Procyanidin-B1 - Toll-like Receptor (TLR) Antagonist - 生命科学试剂 - MedChemExpress

1、Hotline: 400-820-3792Inhibitors Agonists Screening Librarieswww.MedChemEProcyanidin B1Cat. No.: HY-N0795CAS No.: 20315-25-7分式: CHO分量: 578.52作靶点: Toll-like Receptor (TLR)作通路: Immunology/Inflammation储存式: Powder -20C 3 years4C 2 yearsIn solvent -80C 6 months-20C 1 month溶解性数据体外实验 DMSO : 25 mg/mL (43.21

2、mM; Need ultrasonic)Mass Solvent1 mg 5 mg 10 mg Concentration制备储备液1 mM 1.7285 mL 8.6427 mL 17.2855 mL5 mM 0.3457 mL 1.7285 mL 3.4571 mL10 mM 0.1729 mL 0.8643 mL 1.7285 mL请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液，并请注意储备液的保存式和期限。体内实验 请根据您的实验动物和给药式选择适当的溶解案，配制前请先配制澄清的储备液，再依次添加助溶剂(为保证实验结果的可靠性，体内实验的作液，建议您现现配，当天使；澄清的储备

3、液可以根据储存条件，适当保存；以下溶剂前的百分 指该溶剂在您配制终溶液中的体积占)：1. 请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% salineSolubility: 2.5 mg/mL (4.32 mM); Clear solution2. 请依序添加每种溶剂： 10% DMSO 90% (20% SBE-CD in saline)Solubility: 2.5 mg/mL (4.32 mM); Clear solutionBIOLOGICAL ACTIVITY1/2 Master of Small Molecules 您边的抑制剂师www

4、.MedChemE物活性 Procyanidin B1种多酚类 酮物质，存在于常见的果中，能够与 TLR4/MD-2 复合体结合，具有抗炎活性。体外研究 Procyanidin B1 is a polyphenolic flavonoid isolated from fruits and fruit juices, binds to TLR4/MD-2 complex,and has anti-inflammatory activity. Procyanidin B1 causes cellular toxicity at concentrations 100 g/mL.Procyanidin

5、 B1 (100 g/mL) inhibits LPS-induced TNF- production, and expression of MD-2, TRAF6, NF-BmRNA, phosphorylated p38 MAPK and NF-B protein in THP1 cells 1. Procyanidin B1 (50-100 M) protectsagainst A oligomer-induced neuronal death. Procyanidin B1 potently inhibits the activation of caspase-3 at100 M, c

6、aspase-8 at concentrations of 30, 50, and 100 M and caspase-9 at concentrations of 10, 30, 50,and 100 M 2. Procyanidin B1 (10, 20, 30 M) significantly and dose-dependently induces expression ofACO and CPT1, with no obvious effect on mRNA expression of PPAR 3.PROTOCOLCell Assay 1 To investigate the c

7、ytotoxic effect of Procyanidin B1, viability of THP1 cells is assessed using CCK8 assay.THP1 cells are treated with Procyanidin B1 for 18 h, and 10 L of CCK8 solution is then added to each welland the cultures are incubated for 4 h at 37C. The optical density (OD) at 450 nm is measured using anELx80

8、8 Absorbance Microplate Reader. The Procyanidin B1 concentration tested ranges from 50 to 200 g/mL. Each sample is tested in triplicate 1.MCE has not independently confirmed the accuracy of these methods. They are for reference only.REFERENCES1. Xing J, et al. Anti-inflammatory effect of procyanidin

9、 B1 on LPS-treated THP1 cells via interaction with the TLR4-MD-2 heterodimer andp38 MAPK and NF-B signaling. Mol Cell Biochem. 2015 Sep;407(1-2):89-95.2. Kanno H, et al. Protective effects of glycycoumarin and procyanidin B1, active components of traditional Japanese medicineyokukansan, on amyloid o

10、ligomer-induced neuronal death. J Ethnopharmacol. 2015 Jan 15;159:122-8.3. Shimada T, et al. Flavangenol (pine bark extract) and its major component procyanidin B1 enhance fatty acid oxidation in fat-loadedmodels. Eur J Pharmacol. 2012 Feb 29;677(1-3):147-53.McePdfHeightCaution: Product has not been fully valida