Gadopentetate-dimeglumine-SH-L-451A-DataSheet-生命科学试剂-MedChemExpress

Hotline:400-820-3792Inhibitors•ScreeningLibraries•Proteinswww.MedChemEGadopentetatedimeglumineCat.No.:HY-A0167CASNo.:86050-77-3Synonyms:SH-L-451A;Gadopenteticaciddimegluminesalt分⼦式:C₂₈H₅₄GdN₅O₂₀分⼦量:938作⽤靶点:Others作⽤通路:Others储存⽅式:4°C,sealedstorage,awayfrommoistureandlight*Insolvent:-80°C,6months;-20°C,1month(sealed

storage,awayfrommoistureandlight)溶解性数据体外实验DMSO:100mg/mL(106.61mM;Needultrasonic)H2O:≥100mg/mL(106.61mM)*"≥"meanssoluble,butsaturationunknown.MassSolvent1mg5mg10mgConcentration制备储备液1mM1.0661mL5.3305mL10.6610mL5mM0.2132mL1.0661mL2.1322mL10mM0.1066mL0.5330mL1.0661mL请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；⼀旦配成溶液，请分装保存，避免反复冻融造成的产品失效。储备液的保存⽅式和期限：-80°C,6months;-20°C,1month(sealedstorage,awayfrommoistureandlight)。-80°C储存时，请在6个⽉内使⽤，-20°C储存时，请在1个⽉内使⽤。体内实验请根据您的实验动物和给药⽅式选择适当的溶解⽅案。以下溶解⽅案都请先按照InVitro⽅式配制澄的储备液，再依次添加助溶剂：(为保证实验结果的可靠性，澄的储备液可以根据储存条件，适当保存；体内实验的⼯作液，建议您现⽤现配，当天使⽤；以下溶剂前显⽰的百分⽐指该溶剂在您配制终溶液中的体积占⽐；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的⽅式助溶)1/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE1.请依序添加每种溶剂：10%DMSO>>40%PEG300>>5%Tween-80>>45%salineSolubility:≥2.5mg/mL(2.67mM);Clearsolution2.请依序添加每种溶剂：10%DMSO>>90%(20%SBE-β-CDinsaline)Solubility:≥2.5mg/mL(2.67mM);Clearsolution3.请依序添加每种溶剂：10%DMSO>>90%cornoilSolubility:≥2.5mg/mL(2.67mM);Clearsolution4.请依序添加每种溶剂：SalineSolubility:50mg/mL(53.30mM);Clearsolution;NeedultrasonicBIOLOGICALACTIVITY⽣物活性Gadopentetatedimeglumine(SH-L-451A)⽤于核磁共振成像(MRI)中，更晰地看到⾎管，器官和其他⾮⾻组织。体外研究Gadobenatedimeglumine-enhancedMRIsignificantlyimprovescancerdetectioncomparedtogadopentetatedimeglumine-enhancedMRI,mammography,andultrasoundinaselectedgroupofpatientsundergoingbreastMRIforpreoperativestagingorbecauseofinconclusivefindingsatconventionalimaging[1].InterstitialMRlymphographywithgadopentetatedimeglumine(Gd-DTPA)orgadoxetatedisodium(Gd-EOB-DTPA)allowsclearvisualizationofthelymphaticpathwayinhealthymice,andnosignificantdifferenceisfoundbetweenthetwoagents.Theirrapidkineticslimitstheimagingtimingwindow,however,facilitatesrepeatedassessmentinasingleimagingsession[2].Directvisualizationofspilledgastrointestinalgadopentetatedimegluminehelpsdiscriminateischemicfromcontrolratsinthismodel[3].PROTOCOLAnimalRats:Twenty-eightratsareanesthetizedandmidlinelaparotomyisperformed.AnimalsaredividedintofourAdministration[2][3]groups:control,ligationofasinglemesentericarcade,ligationofsixconsecutivearcades,andligationoftheanteriormesentericartery(analogoustothesuperiormesentericarteryinhumans).A1.0-mLentericbolusofgadopentetatedimegluminedilutedwithsterilewater(1:1)isgivenviagavage.Magneticresonanceimagingisperformed2hrafterlaparotomyandreviewedforthepresenceofintraperitonealgadopentetatedimegluminebytwoexperiencedobservers.Animalsaresacrificed24hraftersurgeryforpathologicexamination[3].Mice:MRlymphographyareperformedafterthesubcutaneousinjectionofgadopentetatedimeglumine(Gd-DTPA)orgadoxetatedisodium(Gd-EOB-DTPA)(0.1,0.5,or2.0mmolpermouse)intotherightfootpadinsixhealthymice,andthetimecoursesofcontrastenhancementareassessed.Additionally,thelymphaticpathwaysfromtwodistinctsitesareassessedintandembyinterstitialMRlymphographystudies[2].MCEhasnotindependentlyconfirmedtheaccuracyofthesemethods.Theyareforreferenceonly.户使⽤本产品发表的科研⽂献•Biomaterials.2019Mar;197:207-219.2/3MasterofBioactiveMolecules—您⾝边的抑制剂⼤师www.MedChemE•ACSOmega.2020Oct7;5(41):26655-26666.Seemorecustomervalidationsonwww.MedChemEREFERENCES[1].GilbertFJ,etal.Comparisonofgadobenatedimeglumine-enhancedbreastMRIandgadopentetatedimeglumine-enhancedbreastMRIwithmammographyandultrasoundforthedetectionofbreastcancer.JMagnResonImaging.2014May;39(5):1272-86.[2].ShengF,etal.InterstitialMRlymphographyinmicewithgadopentetatedimeglumineandgadoxetatedisodium.JMagnResonImaging.2011Feb;33(2):490-7.[3].MontalbanoJM,etal.Magneticresonanceimagingdetectionofextraluminalenterallyadministeredgadopentetatedimeglumineinaratmodelofintestinalischemia.AcadRadiol.1996Jun;3(6