Betaine-Trimethylglycine-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEBetaineCat.No.:HY-B0710CASNo.:107-43-7Synonyms:Trimethylglycine分⼦式:C₅H₁₁NO₂分⼦量:117.15作⽤靶点:EndogenousMetabolite作⽤通路:MetabolicEnzyme/Protease储存⽅式:4°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验H2O:100mg/mL(853.61mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM8.5361mL42.6803mL85.3606mL5mM1.7072mL8.5361mL17.0721mL10mM0.8536mL4.2680mL8.5361mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。BIOLOGICALACTIVITY⽣物活性Betaine(Trimethylglycine)许多⾷物中发现的天然化合物，也⼀种可维持正常DNA甲化模式的活性甲供体。Betaine⼴泛存在于植物，动物，微⽣物和丰富的膳⾷来中，包括海鲜，菠菜和麦麸。Betaine也可作为渗透物，通过防⽌脱⽔和渗透失活来维持禽类的细胞⽔和离⼦平衡，从⽽提⾼禽类对热应激的能⼒。它有助于维持保护性渗透活性，特别在热应激的鸟类中。Betaine可以促进各种肠道微⽣物抵抗渗透变化，从⽽改微⽣物发酵活性。1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEIC50&TargetHumanEndogenousMetaboliteMicrobialMetabolite体外研究Betaine(Trimethylglycine)inhibitesproliferationofHeLacellssignificantly[1].体内研究Betaine(Trimethylglycine)attenuatesliver-to-bodyweightratio[2].PROTOCOLCellAssay[1]CytotoxiceffectsofBetaineareevaluatedbyMTTassays.Briefly,5×103HeLaorMCF-10Acells/wellareseededina96-wellplateandculturedovernight.Then,differentconcentrationsofBetaine(from0to100mg/mL)areaddedtoeachwell,andtheMTTassayisperformedat24,48,72and96hafterincubation.Cu/Zn-SODactivitiesaredetectedusingthesuperoxidedismutaseAssayKitaccordingtothemanufacturer’sinstructions[1].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.AnimalMaleC57BL/6miceweighing25±0.5gareusedinthisstudy.AnimalsarerandomlyassignedtofourgroupsAdministration[2](n=6to8pergroup)andpair-fedamodifiedLieber-DeCarlialcohol(AF)orisocaloricmaltosedextrincontrolliquiddiet(PF)for5weekswithastepwisefeedingprocedure.Theethanolcontentstartsat30%oftotalcaloriesandgraduallyincreasesupto36%inthelastweek.Betaineisadministeredasasupplementintheliquiddiets(bothPFandAF)ataconcentrationof0.5%(w/v),simultaneouslywiththealcohol.Allanimalshaveaccesstodietandwateradlibitum.Attheendoftheexperiment,themicearekilledafterbeingdeprivedoffoodfor4ꢀh,andtheplasma,liverandepididymalfatpadsamplesareharvestedforassays[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.REFERENCES[1].GuoY,etal.BetaineEffectsonMorphology,Proliferation,andp53-inducedApoptosisofHeLaCervicalCarcinomaCellsinVitro.AsianPacJCancerPrev.2015;16(8):3195-201.[2].DouX,etal.Rectificationofimpairedadiposetissuemethylationstatusandlipolyticresponsecontributestohepatoprotectiveeffectofbetaineinamousemodelofalcoholicliverdisease.BrJPharmacol.2014Sep;171(17):4073-86.[3].SaeedM,etal.Reconsideringbetaineasanaturalanti-heatstressagentinpoultryindustry:areview.TropAnimHealthProd.2017Oct;49(7):1329-1338.McePdfHeightCaution:Producthasnotbeenfully