Calmidazolium-chloride-R-24571-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemECalmidazoliumchlorideCat.No.:HY-103319CASNo.:57265-65-3Synonyms:R24571分⼦式:C₃₁H₂₃Cl₇N₂O分⼦量:687.7作⽤靶点:CaMK;Autophagy作⽤通路:NeuronalSignaling;Autophagy储存⽅式:-20°C,sealedstorage,awayfrommoisture*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoisture)溶解性数据体外实验DMSO:100mg/mL(145.41mM;Needultrasonic)MassSolvent1mg5mg10mgConcentration制备储备液1mM1.4541mL7.2706mL14.5412mL5mM0.2908mL1.4541mL2.9082mL10mM0.1454mL0.7271mL1.4541mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoisture)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.08mg/mL(3.02mM);Clearsolution1/2MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE2.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.08mg/mL(3.02mM);ClearsolutionBIOLOGICALACTIVITY⽣物活性Calmidazoliumchloride(R24571)⼀种钙调蛋⽩拮抗剂，拮抗CaM依赖性磷酸⼆酯酶和钙调蛋⽩诱导的红细胞Ca2+转运ATP酶活化，IC50分别为0.15和0.35μM[1]。也⽤于抗癌研究[2]。它结合CaMK的Kd值为3nM[3]。IC50&TargetKd:3nM(Calmodulin)[3]体外研究Calmidazoliumchlorideiswidelyusedasacalmodulin(CaM)antagonist,butisalsoknowntoinduceapoptosisincertaincancercelllines.Calmidazoliumchloride(3,5,7,10μM,30minutes-24hours)inhibitsgrowthofmouseF9ECCs[2].CellViabilityAssay[2]CellLine:MouseF9ECCsConcentration:3,5,7,10μMIncubationTime:30minutes-24hoursResult:TheIC50sofCalmidazoliumchloridetreatedF9ECCsandE14ESCsare8.18μM,and12.69μM[2].REFERENCES[1].GietzenK,etal.Comparisonofthecalmodulinantagonistscompound48/80andcalmidazolium.BiochemJ.1983Dec15;216(3):611-6.[2].LeeJ,etal.Calmidazoliumchlorideinhibitsgrowthofmurineembryonalcarcinomacells,amodelofcancerstem-likecells.ToxicolInVitro.2016Sep;35:86-92.[3].BuduA,etal.CalmidazoliumevokeshighcalciumfluctuationsinPlasmodiumfalciparum.CellSignal.2016Mar;28(3):125-135.McePdfHeightCaution:Producthasnotbeenfullyvalidatedformedicalapplications.Forresearch