第五节定性和定量分析-ppt课件

1、续前续前min%11maxmax，shcmE续前45. 315. 388. 170. 155036127836155036127812AAAAVB，三处最大吸收，定性鉴别：药典规定续前续前%06. 01002101 . 1%/101 . 1100/101 . 1143505. 0334%11肾上腺酮mlmgmlglEACcmlECA定量依据：续前要求单色光前提：iECAmLgCigW 求含样过程：已知稀释)/(/)(lEAmLgCi100/lALmolCi/或%100%样CCii练习mLgmLglEAC/0 .20100/00200. 01207414. 0练习mLgmLgCi/1045. 2

2、100/1045. 21207507. 053mLgC/1050. 2100101001050. 252样%0 .98%1001050. 21045. 2%100%5512样CCBi练习%)0 .764(591. 01001025000.20367255iEAmLmLmLmgmlmLHCL求已知测移取稀至样品稀至吡啶 mLgmLglEACi/1092. 7100/1092. 7764591. 064%0 .99%10025010100100 . 21092. 7%100%26样CCii条件前提：固定仪器和测定CACKA样样同上条件固定条件查得测定样品曲线分别测定配制标准系列过程：CAACA标样

3、标样标样标样AACCAACC例如mLmgmLmLmLmg250 . 32550/200. 0样品标样分别移取续前标样与样品浓度相近；截距为固定仪器和测定条件；前提：0标样和分别测定一定过程：AASiSiAACCsCiCCSiSiAAmm%100%mmii练习mLgCCii/1 .98518. 0508. 01000100000.25105 . 2%1 .98%100%12标示量标示量iCB练习1207508. 01050. 2iiClEACmLgCi/1 .98%1 .98%100%12标示量标示量iCB续前cbaAAAA总定量依据：aaaaaaEACCEA1111由bbbbbbEACCEA2

4、222由bbaaAEAE222111；测定；测定过程：续前babaaaAEEAE2222111；和测定；测定过程：aaaaaaEACCEA1111由bbaababaCECEAAA22222由baababECEAC222续前babababaAEEAEE22221111；和测定；和测定bbaababaCECEAAA11111bbaababaCECEAAA22222bababbabbaaEEEEEAEAC12211221babaabaababEEEEEAEAC12212112babababaAAAAAA222111babbaabababaAAAAAAAAA)()(212121aaAAa2121和的等

5、吸收点选bbbbbbbbaCECEEAAA)(2121bbabbbabEAEEAC21续前bbAAb 2 1 2 1和的等吸收点选aaaaaaabaCECEEAAA)(21 2 1abaaabaaEAEEAC 2 1续前倍率因子令KAAbb21)()()()(1212112212aabbababbababaAKAAKAAAAAKAKAAbA1bA2aaaaabaCEKEAKAA)(1212的干扰消除了bCAaba练习9 .927%11cmEmLgmLgCi/10900. 4100/10900. 419 .927463. 064mLgC/10000. 55052001000.1063样%0 .98%10010000. 510900. 4%100%66样咖啡酸CCi5052001稀释因子注：练习%11cmE12000 .100120001010%11MEcmmLgmLglETlEACi/10165. 3100/10166. 3