NM23-H1与MUC1基因在甲状腺癌中的表达及其意义

null nullNM23nullH1 MUC1 y J F V r # i l刘志勇null 欧阳忠 K 1 ! null null ) M y H1( NM 23nullH1) * 1( MUC1) y J F V r # 5 i l bZ E null RTnullPCR m s / _ 60 J F NM23nullH1 MUC1 y V r b T null MUC1 y J F V r q 75null0%( 45/60) ；MUC1 % M F _ q 70null6% ( 24/34), % M F _ q 80null 8%( 21/26) (P 0null05)bNM23nullH1 y J F V r q 63null 3% (38/60)； NM23nullH1 % M F _ q 73null 5%( 25/34), % M F _ q 50null0% (13/ 26) ( P 0null05) .T he positive rate of NM23nullH1 in 60 specimens of thyroid carcinoma was 63null 3%( 38/60) ,which was 73null5%(25/ 34) in those without metastasis and 50null 0% (13/ 26) in those with metastasis( P 1 V r , 0 , = 0 | b1 T T b ad 9 ) 5 Z _ d 9 ) , P 0null05)bNM23nullH1 y J F % M 1 (P 0null05)b(V 1)V 1null MU C1 NM 23nullH1 y V r h 1 ( )s MUC1 y V r MUC1 y V r| NM23nullH1 y NM23nullH1 y| % Mnull null 21245101325139h null h null r null s null 20126784121912349445 aNM23nullH1 MUC1 V r J F 1 22 _ NM23nullH1 V r V r S 20 _ MUC1 V r , 6 MUC1 Vr S NM23nullH1 V r ,S NM23nullH1 V r MUC1 V r W i A M 1 (r= 0null8869)b) NM23 y - K M y , h 3 i a 3 i M 1 5bMUCl y B E s 1 J F M 1 F y , MUC1 y 4 V J F % Vr # V r T , 7 J F % V r b B V , MUCl y I E s 1 J F S 6 8bWeiss 9 RTnullPCR_ J F h # J F h M F MUC1 V r , T V , RTnullPCR _ MUC1V r V T J F - % , O s 03 G bMUC1 y I MUC1 J F % # V r , s C , y N F V A C ； MUC1 c V M _ (VNTR) , 1 f T , MUC1 c F 3 y 0 s 8 2(GRB2) SH2M T , | d (ras), V % 0 b B V , MUC1V r V nullnull (betanullcatenin)Enull T (Enullcadherin)% * s 0 , M 10, 11 b L T A U , 60 J F MUC1 y _ q 75null0%, % M F _ q 80null8%, % M F _ q 70null6%, % M # h A 1 b9null1785null D 0 2010M 8 36 15 null Jiangsu M ed J, August 2010, Vol 36, No. 15 , NM23nullH1 MUC1 y Y V I h s V | s ,F J F % 0 , i % * s 0 , M % M b M y NM23 Steey 1988M V M. ? s b M% V r M % 10 b ? , NM23 y V r ? M M 1 , nm23 y V r M ? 3 T 12bNM23 NM23nullH1, nm23nullH2 , L nm23nullHl ? M ? b L T A U , NM23nullH1 J F % M _ q 50null0%, % M _ q 73null5%,H J F h _ q 67null9%, NM23nullH1 J F % M 1 bNM23I = ( NDPK) , &9 NDPK , NDPK 5 1 8 ,i G | . 1 bNDPK Y % a a # s a； 8 ； NDPK h ? , a； 8 p , d 8 % ? Z , % s 13, 14b L T A U , 22 _ NM23nullH1 V r V r S 20 _ MUC1 V r , 6 MUC1 V r S NM23nullH1 V r ,NM23nullH1 MUC1 B M 1 b N 4 U , J F F i NM23nullH1 MUC1 V r C , V ? J F % M bNM23nullH1 MUC1V r , ? 0 V ? ,_ ) ? 3 M q V ? v , V ? b9 , _ J F F NM23nullH1 MUC1 V r f J F 3 N , V S J F 0 S ,i 5 , 7 ? F ? 0 s y y 4 L b I D 1 null Agou F, Ravch S, kopper L, et al. Singlestrand DNA specifityanalysis of human nucleoside diphosphate kinase B J . J Biolchem, 1999, 274( 28) : 19630null19638. 2 null Lee E, Jeong J, Kim SE, et al. Multiple functions of NM23nullH1are regulated by oxidonullreduction system J. Plos One, 2009,4( 11) : 7949. 3 null OkabenullKado J, Kasukabe T , Honma Y, et al. ExtracellularNM23 protein promotes the growth and survival of primarycultured human acute myelogenous leukemia cells J . CancerSci, 2009, 100( 10) : 1885null1894. 4 null Kawakubo Y, Sato Y. Expression of nm23 protein in pulmonullnary adenocarinoma: inverse correlation tumor progression J . Lung Cancer, 1997, 17( 1) : 103null113. 5 null ,R , null COXnull2 # nm23nullH1 V r # 5 i l J . D 0 , 2007,3( 10) : 1002null1003. 6 null Yuan S, Shi C, Lv Y, et al. A novel Bacillus CalmettenullGuerinnullbased breast cancer vaccine that coexpresses multiple tandemrepeats of MUC1 and CD80 breaks the immune tolerance andinhibits M UC1nullpositive breast cancer growth J . CancerBiother Radiopharm, 2009, 24( 5) : 607null613. 7 null Domschke C, Schuetz F, Ge Y, et al. Intratumoral cytokinesand tumor cell biology determine spontaneous breast cancernullspecific immune responses and their correlation to prognosis J . Cancer Res, 2009, 69( 21) : 8420null8428. 8 null Reddish M, Maclean GD, Koganty RR, et al . AntinullMU C1class I restricted CTLs in metastatic breast cancer patientsimmunized with a synthetic MUC1 peptide J . Int j cancer,1998, 76( 6) : 817null823. 9 null Weiss M, Baruch A, Reydar I, et al. Preoperative diagnosisof thyroid papillary carcinoma by reverse transcriptase polynullmerase chain reaction of the MUC1 gene J . Int J Cancer,1996, 66( 5) : 55null59. 10 null Charpin C, Garcia S, Bonnier P, et al. Prognostic significanceof nm23/ NDPK expression in breast carcinoma, assessed on10nullyear follownullup by antomated and quantitative immunocytonullchemical assays J . J Pathol, 1998, 184( 4) : 401null407. 11 null Fujita K, Denda K, Yamamoto M, et al. Exprossion of MU C1mucins inversely correlated with postnullsurgical survival ofrenal cell carcinoma patients J . Br J Cancer, 1999, 80( 1) :301null308. 12 null Steg PS, Cohn KH. Tumor metastasis and nm23: current connullcepts J . Cancer, 1991, 3(7) : 257null262. 13 null Arai T , Yanshita T , Urano T, et al. Preferential reduction ofNM23nullH1gene product in metastatictissue from papillary andfollicular carc