Adenosine-5-diphosphoribose-sodium-ADP-ribose-sodium-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEAdenosine5′-diphosphoribosesodiumCat.No.:HY-100973ACASNo.:68414-18-6Synonyms:ADPribosesodium分⼦式:C₁₅H₂₂N₅NaO₁₄P₂分⼦量:581.3作⽤靶点:TRPChannel;Autophagy作⽤通路:MembraneTransporter/IonChannel;NeuronalSignaling;Autophagy储存⽅式:-20°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验H2O:125mg/mL(215.04mM;Needultrasonic)DMSO:25mg/mL(43.01mM;ultrasonicandwarmingandheatto80°C)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.7203mL8.6014mL17.2028mL5mM0.3441mL1.7203mL3.4406mL10mM0.1720mL0.8601mL1.7203mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：PBSSolubility:100mg/mL(172.03mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性Adenosine5′-diphosphoribosesodium(ADPribosesodium)⼀种烟酰胺腺嘌呤核苷酸(NAD+)的代谢产物，也⼀种最有效和最主的细胞内Ca2+渗透性阳离⼦TRPM2通道激活剂，还可以增强⾃噬(autophagy)。IC50&TargetTRPM2channel[1][2]Autophagy[1]体外研究Inmouseembryonicfibroblasts(MEFs),H2O2treatmentdemonstratesthattheactivationofpoly(ADP-ribose)(PAR)polymerase-1(PARP-1)producedAdenosine5′-diphosphoribose(ADPribose),whichisanactivatingsignalforTRPM2channels,therebypromotingCa2+elevationthroughextracellularCa2+influxand(or)lysosomalCa2+release.Thisprocesseventuallyactivatesearlyorlateautophagyinresponsetodifferentdegreesofoxidativestress[1][1].TRPM2channelsareactivatedbybindingofAdenosine5′-diphosphoribose(ADPribose)totheintracellularNUDT9-homology(NUDT9-H)domainuniquetoTRPM2andlocatedatitsCterminus.InadditiontoADPR,intracellularCa2+isanessentialcoactivator:TRPM2channelsopenonlyinthecombinedpresenceofbothligands[2].REFERENCES[1].ZhangDX,etal.ThepotentialregulatoryrolesofNAD(+)anditsmetabolisminautophagy.Metabolism.2016Apr;65(4):454-62.[2].TóthB,etal.PorecollapseunderliesirreversibleinactivationofTRPM2cationchannelcurrents.ProcNatlAcadSciUSA.2012Aug14;109(33):13440-5.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.For