组织培养主要培养基的种类与组成.doc

主要培养基的种类与组成一.White培养基二、LS培养基RM-64三.HB培养基四MS培养基RM62五. 花肥培养基六KC培养基 七. Kassanis 培养基八.木本植物用培养基九.Nitsch &Nitsch (1969)培养基十.Nielsen(1960)培养基十一.MT培养基十二.Miller 培养基(1963)十三.B5培养基十四.N6 培养基十五.H培养基十六. 改良White培养基一.White培养基, White(1963)1. 无机盐类Ca(NO3)24H2O 287mg/LKNO3 80mg/LKCl 65 mg/LNaH2PO4H2O 19.1 mg/LMgSO47H2O 738 mg/LNa2SO4：10H2O 453 mg/LMnSO44H2O 6.6 mg/LH3BO3 1.5 mg/LZnSO47H2O 2.7 mg/LKI 0.75 mg/L2有机物甘氨酸 3.0 mg/L烟酸 0.5 mg/L维生素B6 0.1 mg/L维生素B1 0.1 mg/L柠檬酸 2.0 mg/L蔗糖 20g/LpH 57二、LS培养基RM-64Linsmair & SKoog(1964) 1无机盐NH4N03 l 650mgLKNO3 1900 mg/LCaCl22H2O 440 mg/LMgSO47H2O 370 mg/LKH2PO4 170 mg/LNa2一EDTA 37.3 mg/LFeSO47H2O 27.8 mg/LH3BO3 6.2 mg/L MnSO44H2O 22.3 mg/LZnSO47H2O 8.6 mg/LKI 0.83 mg/LNa2MoO2H2O 0.25 mg/LCuSO45H2O 0.025 mg/LCoCl26H2O 0.025 mg/L2有机物维生素Bl 04 mg/L肌醇 100mgL蔗糖 30gLpH 56 * FeSO47H2O 557g，Na2一EDTA 745g先溶于1升蒸馏水里，然后按每升培养基含5mg的比例加入培养基中。三.HB培养基 Holley &Baker(1963)1. 无机盐(以1/2浓度的Knop液为基础)Ca(NO3)2.4H2O 1000mg/LKNO3 125 mg/LMgSO47H2O 125mg/LKH2PO4 125 mg/LBerthelots液 0.5ml/LBerthelots液成分组成:MnSO44H2O 20g/LKI 0.5g/L NiCl26H20 0.05 g/LCoCl26H20 0.05g/LZnSO47H20 0.10 g/LCuSO45H2O 0.05g/LBeSO44H2O 0.10 g/LH3BO3 0.05g/L浓硫酸 1ml2. 有机物维生素B1理学 1 mg/L腺嘌呤 8 mg/LNAA 2 mg/L蔗糖 40gL 四MS培养基RM62(Murashige & Skoog (1962)1.无机盐类同LS培养基 2有机物五. 花肥培养基Kano(1963)(Kyoto so1ution)花肥 3g/LKH2PO4 250 mg/LCa(NO3)2.4H2O 1000mg/LMgSO47H2O 250 mg/L(NH4)2SO4 2mg/L蔗糖 20gL pH 5.0六KC培养基 Knudson C(1964)1.无机盐KH2PO4 250 mg/LCa(NO3)2.4H2O 1000mg/L(NH4)2SO4 500mg/LMgSO47H2O 250 mg/LFeSO47H2O 25 mg/LMnSO44H2O 7.5 mg/L2.有机物蔗糖 20gL pH 5.05.2 七. Kassanis 培养基(1957)1.无机盐Ca(NO3)2.4H2O 500mg/LKNO3 125 mg/LMgSO47H2O 125mg/LKH2PO4 125 mg/LBerthelots液 10滴2. 有机物酪蛋白 1 mg/L半胱氨酸 10 mg/L腺嘌呤 5 mg/L烟酸 1 mg/L维生素B6 1 mg/L泛酸钙 10 mg/L肌醇 0.1 mg/L维生素H(生物素) 0.01 mg/L蔗糖 20 gL八.木本植物用培养基(WPM,Woody Plant medium)1.无机盐NH4N03 400mgCa(NO3)2.4H2O 556mg/LK2SO4 990 mg/LCaCl22H2O 96 mg/L KH2PO4 170 mg/LNa2MoO42H2O 0.25 mg/LMgSO47H2O 370 mg/LMnSO4H2O 22.4mg/LZnSO47H2O 8.6mg/LCuSO45H2O 0.25mg/LFeSO47H2O 27.8 mg/LNa2-EDTA 37.3 mg/L2.有机物肌醇 100 mg/L维生素B1 1.0mg/L烟酸 0.5 mg/L维生素B6 0.5 mg/L甘氨酸 2.0 mg/L蔗糖 20 gL琼脂 6gLpH 5.2九.Nitsch &Nitsch (1969)培养基1. 无机盐KNO3 950 mgLNH4N03 720mgLKH2PO4 68 mg/LCaCl22H2O 166 mg/LMgSO47H2O 185mg/LFeSO47H2O 27.8 mg/LNa2-EDTA 37.3 mg/LMnSO44H2O 25mg/LH3BO3 10 mg/LZnSO47H2O 10mg/LCuSO45H2O 0.025mg/LNa2MoO42H2O 0.25 mg/L2.有机物维生素H(生物素) 0.05 mg/L肌醇 100 mg/L维生素B1 0.5mg/L烟酸 5 mg/L维生素B6 0.5 mg/L甘氨酸 2.0 mg/L叶酸 5 mg/L蔗糖 20 gL十.Nielsen(1960)培养基1.无机盐KNO3 200 mgLCa(N03)24H2O 800mgL MgSO47H2O 200mg/LKH2PO4 200 mg/LZnSO47H2O 0.2mg/LNiCl26H2O 0.3 mg/LMnCl2H2O 1.8 mg/LCuSO45H2O 0.08mg/LH2MoO4H2O 0.02 mg/LH3BO3 2.8mg/L2.有机物维生素B1 1mg/L烟酸 5 mg/L维生素B6 1 mg/L蔗糖 30 gL十一.MT(murashge and Tucker)培养基1.大量元素NH4N03 l 650mgLKNO3 1900 mg/LCaCl22H2O 440 mg/LMgSO47H2O 370 mg/LKH2PO4 170 mg/LNa2一EDTA 37.3 mg/LFeSO47H2O 27.8 mg/LH3BO3 6.2 mg/L MnSO44H2O 22.3 mg/LKI 0.83 mg/LNa2MoO2H2O 0.25 m g/LCuSO45H2O 0.025 mg/LCoCl26H20 0.025 mg/L2有机物维生素Bl 04 mg/L维生素B6 10.0mg/L烟酸 5 mg/L甘氨酸 2.0 mg/L肌醇 1000mgL蔗糖 50gL十二.Miller 培养基(1963)1.无机物NH4N03 l 000mgLKNO3 1000 mg/LCa(N03)24H2O 347mg/L KH2PO4 300 mg/LKCl 65 mg/LMgSO47H2O 35 mg/LNa一Fe一EDTA 32 mg/LMnSO44H2O 4.4 mg/LZnSO47H2O 1.5mg/LH3BO3 1.6 mg/L KI 0.8 mg/LCaCl22H2O 150 mg/L2有机物维生素Bl 0.1 mg/L维生素B6 0.1mg/L叶酸 0.5 mg/L甘氨酸 2.0 mg/L蔗糖 30gLpH 6.0十三.B5培养基(Gamborg 等,1968)1.无机物NaH2PO4H2O 150 mg/LKNO3 3000 mg/L(NH4)2SO4 134mgLMgSO47H2O 500 mg/LNa2一EDTA 37.3 mg/LFeSO47H2O 27.8 mg/LMnSO4H2O 10 mg/LZnSO47H2O 2mg/LH3BO3 3mg/L Na2MoO2H2O 0.25 m g/LCuSO45H2O 0.025 mg/LCoCl26H20 0.025 mg/LKI 10 mg/L2.有机物维生素B1 10mg/L维生素B6 1 mg/L甘氨酸 2.0 mg/L叶酸 0.5 mg/L肌醇 1000mgL蔗糖 50 gLpH 5.5十四.N6 培养基(北京植物所,黑龙江农业科学院,1974)1.无机物KNO3 2800 mg/L(NH4)2SO4 463mgLKH2PO4 400 mg/LMgSO47H2O 185 mg/LCaCl22H2O 165 mg/LNa2一EDTA 37.3 mg/LFeSO47H2O 27.8 mg/LMnSO4H2O 4.4 mg/LZnSO47H2O 1.5mg/LH3BO3 1.6mg/L KI 0.8mg/L2.有机物维生素B1 1mg/L维生素B6 0.5 mg/L叶酸 1 mg/L蔗糖 20 gLpH 5.8十五.H培养基1.无机盐KNO3 950 mg/LNH4N03 720mgLMgSO47H2O 185 mg/LCaCl22H2O 166 mg/LKH2PO4 68 mg/LNa2一EDTA 37.3 mg/LFeSO47H2O 27.8 mg/LMnSO44H2O 25 mg/LZnSO47H2O 10mg/LH3BO3 10mg/L Na2MoO2H2O 0.25 m g/LCuSO45H2O 0.025 mg/L2.有机物维生素B1 0.5mg/L维生素B6 0.5 mg/L甘氨酸 2.0 mg/L叶酸 0.5 mg/L肌醇 1000mgL烟酸 0.5 mg/L生物素 0.05 mg/L蔗糖 20 gLpH 5.5十六. 改良White培养基(White,1963)1.无机盐KNO3 80 mg/LCa(N03)24H2O 300mgLMgSO47H2O 720 mg