人肌红蛋白基因外显子一的生物信息学分析

1、人肌红蛋白基因外显子一的生物信息学分析一 Introduction：Myoglobin, the article is composed of a peptide chains and a binding protein of heme prosthetic group, is within the muscle oxygen storage protein, its oxygen saturation of the elliptic curve.Myoglobin is the main protein of skeletal muscle and cardiac muscle, when

2、muscle damage, leakage to the circulating in the blood in muscle tissue, increase the serum concentration of myoglobin, the index is used to determine whether muscle damage.二 Purpose：对人肌红蛋白基因外显子一基因序列使用 “Bioedit和DNAMAN、anthe5.0”及相关网站进行GC含量统计、电子酶切、引物设计、基因在人类基因组中的定位、电子克隆及蛋白在细胞内的定位、氨基酸组成统计、分子量、等电点、亲疏水性、

3、信号肽分析（跨膜区域）、二级结构（含保守结构域）及三维结构分析，miR分析，启动子分析等。其中抗原表位分析、多序列比对和进化分析等。三 The nature and function of myoglobin：Nature: myoglobin = a polypeptide chain + a prosthetic group polypeptide chain, composed of 153 amino acid residuesProsthetic group: heme prosthetic groupMolecular weight: 16, 700Function: transp

4、ort of oxygen and oxygen storage function in muscle tertiary structure of myoglobin四 Key word: 肌红蛋白（myoglobin）五 Implements and methods：采用Bioedit和DNAMAN分析软件工具和在线有关蛋白质和核酸方面的分析软件，分别进行核酸和蛋白质两个方面的分析。核酸分析首先从pubmed核酸数据库中搜索到人的Myoglobin核酸序列，分别进行序列的基本性质分析，限制性酶谱分析及其基因在染色体上的定位分析。具体操作方法将根据上述各个软件上“Help”文件进行，基因在染色

5、体上的定位分析根据网页上的操作说明进行。从pubmed蛋白质数据库中搜索到人的Myoglobin和整个前体Myoglobin蛋白质序列，分别进行蛋白序列的基本性质分析（氨基酸组成、分子量、等电点），亲疏水性分析，蛋白酶切位点分析，信号肽序列分析，二级结构和三维结构分析，同源序列和进化分析。二级结构分析包括结构功能域、Motif、保守位点等，具体操作方法将根据上述各个软件上“Help”文件和网页上的操作说明进行。Resluts:1.从NCBI中可以得到一下有关Myoglobi蛋白分子的信息：Human myoglobin gene, exon 1GenBank: M10090

6、.1LOCUS HUMMGI1 2552 bp DNA linear PRI 07-JAN-1995DEFINITION Human myoglobin gene, exon 1.ACCESSION M10090VERSION M10090.1 GI:187580KEYWORDS Fok family repetitive sequence; direct repeat; myoglobin; repeat region; tandem repeat.SEGMENT 1 of 3SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryot

7、a; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.REFERENCE 1 (bases 1 to 2552) AUTHORS Akaboshi,E. TITLE Cloning of the human myoglobin gene JOURNAL Gene 33 (3), 241-249 (1985) PUBMED 2989088COMMENT Ori

8、ginal source text: Human: fetal liver DNA (library of Lawn et al.), clone lambda-HMB151; delta-thalassemia leukocyte DNA (library of Kimura et al.), clone lambda-HMB159. Draft entry and computer-readable copy of sequence 1 kindly provided by E.Akaboshi, 22-OCT-1985. A tandem repeat sequence is found

9、 at position 142-794. Position of the mRNA cap site was deduced from that of the seal myoglobin gene (Blanchetot et al. 1983).FEATURES Location/Qualifiers source 1.2552 /organism="Homo sapiens" /mol_type="genomic DNA" /db_xref="taxon:9606" /map="22q11.2-qter"

10、repeat_region 1100.1750 /note="Fok family repetitive element" prim_transcript 1895.>2552 /note="myoglobin mRNA and intron" gene join(1965.2552,M14602.1:1.2671,M14603.1:1.501) /gene="MB" CDS join(1965.2059,M14602.1:1415.1637,M14603.1:355.501) /gene="MB" /not

11、e="myoglobin" /codon_start=1 /protein_id="AAA59595.1" /db_xref="GI:386872" /translation="MGLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKF DKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKIPV KYLEFISECIIQVLQSKHPGDFGADAEGAMNKALELFRKDMASNYKELGFQG" exon <1965

12、.2059 /gene="MB" /note="myoglobin; G00-119-378" /number=1 intron 2060.>2552 /gene="MB" /note="myoglobin intron A"ORIGIN 2 bp upstream of RsaI site. 1 gtactgtatt ttcattcctc ttagttatct ccctaaaaag actctgagtt ccttgaacac 61 aggaaggtgt tttatttgat tttgttatcc tcagc

13、atgta gcagtgtctg acacacagta 121 ggtgctctat cactgtgaga gggatggatg gatgggtgga gttacagatg gatagaagga 181 tagatggagg gatgggtgga tgatggatgg atagatggat ggagggggga tgatgaatgg 241 agggataatg agtggatgaa tgagggaatg ggtggatgga tggatggagg gatggaggaa 301 cagatagata gatggaggga tgggtgggtg atggatggat agatggatgg agg

14、gagggat 361 gatgaatgga gggataatga atggatgaat gaggggatgg gtggatggat gaatggaggg 421 atgatgggtg gatgaatgaa ttgagggatg gatggatgaa cacatggatg gatggataga 481 tggatagatg gaggaactgg tggattttgg atggatgggt ggatggatag atgaatgaat 541 gcctggatag acaaagagat gatggataga tgaatagatg aattaaggga tgtcggatag 601 atggaggg

15、at tgatagatgt tggatggatg ggtggtggat ggatagatga gtgaatgcat 661 ggatagacaa agagatgatg gatggatgaa ttaagggatg acagatggat ggatggatga 721 gtaactggat ggacaagtgg ataaatggat agatggttga atacctgaat ggattgaagg 781 aggatgcatg gatgtaagat aaggctaatc atcctccact ctctttcttt gcaaaaccat 841 ccacccattt actcaataaa cattta

16、ttca gttcaaactt ggcacaaagc accatgtgag 901 gcccaagaga tacgtgggtt aataaaacag agctcctgcc ctcctgaaaa ctgcaaagaa 961 aggggcgtgg cttcctgagt tcaaatccca actctgccag cgactagctg tacatcagtg 1021 atgtttccct actttctctc aattaaatag ggataatgtc agtacctatc acattgggag 1081 gtcttgcggg gattaaatga gttaccaaat gccaagtgtt tg

17、ggacaggg cctggcaccc 1141 agcaaagtct cttgtgagtg ctggctgcta ttatcctaat ggagaagatg gcatgaaaac 1201 caggaaatag gatgcccttt gggaagcaat gcaacaggaa cttacacaaa gaaaggaaag 1261 gaggaagcaa ttagtggtgt ctcaaaggag tatgtcaaga aaaacttttc agagggaaac 1321 ctttgagcag ggtcatgaaa acaggagttc tctaagagat tgtggacttg cctggga

18、cca 1381 cctggctata agcacaaaac catccggttc ctttctgtca cttctggcgg gtgaggggtc 1441 tctggcaaag gggcagaagg tgcgtgagag gttgcgaatg gccaggactg tcctggggcc 1501 agccggggca cctggtggcc aagcttagaa acatgacagg tcctcttggg agggctgacc 1561 gcagggagcg ttgggtttca ggctgctggc gtcggcttct gtggtgccct ttctgtcggc 1621 tatgaga

19、gtc cagacagtgc ccaacctcct ccccttcttt ccacacgcac aaccacccca 1681 ccccctgtgg cctgagctgt cctgcctcgc cacaatggca cctgccctaa aatagcttcc 1741 catgtgaggg ctagagaaag gaaaagatta gaccctccct ggatgagaga gagaaagtga 1801 aggagggcag gggaggggga cagcgagcca ttgagcgatc tttgtcaagc atcccagaag 1861 gtataaaaac gcccttggga c

20、caggcagcc tcaaacccca gctgttgggg ccaggacacc 1921 cagtgagccc atacttgctc tttttgtctt cttcagactg cgccatgggg ctcagcgacg 1981 gggaatggca gttggtgctg aacgtctggg ggaaggtgga ggctgacatc ccaggccatg 2041 ggcaggaagt cctcatcagg taaaaggaag agattccatt gcccctgcca cccacaccct 2101 aagatcaagg gtgttcagct gcaaggtgga aagttt

21、gcac gtggggtagg tcagttggct 2161 gcattagtta agggtgttag aacggtcact tgctttttct ttgcttttaa gtgtcaggga 2221 ttggactcag gagagggaaa ggagccattt caggctgatg tcagcagctg gaggaagcat 2281 gagaatcaaa cctaggatgc tcagagtcca ccaggaagaa ttttagaatt atagacagtc 2341 agagttaaca agggtcctga gagattttgt acagccacct ctcttacagg

22、atgaggacaa 2401 aaagcgactg agaaggggag gacatttcca gagtcacagc tcattaaatg ctcttaaagt 2461 gtcaaggtta agacatgctc ttcaagggga gacagatctg gttctagact tggctctgcc 2521 actgagccac tgggtgacct ttgggaaggt acOfficial Symbol MBprovided by HGNCOfficial Full Name myoglobinprovided by HGNCPrimary source :HGN

23、C:6915Locus tag :RP4-569D19.7Seerelated：Ensembl:ENSG; HPRD:01170; MIM:160000; Vega:OTTHUMGGene type:protein coding Refsep status:REVIEWEDOrganism：Homo sapiens Lineage： Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Hap

24、lorrhini; Catarrhini; Hominidae; HomoAlso known as：PVALBGenomic contextLocation:22q13.1Sequence:Chromosome: 22; NC_000022.10 (36002811.36019401, complement)See MB in Epigenomics, MapViewerChromosome 22 - NC_000022.10Genomic regions, transcripts, and productsGenomicSequence                                                   Go to nucleotideGraphic