气相层析质谱仪瓦里安科技

1、美商亞洲瓦里安科技美商亞洲瓦里安科技( (股股) )公司公司lgcgc基本原理基本原理lmsms基本原理基本原理l數據處理數據處理 定性定性 & & 定量定量lms tuning ms tuning 與與 日常保養日常保養l應用應用linjector-capillary injectorinjector-capillary injectorlcolumn -capillarycolumn -capillaryldetector-massdetector-masslrecorder-computerrecorder-computerluniversal detectorsflam

2、e ionization detector(fid) for hydrocarbon compounds thermal conductivity detector(tcd)lselective detectorselectron capture detector(ecd)pulsed flame photometric detector(pfpd)thermionic specific detector(tsd)lqualitationretention time same compound has the same r.t. under the same gc conditions.lqu

3、antitationpeak areapeak heightmm+.m+.m/z 15 m/z 57 m/z 27 m/z 43 m/z 29 mass analysisdata recordingplot of bar graph294358ionizationmass analyzerion detectionch3-ch2-ch2-ch3lch3-ch2-ch2-ch3lch3-ch2-ch2-ch3lch3-ch2-ch2-ch3-em/z 58m/z 43m/z 29linlet systemsgc, lc, direct probe inlet.lion sourceei, cil

4、mass analyzermagnetic sector, the quadrupole ion trap.ldetectorelectron multiplier.m+.m/z 15 m/z 57 m/z 27 m/z 43 m/z 29 294358lch4 + e- ch4+ + 2e-lch4+ + ch4 ch3 + ch5+lch5+ + m ch4 + (mh)+ch4 : ci gasm : analytetop endcapbottom endcapring electrodegc column inletfilament assemblyion multiplierdete

5、ctorf folsn/sno or pb/pbo doped glass funnel lshower of electrons is released when an ion strikes the walllthe amplified ion current is sent to the electronic circuit and converted to a voltage signall105107gainion beamslitelectronsto amplifiercascade of electronsresistive conductive surfaceto ampli

6、fier-2kvlretention timelmass spectra of each compoundllibrary searchcompound identificationstructure informationlquantitationrticcquantitation ionlqualitationbackground substractidentification of coelution peaklibrary searchlquantitationbuild calibration curvesample analysis rticc & quantitation

7、 ion180109background correctionbackground correctionaba : before background correctionb : after background correctionlcommercial available library(70 ev)nistwiley libraryweber drug and pesticide libraryluser libraryuser can create library by their own msrequire reliable, tunable ms to produce reprod

8、ucible mass spectralstep i: data reduction lstep ii: presearchlstep iii: main search with full spectrafit reverse fitpuritysamplereferencedifferencesearch resultlmolecular ion formation lmolecular fragmentation c6h5-ch2ch3 + e- c6h5-ch2ch3+(m/z 106) + 2 e-c6h5-ch2ch3 + e- c6h5-ch2+(m/z 91)c6h5-ch2ch

9、3 + e- c6h5+(m/z 77)structuremass spectranamelprepare reference standardslacquire data files for each standardslidentify compounds and retention timeslbuild calibration fileadd points to calibration filesexamine and edit calibration filelexternal standardlinternal standardareaamountsingle point cali

10、brationareaamountmultipoint calibrationresponse=areastandardamountstandardamountunknown=areaunknownresponsesatviewbuild compound tablecompound tabler.t.compound namequantum ioncalibration setupcompound tableresults treatmentabcdefa-r.t.b-compound namec-cas nod-peak areae-resultf-fit, reverse fit, pu

11、ritylto optimize the sensitivity and resolutionlto adjust the relative ion ratiolautotune and manual tunetuning requirementslenvironmentair, water and soil for pesticides, herbicides, semi-volitiles, etc.lpharmaceuticalenantiomers in biologically active compoundslpetroleumcrude oil, gasoline, diesel fuel for pah, additives, etc.lchemicalraw material and syn