NEB内切酶产品具体Buffer活性表.doc

NEBuffer Activity Chart for Restriction EnzymesNew England Biolabs provides a color-coded 10X NEBuffer with every restriction endonuclease to ensure 100% activity. To help select the best conditions for double digests, this chart shows the optimal (supplied) NEBuffer and approximate activity in the four standard NEBuffers for each enzyme. If BSA is supplied with an enzyme, it is included in all NEBuffer activity reactions. In addition, the table also shows recommended reaction temperature, heat inactivation temperature and recommended diluent buffer. Please click here for additional information about: Unique NEBuffer Activity| Double Digestion | Heat Inactivation | Activity at 37C | Diluent Buffers | Time-Saver Enzymes | High Fidelity (HF) Restriction Enzymes This chart has been updated to reflect the change of 54 restriction enzymes to NEBuffer 4 on Nov.1, 2008.Click for the Chart Legend, Icon Description A | B | C | D | E | F | H | I | K | M | N | P | R | S | T | X | Z | EnzymeSupplied NEBuffer% Activity in NEBuffersHeatInactiva-tion CIncuba.Temp.CDiluent1234AatIINEBuffer 4050501006537AAcc65INEBuffer 3 + BSA1075100256537AAccINEBuffer 45050101008037AAciINEBuffer 32550100506537AAclINEBuffer 4 + BSA10100100No37BAcuINEBuffer 4 + SAM50100501006537BAfeINEBuffer 42550251006537AAflIINEBuffer 4 + BSA50100251006537AAflIIINEBuffer 3 + BSA2575100508037BAgeINEBuffer 11005010756537CAhdINEBuffer 4 + BSA257501006537AAleINEBuffer 41025101006537AAluINEBuffer 4100100751006537BAlwINEBuffer 450100101006537AAlwNINEBuffer 450100501006537AApaINEBuffer 4 + BSA255001006525AApaLINEBuffer 4 + BSA10010010100No37AApeKINEBuffer 3257510050No75BApoINEBuffer 3 + BSA1075100758050AAscINEBuffer 4010101006537AAseINEBuffer 3NR75100 ddNR6537BAsiSINEBuffer 4 + BSA501001001008037BAvaINEBuffer 41075101008037AAvaIINEBuffer 45075101006537AAvrIINEBuffer 4100100501008037BEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentBaeGINEBuffer 11007510506537ABaeINEBuffer 4 + BSA + SAM50100501006525ABamHINEBuffer 3 + BSA75100100 dd100No37ABamHI-HFNEBuffer 41005010100No37ABanINEBuffer 4 + BSA50100501006537ABanIINEBuffer 410010501008037ABbsINEBuffer 210010025756537BBbvCINEBuffer 450100101008037ABbvINEBuffer 210010025756537BBccINEBuffer 1 + BSA1005010506537ABceAINEBuffer 3 + BSA1001001001006537ABcgINEBuffer 3 + SAMNRNR100 ddNR6537ABciVINEBuffer 41005001006537CBclINEBuffer 350100100 dd75No50ABfaINEBuffer 47550101008037BBfuAINEBuffer 3075100106550BBfuCINEBuffer 4 + BSA10050251008037BBglINEBuffer 35075100506537BBglIINEBuffer 3107510010No37ABlpINEBuffer 45010010100No37ABmgBINEBuffer 3 + BSA025100106537CBmrINEBuffer 275100751006537BBmtINEBuffer 22510025506537BBpmINEBuffer 3 + BSA751001001006537BBpu10INEBuffer 31025100258037BBpuEINEBuffer 4 + SAM50100101006537BBsaAINEBuffer 4100100100100No37CBsaBINEBuffer 450100751008060BBsaHINEBuffer 4 + BSA501001001008037ABsaINEBuffer 475751001006550BBsaJINEBuffer 41001001001008060ABsaWINEBuffer 4 + BSA50100501008060ABsaXINEBuffer 47510010100No37BBseRINEBuffer 4100100751006537ABseYINEBuffer 31050100506537BBsgINEBuffer 4 + SAM5075501006537BBsiEINEBuffer 4 + BSA50100101008060ABsiHKAINEBuffer 4 + BSA50100100100No65BBsiWINEBuffer 3100100100258055ABslINEBuffer 31050100758055ABsmAINEBuffer 4501001001008055BBsmBINEBuffer 3751001001008055BBsmFINEBuffer 4 + BSA2550501008065ABsmINEBuffer 475100751008065ABsoBINEBuffer 21010010050No37ABsp1286INEBuffer 4 + BSA2525251006537ABspCNINEBuffer 4 + BSA + SAM10075101008025ABspDINEBuffer 42575501006537ABspEINEBuffer 301010008037BBspHINEBuffer 410100501006537ABspMINEBuffer 3NRNR100NR6537BBspQINEBuffer 410501001008050BBsrBINEBuffer 4501001001008037ABsrDINEBuffer 2 + BSA5010050758065ABsrFINEBuffer 410100100100No37CBsrGINEBuffer 2 + BSA25100101008037ABsrINEBuffer 3050100108065BBssHIINEBuffer 3100100100 dd1008050ABssKINEBuffer 3 + BSA050100508060ABssSINEBuffer 3 + BSA050100 dd108037BBstAPINEBuffer 4 + BSA251001001008060ABstBINEBuffer 4755025100No65ABstEIINEBuffer 3 + BSA507510075No60ABstNINEBuffer 2 + BSA1010010075No60ABstUINEBuffer 410010050100No60ABstXINEBuffer 3050100256537BBstYINEBuffer 250100751008060ABstZ17INEBuffer 4NRNR100100No37BBsu36INEBuffer 3 + BSA02510008037ABtgINEBuffer 3 + BSA25501001008037ABtgZINEBuffer 4 + BSA102501008060ABtsCINEBuffer 45010050100No50BBtsINEBuffer 4 + BSA10050501008055AEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentCac8INEBuffer 450751001006537BClaINEBuffer 4 + BSA1050501006537ACspCINEBuffer 4 + SAM10100101006537ACviAIINEBuffer 47525101006525BCviKI-1NEBuffer 41050251008037ACviQINEBuffer 3 + BSA7510010075No25BEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentDdeINEBuffer 375100100756537ADpnINEBuffer 4100100751008037BDpnIINEBuffer DpnIINRNR100 ddNR6537BDraINEBuffer 47575501006537ADraIIINEBuffer 3 + BSA10075100256537BDrdINEBuffer 4 + BSA2550101006537AEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentEaeINEBuffer 4100100501008037AEagINEBuffer 31025100106537CEagI-HFNEBuffer 42550101006537CEarINEBuffer 4100100501006537AEciINEBuffer 2 + BSA50100100506537AEcoNINEBuffer 4100100751006537AEcoO109INEBuffer 4 + BSA100100751006537AEcoP15INEBuffer 3 + BSA + ATP751001001006537AEcoRINEBuffer EcoRI dd1001001001006537CEcoRI-HFNEBuffer 41010001006537CEcoRVNEBuffer 3 + BSA5075100508037AEcoRV-HFNEBuffer 4251001001006537BEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentFatINEBuffer 21010050506555AFauINEBuffer 410050101006555AFnu4HINEBuffer 41025251006537AFokINEBuffer 4100100751006537AFseINEBuffer 4 + BSA1007501006537BFspINEBuffer 4107510100 dd6537CEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentHaeIINEBuffer 4 + BSA75100501008037AHaeIIINEBuffer 450100251008037AHgaINEBuffer 110075501006537AHhaINEBuffer 4 + BSA751001001006537AHincIINEBuffer 3 + BSA751001001006537BHindIIINEBuffer 25010010506537BHinfINEBuffer 475100751008037AHinP1INEBuffer 41001001001006537AHpaINEBuffer 4255010100No37AHpaIINEBuffer 11005010506537AHphINEBuffer 4NR7501006537BHpy166IINEBuffer 4100100501006537CHpy188INEBuffer 45025101006537AHpy188IIINEBuffer 4 + BSA100100101006537AHpy99INEBuffer 4 + BSA101001006537AHpyAVNEBuffer 4 + BSA100100251006537BHpyCH4IIINEBuffer 410050251008037AHpyCH4IVNEBuffer 11002510256537AHpyCH4VNEBuffer 45050251006537BEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentI-CeuINEBuffer 4 + BSA101001006537BI-SceINEBuffer I-SceI + BSA105050506537BEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentKasINEBuffer 4 + BSA2510001006537BKpnINEBuffer 1 + BSA10075050No37AEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentMboINEBuffer 4751001001006537AMboIINEBuffer 4100100501006537CMfeINEBuffer 47550101006537AMfeI-HFNEBuffer 47550101006537AMluINEBuffer 32575100506537AMlyINEBuffer 4 + BSA5050251006537AMmeINEBuffer 4 + SAMNRNRNR100 dd8037BMnlINEBuffer 4 + BSA75100501006537BMscINEBuffer 47575751006537BMseINEBuffer 4 + BSA75100751006537AMslINEBuffer 4100100251006537AMspA1INEBuffer 4 + BSA50100501006537BMspINEBuffer 475100501008037AMwoINEBuffer 3107510075No60BEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentNaeINEBuffer 410075101006537ANarINEBuffer 410075751006537ANb.BbvCINEBuffer 250100101008037ANb.BsmINEBuffer 325100100258065ANb.BsrDINEBuffer 21010025508065ANb.BtsINEBuffer 4 + BSA75100751008037ANciINEBuffer 41002510100No37ANcoINEBuffer 31001001001006537ANcoI-HFNEBuffer 450100101008037BNdeINEBuffer 475100751006537ANgoMIVNEBuffer 410050101008037ANheINEBuffer 2 + BSA100100101006537CNheI-HFNEBuffer 4 + BSA1001001008037CNlaIIINEBuffer 4 + BSA2525251006537BNlaIVNEBuffer 4 + BSA1010101006537BNmeAIIINEBuffer 4 + SAM101001008037BNotINEBuffer 3 + BSA050100256537CNotI-HFNEBuffer 4 + BSA25100251006537ANruINEBuffer 3010100106537ANsiINEBuffer 31075100258037ANspINEBuffer 2 + BSA10010001006537ANt.AlwINEBuffer 22510025508037ANt.BbvCINEBuffer 450100101008037ANt.BsmAINEBuffer 410050101006537ANt.BspQINEBuffer 31075100258050BNt.BstNBINEBuffer 3010100108055ANt.CviPIINEBuffer 425100501006537AEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentPacINEBuffer 1 + BSA10075101006537APaeR7INEBuffer 42510010100No37APciINEBuffer 35075100508037APflFINEBuffer 4 + BSA025251006537APflMINEBuffer 3 + BSA0100100506537APhoINEBuffer 3505010075No75API-PspINEBuffer PI-PspI + BSA0101010No65BPI-SceINEBuffer PI-SceI + BSA00006537BPleINEBuffer 4100100751006537APmeINEBuffer 4 + BSA050101006537APmlINEBuffer 1 + BSA100750756537APpuMINEBuffer 40250100No37BPshAINEBuffer 4 + BSA5075101006537APsiINEBuffer 410100101006537BPspGINEBuffer 47510050100No75APspOMINEBuffer 42525101006537APspXINEBuffer 40100101008037BPstINEBuffer 3 + BSA7575100508037CPvuINEBuffer 3 + BSA1075100108037BPvuIINEBuffer 2100100100100No37BPvuII-HFNEBuffer 402501008037BEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentRsaINEBuffer 4100100501006537ARsrIINEBuffer 42575101006537CEnzymetopSupplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentSacINEBuffer 1 + BSA10050101006537ASacI-HFNEBuffer 4 + BSA10050101006537ASacIINEBuffer 42575101006537ASalINEBuffer 3 + BSA0010006537ASalI-HFNEBuffer 4101001001006537ASapINEBuffer 4755001006537CSau3AINEBuffer 1 + BSA10050101006537ASau96INEBuffer 4501001001008037ASbfINEBuffer 4755001006537ASbfI-HFNEBuffer 4502501006537BScaINEBuffer 3NRNR100 ddNR8037AScaI-HFNEBuffer 4100100101006537BScrFINEBuffer 41001001001006537ASexAINEBuffer 4 + BSA10075501006537ASfaNINEBuffer 3075100256537BSfcINEBuffer 4 + BSA7550101006537BSfiINEBuffer 4 + BSA010010100No50CSfoINEBuffer 42510050100No37BSgrAINEBuffer 410050101006537ASmaINEBuffer 40001006525ASmlINEBuffer 4 + BSA257525100No55ASnaBINEBuffer 4 + BSA255025100 dd8037ASpeINEBuffer 4 + BSA75100251008037CSphINEBuffer 2100100501006537BSphI-HFNEBuffer 45025101006537BSspINEBu