can improve emphysema pathologically through inhibition of inflammatory response and apoptosis in experimental emphysema.中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程全文链接：Key words：stem cells;mesenchymal stem cells;bone marrow;cell transplantation;pulmonary emphysema;bronchoalveolar lavage fluid;中图分类号:R394.2基金资助:石家庄市科技支撑计划项目(121461793)通讯作者:卢彩平，硕士，主治医师，河北省石家庄市第一医院内分泌科，河北省石家庄市 050000作者简介: 赵晓建，男，1981年生，河北省石家庄市人，汉族，2007年山西医科大学毕业，硕士，主治医师，主要从事普胸科疾病研究。引用本文:赵晓建,卢彩平,褚伟伟等. 骨髓间充质干细胞移植抑制肺气肿炎症反应及细胞凋亡J.中国组织工程研究, 2014, 18(6): 906-911.Zhao Xiao-jian,Lu Cai-ping,Chu Wei-wei et al. Bone marrow me