HPLC法测定复方灵贞胶囊中齐墩果酸的含量134957.doc

hplc:, -(88120.040.02)1 ml/min220 nm 38.88972.0 g/ml(r=0.9992),100.81%,rsd=0.54% , abstractobjective to establish a method for determination the content of oleanolic acid in compound lingzhen capsules by hplc. methods c18 column (lichrospher 6.0 mm150 mm, 5 m) was applied with ch3oh-h2o-ch3cooh-(c2h5)3n (88120.040.02) as its moble phase (flow rate1 ml/min). detection wavelength was 220 nm. results the linearity of oleanolic acid was in the range of 38.88972.0 g/ml, r=0.999 2. average recovery of oleanolic acid was 100.81% with rsd=0.54%. conclusion this method was simple and sensitive, the results were accurate and quick. it is available for the quality control of compound lingzhen capsules.key wordscompound lingzhen capsulesoleanolic acidhplccontent determination(ganoderma)(fructus ligustri lucidi),20051hplc2-6,hplc,1 lc-10at,spd-10a,n2000,(),110709-200304,2003050120030502200305032 2.1 lichrospher c18(6.0 mm150 mm,5 m)-(88120.040.02)1 ml/min220 nm20 l1.512.2 60 4 h,1 ml200 g,2.3 10 g,150 mg,10 ml,8 ml,20 min,2.4 ,2.32.5 20 l,2.2,20 l, -(88120.040.02)1 ml/min220 nm 38.88972.0 g/ml(r=0.9992),100.81%,rsd=0.54% , determination of oleanolic acid in compound lingzhen capsules by hplcqian yi-fan, yin hui-jing, di bin, et a1.nanjing zhongke biochemical technology co.ltd, nanjing 210008, china2.yanbian university, yanji 133000, china3.china pharmaceutical university, nanjing 210009, chinaabstractobjective to establish a method for determination the content of oleanolic acid in compound lingzhen capsules by hplc. methods c18 column (lichrospher 6.0 mm150 mm, 5 m) was applied with ch3oh-h2o-ch3cooh-(c2h5)3n (88120.040.02) as its moble phase (flow rate1 ml/min). detection wavelength was 220 nm. results the linearity of oleanolic acid was in the range of 38.88972.0 g/ml, r=0.999 2. average recovery of oleanolic acid was 100.81% with rsd=0.54%. conclusion this method was simple and sensitive, the results were accurate and quick. it is available for the quality control of compound lingzhen capsules.key wordscompound lingzhen capsulesoleanolic acidhplccontent determination(ganoderma)(fructus ligustri lucidi),20051hplc2-6,hplc,1 lc-10at,spd-10a,n2000,(),110709-200304,2003050120030502200305032 2.1 lichrospher c18(6.0 mm150 mm,5 m)-(88120.040.02)1 ml/min220 nm20 l1.512.2 60 4 h,1 ml200 g,2.3 10 g,150 mg,10 ml,8 ml,20 min,2.4 ,2.32.5 20 l,2.2,20 l,2.6 ,1 ml0.972 mg,0.41.02.05.0 ml10 ml,38.8897.2194.4486 g/ml420 l,(x)(y),y=3 814.9xc30 769, r=0.999 238.88972.0 g/ml2.7 2.7.1 ,2.3,1 d6,3.64 mg/,rsd=2.0%2.7.2 ,2.3,3.59%6.75%,rsd=1.9%2.8 194.4 g/ml,6,rsd=1.7%2.9 ,012468 h,rsd=1.7%8 h2.10 ,1 ml509.6 g1.22.02.8 ml,75 mg,3,3100.2%1.0%100.9%1.2%101.3%1.7%1 1 2.11 20 l,22 3 ,hplc,hplc,hplc