嗜热嗜酸菌TABppt课件

1、嗜热嗜酸菌 Thermophilic Acidophilic Bacilli ：TAB主要内容o年Cerny : Isolated TAB from accident products of clear apple juice.o年Darland : Genericly Named B. Acidoterrestris.o年Suzuki : Found Rotten canned low pH commodities odoring in the market, reported D95 (Decimal reduction time at 95)= 2min.o年Niwa : Suggest

2、ed K mediums to isolate TAB,isolating TAB from imported juices.o年Kato : Analyzed elements of offensive smells caused by TAB. Reported the process;Vanillin Guaiacol, aldehydealcohol”.o年Niwa : Developed YSG mediums.o年Matsubara : Reported the way to detect A. Acidoterrestris with PCR.o年Niwa : Reported

3、the way to detect TAB with peroxydase.TAB特性o1.1.菌体菌体( (宽宽 0.70.71m, 1m, 长长3 35m) 5m) 嗜酸耐热菌的菌落形态及显微形态TAB特性o2.2.生长温度范生长温度范(20(2070)70)最适生长温度最适生长温度 : 40: 406060o3.3.生长生长pHpH范围范围(pH 2(pH 26)6)最适最适 pH : 3.5pH : 3.54.54.5TAB来源来源contamination from soil （ （and fruits, fructose） ）SoilSoilSoilSoilSoilSoft dri

4、nksJuiceJuiceHerbMaterialsSoil, Fruits, fructoseSoil, Fruits, fructoseSoil, Fruits, fructoseIn JapanFoundFoundFoundFoundFoundNot FoundNot FoundNot FoundNot FoundNot FoundNot FoundNot FoundNot FoundIsolated fromFrequencySpeciesRareRareRareRareRareRareRareRareRareRareSeveralSeveralManyManyManyManyTAB简

5、介防治饮料中的TAB的方法o提高灭菌温度提高灭菌温度 o UHT sterilization: over F0=1o保持原料和商品低温保持原料和商品低温. 现阶段我们供应商现阶段我们供应商/运输运输/储存是如何做的？储存是如何做的？o 在在 10以下保存冷冻以下保存冷冻 （目的是什么？）（目的是什么？）o控制氧气控制氧气 我公司产品的含氧量控制？我公司产品的含氧量控制？o High risk container：PETmost dangerous）, papero Can Drinks：Control Sealed Oxygen 2ml/350ml.（现在产品含（现在产品含氧量）氧量）o控制每

6、一步条件控制每一步条件.o Plan pH of content under 3.2Effective with Apple or Orange)我们现在原物料我们现在原物料PH：o Use Sodium Benzoate.o Use the ex. from seeds of grapes or other poly-phenols.(with dregs)o We confirm whether odor is with multiplication of TAB.oavailable measures are spread to material suppliers in Japan.

7、TAB的检测方法o嗜热嗜酸菌TAB的检测 .docThe points in planning commoditiesThe points in planning commoditiesWhether would TAB grow upWhether would TAB grow up？（？（Confirm with inoculating Confirm with inoculating PET productsPET products） ）How is the influence of TAB to the commoditiesHow is the influence of TAB to

8、 the commodities？（？（flavor, flavor, appearanceappearance） ）Does the container control O2Does the container control O2？（？（Both PET and Paper Both PET and Paper arent available.arent available.） ）Can we deal as the chilled commoditiesCan we deal as the chilled commodities？The points of controlling mat

9、erialsThe points of controlling materialsWhat materials contain TABWhat materials contain TAB？（？（Juice, Saccharide etc.Juice, Saccharide etc.） ）The materials have to be produced via The materials have to be produced via or removing or removing TAB processes.TAB processes.Dont reuse Water in a loop l

10、ine for washing materials.Dont reuse Water in a loop line for washing materials.How to inspect materialsHow to inspect materials plates, the Peroxidase plates, the Peroxidase methodsmethods） ）Every standard of materialEvery standard of materialThe points of controlling commoditiesThe points of contr

11、olling commoditiesThe commodities are also produced via The commodities are also produced via or removing or removing TAB processes.TAB processes.How to inspect commoditiesHow to inspect commodities plates, and others plates, and others） ）Get sterilizing temperature upGet sterilizing temperature up：

12、Preserve Materials and commodities at low temp.Preserve Materials and commodities at low temp. Chilling distribution ( under 10)Chilling distribution ( under 10)Use Materials with absorption or untransmittance of O2.Use Materials with absorption or untransmittance of O2. Unavailable container: Unava

13、ilable container: ：, Paper, Paper CanCan： ：Control Top of O2 to Control Top of O2 to Plan commodities considering TAB control with contents. Plan commodities considering TAB control with contents. Dropping pH under 3.2Dropping pH under 3.2 effective to Oranges and effective to Oranges and ApplesApples） ） Adding Sodium benzoate.Adding Sodium benzoate. Adding extracts of grape seed or polyphenol powderAdding extracts of grape seed or polyphenol powder sedimentssediments） ） Inoculating TAB and confirm the multiplying in risky Inoculating TAB and confirm the multiplying in risky moditi