BNS-22-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEBNS-22Cat.No.:HY-122594CASNo.:1151668-24-4分⼦式:C₂₄H₂₅NO₅分⼦量:407.46作⽤靶点:Topoisomerase作⽤通路:CellCycle/DNADamage储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性BNS-22⼀种DNA拓扑异构酶II(TOP2)催化抑制剂，作⽤于⼈的TOP2α和TOP2β的IC50值分别2.8μM和0.42μM。BNS-22可诱导分裂异常，具有抗增殖活性。IC50&TargettopoisomeraseIIalphatopoisomeraseIIbeta2.8μM(IC50,inhuman)0.42μM(IC50,inhuman)体外研究BNS-22(0-30μM,24-48h)caninhibitcellgrowthandinfluenceoncellcycleprogressionofthehumancervicalepidermoidcarcinomacelllineHeLainadose-dependentmanner[1].BNS-22(0-30μM,0-6h)doesnotinduceDNAdamageandhasanantagonisticeffectonTOP2toxin-mediatedDNAdamage[1].CellProliferationAssay[1]CellLine:HeLacelllinesConcentration:0-30μMIncubationTime:24h,48hResult:InhibitedcellgrowthwiththeIC50valueof4.9μMafter24hoursand1.0μMafter48hours.CellCycleAnalysis[1]CellLine:HeLacelllines1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemEConcentration:0-30μMIncubationTime:24hResult:IncreasednumberofcellsinG2/Mphase.Disruptedmitoticspindleformationandinducedpolyploidcellformationat3μM.WesternBlotAnalysis[1]CellLine:HeLacelllinesConcentration:0-30μMIncubationTime:0,30min,1h,2h,4h,6hResult:Decreasedetoposide-inducedγ-H2AXexpressionanddecreasedtotalcellularTOP2βlevels,butnotTOP2αlevels.REFERENCES[1].MakotoKawatani,etal.Identificationofasmall-moleculeinhibitorofDNAtopoisomeraseIIbyproteomicprofiling.ChemBiol.2011Jun24;18(6):743-51.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearchuseonly.Tel:400-820-3792;0