5-BrdU-BrdU-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemE5-BrdUCat.No.:HY-15910CASNo.:59-14-3Synonyms:BrdU;5-Bromo-2'-deoxyuridine;BUdR分⼦式:C₉H₁₁BrN₂O₅分⼦量:307.1作⽤靶点:NucleosideAntimetabolite/Analog作⽤通路:CellCycle/DNADamage储存⽅式:4°C,protectfromlight*Insolvent:-80°C,6months;-20°C,1month(protectfrom

light)溶解性数据体外实验DMSO:250mg/mL(814.07mM;Needultrasonic)H2O:2mg/mL(6.51mM;ultrasonicandwarmingandheatto60°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM3.2563mL16.2813mL32.5627mL5mM0.6513mL3.2563mL6.5125mL10mM0.3256mL1.6281mL3.2563mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(protectfromlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%saline1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemESolubility:≥2.08mg/mL(6.77mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.08mg/mL(6.77mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(6.77mM);Clearsolution4.请依序添加每种溶剂：PBSSolubility:16.25mg/mL(52.91mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性5-BrdU(BrdU)⼀种核苷类似物，可与胸苷竞争掺⼊DNA。5-BrdU通常⽤于检测增殖细胞。体外研究Bromodeoxyuridineinducesaprogressive,dose-responsivesuppressionofcancercelllineandcancerstemcellpopulationexpansionRG2ratgliomacells.InH9cellsandBJfibroblasts,bromodeoxyuridinealtersthecellcycleprofile[1].BrdUisstablyintegratedintotheDNA,andthuscanbeusedinassessmentofcellproliferationandothercellprocession[2].体内研究Bromodeoxyuridine(300mg/kg,i.p.or0.8mg/mL,p.o.)significantlyslowstumorprogressioninratgliomaRG2tumormodel[1].PROTOCOLCellAssay[1]Culturesareinitiallyplatedat2000cells/cm2andarequantifiedwithaZ2CoulterCounter.RG2ratgliomacellsaretreatedoncewith0,1,10,or50µMBrdUfor24hours,andcumulativegrowthcurvesareobtainedover18days.Controlandtreatedcellsarequantifiedandreplatedatequaldensitiesondays5,12,and18aftertreatment.MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•CellRes.2020Sep;30(9):732-744.•CellRes.2019Jan;29(1):23-41.•NatCancer.2022Jun30.•NatCommun.2022Jan10;13(1):182.•NatureMetabolism.2021May;3(5):682-700.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].LevkoffLH,etal.Bromodeoxyuridineinhibitscancercellproliferationinvitroandinvivo.Neoplasia.2008Aug;10(8):804-16.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE[2].RothaeuslerK,etal.Assessmentofcellproliferationby5-bromodeoxyuridine(BrdU)labelingformulticolorflowcytometry.CurrProtocCytom.2007Apr;Chapter7:Unit7.31McePdfHeightCaution:Producthasnotbeenfullyvalidated