摘要题目 (宋体, 三号字, 加粗, 居左).doc

摘要题目 (宋体, 三号字, 加粗, 居左)作者1 , 通讯作者2* (宋体, 小四号字,加粗，通讯作者名字右上方带星号)1部门,单位,地址,城市,国家 (宋体, 五号字)2部门,单位,地址,城市,国家Gallic acid metabolites represent a unique family of plant secondary metabolites present widely in the plant kingdom in the forms of polygalloyl esters of polyols, galloyl conjugates of catechins1,2. They have many bioac-tivities such as anti-inflammatory3, antimutegenesis 4,5, antiviral activities6. 宋体, 小四号字, 单倍行距参考文献应用方括号和数字标注1 and 2,3 中文英文皆可关键词: Gallic acid metabolites(宋体, 小四号字)致谢: This work was supported by (宋体, 小四号字, 单倍行距)参考文献:1姓名, 姓名 (年)文件名.杂志名, 卷期, 页码范围. (宋体， 小五号字, 单倍行距)2A.B. Smith, C.D. Brown (Year)File name. Journal, Vol, Initial page. *通讯作者E-mail：Structure-Activity Relationships in the Bioactivities of Gallic Acid Metabolites and Their Interactions with BioplymersHuiru Tang1*, Chaoni Xiao1 and Baolu Zhao21State Key Laboratory of Magnetic Resonance and Molecular and Atomic Physics, Wuhan Institute of Physics and Mathematics，The Chinese Academy of Sciences，Wuhan 4300712State Key Laboratory of Brain and Recognition Laboratory, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, ChinaGallic acid metabolites represent a unique family of plant secondary metabolites present widely in the plant kingdom in the forms of polygalloyl esters of polyols, galloyl conjugates of catechins1,2. They have many bioactivities such as anti-inflammatory3, antimutegenesis 4,5, antiviral activities6. These metabolites readily bind with collagen and cellulose with hydrophobic interactions8, and possess outstanding antioxidant and neuroprotective acitivites9. Here, we will report the structure-activity relationships in the neuroprotective activities and the interactions with biopolymers for the GA metabolites. We will further discuss some most recent results from our metabonomic investigation on the effects of gallic acid on the endogenous metabolism of rodent models. Keywords: Gallic acid metabolitesAcknowledgements: Financial supports from NSFC and the Chinese Academy of Sciences (20575074, 06S138) are acknowledged.Rererences:1V.L.Singleton (1981), Adv.Food Res. 27:149.2 N.Niho, M.Shibutani et al (2001), Food Chem.Toxicol. 39:1063.3 B.H.Kroes, A.J.J.Vandenberg et al (1992), Plant.Med. 58:499.4 S.C.Chen, K.T.Chung (2000), Food Chem.Toxicol. 38:1.5 M.Kawada, Y.Ohno et al (2001), Anti-Cancer Drugs, 12:847.6 H.X.Xu, S.Kadota et al (1994), Heterocycles 38:167.7 H.R.Tang, A.D.Covington, R.A.Hancock (2003), Biopolymers, 70:403.8 H.I.Oh, J.E.Hoff, G.S.Armstrong & L.A.Haff (1980), J.Agri.Food Chem. 28:394.9 Z.B.Lu, G.J.Nie, P.S.Belton, H.R.Tang & B.L.Zhao(2006), Neurochem.Int., 48:263. *E-mail：Huiru.tang 构建“转录因子-启动子”耦联载体开展植物生物碱次生代谢工程张磊1，陈万生2*1第二军医大学长征医院药学部，上海市凤阳路415号2000032第二军医大学药学院生药学教研室，上海市国和路325号200433萜类吲哚生物碱(Terpenoid Indol Alkaloid, TIAs)是一大类有着重要药用价值的植物生物碱。夹竹桃科长春花（Catharanthus roseus）可以合成多达百余种TIAs，其中许多被广泛地作为药物应用。植物TIAs生物合成途径的研究已经相当深入。一种受茉莉酸甲酯诱导的植物转录调控因子ORCA3，通过调控初级和次级代谢途径中的关键酶基因的表达来调节TIAs合成。通过构建传统的35S启动子植物表达载体，分别对转录因子ORCA3和关键酶基因牻牛儿醇羟化酶(geraniol 10-hydroxylase, g10h)进行了单转和双基因共转化。结果显示在长春花中同时过量表达orca3和g10h不能取得最佳的含量提升效果。进一步通过基因融合(Gene fuse)技术，用带有甲基茉莉酸诱导响应元件（Jasmonate- and elicitor-responsive element, JERE）特异序列的异胡豆苷合成酶（strictosidine synthase, STR）启动子替换g10h野生型启动子，和ORCA3一起构成耦联植物表达载体转化长春花。实时定量PCR结果显示通过对g10h启动子的替换，ORCA3可以通过作用于JERE调控g10h的表达。转化发根中目标产物长春碱的含量为1.32 mg/g，是对照（0.33 mg/g）的4倍。阿玛碱的含量为0.87 mg/g，是对照（0.23 mg/g）的3.8倍。但是长春质碱的含量（0.19