内科学硕士论文：糖尿病肾病 胰岛素 核因子-κB 单核细胞趋化蛋白-1 2型糖尿病大鼠.doc

内科学硕士论文：外源性胰岛素对2型糖尿病大鼠肾保护作用及肾组织NF-B、MCP-1表达的影响【中文摘要】观察外源性胰岛素对2型糖尿病(type 2 diabetes mellitus, T2DM)大鼠血核因子-B(nuclear factor-B, NF-B)、MCP-1(monocyte chemoattractant protein-1, MCP-1)的水平、尿MCP-1的排泄和肾组织NF-B、MCP-1的表达及对肾脏结构、功能的影响,探讨外源性胰岛素对T2DM大鼠的肾脏保护作用及机制。方法40只健康清洁级SD雄性大鼠,随机选10只作为正常对照组(A组)喂饲常规饲料,其余30只喂饲高糖高脂膳食、腹腔注射小剂量链脲佐菌素(streptozotocin,STZ)的方法建立T2DM大鼠模型,将成模的T2DM大鼠随机分为糖尿病组(B组)、优降糖治疗组(C组)、外源性胰岛素治疗组(D组)三个组,继续予以高糖高脂饲料喂养,其中C组每天以优降糖(5mg/kg)灌胃给药,A组、B组和D组灌以等量的生理盐水,D组予长效胰岛素皮下注射(2-8U/kg),根据血糖调整用量。药物干预第8周后收集12小时尿,并处死大鼠,收集血检测血MCP-1(serum monocyte chemoattractant protein-.【英文摘要】To observe the effect of exogenous Insulin on the serum Nuclear factor-B (NF-B) and monocyte chemoattractant protein-1(MCP-1) level, MCP-1 excretion in urine, the renal expression of NF-B and MCP-1 and the effect on renal constitution and function which prompts the investigation of its possible renoprotective mechanisms in type 2 diabetic (T2DM) rats.Method 40 healthy male SD rats of clean grade were chosen. 10 of them were randomly selected as normal control group (group A) which were fed .【关键词】糖尿病肾病 胰岛素 核因子-B 单核细胞趋化蛋白-1 2型糖尿病大鼠【英文关键词】Diabetic Nephropathy Insulin Nuclear factorB Monocyte chemoattractant protein-1 Type 2 diabetic rats【目录】外源性胰岛素对2型糖尿病大鼠肾保护作用及肾组织NF-B、MCP-1表达的影响中英文缩写对照表5-7中文摘要7-10Abstract10-131 引言14-152 材料与方法15-