人类疱疹病毒优秀精美生物医学课件

1、人类疱疹病毒优秀精美生物医学 人人 类类 疱疱 疹疹 病病 毒毒Human herpes viruses人类疱疹病毒优秀精美生物医学nContains several of the most important human pathogensnA spectrum of diseasesnLife long persistent infectionnPeriodic reactivation人类疱疹病毒优秀精美生物医学nVirion: spherical, 150-200nm core + capsid + envelope dsDNA, encodes at least 100 protei

2、ns 35 proteins structure of virusnReplication: nIn the nucleusnBud from nuclear membranenEarly replication and late replication人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学nCPE: apart from EBV, others can cause CPE in cell culturenForm of infectionnReplication infection : primary, reactivatednLatent in

3、fectionnCongenital infection ( (先天性感染先天性感染) )nCell transformation人类疱疹病毒优秀精美生物医学 病毒病毒 infected cells diseasesHSV1粘膜上皮细胞感觉神经节细胞粘膜上皮细胞感觉神经节细胞多种疾病及潜伏感染多种疾病及潜伏感染HSV2粘膜上皮细胞感觉神经节细胞粘膜上皮细胞感觉神经节细胞生殖器疱疹及潜伏感染生殖器疱疹及潜伏感染VZV皮肤细胞脊髓后根神经节，颅神皮肤细胞脊髓后根神经节，颅神经的感觉神经节经的感觉神经节水痘带状疱疹水痘带状疱疹EBVB细胞，上皮细胞细胞，上皮细胞B细胞细胞传染性单核细胞增多症传染性单

4、核细胞增多症Burkitt瘤，鼻咽癌瘤，鼻咽癌CMV上皮细胞，白细胞，精子细胞上皮细胞，白细胞，精子细胞分泌腺体分泌腺体多种疾病多种疾病HHV6CD4+T细胞细胞婴儿丘疹（唾液传播）婴儿丘疹（唾液传播）免疫低下：持续急性感染免疫低下：持续急性感染HHV7CD4+T细胞细胞幼儿皮疹（唾液传播）幼儿皮疹（唾液传播）与其他疾病关系不清与其他疾病关系不清HHV8不清不清Kaposi肉瘤有关肉瘤有关人类疱疹病毒优秀精美生物医学nPropertiesnTwo serotypes : HSV1 and HSV2nHSV genome: encodes at least 70 proteins some as

5、sociated with viral replication some envelope proteins associated with immune reaction viral penetration人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学nSource of infection patients and carriersnRoutes of transmissionn HSV1： contactq HSV2： sexually / maternal genital infectionqPathology :cytolytic infection 人类疱疹病毒优秀精美生

6、物医学nPrimary infection most are inapparent , but result in antibody production and latent infection人类疱疹病毒优秀精美生物医学nHSV1 nGingivitis (齿龈炎齿龈炎): most frequentlynKeratoconjunctivitis ( (角膜结膜炎角膜结膜炎) )nSkin infectionnEncephalitisnLatent infection : in the trigeminal ganglia (三叉神经节三叉神经节)人类疱疹病毒优秀精美生物医学nHSV2nG

7、enital herpes: common seennSkin herps : as HSV1nNeonatal herpes : during birth, is a severe disease skin, eye, month lesion encephalitis multiple organ disseminated ( (多器官多器官感染感染) )nLatent infection：sacral ganglia (骶神经骶神经节节)人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学nLatent and recurrence infection H

8、SV1,HSV2 neuron virus reactivate , replication axon (神经纤维神经纤维) peripheral sites, skin or mucous membraneHSV1：口唇部疱疹：口唇部疱疹 HSV2：genital herpes人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学n similar as HSV 人类疱疹病毒优秀精美生物医学nPathogenicityn Source of infection：patientsn Routes of infection: respiratory tractn Disease：n 水痘水痘(

9、Varicella/chickenpox)(Varicella/chickenpox)：n 儿童：斑丘疹，水疱，偶见并发症儿童：斑丘疹，水疱，偶见并发症n 孕妇：症状较重，可致胎儿畸形，流产，死胎孕妇：症状较重，可致胎儿畸形，流产，死胎n 免疫低下：重症水痘免疫低下：重症水痘n Latent infection dorsal root ganglia ( (脊髓后根神经节脊髓后根神经节) )人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学nZoster (带状疱疹带状疱疹)nSeen in adultsnSevere pain and vesicle, often in chest人类

10、疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学nThe largest genetic content of HHVnVery species specific and cell specific (种属种属和细胞特异性和细胞特异性)nCharacteristic CPE: multinucleated cells and intranuclear inclusionnIs spread cell to cell人类疱疹病毒优秀精美生物医学人类疱疹病毒优秀精美生物医学nSource of infection: patients / inapparent pat

11、ientsn Routes of infectionnClose contactnMother to child人类疱疹病毒优秀精美生物医学nNormal hostnMost are subclinicalnMononucleosis like syndrome (单核细胞增多症单核细胞增多症) a mild illnessnLatent infectionnImmunosuppressed hostnMuch more severenDisseminated pneumonia nIn AIDS: 胃肠炎，视网膜炎胃肠炎，视网膜炎人类疱疹病毒优秀精美生物医学nCongenital and p

12、erinated infection 先天和围产期感染先天和围产期感染nMay be severenFetal and new born nCytomegalic inclusion disease (巨细胞包涵体病巨细胞包涵体病)nDeath of fetusnC.N.S defect : 耳聋，智力发育迟缓耳聋，智力发育迟缓nAn important public problem人类疱疹病毒优秀精美生物医学nViral antigensnLatent phase AgnEBNA1: latent infectionnEBNA2: transformation of cellsnLMP: l

13、atent infection transform cellsnReplication AgnEarly Ag: viral replicationnLate Ag: structure proteins 人类疱疹病毒优秀精美生物医学nSpeared by contact :nPrimary infection: more unapparent nLatent infectionnCell transformation人类疱疹病毒优秀精美生物医学nDiseases nInfectious mononucleosisnTransmitted by infected salivanMore seen in adolescents and young adultsnFever, sore throat, WBC incraesednDistinguish with leukemia heterophil agglutination (异嗜凝集试验异嗜凝集试验)：heterophil Ab can agglutinate sheep RBCnBurkitts lymphomanNasopharyngeal carcinomanImmunodeficient host: 淋巴细胞增殖性疾病淋巴细胞增殖性疾病人类疱