生物化学2004期末考试题目

1、中南大学2003-2004学年第二学期考试试卷Exam paper for the 2n term of 2003-2004 at Central South UniversitySubject: BiochemistrySpecialty: BioengineeringCourse character RequiredExam mode： Without referenee documents but permission of any kind of dictionaryExam time len gth： 120 mi nu tesStude nt Name,Register No.Sc

2、orePart I True or False (1 point each,20 pts)Please write T (true) or F (false) in the bla nketi.2.3.4.5.6.7.9.10.11.12.Passive diffusi on n eeds en ergy ()Glucose tran sport in erythrocytes occurs by facilitated diffusi on ( All active tran sport systems are en ergy-coupli ng devices ()Glycolysis i

3、s a catabolism to degrade glucose ()Glycolysis provide the orga nism not only en ergy but also some importa nt in termediates ()TCA cycle is the major way to produce en ergy by aerobic orga ni sms ()Both photophosphorylatio n and oxdative phosphorylati on produce A TP ( )ATP is not the major en ergy

4、 form in the livi ng orga nism()ATP, acetyl-CoA and glucose-6-phosphate have high-e nergy bond.( NADH normally occurs in biosynthesis pathway, while NADPH biodegradatio n pathway ()TCA cycle produces ATP directly ()Carbohydrate produced by photos yn thesis occurs in the dark period (occurs primarily

5、in13.14.15.16.17.18.19.Lipid bilayer takes part in mass tran sfer, en ergy product ion and sig nal com muni cati on ( Biochemical react ion has not space but substrate specificati ons ()Degradatio n of lipid can provide much more en ergy and water tha n carbohydrates ( NADPH is n ecessary for mai nt

6、ai ning the reductive en viro nmen ts for blood cells ( Proli ne ofte n occurs in a termi nal and turn of :-helix of protein ()Alpha helix and beta-sheet of prote in is due to hydroge n bond, but sub un its of hemoglobuli n are arran ged due to hydrophobic force.()Change in con formati on is n ormal

7、ly accompa nied by breakage of chemical bon d(s)()Pre-mRNA tran scripted from DNA for prokaryotes no rmally does not n eed any process ing to form the fun ctio nal mRNA.()20.2Part II Multiple Choice (1 point each， 30 pts)Choose the one best answer to each of the following questions.1. Biochemical sy

8、nthesis of new cell material is calledA. MetabolismB. AnabolismC. CatabolismD. Synthatabolism2. TCA cycle is a pathway ofA. anabolismB. catabolismC. photosynthesisD. reductive pathway3. Chemoorganotrophs use which of the following as an energy source?A. Organic compoundsB. Inorganic compoundsC. Both

9、 organic and inorganic compoundsD. Light4. A fastidious organism isA. oxygen sensitiveB. temperature sensitiveC. nutritionally demandingD. atmospheric pressure sensitive5. If G 0 is positiveA. the reaction is exergonic and requires the input of energy.B. the reaction in endergonic and requires the i

10、nput of energy.C. the reaction is exergonic and energy will be released.D. the reaction is endergonic and energy will be released.6. Which statement is true?A. Coenzymes are generally bound tightly to their respective enzymes.B. Prosthetic groups are generally bound tightly to their respective enzym

11、es.C. Coenzymes and prosthetic groups are both bound tightly to their respective enzymes.D. Coenzymes and prosthetic groups are technically not bound to any enzymes.7. In an electron carrier system, the net energy change is determined by thedifference in reduction potentials between theA. primary el

12、ectron donor and the terminal electron donor.B. primary electron acceptor and the terminal electron acceptor.C. primary electron acceptor and the terminal electron donor.D. primary electron donor and the terminal electron acceptor.8. The Embden-Meyerhof Pathway is another name forA. the citric acid

13、cycleB. glycolysisC. electron transportD. NADH production9. In glycolysisA. the crucial product is ATP; the fermentation products are waste products.B. the crucial product is ethanol or lactate; ATP is a waste product.C. the crucial product is CO2; ATP is a waste product.D. the crucial product is no

14、t relevant because glycolysis is not a major pathway.10. In chemiosmosis+A. OH- accumulates on the outside of the membrane while H accumulates on the inside.B. OH- accumulates on the inside of the membrane while H + accumulates on the outside. +C. both OH- and H accumulate on the inside of the membr

15、ane.+D. both OH- and H accumulate on the outside of the membrane.11. Which statement is not true? Uncouplers prevent ATP synthesisA. by interfering with electron flow.B. by creating leaky membranes.C. without affecting electron transport.D. by destroying the proton motive force.12. The chemical subs

16、tance that enters the citric acid cycle for further metabolismisA. ethyl alcoholB. acetyl-CoAC. adenosine triphosphateD. pyruvic acid13. Which of the following occurs in the absence of oxygen?A. FermentationB. Anaerobic respirationC. Anoxygenic photosynthesisD. All of the above.14. Most chemolithotr

17、ophs areA. phototrophsB. autotrophsC. heterotrophsD. organotrophs15. For a carbon source, chemoorganotrophs generally use such compounds asA. glucoseB. carbon dioxideC. triglyceridesD. sunlight16. For each molecule of pyruvate that enters the citric acid cycle, how manymolecules of CO2 are generated

18、?A. 1B. 2C. 3D. 617. Whether an organism is classified as a photoheterotroph or a photoautorophdepends on itsA. energy source.B. carbon source.C. oxygen requirements.D. All of the above.18. How many ATP result from the transfer of electrons from NADH to O 2 throughthe electron transport chain?A. 1B.

19、 2C. 3D. 619. To describe a type of photosynthesis as oxygenic implies thatA. oxygen is producedB. oxygen is consumedC. oxygen functions as a catalyst20. The Calvin cycleA. is responsible for the fixation of CO 2.B. utilizes only ATP.C. requires phosphofructokinase and pyruvate decarboxylaseD. all o

20、f the above21. DNA is always synthesized in the direction.A. amino terminal to carboxy terminalB. carboxy terminal to amino terminalC. 3 to 5D. 5 - 5E. 5 - 322. The short pieces of DNA made in the discontinuous strand are called fragments?A. IntronB. CistronC. ExonD. PolycistronicE. none of these23.

21、 DNA replication begins at locations called _ sites.A. PromoterB. OriC. Shine-DelgaroD. OkazakiE. Intron24. All DNA polymerases need a to attach a nucleotide to.A. RNA primerB. LigaseC. 3-OHD. deoxynucleotideE. 5-triphosphate25. Which of the following is not a substrate for DNA polymerases?A. deoxya

22、de nosine triphosphateB. DNA templateC. a nu cleotide primer with a free 3-OHD. deoxyuracil triphosphateE. deoxygua nosine triphosphate26. Which of the following replaces the RNA primer with deoxynucleotides?A. DNA polymerase IB. DNA polymerase IIC. DNA polymerase IIID. DNA ligaseE. primase27. If Vm

23、ax=100 Mmol/mL sec and Km=2mM, the velocity of the reaction is,when S=20mMA. 108 -Mol/mL secB. 91 hol/mL secC. 67 一 mol/mL secD. 145 .lEol/mL sec28. The carboxylation catalyzed by a carboxylase usually needs a prothetic group2+A. FeB. biot inC. ferrocya ninD. Thiami ne29. In a typical cycle of beta-

24、degradation of C16 saturated fatty acids, it includes twosteps of oxidation, in which the electron carriers are respectively :+A. FAD and NAD+B. NAD and FADC. FAD and FAD+ +D. NAD and NAD30. In an erythrocyte undergoing glycolysis, what would be the effect of a suddenincrease in the concentration of

25、 ATPA. Glycolysis in hibitedB. glycolysis stimulatedC. fructose-1,6-bisphosphate accumulatedD. acetyl-CoA accumulatedPart III Filling Blankets (1 point each, 20 pts)Please fill each blanket with suitable word(s) either in English or Chin ese1. When exter nal electr on acceptors are not available in

26、ano xic en viro nmen ts, carb on is catabolizedby ().2. The two major products of pen tose phosphate pathway are () and()3. The major chemical energy forms from catabolism that are used for biosynthesis are () and ().4. There are six subclasses of enzymes according to the Enzyme Commission, please g

27、ive their systematic names (; ,; )5. The fates of pyruvate produced by glycolysis are :(1); (2)(3); (4)6. The energy source for the heterotrophs, phototrophs and lithotrophs correspondingly are (; ;).7. Besides glycolysis, please list the other two most important pathways:(; )Part IV Short essay que

28、stions (30 pts)Please answer the following short essay questions either in English or Chinese1. Please describe the structural characters of the four different levels of protein.(4 pts)162. Please compare and contrast fermentation and respiration. (4 pts)3. Chemiosmotic is used to explain the mechan

29、ism of phosphorylation (see the following figure). Please explain. (4 pts)Add ADP32P, and alkali-15 sec- i1It 亠啓一1=ATP, Y32Ppli MlabeledFollowed by alkalij1-JU 4VTFEE4. Please describe the regulation of the TCA cycle according to the following figure (4 pts)ATPPyruvateacetyl CoA.NADHOCH 3 C SCoANADH + CO 2Oxaloacetate草酰乙酸NADHCOONADCOOcooO=CCH2COO CitrateCH 2 柠檬酸HO C COOATP citrateHOCHCH 2COOMalate苹果酸CH2C