盐酸石蒜碱作用机制 - Medchemexpress - MCE中国

1、Product Data SheetLycorine hydrochlorideCat. No.: HY-N0289CAS No.: 2188-68-3分式: CHClNO分量: 323.77作靶点: Autophagy; Bacterial; Virus Protease作通路: Autophagy; Anti-infection储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 25 mg/mL (77.22 mM; Need ultrasonic)SolventMas

2、s1 mg 5 mg 10 mgConcentration制备储备液1 mM 3.0886 mL 15.4431 mL 30.8861 mL5 mM 0.6177 mL 3.0886 mL 6.1772 mL10 mM 0.3089 mL 1.5443 mL 3.0886 mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存式和期限：-80C, 6 months; -20C, 1 month。-80C 储存时，请在 6 个内使，-20C 储存时，请在 1 个内使。体内实验请根据您的实验动物和给药式选择适当的溶解案。以下溶解

3、案都请先按照 In Vitro 式配制澄清的储备液，再依次添加助溶剂：为保证实验结果的可靠性，澄 的储备液可以根据储存条件，适当保存；体内实验的作液，建议您现现配，当天使； 以下溶剂前显的百分 指该溶剂在您配制终溶液中的体积占；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的式助溶1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.08 mg/mL (6.42 mM); Clear solution此案可获得 2.08 mg/mL (6.42 mM，饱和度未知) 的澄清溶液。以 1 mL 作液为例

4、，取 100 L 20.8 mg/mL 的澄 DMSO 储备液加到 400 L PEG300 中，混合均匀；向上述体系中加50 L Tween-80，混合均匀；然后继续加 450 L 理盐定容 1 mL。2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.08 mg/mL (6.42 mM); Clear solution此案可获得 2.08 mg/mL (6.42 mM，饱和度未知) 的澄清溶液。Page 1 of 2 www.MedChemE以 1 mL 作液为例，取 100 L 20.8 mg/mL 的澄均匀。DM

5、SO 储备液加到 900 L 20% 的 SBE-CD 理盐溶液中，混合3. 请依序添加每种溶剂： 10% DMSO 90% corn oilSolubility: 2.08 mg/mL (6.42 mM); Clear solution此案可获得 2.08 mg/mL (6.42 mM，饱和度未知) 的澄 溶液，此案不适于实验周 期在半个以上的实验。以 1 mL 作液为例，取 100 L 20.8 mg/mL 的澄 DMSO 储备液加到 900 L 油中，混合均匀。BIOLOGICAL ACTIVITY物活性 Lycorine hydrochloride 蒜的主 活性成分，也 素瘤管成抑制剂

6、，具有抗肿瘤活性。Lycorine hydrochloride 能有效抑制 Hey1B 细胞的有丝分裂增殖 (IC50 为 1.2 M)。IC & Target IC50: 1.2 M (Hey1B cells )2体外研究 Lycorine hydrochloride (0-25 M; 48 hours; C8161 cells) treatment markedly suppresses the expression of vascularendothelial (VE)-cadherin in a dose-dependent manner and also slightly dimin

7、ishes the expression of Sema4D inC8161 cells. However, the expression of the other six genes is not affected1.Lycorine hydrochloride (0-25 M; 48 hours; C8161 cells) treatment markedly reduces VE-cadherin protein levels inC8161 cells in a dose-dependent fashion1.RT-PCR1Cell Line: C8161 cellsConcentra

8、tion: 0 M, 1.56 M, 3.13 M, 6.25 M, 12.5 M, 25 MIncubation Time: 48 hoursResult: Markedly suppressed the expression of VE-cadherin in a dose-dependent mannerand also slightly diminished the expression of Sema4D in C8161 cells.Western Blot Analysis1Cell Line: C8161 cellsConcentration: 0 M, 1.56 M, 3.1

9、3 M, 6.25 M, 12.5 M, 25 MIncubation Time: 48 hoursResult: Markedly reduced VE-cadherin protein levels in C8161 cells in a dose-dependentfashion.体内研究Lycorine hydrochloride (0-15 g; subcutaneous injection; for 14 days; BALB/C nude mice) treatment has an inhibitoryeffect on melanoma cell-mediated tumor

10、 vasculogenic mimicry1.Animal Model: 6- to 8-week-old BALB/C nude mice1Dosage: 0 g, 7.5 g, 15 gAdministration: Subcutaneous injection; for 14 daysPage 2 of 3 www.MedChemEResult: Showed an inhibitory effect on melanoma cell-mediated tumor vasculogenic mimicry.REFERENCES1. Liu, R. et al. Lycorine hydr

11、ochloride inhibits metastatic melanoma cell-dominant vasculogenic mimicry. Pigment cell & melanoma research 25, 630-638,doi:10.1111/j.1755-148X.2012.01036.x (2012).2. Cao, Z. et al.Lycorine hydrochloride selectively inhibits human ovarian cancer cell proliferation and tumor neovascularization with very low toxicity.Toxicology letters 218, 174-185, doi:10.1016/j.toxlet.2013.01.018 (2013).McePdfHeightCaution: Product has not been fully vali