dysadherin在肿瘤中的表达及其意义.doc

? dysadherin ? 2002Ino1Ecadherindysadherindysadherindysadherin,dysadherindysadherindysadherin? 1? dysadherin? dysadherincDNA178O12dysadherin312dysadherinTdysadherin cDNAPLC/PRF/5dysadherindysadherin1? 2? dysadherin? dysadherinactin organizationEcadherindysadherinCCL2dysadherin? 2.1? dysadherin ? dysadherin13? Shimamura3dysadherindysadherin(siRNAsmall interfering RNA)Panc1dysadherindysadherindysadherindysadherinCapan16dysadherindysadherindysadherindysadherin cDNAsiRNAdysadherindysadherinSCIDdysadherinCapan1SCID35 dCapan1mocktransfectanteddysadherinCapan1? dysadherin? 2.2? dysadherinEcadherin? dysadherinEcadherinIno 1dysadherindysadherin cDNAPLC/PRF/5mRNAdysadherinWestern blotdysadherin cDNAdysadherin cDNAEcadherinNorthern blotsdysadherin cDNAEcadherin dysadherinEcadherinEcadherin mRNA611dysadherinEcadherindysadherinEcadherin1? ,Ino 1 dysadherin cDNAPLC/PRF/5Ecadherin13dysadherinEcadherinEcadherin1? dysadherinOEcadherinTsuiji2dysadherinOOdysadherinEcadherindysadherinObenzylGalNAcdysadherindysadherinOdysadherinEcadherinOEcadherindysadherinOEcadherin? 4591011 dysadherinEcadherin3dysadherinEcadherinEcadherindysadherinEcadherindysadherin? 2.3? dysadherin? dysadherinCCL2Nam 14EcadherindysadherinEcadherin,EcadherindysadherindysadherinMDAMB231dysadherinCCL2dysadherinNFB(nuclear factorB pathway)CCL2dysadherinCCL2CCL2MDAMB231,dysadherinCCL2the prometastatic effect? 3? dysadherin3.1? ? dysadherinShimamura4dysadherinmAb NCCM53125dysadherindysadherindysadherindysadherin1250 20%2321% 50%3851% 100%640 20%dysadherin41.621% 50%22.251% 100%11.30 20%dysadherin7/23511 dysadherin0 20%dysadherin21% 50%51% 100%dysadherinEcadherin50% 100% dysadherinEcadherin9.10 50%dysadherinEcadherin31.7dysadherinEcadherin? 3.2? ? dysadherinShimada5276dysadherindysadherindysadherinEcadherindysadherinEcadherindysadherinEcadherindysadherin3.3? ? Sato692511031dysadherindysadherin10dysadherindysadherindysadherin5112dysadherin2874dysadherin12193dysadherin,dysadherindysadherinpTNMpT4dysadherinpT3pT2pT1(P0.001)dysadherindysadherinpN1pN0P0.001M1dysadherinM0P0.011dysadherindysadherin? 3.4? ? Wu7206dysadherindysadherindysadherindysadherindysadherindysadherinP0.04FIGOdysadherin? 3.5? ? dysadherinAoki882dysadherin51%50%dysadherindysadherinP0.003P0.0099P0.0036dysadherinEcadherinP0.0043,P0.0028dysadherin? 3.6? ? 91011dysadherindysadherin? 4? ? ? dysadherindysadherindysadherindysadherindysadherin? 1INO Y,GOTOH M,SAKAMOTO M,et al.dysadherin,a cancerassociated cell membrane glycoprotein,downregulates Ecadherin and promotes metastasisJ.Proc Natl Acad Sci U S A,2002,99(1):365370.2TSUIJI H,TAKASAKI S,SAKAMOTO M,et al.Aberrant Oglycosylation inhibits stable expression of dysadherin,a carcinomaassociated antigen,and facilitates cell adhesionJ.Glycobiology,2003,13(7):521527.3SHIMAMURA T,YASUDA J,INO Y,et al.dysadherin expression facilitates cell motility and metastatic potential of human pancreatic cancer cellsJ.Cancer Res,2004,64(19):69896995.4SHIMAMURA T,SAKAMOTO M,INO Y,et al.dysadherin overexpression in pancreatic ductal adenocarcinoma reflects tumor aggressiveness: relationship to Ecadherin expressionJ.J Clin Oncol,2003,21(4):659667.5SHIMADA Y,YAMASAKI S,HASHIMOTO Y,et al.Clinical significance of dysadherin expression in gastric cancer patientsJ.Clin Cancer Res,2004,10(8):28182823.6SATO H,INO Y,MIURA A,et al.dysadherin: expression and clinical significance in thyroid carcinomaJ.J Clin Endocrinol Metab,2003,88(9):44074412.7WU D,QIAO Y,KRISTENSEN G B,et al.Prognostic significance of dysadherin expression in cervical squamous cell carcinomaJ.Pathol Oncol Res,2004,10(4):212218.8AOKI S,SHIMAMURA T,SHIBATA T,et al.Prognostic significance of dysadherin expression in advanced colorectal carcinomaJ.Br J Cancer,2003,88(5):726732.9TAMURA M,OHTA Y,TSUNEZUKA Y,et al.Prognostic significance of dysadherin expression in patients with non small cell lung cancerJ.J Thorac Cardiovasc Surg,2005,130(3):740745.10NISHIZAWA A,NAKANISHI Y,YOSHIMURA K,et al.Clinicopathologic significance of dysadherin expression in cutaneous malignant melanoma: immunohistochemical analysis of 115 patientsJ.Cancer,2005,103(8):16931700.11NAKANISHI Y,AKIMOTO S,SATA Y,et al.Prognostic significance of dysadherin expression in tongue cancer: immunohistochemical analysis of 91 casesJ.Appl Immunohistochem Mol Morphol,2004,12(4):323328.12KYZAS P A,STEFANOU D,BATISTATOU A,et al.dysadherin expression in head and neck squamous cell carcinoma: association with lymphangiogenesis and prognostic significanceJ.Am J Surg Pathol,2006,30(2):185193.13RIMM D L,KOSLOV E R,KEBRIAEI P,et al.1(E)catenin is an actinbinding and bundling protein mediating the attachment of Factin to the membr