磁珠在临床以及生物科学与技术中的运用.ppt

magneticnano andmicroparticlesinbioscienceandbiotechnology ivosafarikandmirkasafarikovaceskebudejovice czechrepublic typeofmagneticnano andmicroparticles multidomain singledomainorsuperparamagneticmagnetite fe3o4 ferrites meo fe2o3 me ni co mg zn mn maghemite fe2o3 greigite fe3s4 iron nickel magneticnano andmicroparticles inorganicmaterial but presentinbiologicalsystemsusableforavarietyofbio applications importantdates 1962 lowenstam biochemically precipitatedmagnetiteasacappingmaterialintheradula tongueplate teethofchitons marinemollusksoftheclasspolyplacophora 1975 blakemore magnetotacticbacteria magneticmaterialinteethofchitons hardeningthetoothcaps enablingthechitonstoextractandeatendolithicalgaefromwithintheouterfewmillimetersofrocksubstrates magnetotacticbacteria variousmorphologicaltypesexistgram negativeprokaryotesmagneticnanoparticlespresentinmagnetosomesfoundinsedimentsindiverseaquaticenvironments magnetotacticbacteria providedbydr d sch ler germany magnetosomes composedfrommagnetite fe3o4 orgreigite fe3s4 ca50nmindiametercoveredwithalipidicmembraneimportantfornavigationofthebacteriaintooxic anoxiclayerbiocompatiblecharacter phospholipidbilayer possibleapplicationsinbiosciences magnetosomes providedbydr d sch ler germany magnetosomes providedbydr d sch ler germany biogenicmagneticnanoparticles magnetotacticbacteriamagnetotacticalgaeprotozoainsectfishamphibiansreptilesbirdsmammals animalmagnetite magnetosomechainsfromthefrontaltissuesofchinooksalmons mannetal j exp biol 140 1988 35 49 importanceofanimalmagnetite mostprobablyinvolvedinmagnetoreception whatisthecurrentposition whichdirectiontomove interactionofmagnetitenanoparticles chainsofmagneticnanoparticles withthesensingcells non physiologicalpresenceofmagneticnanoparticles foundinhumanbrainincaseofneurodegenerativediseasesdisruptionofnormalironhomeostasisinthebrainearlydetectionofneurodegenerativediseasesusingmri magneticnanoparticlesinmeteoritesfrommars meteoritemagneticnanoparticlesresemblemagnetosomesfromearthmagnetotacticbacteriahypothesis wasthereatransferoflivingsystemsfrommarstoearth let sreturnbacktoearth magneticnano andmicrostructuredmaterialsforbioapplications usefulformanyapplicationsinbiosciences biotechnologiesandenvironmentaltechnologiesenabletolabeldiamagneticstructuresenableseparationordetectionofmagneticallylabeledstructuresbothseparationandnon separationapplicationsareimportant whichnano andmicrostructuredmagneticmaterials threeimportantbio biocompatible inthebroadestsenseofthisword biodegradablebiomimetic biocompatiblematerials biocompatibilityinthebroadestsenseofthewordminimaltoxicitystabilityofnanoparticlesinbodyfluidspossibilityforimmobilizationofbiologicallyactivecompounds cellorganelles cells activatednanoparticles reproducibleinteractionwithbiosystemsenablelabelingofbiostructures formagnetoassays biopolymerbasedparticles pegylatedstructures magnetoliposomes biopolymer basedffs self assembledmagneticstructures biodegradablematerials importantfromthepointofviewofpossibleclinicalapplications e g drugtargeting nanoparticlesformedbybiodegradable bio polymermagnetoliposomesandrelatedstructuresstarch basedmagneticfluids biomimeticmaterials analoguesofbacterialmagnetosomesferritinxmagnetoferritin importantdata 1960s pappell firstferrofluids 1000hoursofmagnetitemilling 1966 magneticallycontrolledthrombosisofintracranialaneurysms1970s increaseofinterestinbiomagneticresearchandtechnology1980 massart chemicalsynthesisofaqueousferrofluidwithoutdetergents 1980s commerciallyavailablemagneticparticles magneticfluids examplesofmagneticnanostructuredmaterials ferrofluidcanbeinfluencedbythepresenceofexternalmagneticfield syntheticbiocompatiblemagneticnanoparticlesandcomplexescontainingthem biocompatiblemagneticfluidsmagnetoliposomesmagneticnano andmicroparticlesmagnetoferritin magneticmicroparticles dynabeadshomogeneoussizedistributionsimplerecoverywithsmallpermanentmagnetsmagneticnanoparticlespresentinsidethebeadsavailableinplain activatedandbioactiveform examplesofofmagneticnano andmicroparticlesapplications frommolecularbiologytoenvironmentaltechnologiesmanipulationofmicrolitersaswellasmillionoflitersmanipulationinsuspensionsystemsbothseparationandnon separationtechniquesareimportant immobilizationandmodificationofbiologicallyactivecompounds possibilityofsimpleremovaloftheimmobilizedmoleculesorcellsfromthesystemimmobilizationofenzymes antibodies lectins oligonucleotides affinityligandsmodificationofproteinsviamagneticpegimmobilizationinmagnetoliposomesmagnetosomesfromgeneticallyengineeredmagnetotacticbacteriacarryingtargetproteins isolationofbiologicallyactivecompounds directseparationoftargetsfromcrudesamplescontainingsolidsaffinitymagneticbeadsormagnetictwo phasesystemspreconcentrationoftargetmoleculesfromlargevolumesofcrudematerialspolya mrna dna oligonucleotidesenzymeslectinsdna rnabindingproteinsantibodies antigensxenobiotics bothorganicandinorganic highgradientmagneticseparators testtubemagneticseparators automatedsystems kingfishermlthermo usa kingfisher96thermo usa te magstecan japan determinationanddetectionofbiologicallyactivecompoundsandxenobiotics immunomagneticassays magneticparticlesusedforseparationoftargetanalyte magnetoimmunoassays magneticparticlesserveasdetectablelabels magneticsubstratesmagneticsolid phaseextractionnewprinciplesforthedetectionandquantitationofbothmagneticlabelsandbiologicalstructurestested giantmagnetoresistance magnetoelasticity magneticatomicforcemicroscopy automatedsystemsforimmunoassays combinationofspecificmagneticseparationandsensitivedetection enzyme radioactive chemiluminescentorfluorescent differentassayformatscanbeused sandwichxcompetitiveimmunoassays genericsolidphasescanbeused streptavidin secondaryantibody proteina beckmancoulter saccessimmunoassaysystem magnetoimmunoassays magneticnanoparticlesserveasalabelpossibilitytoreplaceenzymes radioisotopes magneticpermeabilityreader euris sweden top magneticassayreader quantumdesign usa bottom magneticsolid phaseextraction detectionofcrystalvioletinwatermagnetitewithimmobilizedphthalocyaninedyeusedasaselectiveadsorbentcurveb 0 5 gofdyein1000mlofwatersensitivity0 5ppbachieved safarik i safarikova m detectionoflowconcentrationsofmalachitegreenandcrystalvioletinwater waterres 36 2002 196 cells immunomagneticseparationoftargetcellsandcellorganellesdetectionandseparationofcancercellsisolationofstemcellsseparationofmicrobialpathogensstudyofcellfunctiontransfectionofcellsmagnetictwistingcytometrymagneticlabelingofcellsmritrackingoftransplantedcells magnet modified labelingoft lymphocyteswithmagneticnano andmicroparticles e colio157boundtodynabeads principleofims large scaleimmunomagneticseparationofcells severalmagneticseparationdevicescommerciallyavailableclinimacsemploysmagneticnanoparticlesforcellslabelingandhgmssystemforseparation magnetotacticbacteria biotechnologyproductionofnativeorgeneticallyengineeredmagnetosomesnon destructivedomainanalysisradionucliderecovery drugandradionuclidetargeting accumulationofactivecompoundsinthetargetorganortumorferrofluids magnetoliposomes ferrofluidmodifiederythrocytes magneticpolymercomposites magneticdrugtargeting mtc compositeofelementalironandactivatedcarbonwithanticancerdrugadsorbedontoit ferxinc sandiego ca usa magneticfield bodysurface magneticfluidhyperthermia cancertreatmentheatingoftissueslabeledwithmagneticparticlesto42 46 cafterexposuretoalternatingmagneticfieldmagneticfluids magnetoliposomes magneticsingle ormulti domainparticlescombinationwithdrugtargeting sketchofthefirstprototypemfhtherapysystem mfhhyperthermiesystemegmbh berlin germany prof jordan contrastincreasingduringmri superparamagneticcontrastagentsbiocompatibleferrofluids magnetoliposomesantibody conjugatedmagnetitenanoparticles mriofbonemarrow pegylatedmagnetoliposomesasabonemarrow seekingmrcontrastagent potentialmedicalapplications treatmentofaidsendocytosisofferrofluidsbycancercellsbraintumorsensingartificialsphinctermusclemagneticpolymers artificialmuscle environmentalchemistryandtechnology wastewaterandpotablewaterpurificationusingmagnetitemicroparticles siroflocprocess australia magneticallymodifiedmicrobialcellsforxenobioticsremovalmagneticsolid phaseextractionforthepreconcentrationofthetargetanalytesmagneticallymodifiedenzymesforthedetectionoftheirinhibitors heavymetals pesticidesetc schemeofsiroflocprocess inhibitionofmagneticallymodifiedtrypsin dependenceoftheinhibitionofthetrypsinactivityontheconcentrationofag andpb2 dependenceoftheinhibitionofthetrypsinactivityontheconcentrationofthionin 4 aminobenzamidin safranin andbacitracin adsorptionofmagneticnanoparticlesonyeastcells interactionofsaccharomycescerevisiaecells bakersyeast brewersyeast withwater basedferrofluidsmagneticallymodifiedyeastcellscanbeusedasanadsorbentfortheseparationofbiologicallyactivecompoundsandxenobiotics bothorganicandinorganic adsorptionoforganicdyesispresented futuretr