世界针灸学大会报告：方便揿针原始效应物质研究

针刺原始效应物质的研究 Study on Acupuncture Original Material世界针灸学会联合会成立20周年庆典暨世界针灸学术大会讲演稿,张大同 杨肖娥1 王樟连2 郑振洨 王海明 李廷强1 冯英1 沈瑾 王芸2（杭州市第三医院，杭州 310002 ；1 浙江大学，杭州 310027；2 浙江中医药大学，杭州 310053） (ZHANG datong,YANG Xiaoe1, WANG Zhanglian2, ZHENG Zhenxiao, WANG Haiming, LI Tingqiang1, FENG Ying1, SHEN Jin ,Wang Yun2 （Third Hospital of Hangzhou, 310002；1 Zhejiang University, 310027； 2 Zhejiang Chinese Medical University 310053, Hangzhou, Zhejiang Province，P R .China ）,目的 将下述4项实验所取得的证据及电化学、微观腐蚀原电池效应科学规律，共同组成一个完整的证据锁链，证明针行方便揿针针刺原始效应物质、针刺电化学效应机制。,Objective We will practice the following Four experiments made by the evidence, electrochemical and corrosion original micro-battery effect scientific laws, the common composition of a complete chain of evidence ，and will prove that the effect of acupuncture raw materials, electrochemical effects of acupuncture mechanism.,实验1 针行方便揿针针刺电化学电流研究实验1-1 人体针刺电化学电流研究,Experiment 1-1 Electrochemical current study by acupuncture,方法 将针行方便揿针分别垂直刺入实验对象的肩髃、肩髎穴位中，用数字显示电流仪测量记录产生的电流变化,Methods Acupuncture needles were stung vertically to alienate experiments of the points of Jianyu, Jian liao, with figures current measurement records showed the changes of the current .,结果 针行方便揿针刺入人体穴位，所测的9名对象电流变化在-44 67A区间，电流差值为27 73A。,Result Stainless steel acupuncture were needled into the human body , measuring the changes on the current target is -44 67 uA interval, the current margin was 27 73 A on the cave of 9 cases .,表1-1、针刺肩髃、肩髎穴的电流测定记录表（男=m、女=w）Table 1、 Statistics of electric current measured in the point of Jian Yu、Jian liao inserted（Man=m、Woman=w）,N 性别 年龄（岁） 电流变化（A） 电流差值（A） 峰值出现（次） Sex age（years） electric current electric current difference peak value 1 w 69 -2013 33 42 m 52 -943 52 53 w 65 -4418 62 54 m 78 -3716 53 6 5 w 75 -423 27 46 w 77 -667 73 47 m 72 -2448 72 58 w 54 -1239 51 59 m 23 -2547 72 6,实验1-2 动物针刺电化学电流研究,方法 将12只ICR实验小鼠按其存活及处死（强力颈椎脱位法）后的整个时间过程分为：A组（健康活体），B组（死亡1 h），及C组（死亡28 h）；先将针行方便揿针分别刺入每只经麻醉的ICR实验小鼠左侧的曲池与后三里穴，用数字显示电流仪测量观察2min并记录产生的电流变化，后将该小鼠处死；待该小鼠处死1 h、 28 h后再分别对其右侧的曲池与后三里穴，并用数字显示电流仪测量观察2min并记录产生的电流变化；记录的数据再制表分析。,结果 针刺实验小鼠A组的所发电流在83 1A区间，并一直呈现由高向低的电流变化，电流发生率为100，电流的平均差值为35.92A； B组所发电流在41 -1A区间，并一直呈现由高向低的电流变化，电流发生率为100，电流平均差值为21.58A； C组所发电流在148 1A区间，一直呈现由高向低的电流变化，电流发生率为100，电流平均差值为68.92A。,表1-2、12只小鼠活时及死后针刺曲池、足三里穴所发电流发生率分析表（A）,No 体重g A组 A组差值 B组 B组差值 C组 C组差值1 19.4 286 22 2402412037 832 18.5 214 17 2332010243 593 19.1 362 34 9-11012367 564 21.2 369 27 34232438 355 18.3 152 13 167095913 466 20.0 832 81 383359618 787 18.3 588 50 416353812 268 19.7 344 30 3113014840 1089 17.9 618 53 122101121 11110 20.0 591 58 24222893 8611 17.3 233 20 1101110428 7612 18.5 282 26 265219330 63合计 228.2 831 431 41 -1 259148 1 827均数 19.02 35.92 21.58 68.92发生率 100 100100,分析 证明该实验所测电流为针刺电化学效应的依据如下：,Analysis The current experiment provides for the evidence on the effects of acupuncture electrochemical.,A、由于无皮肤的组织电阻为300800，因此实验1-1所测到的-44 67A瞬间电流变化区间的电流差值为27 73A。而其瞬间电压变化区间（根据欧姆定律）应为：8.1V58.4V，明显远远大于50100mV的细胞动作电位；因此该瞬间电流不是我们已知的生物内在电流。而实验1-2所测的83 1A、41 -1A、148 1A瞬间电流变化区间的平均电流差值为35.92A、21.58A、21.58A，并一直呈现由高向低的电流变化，电流发生率为100，应与上述实验1-1的结果相同。,A、 The organization skin resistance was 300 to 800 cm on without skin, so the margin of transient current changes was 27 73 A on the interval -44 67 A. While the instantaneous voltage (according to Ohms law) was 8.1 V 58.4V, obviously far greater than the 50 100 mV of action potentials；As such, the current was not biological internal currents as we know。,B、此外，笔者曾在2002年的离体动植物（猪肉、草莓）电化学测定实验中也明显证实了（结果与此次活体实验一样）其间也能产生uA级的电流。,B、In addition ，the result also was proved by in vitro electrochemical plants and animals of the experiment in 2002 (with the same vivo experiments) which could also have a Class A A Electricity.,C、针行方便揿针电化学科学规律。,C、The existing electrochemical universal laws of science.,实验1 结论 针刺穴位组织（能改变电位差）具有电化学原电池效应。,Experiment 1 Conclusion Acupuncture points (to change the potential difference) have the original battery electrochemical effect.,实验2 方便揿针针体的腐蚀研究,Experiment 2 Clinical studies of acupuncture needles corrosion（ The control group did not join because of original limited to the length, it was added as follows）,方法 将3600枚针行方便揿针随机分成实验组（3000枚）和对照组（600枚），在一年中，实验组的3000枚针行方便揿针在临床反复灭菌使用，对照组600枚针行方便揿针也随实验组一起反复灭菌但不使用，其间由目测挑选出具有可见腐蚀斑的揿针（数据采用x2检验处理，P0.05有统计学意义），再选典型腐蚀的揿针用电子显微镜放大拍摄分析。,Methods 3,600 acupuncture needles were divided into the experimental group (3000) and the control group (600) randomly. 3000 acupuncture needles were used repeatedly by sterilization, and 600 acupuncture needles were sterilized again and again as the experimental group but without using，too. Meantime, selected corrosion spot with the acupuncture needle by the visual visible (data processing using x2 test, P 0.05 was significantly), the re-election of typical corrosion acupuncture needles to be amplified with electronic microscope.,结果 目测出实验组腐蚀针259枚（电子显微镜：腐蚀针行针灸针表面呈现部分较严重腐蚀缺损区域），而对照组目测腐蚀针为0枚；实验组与对照组比较， P0.05差异有显著意义。请见具体资料。,Result 259 needles were corroded in the experimental group (electron microscope: acupuncture needle surface corrosion showed some of the more serious corrosion defect regions), while 0 needle was corroded in the control group by eyeballing; The result of experimental group compared with the control group, P 0.05 significant difference. See specific information.,表2、实验组针行方便揿针针体目测腐蚀率发生分析表Table 2、The experimental group stainless steel acupuncture needles visual analysis of corrosion rate in table,时间（月） 腐蚀针数 未腐蚀针数 分析总针数 腐蚀百分率Time（A month） Corrosion pin counts No corrosion pin counts The total number of needle Corrosion percentage13 0 3000 3000 046 19 2981 3000 0.6379 51 2930 2981 1.711012 189 2741 2930 6.45Total 259 2741 3000 8.63,表3、实验组与对照组目测针行方便揿针腐蚀率的比较Table 3、The experimental group and control group visual stainless steel acupuncture needle corrosion rate comparison,腐蚀针数 未腐蚀针数 分析总针数 腐蚀百分率 Corrosion pin counts No corrosion pin counts The total number of needle Corrosion percentage实验组 259（8.63） 2741（91.37） 3000 8.63(The experimental group)对照组 0（0） 600 （100） 600 0(The control group)Total 259 3341 3600 7.19x 2=55.81562406 P0.05,腐蚀揿针的电子显微镜照片所示腐蚀现象 见图1、2、3,Corrosion acupuncture needle shown in the electron microscope photographs of corrosion phenomenonSee figure 1, 2, 3,图1 放大50倍的腐蚀针行方便揿针局部Figure 1 Large 50 times the local corrosion acupuncture needles,图2 放大100倍的腐蚀针行方便揿针局部Figure 2 Large 100 times the local corrosion acupuncture needles,图3 放大1000倍的腐蚀针行方便揿针局部Figure 3 Large 1000 times the local corrosion acupuncture needles,实验2 结论 针行方便揿针针刺穴位组织会使针体腐蚀，该腐蚀现象符合电化学腐蚀原理。,Experiment 2 ConclusionThe point of acupuncture needles willcorrode the acupuncture needles , corrosion phenomenon is consistent with the principle of electrochemical corrosion.,实验3 针行方便揿针针刺穴位组织铬、镍、铁元素含量变化的研究,Experiment 3 The study on the change of element of acupuncture Points with chromium, nickel, iron.,方法 将15只实验兔按A（揿针10枚刺入左环跳穴区电针仪通电留针5h）、B（揿针10枚刺入右臂臑穴区留针5h）实验组及C（右环跳穴区）、D（右臂臑穴区）对照组，进行实验，5h后对60个（每点15个）样本用(ICP-MS)电感耦合等离子体质谱仪（对与方便揿针主体相关联的铬、镍、铁元素的含量）进行分析，数据采用t检验处理，P0.05有统计学意义。,Methods 15 rabbits were divided into the experimental groups and control groups ，the experimental groups were A (10 acupuncture needles piercing left Central Point district jump-electro-acupuncture needle telegram to stay 5 h) ，and B (10 acupuncture needles piercing right arm HDL Points district to stay 5 h). The control groups were C (right ring jump Point), and D (right arm HDL Point district. 60 samples (15 per point) were analyzed by electricity flu coupled plasma mass spectrometer (with stainless steel acupuncture needles linked to the main chromium, nickel, iron content), data processing using t-test, P 0.05 with statistical significance.,结果 实验组铬、镍、铁元素含量大幅升高，与对照组比较，P0.001其差异具显著意义。见表4、表5。,Result The experimental group chromium, nickel, iron content significantly increased, compared with the control group, P 0.001 with its significant difference. See Table 4 and Table 5.,表4 两组臂臑穴区铬、镍、铁元素含量的比较 （S ，ng/g，各组n=15）Table 4、Compare of number of Cr、Ni、Fe in the point of Jian Yu between two groups,铬Cr 镍Ni 铁Fe实验组（留针） 270.0168271.63 116.1608743.87 27060.80411332.91 (stay needle)对照组Control 54.00709012.76 2.73888721.949 5523.45362380.729 Compared with the control group（与对照组比较）: P0.001,表5 两组环跳穴区铬、镍、铁元素含量的比较（S ，ng/g，各组n=15）Table 5、 Compared with the element of Cr、Ni、Fe in the point of Jian liao between two groups,铬Cr 镍Ni 铁Fe实验组（电针） 303.93884115.7 134.7799164.55 14175.5915327.276(Electroacupuncture) 对照组Control 58.01403019.39 5.31042624.824 5414.27642531.767Compared with the control group（与对照组比较）: P0.001,实验3 结论 针行方便揿针针刺穴位组织（能够增加离子、微量元素）具有电化学原电池效应。,Experiment 2 ConclusionAcupuncture points (to increase ions, trace elements) have the original electrochemical battery effect.,总体结论 按上述4项实验所取得的证据及电化学、微观腐蚀原电池效应科学规律，所共同组成的证据锁链。笔者认为：针行方便揿针针刺穴位组织的原始效应物质为电子、微量元素；针刺