生物化学A卷.doc

学院： 年级/班级： 姓名： 学号： 装 订 线 内 不 要 答 题大学年第一学期生物学类生物化学(部分双语)考试试卷（A）（时间120分钟）题号得分得分 得分评卷人复核人.Write down these enzyme-catalyzed reactions in structural formula（3 points for each item, 12 points in total，either in English or Chinese）1. Glutamic-pyruvic transaminase(GPT)2. Adenylate deaminase3. Acyl CoA dehydrogenase4. Aldolase 得分评卷人复核人II. 填空题（每空1分，共22分）1. 蛋白质溶液由于具有_和_，所以作为亲水胶体系统是相当稳定的。2. 醛缩酶属于_类酶。3. 真核生物mRNA的3末端有一段长约200个核苷酸残基组成的_， 5末端有一个特殊的_结构。4. 缺乏_能引起脚气病，其辅酶形式是_。5. 维生素D的活性形式是_, 儿童缺乏维生素D会导致_。6. 典型的呼吸链包括_和_两种。7. 脂肪酸生物合成的反应在_中进行，而其合成原料乙酰辅酶A则在线粒体中生成，通过_转运机制而到达合成部位。8. DNA连接酶催化_键的形成，其能量来源不同，大肠杆菌为_，动物为_。9. 肝脏合成一分子尿素消除了两个氨基，其中一个来自_, 另一个来自_。10. 氨酰tRNA合成酶利用_供能，在蛋白质生物合成中起_作用。11. 生成一碳单位的氨基酸为甘氨酸、蛋氨酸、组氨酸、_、_。得分评卷人复核人III. Multiple choice（1 point for each item, 20 points in total）（Directions: there are four choices in each item, but only one is correct, choose the correct one.）装 订 线 内 不 要 答 题1. The B-DNA structure found in solution is a A.left-handed double helix of antiparallel chainsB.right-handed double helix of antiparallel chainsC.left-handed zig-zag helix of antiparallel chains.D.right-handed double helix of parallel chains.生物化学试卷共8页第1页 生物化学试卷共8页第2页2. Which of the statements regarding RNA is false? A.RNA contains A, C, G, and U.B.RNA is 3-5 linked.C.RNA has ribose.D.RNA is not hydrolyzed by OH-. 3. Which of the following is not a sensible grouping of amino acids based on their polarity properties? A.Ala, Leu, and Val.B.Arg, His, and Lys.C.Asp, Ile, and Pro.D.Asn, Ser, and Thr.4. In -pleated sheet structures, A.neighboring chains lie in a flat plane. B.neighboring residues are hydrogen bonded. C.neighboring chains are connected by a-helices.D.neighboring chains are hydrogen bonded.5. Which of the following is the most correct one？ A.Charged amino acids are never buried in the interior of a protein.B.Charged amino acids are seldom buried in the interior of a protein.C.All hydrophobic amino acids are buried when a protein folds.D.Tyrosine is only found in the interior of proteins.6. Allosteric effects that occur in hemoglobin A.only occur in humans.B.are important for maintaining Fe in the Fe2+ state.C.minimize oxygen delivery to the tissues.D.optimize oxygen delivery to the tissues.7. When S = Km, the velocity of an enzyme catalyzed reaction is about A.0.1Vmax.B.0.2Vmax.C.0.3Vmax.D.0.5Vmax.装 订 线 内 不 要 答 题8. Which of the following is correct concerning the uncoupling of electron transport from oxidative phosphorylation A.uncoupling slows all aerobic metabolic activities.B.uncoupling slows the citric acid cycleC.stops electron transportD.stops ATP synthesis.9. The rate of oxidative phosphorylation.is regulated by A. the ATP:ADP ratio.B.the concentration of acetyl CoA.C.the rate of flow of electrons through the electron-transport chain D. feedback inhibition by H2O.10. Molecules of NADH, FADH2 and GTP produced by one cycle of the citric acid cycle are A. 3, 2, 0B. 4, 2, 1C. 3, 1, 1D. 2, 2, 211. In glycolysis, substrate level phosphorylation is catalyzed by A. hexokinaseB. glyceraldehyde 3-phosphate dehydrogenaseC. phosphofructokinaseD. phosphoglycerate kinase12. Gluconeogenesis takes place predominantly in the A. muscles B. brainC. heart D. liver生物化学试卷共8页第3页 生物化学试卷共8页的第4页装 订 线 内 不 要 答 题13. What are the most important products that cells generate by means of the pentose phosphate pathway? A. Lactate and ATPB. Ribose-5-phosphate and NADPHC. NADP+ and ribose-5-phosphateD. NADPH and UDP-ribose14. What is the substrate of glycogen synthase? A. Glucose-1-phosphateB. UDP-glucoseC. Glucose-6-phosphateD. Glucose15. Fatty acid synthesis A.occurs in the mitochondriaB.needs NADH and ATPC.needs NADPH and ATPD.is a simple reversal of -oxidation16. General properties of the genetic code : A. degeneracy, wobbleB. universalityC. directory, continuous and non-overlappingD. all of the above are correct17. Which coenzyme is involved in amino acid transamination A.PLPB.Coenzyme B12C.NADP+D.NAD+ 18. Biosynthetic pathways that require NADPH include which of the following? A.GluconeogenesisB.Fatty acid biosynthesis.C.Ketone body formation.D.Tyrosine biosynthesis.19. In nucleotide metabolism, all of the following are true except A.Needs AAs,CO2 B.Both purine and pyrimidine biosynthesis are regulated by end-product inhibition.C.Needs cAMPD.De novo pyrimidine synthesis begins with a molecule of PRPP.20. DNA replication is a process of .A. conservative B. non-conservative C. semi-conservativeD. continuous得分评卷人复核人IV. Term interpretation（3 points for each item, 18 points in total, either in English or Chinese）1. Secondary structure of protein2. Competitive inhibition3. Signal sequence4. Semidiscontinuous replication 生物化学试卷共8页第5页 生物化学试卷共8页的第6页5. Operon6. Respiratory chain得分评卷人复核人V. 简答题（共28分）1. 称取25mg枯草杆菌蛋白酶粉剂，配制成25ml酶液。从中取出0.1ml酶液，以酪蛋为底物，用Folin酚比色法测定酶活力，得知每小时产生1500 ug酪氨酸。另取2ml酶液，用凯氏定氮法测得蛋白氮为0.2mg。若以每分钟产生1ug酪氨酸的酶量为1个活力单位计算，根据以上数据，求出：（1）1ml酶液中所含的蛋白质量及活力单位（2分）（2）比活力（2分）（3）1g酶制剂的总蛋白含量及总活力（2分）2. 请简述葡萄糖6磷酸可与哪些代谢途径相关联。（可用图式）（6分