各种激发光和吸收光波长

1、各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。这对不可少的参考资料。全部文件请到这里下载！Excitatio n and Emissi on Wavele ngths of FluorophoresTavis FluoroTable: Com mon fluorophores(from Zeiss Corporati on website)CONFOCA 去做多重染色时是必FluorophoreAbsorptio n Emissi on Other info1,5 IAEDANS3364901,8-ANS3724804-Methylumbellifero ne38550

2、25-carboxy-2,7-dichlorofluoresce in5045295-Carboxyfluorescei n (5-FAM)4925185-Carbox yn apthofluoresce in (pH 10)512/598563/668 Ratio Dye, pH5-Carboxytetramethylrhodam ine542568(5-TAMRA)5-FAM (5-Carboxyfluorescei n)4925185-HAT (Hydroxy Tryptami ne)370-415520-5405-Hydroxy Tryptami ne (HAT)370-415520-

3、5405-ROX (carboxy-X-rhodami ne)5786045675915-TAMRA548552(5-Carboxytetramethylrhodam ine)5425686-Carboxyrhodam ine 6G5185436-CR 6G5185436-JOE5205487-Am ino-4-methylcoumari n3514307-Ami noacti no mycin D (7-AAD)5466477-Hydroxy-4-methylcoumari n360449, 4559-Am ino-6-chloro-2-methoxyacridi ne412, 430471

4、,474ABQ344445Acid Fuchs in540630ACMA412, 430471,474(9-Am ino-6-chloro-2-methoxyacridi ne)Acridi ne Orange + DNA502526Acridi ne Orange + RNA460650Acridi ne Oran ge, both DNA & RNA440-480520-650Acrid ine Red455-600560-680Acridi ne Yellow470550Acriflavi n436520Acriflavi n Feulgen SITSA355-425460Aeq

5、uori n (Photoprote in)466PhotoproteinAFPs -AutoFluoresce ntProte in-(Qua ntum Biotech no logies)see sgGFP,sgBFPAlexa Fluor 350346442342441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531,532553, 554Alexa Fluor 546556,557572,573Alexa Fluor 568577, 578603Alexa Fluor 594590, 59461

6、7, 618Alexa Fluor 633632650Alexa Fluor 647647666Alexa Fluor 660668698Alexa Fluor 680679702Alizarin Complex on530-560,580,580624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Ami no methylcoumari n)345425347444AMCA-X353442Amin oacti nomycin D555655Amin ocoumari

7、n346442350445Amin omethylcoumarin (AMCA)345425347444Ani lin Blue600An throcyl stearate360-381446APC (Allophycocya nin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530 Ratio Dye, Zn2+APTS424505Astrazon Brillia nt Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astraz

8、on Yellow 7 GLL450480Atabrine436490ATTO-TAG? CBQCA465560ATTO-TAG? FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9(Bisam in ophe ny loxadiazole)365395BCECF (high pH)492,503520,528BCECF (low pH)482520Berberi ne Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)3

9、81,382, 445, 447, blue shifted GFP (Y66H)Blue Fluoresce nt Prote in383448BlueFluoresce ntProteinBFP/GFP FRETBima ne398490Bisbe nzamide360461Bisbe nzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Bla ncophor FFG390470Blan cophor SV370435BOBO?-1462481BOBO?-3570602Bodipy 492

10、/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodipy Fl504,505511,513Bodipy FL ATP505514Bodi

11、py Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO?-1462481BO-PRO?-3544570Brillia nt Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/4175

12、17/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crims on588, 589611,615Calcium Gree n501,506531Calcium Gree n-1 Ca2+ Dye506531Ca2+ DyeCalcium Gree n-2 Ca2+506/503536Ca2+Calcium Gree n-5N Ca2+506532Ca2+Calcium Gree n-C18 Ca2+509530Ca2+Calcium Orange549575576Calcofluor White385,395, 437, 4

13、40,405445Carboxy-X-rhodami ne (5-ROX)576601Cascade Blue377420398423399Cascade Yellow399550400552Catecholam ine410470CCF2 (Gen eBlazer)CFDA494520CFP - Cya n Fluoresce nt Protein430,433, 474, 475, CyanFluoresce nt436, (453)476,Protein(501)CFP/YFP FRETChlorophyll480650Chromo mycin A436-460470Chromo myc

14、in A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coele nteraz ineCa2+Dye, (429)465Ca2+Dye,biolu min esce neebiolu min esce nee,n ative moleculeCoele nteraz ine cp (Ca2+ Dye,)(430)442Ca2+Dye,biolumi nesce neeCoele nteraz ine f(437)473Ca2+Dye,biolumi nesce neeCoele nteraz ine fcp452C

15、a2+Dye,biolumi nesce neeCoele nteraz ine h(437)464Ca2+Dye,biolumi nesce neeCoele nteraz ine hcp(433)444Ca2+Dye,biolumi nesce neeCoele nteraz ine ip441Ca2+Dye,biolumi nesce neeCoelen teraz ine n(431)467Ca2+biolumi nesce neeDye,Coelen teraz ine O460575Coumarin Phalloidin387470C-phycocyanineCPM Methylc

16、oumari n384469Methylcoumari nCTC400-450602CTC Formaza nCy2489506Cy3.1 8554568Cy3.5581598Cy3514566552570554Cy5.1 8649666Cy5.5675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453) 475 (501)cyclic AMP Fluorose nsor (FiCRhR)500517CyQuant Cell Proliferatio n Assay480520CellProliferatio nAssayDabcyl453Da

17、n syl340578Dan syl Ami ne337517Dan syl Cadaveri ne335518Dan syl Chloride372518Dan syl DHPE336517Dan syl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH(Dichlorodihydrofluorescein505535Diacetate)DDAO463607DHR (Dihydorhodami ne 123)505534Di-4-ANEPPS496705Di-8-ANEPPS

18、 (non-ratio)488605498713DiA (4-Di-16-ASP)456591Dichlorodihydrofluoresce inDiacetate 505535(DCFH)DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DilC18 (5)644665DIDS341415Dihydorhodami ne 123 (DHR)505535DiI (DiIC18 (3)549, 551565Din itrophe nol349DiO (DiOC18(3)484,487501,502DiR748780Lipophilic Tra

19、cerDiR (DiIC18)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopam ine340490-520DsRed558583Red fluoresce nt proteinDTAF494520DY-630-NHS621660Hemicya ne label forprote ins DNAandDY-635-NHS634664Hemicya ne label forprote insDNAandEBFP383447EnhancedBlueFluoresce nt Prote inECFP436474EnhancedCyanF

20、luoresce nt Prote inEGFP488, 498507, 516 EnhancedGreenFluoresce nt Prote inELF 97345530Eosin524545Erythrosi n529, 532554,555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrys inEukoLightEuropium (III) chloride430540EYFP513, 520527, 532 En ha needYellowFlu

21、oresce nt Prote inFast Blue360440FDA494520Feulge n (Pararosa nili ne)570625FIF (Formaldehyd In duced Fluoresce nee)405433FITC490, 494520, 525FITC An tibody493517Flazo Orange375-530612Fluo-3480?506,520,527506Fluo-4494516Fluorescei n (FITC)490, 494520, 525Fluoresce in Diacetate494520Fluoro-Emerald4955

22、24Fluoro-Gold (Hydroxystilbamidi ne)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red?(high pH)572657Fura Red?Fluo-3Fura-2, high calcium335505Excitati on ratio dyeFura-2, low calcium363512Excitati on ratio dyeFura-2/BCECFGen acryl Brillia nt Red B520590Gen acryl Brillia nt Yellow

23、10GF430485Gen acryl Pink 3G470583Gen acryl Yellow 5GF430475Gen eBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wildtype,non-UV excitation475509(wtGFP)GFP wild type, UV excitatio n (wtGFP) 395509GFPuv385508Gloxalic Acid405460Granu lar Blue355425Haematoporphyri n530-560580Hoechst 33258345487Hoechst 33342347Filter sets in stalled on the Axiovert 200M:1) Brightfield